Osteogenic and antibacterial dual functions of a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/ polyurethane composite scaffold

作     者：Zhiping Kuang Guangming Dai Ruijie Wan Dongli Zhang Chen Zhao Cheng Chen Jidong Li Hongchen Gu Wei Huang 

作者机构：Department of Orthopedic SurgeryThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Orthopaedic SurgeryChongqing Traditional Chinese Medicine HospitalChongqing400011PR China Research Center for Nano-BiomaterialsAnalytical&Testing CenterSichuan UniversityChengduSichuan Province610065PR China State Key Laboratory of Oncogenes and Related GenesSchool of Biomedical EngineeringMed-X Research InstituteShanghai Jiaotong UniversityShanghai200240PR China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2021年第8卷第2期

页      面：193-202页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This research was funded by the National High Technology Research and Development Program of China(863 Program grant number 2013AA032203) 

主　　题：Antibacterial Composite scaffold Levofloxacin Nanobiomaterial Osteogenesis 

摘      要：Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic *** have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in ***,the potential mechanisms of antibacterial and osteogenic induction properties remain ***,for osteogenesis property,immunohistochemistry,PCR,and Western blot were performed to detect the expression of osteogenic ***,flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and *** antibacterial property,the material was co-cultivated with bacteria,bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion ***,scanning electron microscope was applied to observe adhesion of *** terms of osteogenic induction,we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/***/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1a1,as well as effectively promoted the transition from G1 phase to G2 ***,Lev/MSNs/n-HA/PU could reduce apoptosis of ***,both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies,while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities,and lower bacterial adhesion than n-HA/*** results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility in vitro,which induce osteogenic differentiation of MSCs,promote proliferation and differentiation of MC3T3-E1,and inhibit ***,clear in vitro antibacterial effect of Lev/MSNs/n-HA/PU was also *** summary,this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application.