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Piezoelectric film promotes skin wound healing with enhanced collagen deposition and vessels regeneration via upregulation of PI3K/AKT

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Nano Research 2024年第8期17卷 7461-7478页

作者： Qi Xu Wufei Dai Peizhe Li Qinglin Li Zhen Gao Xiaoli Wu Wei Liu Wenbo Wang Department of Plastic and Reconstructive Surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Research Institute of Plastic Surgery Shandong Second Medical UniversityWeifang 261000China Department of Surgery Air Force Medical CenterPLABeijing 100142China

Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric *** stimulation plays a pivotal role in the restorative processes of skin injuries,encompassing collagen deposition,angiogenesis,inflammation,and *** electrical stimulation therapy replicates and enhances the effects of endogenous wound electric fields by applying an external electric field to the wound site,thereby promoting skin wound *** this study,we developed a self-powered repetitive mechanical impacts-electrical stimulation(RMI-ES)system utilizing a BaTiO_(3)/polydimethylsiloxane(PDMS)piezoelectric composite *** to conventional electrical stimulation devices,the fabricated piezoelectric composite film efficiently harvests energy from the pressure applied by the stimulation device and the tensile force occurring during natural rat *** results demonstrated that piezoelectric stimulation generated by the composite membrane expedited the cell cycle,promoting fibroblast ***,piezoelectric stimulation induced favorable changes in fibroblast gene expression,including increased expression of transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),collagen 1,collagen 3,vascular endothelial growth factor(VEGF),and alpha-smooth muscle actin(α-SMA),while reducing interleukin-6(IL-6)*** analysis revealed that piezoelectric stimulation may induce fibroblast migration,proliferation,and collagen expression by influencing PI3K/AKT serine/threonine kinase(AKT)*** confirmation through the addition of the PI3K inhibitor LY294002 validated that piezoelectric stimulation can regulate the repair process after skin injury through the ***,in vivo results demonstrated that the electric field at the wound site effectively promoted wound healing,reduced inflammation,and stimulated collagen deposition and ne

关键词： BaTiO3/polydimethylsiloxane(PDMS)nano-composite membrane piezoelectrical stimulation skin wound healing collagen deposition PI3K/AKT serine/threonine kinase(AKT)signaling pathway

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Electroacupuncture improves myocardial fibrosis in heart failure rats by attenuating ECM collagen deposition through modulation of TGF-β1/Smads signaling pathway

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Traditional Medicine Research 2024年第8期9卷 1-10页

作者： Wen-Hui Wang Qian-Lan Zeng Jiao-Jiao Zhang Hao-Sheng Wu Sheng-Bing Wu Mei-Qi Zhou College of Acupuncture and Massage Anhui University of Chinese MedicineHefei 230038China Anhui Province Key Laboratory of Meridian Viscera Correlationship Anhui University of Chinese MedicineHefei 230038China

Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01)

关键词： heart failure electroacupuncture heart meridian of Hand-Shaoyin collagen deposition TGF-β1/Smads signaling pathway myocardial fibrosis

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Acupotomy ameliorates knee osteoarthritis-related collagen deposition and fibrosis in rabbit skeletal muscle through the TGF-β/Smad pathway

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Journal of Traditional Chinese Medical Sciences 2024年第3期11卷 376-385页

作者： Hu T. Khavaza E. Liang C. Xing L. Chen X. Xu Y. Ma W. Mokhtari F. Lu J. Guo C. School of Acupuncture Moxibustion and Tuina Beijing University of Chinese Medicine Beijing 102488 China These authors have contributed equally to this work. Department of Traditional Chinese Medicine Peking University People's Hospital Beijing 100044 China The Fifth College of Clinical Medicine Guangzhou University of Traditional Chinese Medicine Guangzhou 511436 China Department of Acupuncture and Rehabilitation Guangdong Second Hospital of Traditional Chinese Medicine Guangzhou 510260 China Traditional Clinic Shahid Beheshti Medical University Tehran *** Iran Department of Traditional Chinese Medicine Shenzhen Hospital of Southern Medical University Shenzhen 510515 China

Objective: To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis (KOA) model. Methods: Rabbits (n = 18) were randomly divided into control, KOA, and KOA + acupotomy (Apo) groups (n = 6). The rabbits in the KOA and Apo groups were modeled using the modified Videman's method for 6 weeks. After modeling, the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa tendons around the knee. The behavior of all animals was recorded, rectus femoris tissue was obtained, and histomorphological changes were observed using Masson staining and transmission electron microscopy. The expression of transforming growth factor-β1 (TGF-β1), Smad 3, Smad 7, fibrillar collagen types I (Col-I) and III (Col-III) was detected using Western blot and real-time polymerase chain reaction (RT-PCR). Results: Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris, compared with the KOA group (P = .034). Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA, thus preventing skeletal muscle fibrosis. Western blot and RT-PCR analysis revealed that in the Apo group, Col-I, and Col-III protein levels were significantly lower than those in the KOA group (both P collagen deposition through the TGF-β/Smad pathway in the skeletal musc

关键词： Acupotomy collagen deposition Fibrosis Knee osteoarthritis Skeletal muscle

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Connective Tissue Growth Factor Expression in Human Bronchial Epithelial Cells

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 53-57页

作者： AmritaDOSANJH Department of Molecular and Experimental Medicine the Scripps Research Institute USA La Jolla 92122

Connective tissue growth factor (CTGF) is a cysteine-rich protein that promotes extracellularmatrix *** is selectively induced by transforming growth factor β and des-Arg kallidin in lungfibroblasts and increases steady-state mRNA levels of α type I collagen,5α-integrin and fibronectin *** epithelial cells have been proposed to functionally interact with lung *** investigated if bronchial epithelial cells are able to synthesize *** bronchial epithelialcells were grown to subconfluence in standard growth *** cells grown in small airwaygrowth media were harvested following starvation for up to 24 *** of CTGF transcripts wasmeasured by *** was also completed using a commercially available *** cells expressed readily detectable CTGF *** of these cells resulted in a quantitativedecline of CTGF *** sequencing of the PCR product identified human ***-tochemistry confirmed intracellular CTGF in the cells and none in negative control *** conclude thatbronchial epithelial cells could be a novel source of *** epithelial cell-derived CTGF could thusdirectly influence the deposition of collagen in certain fibrotic lung diseases.

关键词： bronchial epithelial cell connective tissue growth factor collagen deposition remodeling

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A novel chronic nerve compression model in the rat

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Neural Regeneration Research 2018年第8期13卷 1477-1485页

作者： Zhen-Yu Liu Zhen-Bing Chen Jiang-Hai Chen Department of Hand Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology

Current animal models of chronic peripheral nerve compression are mainly silicone tube models. However, the cross section of the rat sciatic nerve is not a perfect circle, and there are differences in the diameter of the sciatic nerve due to individual differences. The use of a silicone tube with a uniform internal diameter may not provide a reliable and consistent model. We have established a chronic sciatic nerve compression model that can induce demyelination of the sciatic nerve and lead to atrophy of skeletal muscle. In 3-week-old pups and adult rats, the sciatic nerve of the right hind limb was exposed, and a piece of surgical latex glove was gently placed under the nerve. N-butyl-cyanoacrylate was then placed over the nerve, and after it had set, another piece of glove latex was placed on top of the target area and allowed to adhere to the first piece to form a sandwich-like complex. Thus, a chronic sciatic nerve compression model was produced. Control pups with latex or N-butyl-cyanoacrylate were also prepared. Functional changes to nerves were assessed using the hot plate test and electromyography. Immunofluorescence and electron microscopy analyses of the nerves were performed to quantify the degree of neuropathological change. Masson staining was conducted to assess the degree of fibrosis in the gastrocnemius and intrinsic paw muscles. The pup group rats subjected to nerve compression displayed thermal hypoesthesia and a gradual decrease in nerve conduction velocity at 2 weeks after surgery. Neuropathological studies demonstrated that the model caused nerve demyelination and axonal irregularities and triggered collagen deposition in the epineurium and perineurium of the affected nerve at 8 weeks after surgery. The degree of fibrosis in the gastrocnemius and intrinsic paw muscles was significantly increased at 20 weeks after surgery. In conclusion, our novel model can reproduce the functional and histological changes of chronic nerve compression injury tha

关键词： nerve regeneration chronic nerve compression carpal tunnel syndrome nerve conduction velocity N-butyl-cyanoacrylate hypoesthesia demyelination remyelination intrinsic muscles collagen deposition axonal irregularity neural regeneration

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Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold

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World Journal of Stem Cells 2021年第1期13卷 115-127页

作者： Bing-Yu Zhang Pu Xu Qing Luo Guan-Bin Song Department of College of Bioinformatics Chongqing University of Posts and TelecommunicationsChongqing 400065China Department of College of Bioengineering Chongqing UniversityChongqing 400030China

BACKGROUND collagen is one of the most commonly used natural biomaterials for tendon tissue *** of the possible practical ways to further enhance tendon repair is to combine a porous collagen sponge scaffold with a suitable growth factor or cytokine that has an inherent ability to promote the recruitment,proliferation,and tenogenic differentiation of ***,there is an incomplete understanding of which growth factors are sufficient and optimal for the tenogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs)in a collagen sponge-based 3D culture *** To identify one or more ideal growth factors that benefit the proliferation and tenogenic differentiation of rat BMSCs in a porous collagen sponge *** We constructed a 3D culture system based on a type I collagen sponge *** surface topography of the collagen sponge scaffold was observed by scanning electron *** BMSCs were isolated from Sprague-Dawley *** survival on the surfaces of the scaffolds with different growth factors was assessed by live/dead assay and CCK-8 *** mRNA and protein expression levels were confirmed by quantitative real-time polymerase chain reaction and Western blot,*** deposited collagen was assessed by Sirius Red *** Transforming growth factorβ1(TGF-β1)showed great promise in the tenogenic differentiation of BMSCs compared to growth differentiation factor 7(GDF-7)and insulin-like growth factor 1(IGF-1)in both the 2D and 3D cultures,and the 3D culture enhanced the differentiation of BMSCs into tenocytes well beyond the level of induction in the 2D culture after TGF-β1 *** the 2D culture,the proliferation of the BMSCs showed no significant changes compared to the control group after TGF-β1,IGF-1,or GDF-7 ***,TGF-β1 and GDF-7 could increase the cell proliferation in the 3D ***,we also found more dead cells in the BMSC-collagen sponge constructs that

关键词： Bone marrow mesenchymal stem cells collagen sponge Transforming growth factorβ1 Tenogenic differentiation Proliferation collagen deposition

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Inhibition of TGF-β1 by eNOS gene transfer provides cardiac protection after myocardial infarction

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The Journal of Biomedical Research 2010年第2期24卷 145-152页

作者： Wei Qin Xin Chen Peisheng Liu Department of Cardiothoracic Surgery Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210006 China

Objective： Endothelial nitric oxide synthase （eNOS） and nitric oxide （NO） have been implicated in protection against myocardial ischemia injury. This study was designed to explore a new method of therapy for myocardial injury by eNOS gene transfection. Methods： A rat model of myocardial infarction （MI） was established by left anterior descending （LAD） coronary artery ligation, eNOS gene in an adenovirus vector was delivered locally into the rat heart and hemodynamic parameters were examined after 3 weeks, Matrix metalloproteinase-2 and 9 （MMP-2, MMP-9） mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR）, and the protein levels of eNOS, caspase-3, and transforming grouth factor 131 （TGF-131） were determined by western blot assay. Results： eNOS gene transfer significantly reduced cardiomyocyte apoptosis and improved cardiac function. In addition, eNOS significantly reduced the mRNA levels of MMP-2 and MMP-9. In the eNOS gene transfected group, the activation of caspase-3 and TGF-β1 were decreased. However, the protection was reversed by administration of the NOS inhibitor, N（o））-nitro-l-arginine methyl ester （L-NAME）. Conclusion： These results demonstrate that the eNOS provides cardiac protection after myocardial infarction injury through inhibition of cardiac apoptosis and collagen deposition, and suppression of TGF-β1.

关键词： endothelial nitric oxide synthase gene myocardial infarction cardiac function cardiomyocyte apoptosis collagen deposition transforming growth factor-β1

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P311 promotes typeⅡtransforming growth factor-βreceptor mediated fibroblast activation and granulation tissue formation in wound healing

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Burns & Trauma 2022年第1期10卷 175-190页

作者： Jue Wang Ruoyu Shang Jiacai Yang Zhihui Liu Yunxia Chen Cheng Chen Wenxia Zheng Yuanyang Tang Xiaorong Zhang Xiaohong Hu Yong Huang Han-Ming Shen Gaoxing Luo Weifeng He State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Key Laboratory for Disease Proteomics Chongqing 400038China Department of Technical Support Chengdu Zhijing Technology Co.LtdChengdu 610041China Academy of Biological Engineering Chongqing UniversityChongqing 400038China Faculty of Health Sciences University of MacaoMacaoChina

Background:P311,a highly conserved 8 kDa intracellular protein,has recently been reported to play an important role in aggravating hypertrophic scaring by promoting the differentiation and secretion of ***,how P311 regulates the differentiation and function of fibroblasts to affect granulation tissue formation remains *** this work,we studied the underlying mechanisms via which P311 affects fibroblasts and promotes acute skin wound ***:To explore the role of P311,both in vitro and in vivo wound-healing models were ***-thickness skin excisional wounds were made in wild-type and P311−/−C57 adult *** healing rate,re-epithelialization,granulation tissue formation and collagen deposition were measured at days 3,6 and 9 after skin *** biological phenotypes of fibroblasts,the expression of target proteins and relevant signaling pathways were examined both in vitro and in ***:P311 could promote the proliferation and differentiation of fibroblasts,enhance the ability of myofibroblasts to secrete extracellular matrix and promote cell contraction,and then facilitate the formation of granulation tissue and eventually accelerate skin wound ***,we discovered that P311 acts via up-regulating the expression of type II transforming growth factor-βreceptor(TGF-βRII)in fibroblasts and promoting the activation of the TGF-βRII-Smad signaling ***,the mammalian target of rapamycin signaling pathway is closely implicated in the regulation of the TGF-βRII-Smad pathway in fibroblasts mediated by ***:P311 plays a critical role in activation of the TGF-βRII-Smad pathway to promote fibroblast proliferation and differentiation as well as granulation tissue formation in the process of skin wound repair.

关键词： P311 Wound healing Fibroblasts TGF-βRII-Smad pathway Re-epithelialization Granulation tissue formation collagen deposition

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