Connective Tissue Growth Factor Expression in Human Bronchial Epithelial Cells

作     者：AmritaDOSANJH 

作者机构：Department of Molecular and Experimental Medicine the Scripps Research Institute USA La Jolla 92122 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2006年第38卷第1期

页      面：53-57页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0703[理学-化学] 10[医学] 

基　　金：AcknowledgementsTheauthorwouldliketothankSandraCHAMBERSandCarolPEEBLESfortheirexperttechnicalassistance andDr.ZURAWforreviewofthemanuscript 

主　　题：bronchial epithelial cell connective tissue growth factor collagen deposition remodeling 

摘      要：Connective tissue growth factor (CTGF) is a cysteine-rich protein that promotes extracellularmatrix *** is selectively induced by transforming growth factor β and des-Arg kallidin in lungfibroblasts and increases steady-state mRNA levels of α type I collagen,5α-integrin and fibronectin *** epithelial cells have been proposed to functionally interact with lung *** investigated if bronchial epithelial cells are able to synthesize *** bronchial epithelialcells were grown to subconfluence in standard growth *** cells grown in small airwaygrowth media were harvested following starvation for up to 24 *** of CTGF transcripts wasmeasured by *** was also completed using a commercially available *** cells expressed readily detectable CTGF *** of these cells resulted in a quantitativedecline of CTGF *** sequencing of the PCR product identified human ***-tochemistry confirmed intracellular CTGF in the cells and none in negative control *** conclude thatbronchial epithelial cells could be a novel source of *** epithelial cell-derived CTGF could thusdirectly influence the deposition of collagen in certain fibrotic lung diseases.