Acupotomy ameliorates knee osteoarthritis-related collagen deposition and fibrosis in rabbit skeletal muscle through the TGF-β/Smad pathway
作者机构：School of Acupuncture Moxibustion and Tuina Beijing University of Chinese Medicine Beijing 102488 China These authors have contributed equally to this work. Department of Traditional Chinese Medicine Peking University People's Hospital Beijing 100044 China The Fifth College of Clinical Medicine Guangzhou University of Traditional Chinese Medicine Guangzhou 511436 China Department of Acupuncture and Rehabilitation Guangdong Second Hospital of Traditional Chinese Medicine Guangzhou 510260 China Traditional Clinic Shahid Beheshti Medical University Tehran *** Iran Department of Traditional Chinese Medicine Shenzhen Hospital of Southern Medical University Shenzhen 510515 China
出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)
年 卷 期：2024年第11卷第3期
页 面：376-385页
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：National Natural Science Foundation of China, NSFC, (82074523, 82004448) National Natural Science Foundation of China, NSFC
主 题：Acupotomy Collagen deposition Fibrosis Knee osteoarthritis Skeletal muscle
摘 要：Objective: To investigate the effects of acupotomy on skeletal muscle fibrosis and collagen deposition in a rabbit knee osteoarthritis (KOA) model. Methods: Rabbits (n = 18) were randomly divided into control, KOA, and KOA + acupotomy (Apo) groups (n = 6). The rabbits in the KOA and Apo groups were modeled using the modified Videman s method for 6 weeks. After modeling, the Apo group was subjected to acupotomy once a week for 3 weeks on the vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa tendons around the knee. The behavior of all animals was recorded, rectus femoris tissue was obtained, and histomorphological changes were observed using Masson staining and transmission electron microscopy. The expression of transforming growth factor-β1 (TGF-β1), Smad 3, Smad 7, fibrillar collagen types I (Col-I) and III (Col-III) was detected using Western blot and real-time polymerase chain reaction (RT-PCR). Results: Histological analysis revealed that acupotomy improved the microstructure and reduced the collagen volume fraction of rectus femoris, compared with the KOA group (P = .034). Acupotomy inhibited abnormal collagen deposition by modulating the expression of fibrosis-related proteins and mRNA, thus preventing skeletal muscle fibrosis. Western blot and RT-PCR analysis revealed that in the Apo group, Col-I, and Col-III protein levels were significantly lower than those in the KOA group (both P .01), same as Col-I and Col-III mRNA levels (P = .0031; P = .0046). Compared with the KOA group, the protein levels of TGF-β1 and Smad 3 were significantly reduced (both P .01), as were the mRNA levels of TGF-β1 and Smad 3 (P = .0007; P = .0011). Conversely, the levels of protein and mRNA of Smad 7 were significantly higher than that in the KOA group (P .01; P = .0271). Conclusion: Acupotomy could alleviate skeletal muscle fibrosis and delay KOA progress by inhibiting collagen deposition through the TGF-β/Smad pathway in the skeletal musc
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