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cathepsin b protease is required for metamorphism in silkworm, bombyx moil

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Insect Science 2008年第3期15卷 201-208页

作者： Gen-Hong Wang Chun Liu Qing-You Xia Xing-Fu Zha Jie Chen Liang Jiang Key Sericultural Laboratory of the Agricultural Ministry College of Biotechnology Southwest University Chongqing China

cathepsin b belongs to lysosomal cysteine protease of the papain family. Temporal and spatial expression analysis of cathepsin b of bombyx mori （bmCtb） was carried out based on Expression Sequence Tags （ESTs） data, oligonucleotide microarray, reverse transcription polymerase chain reaction （RT-PCR） and quantitative real-time PCR. Expression of bmCtb was observed in all of the tissues and stages. Among the 10 tested tissues, the fat body and posterior silk gland are the two most enriched tissues with bmCtb. During bombyx development, there was an expression fastigium of bmCtb during metamorphosis. RNA interference was used to suppress the expression of cathepsin b during metamorphosis. Significant developmental defective phenotypes were obtained in the RNAi treated group. The dramatically reduced expression of bmCtb was con^rmed by Northern blot and quantitative real-time PCR. These evidences strongly suggest cathepsin b proteinase was predominantly involved in the metabolism process of fat body and the posterior silk gland and was critical for metamorphism and development of silkworm, bombyx mori.

关键词： cathepsin b RNA interference quantitative real-time PCR bombyx mori

cathepsin b-responsive nanodrug delivery systems for precise diagnosis and targeted therapy of malignant tumors

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Chinese Chemical Letters 2020年第12期31卷 3027-3040页

作者： Yanan Li Ting Mei Shupeng Han Tao Han Yongbing Sun Hui Zhang Feifei An Department of Radiology First Hospital of Shanxi Medical UniversityTaiyuan 030001China College of Medical Imaging Shanxi Medical UniversityTaiyuan 030001China Institute of Medical Engineering Department of Biophysicschool of Basic Medical ScienceHeatih Science Center Xi'an Jiaotong UniverstyXi'an 710061China College of Chemistry and Life Science Institute of Functional MoleculesChengdu Normal UniversityChengdu 611130China Division of Pharmaceutics National Pharmaceutical Engineering Center for Solid Preparation in Chinese Hierbal MedicimeJiamgpri Unverity of Truditional Chinese MedicinveNanchang 330006China

The tumor microenvironment(TME)significantly influences cancer evolution and therapeutic *** biofunctional molecules to the TME has long been appreciated as a means of raising local drug concentrations and reducing systemic *** booming nanotechnology field has realized the importance of cathepsin b to derive a variety of intelligent enzyme-responsive nanosized drug delivery systems(nanoDDS)to improve treatment responses and clinical *** this tutorial review,after introducing the molecular structure and physiological/pathological functions of cathepsin b,the outstanding achievements of cathepsin b-responsive nanoplatforms in the precise diagnosis,targeted therapy,and synergistic theranostics of malignant tumors are systematically ***,the challenges of enzyme-substrate incompatibility,low diagnostic sensitivity,mass production and biocompatibility of multifunctional nanoDDS are considered in order to successfully promote them to clinical applications.

关键词： Tumor microenvironment cathepsin b Theranostics Precise diagnosis Targeted therapy

cathepsin b EXPRESSION AND ITS RELATIONSHIP WITH MICROVESSEL DENSITY AND bIOLOGICAL bEHAVIOUR OF COLORECTAL CARCINOMA

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Chinese Journal of Cancer Research 2002年第4期14卷 293-296页

作者： WANG Ya-lan王娅兰 LIN Xiao林晓 Department of Pathology Chongqing University of Medical Sciences Chongqing 400016 Department of Pathology Chongqing University of Medical Sciences Chongqing 400016

Objective: To investigate cathepsin b(Cb) expression in colorectal carcinoma and its relationship with microvessel density (MVD) and biological behavior. Methods: Cb and MVD were detected by immunohistochemistry in 47 cases of colorectal carcinoma. Results: The expression of Cb in mucinous colorectal carcinoma was significantly higher than that in no-mucinous colorectal carcinoma. There was significant difference (Pb was stronger than that in group with negative Cb. There was also significant difference (Pb expression has correlation with MVD, invasion and metastasis in colorectal carcinoma, especially in mucinous colorectal carcinoma.

关键词： Colorectal carcinoma cathepsin b Invasion Metastasis Angiogenesis

Catechu Inhibiting cathepsin b Activity and Motility of HT1080 Cells

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Chemical Research in Chinese Universities 2011年第2期27卷 269-272页

作者： YUAN Hao YANG Jin-gang WANG Zhong-yuan ZHAO Shu-hua YU Da-hai FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering Ministry of Education College of Life Science Jilin University Changchun 130012 P. R. China Department of Traditional Chinese Medicine China-Japan Union Hospital of Jilin University Changchun 130041 P R. China

We reported that Catechu extract has a significant inhibitory effect on cathepsin b activity. The IC50 value for the Catechu extract against cathepsin b was 7.6 μg/mL. In addition, we showed that HT1080 human fibrosarcoma cells express cathepsin b and Catechu modulate the invasion and motility of these cells. These data may provide molecular mechanisms for the therapeutic effects of Catechu.

关键词： cathepsin b Traditional Chinese medicine Catechu Tumor Cell invasion Cell motility

Icariin plus curcumol enhances autophagy through the mTOR pathway and promotes cathepsin b-mediated pyroptosis of prostate cancer cells

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Asian Pacific Journal of Tropical biomedicine 2024年第2期14卷 55-64页

作者： Xu-Yun Wang Wen-Jing Xu bo-Nan Li Tian-Song Sun Wen Sheng Department of Andrology Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing100010China Department of Dermatology The First Affiliated Hospital of Hunan University of Chinese MedicineChangsha410021China School of Integrated Chinese and Western Medicine Hunan University of Chinese MedicineChangsha410208China Andrology Laboratory Hunan University of Chinese MedicineChangsha410208China School of Rehabilitation Medicine and Health Care Hunan University of MedicineHuaihua418000China School of Traditional Chinese Medicine Hunan University of MedicineHuaihua418000China

Objective:To examine the effect of icariin plus curcumol on prostate cancer cells PC3 and elucidate the underlying ***:We employed the Cell Counting Kit 8 assay and colony formation assay to assess cell viability and *** expression was analyzed using monodansylcadaverine *** and Western blot analyses were used to evaluate protein expressions related to autophagy,pyroptosis,and the mTOR *** damage was examined using the lactate dehydrogenase ***,cathepsin b and NLRP3 were detected by ***:Icariin plus curcumol led to a decrease in PC3 cell proliferation and an enhancement of *** levels of LC3-Ⅱ/LC3-Ⅰand beclin-1 were increased,while the levels of p62 and mTOR were decreased after treatment with icariin plus *** changes were reversed upon overexpression of ***,3-methyladenine resulted in a decrease in inflammatory cytokines,pyroptosis-related protein levels,and lactate dehydrogenase concentration,compared to the icariin plus curcumol *** cathepsin b reversed the regulatory effects of icariin plus ***:Icariin plus curcumol demonstrates great potential as a therapeutic agent for castration-resistant prostate cancer by enhancing autophagy via the mTOR pathway and promoting pyroptosis mediated by cathepsin *** findings provide valuable insights into the molecular mechanisms underlying the therapeutic potential of icariin and curcumol for prostate cancer treatment.

关键词： Icariin Curcumol Autophagy mTOR cathepsin b Pyroptosis Prostate cancer

Relation of Cystatin C and cathepsin b Expression to the Pathological Grade and Invasion of Human Gliomas

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Chinese Journal of Clinical Oncology 2007年第5期4卷 303-306页

作者： Kai Fan Yanli Zhang Dawei Song Yuchun Zhang Jianmei Ma Department of Anatomy Dalian Medical University Dalian 116027 China Department of Neurosurgery the First Hospital of JinzhouDalian 116031 China.

ObJECTIVE To explore the relation of cystatin C and cathepsin b expression to the pathological grade and invasion of human gliomas. METHODS A immunohistochemical method was used to detect the expression of cystatin C and cathepsin b in 57 glioma samples. RESULTS The expression of cystatin C in high-grade （Grade Ⅲ-Ⅳ ）gliomas was significantly weaker than that in low-grade（Grade Ⅰ-Ⅱ, P=0.0001）. On the other hand, the expression of cathepsin b in high-grade gliomas was significantly stronger than that in low-grade （P=0.0001）. Cystatin C expression correlated inversely with cathepsin b expression in gliomas （P=0.01）. CONCLUSION Cystatin C and cathepsin b expression is related to the pathological grade and invasion of gliomas. Combining detection of cystatin C and cathepsin b expressions might provide significant information for clinical assessment of maglignant phenotypes and invasion of gliomas.

关键词： gliomas cathepsin b cystatin C.

Role of cathepsin b-mediated apoptosis in fulminant hepatic failure in mice

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World Journal of Gastroenterology 2009年第10期15卷 1231-1236页

作者： bing-Zhu Yan Wei Wang Li-Yan Chen Man-Ru bi Yan-Jie Lu bao-Xin Li bao-Shan Yang Department of Infectious Diseases the SecondClinical Hospital of Harbin Medical University Harbin 150086China Department of Pharmacology HarbinMedical University Harbin 150086 China

AIM: To investigate the pathogenic role of cathepsin b and the protective effect of a cathepsin b inhibitor (CA074Me) in fulminant hepatic failure in mice. METHODS: LPS/D-Gal N was injected into mice of the model group to induce fulminant hepatic failure; the protected group was administered CA-074me for 30 min before LPS/D-Gal N treatment; the normal group was given isochoric physiologic saline. Liver tissue histopathology was determined with HE at 2, 4, 6 and 8 h after Lps/D-Gal injection. Hepatocyte apoptosis was examined by TUNEL method. The expression of cathepsin b in liver tissues was investigated by immunohistochemistry, Western blot and RT-PCR. RESULTS: Compared with the normal group, massive typical hepatocyte apoptosis occurred in the model group; the number of apoptotic cells reached a maximum 6 h after injection. The apoptosis index (AI) in the protected group was clearly reduced (30.4 ± 2.8 vs 18.1 ± 2.0, P cathepsin b activity was markedly increased in drug-treated mice compared with the normal group (P bation with LPS/D-Gal N at selected time points resulted in a timedependent increase in cathepsin b activity, and reached a maximum by 8 h. The expression of cathepsin b was significantly decreased in the protected group (P cathepsin b plays an essential role in the pathogenesis of fulminant hepatic failure, and the cathepsin b inhibitor CA-074me can attenuate apoptosis and liver injury.

关键词： Fulminant hepatic failure Hepatocyteapoptosis cathepsin b CA-O74me

Changes of cathepsin b in human photoaging skin both in vivo and in vitro

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Chinese Medical Journal 2010年第5期123卷 527-531页

作者： LAI Wei ZHENG Yue YE Zhang-zhang SU Xiang-yang WAN Miao-jian GONG Zi-jian XIE Xiao-yuan LIU Wei Department of Dermatovenereology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China Department of Dermatology General Hospital of Air Force of the Chinese People's Liberation Army Beijing 100142 China

background cathepsin b plays an important role tumorigenesis： whether it plays a role in photoaged skin cathepsin b in skin photoaging in vivo and in vitro. n cell cycle, extracellular matrix changes and cutaneous remains unknown. This study aimed to investigate the role of Methods The expressions of cathepsin b were compared with immunohistochemical methods in solar exposed skin and solar protected skin of six healthy Chinese volunteers. The mRNA and protein expression of cathepsin b in ultraviolet light A （UVA） induced premature senescence fibroblasts in vitro were detected by real-time reverse transcription polymerase chain reaction （RT-PCR） and Western blotting technique. Results Decreased expression of cathepsin b was observed in photoaged skin compared with that of the solar protected skin. In the UVA induced, premature senescence fibroblasts, a lower expression of cathepsin b was detected by Western blotting and a decreased synthesis of cathepsin b mRNA in the same cells was revealed by real-time RT-PCR. Conclusions The results demonstrated a significant negative correlation between skin photoaging and cathepsin b in vitro and in vivo. We propose that cathepsin b, besides matrix metalloproteinases and antioxidant enzymes, is involved in the process of skin photoaging in that it contributes to extracellular matrix remodelling and is a dominant protease in cellular apoptosis and senescence.

关键词： cathepsin b ultraviolet light photoaging skin

Qushi Huayu Decoction (祛湿化瘀方) Inhibits Protein and Gene Expression of cathepsin b in HepG2 Cells Induced by Free Fatty Acids

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Chinese Journal of Integrative Medicine 2010年第6期16卷 518-524页

作者：冯琴成扬胡义扬张慧彭景华张宁 Institute of Liver Disease Shuguang HospitalShanghai University of Traditional Chinese Medicine

Objective： To study the experimental efficacy of Qushi Huayu Decoction （祛湿化瘀方,QHD） on protein and gene expression of cathepsin b （ctsb） in HepG2 cells induced by free fatty acids （FFAs）.Methods： The model of HepG2 steatosis and tumor necrosis factor-α （TNF-α） secretion was induced by long-chain ***2 cells were divided into 4 groups： control group （group C）,model group （group M）,low-dose QHD group （group L） and high-dose QHD group （group H ）.Long-chain FFAs were added to groups M,L and *** 10% blank-control serum was added to group C and M,while 5% and 10% QHD-containing sera were added to group L and H,*** levels of serum TNF-α and cellular triglyceride （TG） were *** p-Iκb and ctsb expression were detected using Western blot and *** expression and distribution of ctsb were observed by ***： After incubating with FFA for 24 h,TG deposition in HepG2,TNF-α content in cell supernatant,the protein expression of cellular ctsb and P-Iκb,as well as mRNA expression of ctsb increased markedly in group M compared with group C （P〈0.05,P〈0.01）.Compared with group M,TG deposition,the expression of cellular ctsb,P-Iκb and ctsb mRNA in groups L and H,as well as TNF-α content in group H,decreased significantly （P〈0.05）.Cell immunochemical fluorescence studies showed that ctsb was released from lysosomes and distributed in the cytoplasm extensively and diffusedly after being stimulated with *** this study,these above-mentioned changes were inhibited markedly in groups L and ***： QHD might have a direct inhibitory effect on the ctsb target in the FFA-ctsb-TNFα pathway of hepatic lipotoxicity.

关键词： Qushi Huayu Decoction hepatic lipotoxicity free fatty acid cathepsin b