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Agricultural Sciences 2020年第2期11卷 111-123页

作者： Karine Moche Paul-Alain Nana Liliane Tandzi Ngoune Champlain Djieto-Lordon Noé Woin Télesphore Sime-Ngando Laboratory of Entomology Institute of Agricultural Research for Development Yaoundé Cameroon Department of Oceanography and Limnology Institute of Fisheries and Aquatic Sciences University of Douala Douala Cameroon Laboratory Microorganisms: Genome and Environment (LMGE) Clermont Auvergne University Clermont-Ferrand France Department of Agriculture College of Basic and Apply Sciences University of Ghana Legon Ghana Laboratory of Zoology Faculty of Science University of Yaoundé Yaoundé Cameroon

The study compares the impact due to rice stem borers in two sites (Yaoundé and Ntui). It also shows the diversity of the African Rice Gall Midge (AfRGM) biotypes in southern Cameroon (Santchou, Ndop, Tonga, Ebolowa, Ba?gom, Yaoundé and Ntui). The New Rice for Africa (NERICA) varieties 3, 8, 9 and 13 sown in Ntui were less attacked than those sown in Yaoundé. At both sites, damages ranged from 0.78% to 2.7%. In terms of diversity, the main stem-borer species were O. oryzivora, Diopsis apicalis, D. longiconis and Chilo zacconius. Molecular analyses of Orseolia oryzivora larvae collected in the localities of Santchou, Ndop, Tonga, Ebolowa, Ba?gom and Yaoundé showed the existence of more than one O. oryzivora biotype in southern Cameroon’s rice basins.

关键词： biotype Larvae NERICA Orseolia sp PCR

Identification and characterization of a cytochrome P450 C YP6CX1 putatively associated with insecticide resistance in Bemisia tabaci

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Insect Science 2011年第5期18卷 484-494页

作者： Hua-Mei Zhuang Kuan-Fu Wang Lin Zheng Zu-Jian Wu Tadashi Miyata Gang Wu Key Laboratory of Biopesticide and Chemical Biology Ministry of Education Fujian Agriculture and Forestry University Fuzhou Fujian China Laboratory of Applied Entomology Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan

The novel full length of cytochrome P450 gene has been isolated in insecticideresistant （named CYP6CXlvl） and -susceptible （named CYP6CXlv2） Bemisia tabaci, which was identified as B biotype, in Shangjie, Fujian, China （Sj）. CYP6CX1 （1 940 bp contained a 1 557 bp open reading frame） included conserved domains common to CYP6 members, such as heme-binding motif PFGEGPRFCIA, putative ＂meander＂-binding sequence ETLR and PERF in helix-K, oxygen-binding motif AGLDPV and conserved sequence PEKFNP near the carboxyl end. There were four different replacements of amino acid residues between R and S B. tabaci （Thr300 Ala, Thr354Pro, Arg486His and Ile503Thr）, among which the substitution Ile503Thr was located in the substrate recognition sites region. The mRNA transcription level of CYP6CXlvl was 2.38-fold as high as that of CYP6CXlv2. The results indicated that the CFP6CX1 from the B biotype B. tabaci in Sj was one of the CYP6 members, and enhanced CYP6CX1 expression and substitute of amino acid residues might be involved in the resistance mechanisms in field B. tabaci.

关键词： Bemisia tabaci biotype cytochrome P450s monooxygenases insecticide resistance

Genetic mutations associated with chemical resistance in the cytochrome P450 genes of invasive and native Bemisia tabaci （Hemiptera： Aleyrodidae） populations in China

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Insect Science 2009年第3期16卷 237-245页

作者： Bao-Li Qiu Li Liu Xiao-Xi Li Vartika Mathur Zhen-Qiang Qin Shun-Xiang Ren Department of Entomology South China Agricultural University Guangzhou China Department of Zoology Sri Venkateswara CollegeUniversity of Delhi Delhi 110021 India

Bemisia tabaci （Gennadius） is a species complex, and its two most damaging biotypes B and Q are globally distributed pests. Despite increasing biological and economic impacts, little is known about the evolutionary mechanisms that favor their competition with native populations. Here, we investigated the genetic mutations in the P450 gene of the invasive B, Q biotypes and the native Cv population. Four mutations associated with chemical resistance, Pro-Leu, Ala-Ser, Ser-Phe and Trp-Leu, were found in the cytochrome P450 CYP6C and CYP9F genes of the B and Q biotypes. Bioassay results also revealed that both the B and Q biotypes have about 12-47 times more resistance to acephate, beta- cypermethrin, methomyl, and 5-7 times more resistance to imidacloprid insecticide than Cv population. Our results provide a molecular approach for better understanding and monitoring the pesticide resistances of invasive and native B. tabaei populations in China.

关键词： Bemisia tabaci biotype cytochrome P450 gene genetic mutation pesticide resistance

Fitness traits and underlying genetic variation related to host plant specialization in the aphid Sitobion avenae

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Insect Science 2014年第3期21卷 352-362页

作者： Su-Xia Gao De-Guang Liu Hui Chen Xiu-Xiang Meng State Key Laboratory of Crop Stress Biology for Arid Areas (Northwest A&F University) Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of Agriculture College of Forestry Northwest A&F University Yangling Shaanxi College of Life and Environmental Sciences Minzu University of China Beijing China

Sitobion avenae （E） is an important cereal pest worldwide that can survive on various plants in the Poaceae, but divergent selection on different host plants shouldpromote the evolution of specialized genotypes or host races. In order to evaluate their resource use strategies, clones of S. avenae were collected from oat and barley. Host-transfer experiments for these clones were conducted in the laboratory to compare their fitness traits. Our results demonstrated that barley clones had significantly lower fecundityand tended to have longer developmental times when transferred from barley to oat. However, oat clones developed faster after they were transferred to barley. Clones fromoat and barley had diverged to a certain extent in terms of fecundity and developmental time of the nymphs. The separation of barley clones and oat clones of S. avenae was alsoevident in a principal component analysis. Barley clones tended to have higher broad-sense heritabilities for fitness traits than oat clones, indicating the genetic basis of differentiationbetween them. Barley clones showed significantly higher extent of specialization compared to oat clones from two measures of specialization （i.e., Xsp and Ysp）. Therefore, barleyclones were specialized to a certain extent, but oat clones appeared to be generalized. The fitness of S. avenae clones tended to increase with higher extent of specialization. Theevolution toward ecological specialization in S. avenae clones, as well as the underlying genetic basis, was discussed.

关键词： biotype ecological specialization population divergence sympatric speciation wheat aphid

Sequence Analysis of mtDNA CO/Gene and Molecular Phylogeny of Different Geographical Populations of Bemisia tabaci (Gennadius)

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Agricultural Sciences in China 2005年第7期4卷 533-541页

作者： CHUDong ZHANGYou-jun CONGBin XUBao-yun WUQing-jun ZHUGuo-ren InstituteofVegetablesandFlowers ChineseAcademyofAgriculturalSciencesBeijing100081P.R.China InstituteofVegetablesandFlowers ChineseAcademyofAgriculturalSciencesBeijing100081P.R.China／／CollegeofPlantProtectionShenyangAgriculturalUniversityShenyang110161P.R.China CollegeofPlantProtection ShenyangAgriculturalUniversityShenyang110161P.R.China

Bemisia tabaci(Gennadius) is a serious pest in many cropping systems worldwide and occurs in different biotypes. ThemtDNA COI gene of the 12 Bemisia tabaci (Gennadius) populations from different regions and countries were *** on mtDNA COI sequences, their biotypes were characterized and phylogenetic relationships among thesepopulations were established with the method of UPGMA. The results indicated the genetic similarity between thosepopulations from Beijing, Zhengzhou, Zaozhuang, Nanjing, Shanghai, Haikou, and the B-biotype populations fromCalifornia, Texas, Arizona reached 99.8-100%, which meant the nation-wide infested populations of *** in China inrecent years were B-biotypes. Another population collected from Kunming of Yunnan Province showed very high similaritywith Q-biotype *** from Spain and Morocco, which meant the Kunming population was Q-biotype. This is the firstreport on the invasion of Q-biotype into China.

关键词： Bemisia tabaci (Gennadius) mtDNA COI biotype Phylogenetic tree

不同地理种群褐飞虱的致害性变异和为害特征(英文)

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Insect Science 1999年第2期6卷 146-154页

作者：吕仲贤俞晓平巫国瑞陶林勇陈建明郑许松杭州310021 浙江省农业科学院植物保护研究所

本文对采自广西、云南和浙江的不同地理种群的褐飞虱致害性变异和为害特性进行了比较研究。致害性测定结果表明，１９８９年后浙江种群褐飞虱仍表现为生物型２的致害特性；而广西种群在１９９１年后不仅能致害含ＢＰｈ１基因的ＩＲ２６... 详细信息

本文对采自广西、云南和浙江的不同地理种群的褐飞虱致害性变异和为害特性进行了比较研究。致害性测定结果表明，１９８９年后浙江种群褐飞虱仍表现为生物型２的致害特性；而广西种群在１９９１年后不仅能致害含ＢＰｈ１基因的ＩＲ２６和ＭＵｄｇｏ而且还能致害含ｂｐｈ２基因的ＡＳＤ７，１９９７年广西和云南种群均能致害抗虫品种ＩＲ２６、Ｍｕｄｇｏ、ＡＳＤ７和Ｒ．Ｈ，表明这两个种群产生了不同与生物型１、２和３的致害性更强的新的生物型。自由选择试验结果表明，浙江种群的若虫和成虫对不同抗虫品种的取食选择性有明显的差异，成虫嗜食ＩＲ２６品种而若虫对不同品种的选择性无显著差别。云南种群若虫嗜食ＡＳＤ７，而成虫则嗜食ＴＮ１。室内试验表明，浙江种群的若虫历期最长，而在广西种群中取食ＡＳＤ７的若虫历期明显比取食ＴＮ１的长。取食ＩＲ２６的浙江种群的若虫存活率比取食ＴＮ１和ＡＳＤ７的高，而在抗虫品种ＡＳＤ７和ＩＲ２６上云南种群的若虫存活率明显高于浙江和广西种群。研究表明不同地理种群的褐飞虱的致害性与其为害特性并不完全一致。

关键词： brown planthopper geographic population virulence change damage characteristics biotype

Comparative proteomics studies on brown planthopper responding to host rice resistance

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Comparative proteomics studies on brown planthopper respondi...

Nucleic Acid Determination and Risk Assessment of Brucella in Milk Products

2016年全国植物生物学大会

作者： Kaikai Zeng Siqi Wang Juanjuan Zhang Zhifan Yang Hubei Collaborative Innovation Center for Green Transformation of Bio-Resource College of Life SciencesHubei University

The brown planthopper(BPH,Nilaparvata lugens) is one of the most destructive pests of rice. Comparative proteomics analyses of BPH will help to find new measures to control BPH. In this work,comparative proteomics analyses of the fourth instar nymphs of biotype I and II which infested the susceptible rice TN1 and the resistant rice Mudgo,were carried out by isobaric tag-based methodology for relative peptide quantification(iTRAQ) technique. The results revealed that 7385 protein fragments were detected,and 696 fragments were quantified. Total 201 proteins showed obviously different expression levels. These proteins were expressed differently between the two types of BPH,or the same type BPH feeding on the two rice varieties with different resistant levels. 27 proteins were selected to investigate the expression level of the corresponding genes by fluorescent quantitative real-time PCR. The results revealed that most of the genes have the same expression trends with their coding proteins. It is noteworthy that three genes(vitellogenin,fatty acid synthase,protein kinase) were expressed at an extremely different pattern,and probably involved in regulating biotype formation and/or the acute stress reaction. This study will help in intensifying the knowledge of the interaction mechanism of BPH and rice,including: (1) during the acute stress reaction of BPH to host rice resistance,the variation laws of the midgut proteomics,which will indirectly provide some important information about the resistance mechanism of rice;(2) the discrepancy between different biotype BPH midgut proteomics,which will provide some important information for division of biotype and elucidating the molecular mechanism of the formation of a special biotype.

关键词： brown planthopper(Nilaparvata lugens Hemiptera Delphacidae) biotype host rice resistance isobaric tag-based methodology for relative peptide quantification(iTRAQ) comparative proteomics

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Animal Husbandry and Feed Science 2016年第2期8卷 82-84页

作者： Bai Bi Fang Ying Lin Lizhong Xu Chunzhi Yang Feng Chen Hong Peng Yi Yuan Cuixia Guiyang Animal Disease Prevention and Control Center Guiyang 550081 China Guiyang Animal Health Supervision Institute Guiyang 550081 China

Brucellosis is a worldwide zoonosis caused by Brucella, which poses great threat to human health. Totally 234 milk samples from a scale dairy farm were amplified by nested PCR, and the pathogens were further separated. The results showed that a DNA band of 419 bp was amplified from 18 out ot＂234 milk samples. Among 18 positive milk samples, nine samples amplified the DNA band of 535 bp, which were B. su/s ; one sample amplified the DNA bands of 535 bp and 285 bp, which was the mixture of B. su/s and B. bov/s. The results laid a foundation for prevention and control of brucellosis.

关键词： Brucella PCR biotype Risk assessment

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霍乱浅谈

旅行医学科学 1996年第4期2卷 191-193页

作者：魏承毓北京医科大学

尽管WHO一再强调当一个国家或地区宣布发生霍乱时并不应影响该国或该地区的旅游与经贸往来,但由于历史和现实的种种影响,人们却依然担心和惧怕这个曾给人类健康和生命造成极大危害急性肠道传染病。究竟霍乱是一种什么样的疾病:它的流行... 详细信息

尽管WHO一再强调当一个国家或地区宣布发生霍乱时并不应影响该国或该地区的旅游与经贸往来,但由于历史和现实的种种影响,人们却依然担心和惧怕这个曾给人类健康和生命造成极大危害急性肠道传染病。究竟霍乱是一种什么样的疾病:它的流行近况如何?人们应怎样去预防并完全战胜它?这里简介如下。

关键词： cholera clinical symptoms biotype serological type seventh pandemic prevention and control measures

Effect of Different Temperature and Humidity on Bemisia tabaci

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Journal of Botanical Research 2020年第2期2卷 21-24页

作者： Shunxiao Liu Hao Yu Vlasenko V.A Sumy National Agrarian University SumyG.Kondratyeva St.160Ukraine Henan Institute of Science and Technology Xinxiang 453003China

Bemisia tabaci has many biotypes,and has become one of the main pests on vegetables,flowers,cotton and other crops,seriously influencing the safe production of *** has a great influence on the survival,development,reproduction and behavior of Bemisia tabaci,which can affect the change of body temperature and metabolism of *** needs certain water to maintain normal life activities,such as digestion,nutrition transportation,and temperature *** is a suitable humidity range for the growth and development of *** in different development *** humidity or drought have a great impact on the growth and development of ***,especially on its survival or *** the same time,the environmental temperature and humidity are the important factors that influence the geographical distribution and population change of ***.

关键词： Bemisia tabaci biotype Temperature Humidity Population