Comparative proteomics studies on brown planthopper responding to host rice resistance
作者单位：Hubei Collaborative Innovation Center for Green Transformation of Bio-ResourceCollege of Life SciencesHubei University
会议名称：《2016年全国植物生物学大会》
会议日期：2016年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：brown planthopper(Nilaparvata lugens Hemiptera Delphacidae) biotype host rice resistance isobaric tag-based methodology for relative peptide quantification(iTRAQ) comparative proteomics
摘 要：The brown planthopper(BPH,Nilaparvata lugens) is one of the most destructive pests of rice. Comparative proteomics analyses of BPH will help to find new measures to control BPH. In this work,comparative proteomics analyses of the fourth instar nymphs of biotype I and II which infested the susceptible rice TN1 and the resistant rice Mudgo,were carried out by isobaric tag-based methodology for relative peptide quantification(iTRAQ) technique. The results revealed that 7385 protein fragments were detected,and 696 fragments were quantified. Total 201 proteins showed obviously different expression levels. These proteins were expressed differently between the two types of BPH,or the same type BPH feeding on the two rice varieties with different resistant levels. 27 proteins were selected to investigate the expression level of the corresponding genes by fluorescent quantitative real-time PCR. The results revealed that most of the genes have the same expression trends with their coding proteins. It is noteworthy that three genes(vitellogenin,fatty acid synthase,protein kinase) were expressed at an extremely different pattern,and probably involved in regulating biotype formation and/or the acute stress reaction. This study will help in intensifying the knowledge of the interaction mechanism of BPH and rice,including: (1) during the acute stress reaction of BPH to host rice resistance,the variation laws of the midgut proteomics,which will indirectly provide some important information about the resistance mechanism of rice;(2) the discrepancy between different biotype BPH midgut proteomics,which will provide some important information for division of biotype and elucidating the molecular mechanism of the formation of a special biotype.