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mRNA Expression of basic fibroblast growth factor from A Single Intratracheal Instillation of Papain induced Emphysema in Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第4期21卷 297-300页

作者：傅娟徐永健张珍祥 Department of Respiratory Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

The relations between mRNA expression of basic fibroblast growth factor (bFGF) and the changes in collagen Ⅰand collagen Ⅲ in pulmonary tissues from a single intratracheal instillation of papain induced emphysema in rats were investigated. Wistar rats ( n =42) were randomly divided into normal group and emphysema model 1, 3, 5, 7, 15, 30 day groups ( n =6 in each group). The rat model of emphysema was induced by a single intratracheal instillation of papain. The results of immunohistochemistry SABC and in situ hybridization with bFGF probe were quantitatively analyzed to examine the changes of collagen Ⅰand collagen Ⅲ and bFGF mRNA expression in lung tissues and the percent of positive expression of bFGFmRNA in alveolar macrophages. The results were as follows: (1) In the emphysema model groups the optical densities of collagen Ⅰand collagen Ⅲ began to increase after 3 days, reached the highest at the 7 th day, and began to reduce at the 15 th day; (2) No expression of bFGFmRNA in pulmonary tissues was detectable in the normal group. The positive expression of bFGFmRNA was detectable in lung tissues one day after the intratracheal instillation of papain. The average optical densities reached the peak (41.895±7.017) at the 7th day, significantly higher than in the normal group (0.581±0.139, P <0.01). The positive expression of bFGFmRNA in lung tissues began to reduce at the 15th day; (3) Positive expression of bFGFmRNA in alveolar macrophages of instillation papain rats was detectable 3 days after the intratracheal instillation of papain, and reached the highest at the 7th day with the percent of positive expression of bFGF mRNA in alveolar macrophages being 70.13±11.21, higher than in the normal group (5.12±0.18, P <0.01); (4) The expression of bFGF mRNA in the lung tissues and macrophages was postively related with the changes in collagen Ⅰ and collagen Ⅲ ( P <0.01 or P <0.05) respectively. It was suggested that the up regulation of bFG

关键词： emphysema basic fibroblast growth factor pulmonary interstitial fibrosis

The Effect of Hypoxia on Expression of basic fibroblast growth factor in Pulmonary Vascular Pericytes

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2000年第4期20卷 265-267页

作者：王林熊密车东媛刘绍春郝春荣郑晓静 Department of Pathology School of Basic Medical Sciences Tongji Medical University Wuhan Department of Nephrology Tongji Hospital Tongji Medical University Wuhan

To examine whether hypoxia exerts effect on the expression of basic fibroblast growth ac- tor (bFGF) in pulmonary vascular pericytes (PC), cell culture, in .citu hybridization with probe of digoxigenin-11-dUTP-labled cDNA, immunocytochemistry and image analysis were employed in this study. The results showed that the expression amount of bFGF mRNA and protein in PC of hypoxia (H) group was 1.31 times (P<0. 01) and 1. 17 times (P<0. 01) that of normoxia (N) group re- spectively. It suggests that hypoxia can directly enhance the expression of bFGF mRNA and protein in PC. Increased expression of bFGF may play an important role in the process of PC proliferation and differentiation of PC into smooth muscle-like cells.

关键词： hypoxia basic fibroblast growth factor pulmonary hypertension pericytes

Effect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro

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Chinese Journal of Traumatology 2004年第1期7卷 42-44页

作者：沈雁李斯明唐毅钟灿灿梁佩红陈鸿辉 Guangzhou Red Cross Hospital

Objective: To investigate the effect of basic fibroblast growth factor (bFGF) and hyaluronic acid (HA) on the proliferation of rabbit chondrocytes in vitro. Methods: Chondrocytes from the knee joints of New Zealand white rabbits were cultured. bFGF or HA or both were added into the culture medium respectively, and the proliferation of the chondrocytes was measured with MTT 3 (4, 5 dimethylthiazol 2 yl) 2, 5 diphenyl tetra zolium bromide. (MTT, Sigma, M2128). Results: basic fibroblast growth factor (10 ng/ml) with low concentration of fetal bovine serum in the culture medium promoted the proliferation of chondrocytes significantly, and this effect reached its maximum when concentration of bFGF reached 50 ng/ml. HA itself had no effect on the proliferation of chondrocytes. However, when bFGF was used in combination with HA, especially when the concentration of bFGF was 50 500 ng/ml and that of HA was 10 50 ng/ml, the effect on the proliferation of chondrocytes was much more than when bFGF or HA was used alone. Conclusions: bFGF can promote the proliferation of chondrocytes. HA, which has no effect on the proliferation of the cells, can maintain a normal growth of chondrocytes. When bFGF is used in combination with HA, more proliferation is obtained.

关键词： basic fibroblast growth factor Hyaluronic acid Chondrocytes

Expression of basic fibroblast growth factor Results in the Decrease of Myostatin mRNA in Murine C2C12 Myoblasts

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 697-703页

作者： Hua-Zhong LIU~(1,2) Qing LI~1 Xing-Yuan YANG~1 Lin LIU~1 Lei LIU~1 Xiao-Rong AN~1* Yong-Fu CHEN~1 1 State Key Laboratory for Agrobiotechnology,College of Biological Science,China Agricultural University,Beijing 100094,China 2 Modern Biochemistry Center,Guangdong Ocean University,Zhanjiang 524088,China StateKeyLaboratoryforAgrobiotechnology CollegeofBiologicalScienceChinaAgriculturalUniversityBeijing100094China ModernBiochemistryCenter GuangdongOceanUniversityZhanjiang524088China

During the development and regeneration of skeletal muscle,many growth factors,such asbasic fibroblast growth factor (bFGF,FGF-2) and myostatin,have been shown to play regulating *** contributes to promote proliferation and to inhibit differentiation of skeletal muscle,whereas myostatinplays a series of contrasting *** order to elucidate whether the expression of bFGF has any relationshipwith the expression of myostatin in skeletal muscle cells,we constructed a eukaryotic expression vector forthe expression of exogenous bFGF in murine C2C12 *** RT-PCR assays indicated thatwith the increase of the expression of exogenous bFGF gene,the expression of endogenous myostatin genewas suppressed at mRNA level and protein level.

关键词： basic fibroblast growth factor myostatin murine C2C12 myoblast real time RT-PCR

The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands

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International Journal of Oral Science 2016年第1期8卷 16-23页

作者： Fumitaka Kobayashi Kenichi Matsuzaka Takashi Inoue Department of Clinical Pathophysiology Tokyo Dental CollegeTokyoJapan

This study developed an animal model of surgically wounded submandibular glands （SMGs） and investigated the effects of collagen gel with basic fibroblast growth factor （bFGF） on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF （bFGF group） or without bFGF （control group）. In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day （AOD） 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, g-smooth muscle actin （αSMA）, CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin 19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.

关键词： animal model basic fibroblast growth factor collagen immunohistochemistry salivary gland wound healing

Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia

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Neural Regeneration Research 2009年第2期4卷 111-117页

作者： Xuesong Xing Weili Lü Liguang Sun Department of Biochemistry and Molecular Biology College of Basic Medicine China Medical University Shenyang 110001 Liaoning Province China Department of Anatomy College of Basic Medicine Shenyang Medical College Shenyang 110034 Liaoning Province China

BACKGROUND： The Wnt/β-catenin signaling pathway plays an important role in neural development. ,β-catenin is an important component of the Wnt/β-catenin signaling pathway. The Wnt signaling pathway has been shown to regulate the interaction of neural stem cells with the extracellular matrix. OBJECTIVE： To investigate the effects of basic fibroblast growth factor （bFGF） on β-catenin protein and mRNA expression, and on hippocampal neural stem cell proliferation in a rat model of cerebral ischemia/reperfusion. DESIGN, TIME AND SETTING： A randomized, controlled, neurobiology experiment was performed in Shenyang Medical College between August 2006 and August 2008. MATERIALS： A total of 72 healthy male Wistar rats, aged 3 months, were used in this study. bFGF was provided by Beijing SL Pharmaceutical Co.,Ltd., China. METHODS： Rats were randomly divided into 3 groups： sham-operated, ischemia/reperfusion, and bFGF-treated （n = 24 per group）. Focal cerebral ischemia/reperfusion was induced in rats from the ischemia/reperfusion group and the bFGF-treated group by 2 hour right middle cerebral artery occlusion and 2 hour restoration of blood flow using the suture method. The ischemia/reperfusion and bFGF-treated groups were intraperitoneally administered 500 IU/mL of bFGF, or the same volume of physiological saline, once a day at postoperative days 1 3, and once every 3 days thereafter. Simultaneously, the sham-operated group underwent experimental procedures identical to the ischemia/reperfusion and bFGF-treated groups, with the exception of ischemia/reperfusion induction and drug administration. At 2 hours, 2, 6, 13, and 20 days after ischemiaJreperfusion induction, 50 mg/kg bromodeoxyuridine （BrdU） was administered to each group, twice daily, to label proliferating neural stem cells. MAIN OUTCOME MEASURES： The effects of bFGF on BrdU labeling, and ,8 -catenin mRNA and protein expression, in neural stem cells were examined by immunohistochemistry, Western blot, RT-PCR, and

关键词： basic fibroblast growth factor β-catenin neural stem cell proliferation rats

In vitro characteristics of poly(lactic-co-glycolic acid)microspheres incorporating gelatin particles loading basic fibroblast growth factor

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Acta Pharmacologica Sinica 2006年第6期27卷 754-759页

作者： Shao-hong LI~(1,4)Shao-xi CAI~(1,3)Bing LIU~1 Kai-wang MA~1 Zhen-ping WANG~1 Xiao-kun LI~2 1 Key Laboratory for Biomechanical and Tissue Engineering,State Ministry of Education,College of Bioengineerin,Chongqing University,Chongqing 400030,China 2 Gene Medicine Engineering Research Center,Jinan University,Guangzhou 510632,China KeyLaboratoryforBiomechanicalandTissueEngineering StateMinistryofEducationCollegeofBioengineerinChongqingUniversityChongqing400030China GeneMedicineEngineeringResearchCenter JinanUniversityGuangzhou510632China

Aim:To construct a sustained drug release system for basic fibroblast growthfactor(bFGF).With this special system,bFGF can be used to repair an injuredperipheral nerve,injured spinal cord,or as a carrier for other drugs that need to bereleased over a long ***:Microsphere composite was prepared byencapsulating bFGF into gelatin particles with poly(lactic-co-glycolic acid)(PLGA)as its *** encapsulation was conducted by a phase ***:The average diameter of the gelatin particle-PLGA microspherecomposite was 5-18μm,and bFGF-loading efficiency was up to 80.5%.The bFGFreleasing experiment indicated that this new composite system could release bFGFcontinuously and protect bFGF from ***:A modifiedapproach was successfully employed to develop a biodegradable system for sus-tained release of the drug of bFGF in vitro.

关键词： poly(lactic-co-glycolic acid) gelatin basic fibroblast growth factor microsphere delayed-action preparations

Effect of electro-acupuncture on basic fibroblast growth factor protein and mRNA expression in hippocampal dentate gyrus of spleen deficiency rats

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Neural Regeneration Research 2010年第2期5卷 126-131页

作者： Zhuoxin Yang Yuanyuan Zhuo Haibo Yu Min Pi Mumin Shao Department of Acupuncture and Moxibustion Shenzhen Hospital of Traditional Chinese Medicine Shenzhen 518033 Guangdong Province China Shenzhen Hospital of Traditional Chinese Medicine Guangzhou University of Chinese Medicine Shenzhen 518033 Guangdong Province China Department of Pathology Shenzhen Hospital of Traditional Chinese Medicine Shenzhen 518033 Guangdong Province China

BACKGROUND： Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen, pancreas, intestines, and nervous system in modern medicine. OBJECTIVE; To test whether electro-acupuncture could alter basic fibroblast growth factor （bFGF） protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. DESIGN, TIME AND SETTING： The randomized, controlled, in vivo animal experiment was performed at the National LeveI-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine, between March and November in 2008. MATERIALS： Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co. Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical. Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies, China. Huatuo Brand electroacupuncture instrument （type SDZ-II） was purchased from Suzhou Medical Appliance factory, China. METHODS： A total of 96 male Sprague Dawley rats were randomly assigned to control （n = 32） and induction （n = 64） groups. Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb. The successful models were randomized into two groups： model and electro-acupuncture, with 32 rats in each group. Electro-acupuncture was administered at Zusanfi （ST 36） and Sanyinjiao （SP 6） acupoints using a condensation wave and rarefaction （condensation wave 15 Hz） at a strength of 6-15 V for 20 minutes, once per day. The appearance of a slight shiver in the corresponding locus was taken as the standard. According to electro- acupuncture time points, each group was assigned to four subgroups at 7, 14, 28, and 49 days, respectively, with eight rats in each subgroup. Immunohistochemical staining, image analysis, and reverse-transcription polymerase chain reaction were performed at different time points. MAIN OUTCOME MEASURES： bFGF protein and mRNA expression in t

关键词： spleen deficiency electro-acupuncture basic fibroblast growth factor mRNA protein nerve factor neural regeneration

Infant guinea pig retina model of glutamate toxicity and intervention of basic fibroblast growth factor

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Neural Regeneration Research 2011年第7期6卷 534-541页

作者： Yunzhi Shi Lihua Wei Mingshan Song Min Chen Changqing Du Baoliang Sun Department of Histology and Embryology Taishan Medical College Taian 271000 Shandong Province China Department of Urology Taian Central Hospital Taian 271000 Shandong Province China Department of Foreign Language Taishan Medical College Taian 271000 Shandong Province China Key Laboratory of Cerebral Microcirculation at the Universities of Shandong Department of Neurology Affiliated Hospital of Taishan MedicalCollege Taian 271000 Shandong Province China

Impaired vision with oligemic ophthalmopathy is a result of excitotoxicity caused by excitatory amino acids, resulting in pathological changes, such as loss of retinal neurons and in particular retinal ganglionic cells. The present study utilized infant guinea pigs, aged 45-50 days, to establish injury models via intraperitoneal injection of fixed sodium glutamate doses. Results from hematoxylin- eosin staining revealed significantly reduced retinal ganglionic cell numbers and retinal damage at 10 days after 7 consecutive days of 3 g/kg sodium glutamate treatment; these animals served as the injury model group. In addition, models of moderate injury （glutamate 3 g/kg daily, for 7 consecutive days） were intraperitoneally pretreated with basic fibroblast growth factor （800 U/kg daily）. Immunohistochemistry results confirmed reduced anti-apoptotic gene bcl-2 expression in the ganglion cell layer of glutamate-injured guinea pigs. Expression of the pro-apoptotic gene caspase-3 was increased in the ganglion cell layer and inner plexiform layer. Somatostatin expression was primarily distributed in the ganglion cell layer and inner nuclear layer. Expression of the presynaptic element synaptophysin was weak. However, following basic fibroblast growth factor injection, expressions of the above-described bioactive molecules were reversed, which suggested that basic fibroblast growth factor exerted protective effects on sodium glutamate-induced retinal injury in infant guinea pigs by regulating expression of synaptophysin, somatostatin, Bcl-2, and caspase-3.

关键词： synaptophysin somatostatin apoptosis-related genes basic fibroblast growth factor glutamate retina