Effect of basic fibroblast growth factor on cat corneal endothelial cell proliferation

作     者：Wen-Juan Luo Yang Zhou Mei-Guang Liu and Chuan-Fu Wang 

作者机构：Department of Ophthalmology the Affiliated Hospital of Medical College Qingdao University Qingdao 266003 Shandong Province China 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2011年第4卷第4期

页      面：384-387页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：basic fibroblast growth factor corneal endothelial cells modified tertrozalium salt assay 

摘      要：AIM: To investigate the function of basic fibroblast growth factor (bFGF) on cat corneal endothelial cells proliferation. METHODS: Cat corneal endothelial cells were primarily cultured, stimulated with bFGF for different period, the proliferation of cells was assayed by modified tertrozalium salt (MTT) method, and the morphologic changes were observed with inverted phase contrast microscope and transmission electron microscope. RESULTS: At 1, 3 and 5 days after bFGF was added to cat corneal endothelial cells, the result of MTT in 490nm showed significant difference than that in control group, and the difference was most significant in 10ng/mL group. CONCLUSION: bFGF can promote proliferation of cat corneal endothelial cells. 10ng/mL is the relatively most effective dose.