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A self-powered biosensor based on triboelectric nanogenerator for dual-specificity bacterial detection

InfoMat 2024年第3期6卷 117-130页

作者： Wei Lu Xiaoqiang Wang Congyu Wang Ke Gong Jiawei Li Xue Li Peng Wang State Key Laboratory of Heavy Oil Processing College of Chemistry and Chemical EngineeringChina University of Petroleum(East China)Qingdaothe People's Republic of China Institute of Oceanology Chinese Academy of SciencesQingdaothe People's Republic of China School of Material Science and Engineering China University of Petroleum(East China)Qingdaothe People's Republic of China State Key Laboratory of Microbial Technology Shandong UniversityQingdaothe People's Republic of China Guangxi Academy of Sciences Nanningthe People's Republic of China

Pathogenic and corrosive bacteria pose a significant risk to human health or economic *** specific,sensitive,and on-site detection of these bacteria is thus of paramount significance but remains *** inspiration from immunoassays with primary and secondary antibodies,we describe here a rational design of microbial sensor(MS)under a dualspecificity recognition strategy using Pseudomonas aeruginosa(***)as the detection *** the MS,engineered aptamers are served as the primary recognition element,while polydopamine-N-acetyl-D-galactosamine(PDA-Gal NAc)nanoparticles are employed as the secondary recognition element,which will also generate and amplify changes in the output voltage *** achieve self-powering capability,the MS is constructed based on a triboelectric nanogenerator(TENG)with the specific aptamers immobilized on the TENG electrode *** as-prepared MS-TENG system exhibits good stability in output performance under external forces,and high specificity toward ***,with no cross-reactivity observed.A linear relationship(R^(2)=0.995)between the output voltage and *** concentration is established,with a limit of detection estimated at around 8.7×10^(3) CFU mL^(-1).The utilization of PDA-Gal NAc nanoparticles is found to play an important role in enhancing the specific and reliability of detection,and the underlying mechanisms are further clarified by computational *** addition,the MS-TENG integrates a wireless communication module,enabling real-time monitoring of bacterial concentration on mobile *** work introduces a pioneering approach to designing self-powered smart microbial sensors with high specificity,using a double recognition strategy applicable to various bacteria beyond ***.

关键词： bacterial detection high specificity microbial sensor triboelectric nanogenerator wireless transmission

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Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

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Journal of Pharmaceutical Analysis 2010年第2期22卷 97-101页

作者： Qin Xiao1,2,Hui Chen2,Jin-Ming Lin2 1. College of Ocean,Hebei Agricultural University,Qinhuangdao 066003 2. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China. College of Ocean Hebei Agricultural University Qinhuangdao 066003 China Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Department of Chemistry Tsinghua University Beijing 100084 China

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase,equipped with a polyhistidine affinity tag,was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108,and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein,the ATP content of bacteria was 9.48×10-16mol/mL,and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together,our results provided a simple and efficacious method of the preparation of recombinant luciferase,which could be applied in the determination of bacteria via ATP bioluminescence.

关键词： ATP bioluminescence bacterial detection expression firefly luciferase purification

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Sniffing Bacteria with a Carbon-Dot Artificial Nose

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Nano-Micro Letters 2021年第7期13卷 138-152页

作者： Nitzan Shauloff Ahiud Morag Karin Yaniv Seema Singh Ravit Malishev Ofra Paz-Tal Lior Rokach Raz Jelinek Department of Chemistry Ben Gurion University of the Negev84105 Beer ShevaIsrael Department of Biotechnology Engineering Ben Gurion University of the Negev84105 Beer ShevaIsrael Chemistry Department Nuclear Research CenterNegevP.O.Box 900184190 Beer ShevaIsrael Department of Software and Information System Engineering Ben-Gurion University of the NegevBeer ShevaIsrael Ilse Katz Institute for Nanotechnology Ben Gurion University of the Negev84105 Beer ShevaIsrael

Continuous,real-time monitoring and identification of bacteria through detection of microbially emitted volatile molecules are highly sought albeit elusive *** introduce an artificial nose for sensing and distinguishing vapor molecules,based upon recording the capacitance of interdigitated electrodes(IDEs)coated with carbon dots(C-dots)exhibiting different *** of the C-dot-IDEs to volatile molecules induced rapid capacitance changes that were intimately dependent upon the polarities of both gas molecules and the electrode-deposited *** deciphered the mechanism of capacitance transformations,specifically substitution of electrode-adsorbed water by gas molecules,with concomitant changes in capacitance related to both the polarity and dielectric constants of the vapor molecules *** C-dot-IDE gas sensor exhibited excellent selectivity,aided by application of machine learning *** capacitive C-dot-IDE sensor was employed to continuously monitor microbial proliferation,discriminating among bacteria through detection of distinctive“volatile compound fingerprint”for each bacterial *** C-dot-IDE platform is robust,reusable,readily assembled from inexpensive building blocks and constitutes a versatile and powerful vehicle for gas sensing in general,bacterial monitoring in particular.

关键词： Carbon dots bacterial detection bacterially emitted volatile molecules Capacitive gas sensors Gas polarity

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Magnetorheological elastomer and smartphone enable microfluidic biosensing of foodborne pathogen

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Chinese Chemical Letters 2023年第8期34卷 254-258页

作者： Gaozhe Cai Yuhe Wang Yingchao Zhang Lingyan Zheng Jianhan Lin Key Laboratory of Agricultural Information Acquisition Technology Ministry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing 100083China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Beijing Engineering and Technology Research Center of Food Additives Beijing Technology&Business University(BTBU)Beijing 100048China

Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne *** this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and ***,micropump and microvalves were constructed by deforming the MRE under magnetic actuation and integrated into the microfluidic biosensor for fluidic ***,the micropump was used to deliver immune porous gold@platinum nanocatalysts(Au@PtNCs),bacterial sample,and immunomagnetic nanoparticles(MNPs)into a micromixer,where they were mixed,incubated and magnetically separated to obtain the Au@PtNC-bacteria-MNP *** 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide were injected and catalyzed by the Au@PtNCs,smartphone was used to measure the color of the catalysate for quantitative analysis of target *** optimal conditions,this biosensor could detect Salmonella typhimurium quantitatively and automatically in 1 h with a linear detection range of 8.0×10^(1) CFU/mL to 8.0×10^(4) CFU/mL and a detection limit of 62 CFU/*** microfluidic biosensor was compact in size,simple to use,and efficient for detection,and might be used for in-field screening of foodborne pathogens to prevent food poisoning.

关键词： Magnetorheological elastomer Microfluidic chip Colorimetric biosensor bacterial detection Smartphone image processing

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A Pseudopaline Fluorescent Probe for the Selective detection of Pseudomonas aeruginosa

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CCS Chemistry 2021年第9期3卷 2405-2417页

作者： Tianhu Zhao Jian Zhang Xiaowan Han Jun Yang Xin Wang Maarten Vercruysse Hai-Yu Hu Xiaoguang Lei Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringSynthetic and Functional Biomolecules CenterPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871 State Key Laboratory of Bioactive Substances and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050 Roche Pharma Research&Early Development(pRED) Roche Innovation Center Basel4070 Basel

The rise of resistance to all known antibiotics is a global *** addition to novel treatment options,there is an urgent need to develop rapid,specific,sensitive,and reliable diagnostic methods to detect pathogenic bacteria in clinical samples and reduce the overuse and misuse ***,a metallophore produced by the human pathogen Pseudomonas aeruginosa,transports divalent metal ions via a dedicated active transport system,making it an ideal carrier for a second functional moiety.

关键词： bacterial detection Pseudomonas aeruginosa metallophore fluorescent probe Gram-negative bacteria

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