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The comparison of digital gene expression tag profiling between nurses and foragers of apis mellifera

21世纪第三届全国蜂业科技与海峡两岸蜂产业发展大会暨首届北京密云蜂产业发展高峰论坛

作者： Kang Wang Ting Ji Zheng-Guo Liu Yangzhou University Shandong University

The management of overwintering honeybee colonies is pivotal for the apiculture industry in *** investigate the molecular mechanisms involved in the seasonal regulation of the development of apis mellifera carnica hypopharyngeal gland(HG),transmission electron microscopy(TEM) and large-scale proteomics(iTRAQ) were employed.A total of 3,025 proteins were identified at two-time points:winter(constituting group A) and early spring(constituting group B).Among them,24 proteins were quantified and compared to identify their differential expression *** TEM profiles and iTRAQ analysis results might aid in understanding the molecular mechanisms associated with the seasonal changes in honeybee *** example,the acinar cells of spring worker honeybees had more mature ***,the ribosomal structures and protein biosynthesis were ***,the amount of exocrine royal jelly(RJ) produced could not have satisfied the demand of the feeding larvae and *** might suffer reduced immune function during their feeding *** present study elucidates the regulatory mechanisms of HG on overwintering and spring arousal in *** results might provide a practical and theoretical guideline for the overwintering management program of the apicultural industry.

关键词： apis mellifera overwintering hypopharyngeal gland morphology proteomics differential expression

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The comparison of digital gene expression tag profiling betw...

Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees（apis mellifera）

第四届全国生物信息学与系统生物学学术大会

作者：刘芳李文峰李志国张少吾陈盛禄苏松坤杭州浙江大学动物科学学院澳大利亚国立大学环境医学生物学院生物科学研究所

The honey bee is a highly social insect acknowledged as one of the model organisms to study the mechanisms and evolution of social ***,there were still large unknown on the molecular basis controlling these complicate... 详细信息

关键词： apis mellifera Digital Gene Expression tag profiling(DGE) Differential expression Illumina sequencing Honey bee

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Identification of the proteome complement of hypopharyngeal ...

热带地区小蜜蜂(apis florea)和西方蜜蜂(apis mellifera)的种间竞争

中国养蜂学会蜜蜂饲养管理专业委员会第十三次学术研讨会

作者： Jianke Li~(a,*)Mao Fenga Zhaohui Zhang~b Yinghong Pan~b a Institute of Apicultural Research,Chinese Academy of Agricultural Science,Beijing 100093,China b Institute of Crop Science,Chinese Academy of Agricultural Sciences,The National Key Facility for Crop Gene Resources and Genetic Improvement,12 Zhongguancun Nandajie,Beijing 100081,China

＜正＞The protein complement of the hypopharyngeal gland（HG）of winter worker bees from a strain of apis mellifera artificially selected for increased royal jelly yield and Carnica winter worker bees was *** were partially identified using two-dimensional gel electrophoresis（2-DE）.MALDI-TOF MS and protein engine identification tools that were utilized for the honeybee *** identified proteins in the two bees strains were assigned to major royal jelly（RJ）proteins（MRJPs）.Marked differences were found in the heterogeneity of the MRJPs,in particular *** of the proteins,α-glucosidase and glucose oxidase,were related to carbohydrate metabolism and *** the first time in the HG of honeybees,two proteins, peroxiredoxin and thioredoxin peroxidase,which are related to antioxidation functions,and actin 5C,a major cytoskeletal actin protein which may supply enough actin for normal function of cells, have been *** suggest that the HGs serve a storage function in winter and that during winter the period the HG of high RJ producing bees store more proteins than those of Camica bees.

关键词： apis mellifera winter bees hypopharyngeal gland proteome

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中国蜂业 1977年第3期 31-35页

作者： N.科涅格尔周崧

前言关于利用生物学方法控制南美洲的“非洲蜂”与防止它通过中部美洲迁入美国的问题有过很多不同的争论。可是据我所知还没有人提出过利用最小的蜂种(apis florea)进行这项控制的方案。我对于美洲的情况没有什么亲身经验,“非洲蜂”对... 详细信息

前言关于利用生物学方法控制南美洲的“非洲蜂”与防止它通过中部美洲迁入美国的问题有过很多不同的争论。可是据我所知还没有人提出过利用最小的蜂种(apis florea)进行这项控制的方案。我对于美洲的情况没有什么亲身经验,“非洲蜂”对于美国的养蜂事业实际上是否构成严重的危险,对此我不愿意发表什么不同的看法。我们曾在Oberursel做了一个时

关键词：西方蜜蜂 apis florea apis mellifera 热带地区种间竞争种间斗争生存竞争

蜜蜂(apis mellifera)毒组份——多肽溶血毒melittin的降血胆固醇作用

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蜜蜂杂志 1981年第2期 12-14页

作者：钱锐陈志媛中国科学院昆明动物研究所

多肽溶血毒melittin（以前曾误译'蜜素'）是蜂毒的主要组份,约占全毒乾重的50%,它是1952年用电泳法从乾的溶血毒F1中,与磷脂酶A分离开而得到的.分子量2840,由26个氨基酸组成.

关键词：组份蜂毒蜂产品血胆固醇 apis mellifera melittin

蜜蜂(apis mellifera)工蜂及蜂王卵黄的产生与转移到蜂王的可能性

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蜜蜂杂志 1981年第4期 32-32页

作者： W.Ruty M.Luscher 钱锐瑞士伯尼尔大学动物系动物生理专业瑞士伯尼尔大学动物系动物生理专业

介绍apis mellifera 蜂王24小时蜂内产卵的总重量等于她的体重(平均约180mg)。Nolan 1925)。这意味着蜂王必须合成大量的卵黄蛋白,在数量上仅有大腹的白蚁皇后能超过。Engels(1971)提出蜂王可能从工蜂接受卵黄物质,这种异型合成的蛋白... 详细信息

介绍apis mellifera 蜂王24小时蜂内产卵的总重量等于她的体重(平均约180mg)。Nolan 1925)。这意味着蜂王必须合成大量的卵黄蛋白,在数量上仅有大腹的白蚁皇后能超过。Engels(1971)提出蜂王可能从工蜂接受卵黄物质,这种异型合成的蛋白并入卵母细胞,补偿蜂王有限的蛋白合成。Engels(1972)表明:卵黄原在蜂王上合成的比率充分解释了合并入卵中比率,但有时它也表明:蜂王得力的助手—工蜂也合成卵黄原,因此从工蜂转移蛋白补充蜂王的问题还有待探讨。研究这个问题的程序是我们用免疫实验了工蜂及蜂王卵黄原的同一性,检查了各日龄工蜂的效价,后来我们研究了卵黄转移的可能性、工蜂分泌

关键词： apis mellifera 卵黄蛋白卵黄物质 Nolan 蛋白合成卵母细胞免疫扩散舌腺血淋巴种卵

用5’LongSAGE标签产生的长cDNA片段分析西方蜜蜂(apis mellifera)基因座LOC727225的选择性剪接

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中国畜牧兽医文摘 2012年第10期 27-27页

作者：孙亮先欧阳昊郑华军福建省高校分子生物学与药物化学重点实验室/泉州师范学院

选择性剪接是真核生物产生蛋白多样性的一个重要机制,并能通过产生不同的剪接本来调节基因在不同组织或发育阶段的表达而发挥其作用。本研究采用3条5’LongSAGE标签序列作为上游引物,通过RT-PCR克隆了西方蜜蜂

关键词：选择性剪接西方蜜蜂 LOC727225 apis mellifera cDNA 基因座标签

Spatial distribution of Vespa velutina individuals hunting at domestic honeybee hives： heterogeneity at a local scale

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Insect Science 2014年第6期21卷 765-774页

作者： Karine Monceau Olivier Bonnard Jerome Moreau Denis Thiery INRA UMR1065 Sante et Agroecologie du Vignoble F-33883 Villenave d'Omon ISVV UMR1065 Sante et Agroecologie du Vignoble Bordeaux Sciences Agro Universite de Bordeaux F-33883 Villenave d'Omon Equipe Ecologie Evolutive UMR 6282 Biogeosciences 6 Bd Gabriel Universite de Bourgogne F-21000 Dijon France

Since its recent introduction into Europe, the yellow-legged hornet, Vespa velutina, has become a major predator of the domestic honeybee, apis mellifera, but little is known about its hunting behavior. We studied V. velutina hunting behavior by a capture- mark-recapture procedure in an experimental apiary. A total of 360 hornets were captured and tagged, and we determined：（i） the number of hornets visiting the apiary and the changes in time, （ii） the average number of individual visits per half-day and the time elapsed between consecutive recaptures, and （iii） the individual and global distribution of the hornets in the apiary. More than 50% of the marked hornets were recaptured at least once, this increased to 74% in considering the first marked individuals. We estimated 350 hornets visiting the patch daily with at least 1 visit per half-day. The number of marked hornets decreased over time while the number of unmarked ones increased, suggesting a turnover of individuals. The reduction of the delay between consecutive visits indicates that hornets became more efficient over time. Most of the hornets （88%） were recaptured in front of different hives but, overall, the global distribution was aggregative. Hornets were mainly recaptured in front of 1 hive which was neither the smallest nor the biggest colony, suggesting that the major cue used by hornets is not the amount of food. We hypothesize that the defensive behavior of the honeybee colony could explain our results which may be promising to further studies.

关键词： apis mellifera capture-mark-recapture learning predation Vespidae yellow-legged hornet

Changing climate patterns risk the spread of Varroa destructor infestation of African honey bees in Tanzania

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Ecological Processes 2020年第1期9卷 553-563页

作者： Richard A.Giliba Issa H.Mpinga Sood A.Ndimuligo Mathew M.Mpanda School of Life Sciences and Bio-Engineering The Nelson Mandela African Institution of Science and TechnologyP.O.Box 447ArushaTanzania Beekeeping Training Institute P.O.Box 62TaboraTanzania Centre for Ecological and Evolutionary Synthesis(CEES) Department of BiosciencesUniversity of OsloOsloNorway Dar es Salaam Tanzania

Background:Climate change creates opportune conditions that favour the spread of pests and diseases outside their known active *** climate change scenarios is oftentimes useful tool to assess the climate analogues to unveil the potential risk of spreading suitability conditions for pests and diseases and hence allows development of appropriate responses to address the impending *** the current study,we modelled the impact of climate change on the distribution of Varroa destructor,a parasitic mite that attacks all life forms of honey bees and remains a significant threat to their survival and productivity of bee products in Tanzania and ***:The data about the presence of *** were collected in eight regions of Tanzania selected in consideration of several factors including potentials for beekeeping activities,elevation(highlands ***)and differences in climatic conditions.A total of 19 bioclimatic datasets covering the entire country were used for developing climate scenarios of mid-century 2055 and late-century 2085 for both rcp4.5 and *** thereafter modelled the current and future risk distribution of *** using ***:The results indicated a model performance of AUC=0.85,with mean diurnal range in temperature(Bio2,43.9%),mean temperature(Bio1,20.6%)and mean annual rainfall(Bio12,11.7%)as the important *** risk projections indicated mixed responses of the potential risk of spreads of ***,exhibiting both decrease and increases in the mid-century 2055 and late-century 2085 on different ***,there is a general decline of highly suitable areas of *** in mid-and late-century across all scenarios(rcp4.5 and rcp8.5).The moderately suitable areas indicated a mixed response in mid-century with decline(under rcp4.5)and increase(under rcp8.5)and consistent increase in late *** marginally suitable areas show a decline in mid-century and increase in late-centu

关键词： apis mellifera Apiculture Varroa destructor Honey bee diseases

Influence of visual targets and landmarks on honey bee foraging and waggle dancing

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Insect Science 2020年第2期27卷 349-360页

作者： Bahram Kheradmand Julian Cassano Selena Gray James C.Nieh Division of Biological Sciences Section of EcologyBehavior&EvolutionUniversity of California San DiegoLa JollaCaliforniaUSA

Animals use diverse sensory stimuli to navigate their environment and to recognize rewarding food *** bees use visual atributes of the targeted food source,such as its color,shape,size,direction and distance from the hive,and the landmarks around it to navigate during *** transmit the location information of the food source to other bees if it is highly *** investigate the relative importance of these attributes,we trained bees to feeders in two different *** the first experiment,we asked whether bees prefer to land on(a)a similar feeder at a different distance on the same heading or on(b)a visually distinct feeder located at the exact same *** found that,within a short foraging range,bees relied heavily on the color and the shape of the food source and to a lesser extent on its distance from the *** the second experiment,we asked if moving the main landmark or the feeder(visual target)influenced recruitment dancing for the *** found that foragers took longer to land and danced fewer circuits when the location of the food source,or a major landmark associated with it,*** results demonstrate that prominent visual atributes of food sources and landmarks are evidently more reliable than distance information and that foraging bees heavily utilize these visual cues at the later stages of their journey.

关键词： apis mellifera foraging leaning navigation visual orientation