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antiviral effects of natural small molecules on aquatic rhabdovirus by interfering with early viral replication

Zoological Research 2022年第6期43卷 966-976页

作者： Yan Zhou Tian-Xiu Qiu Yang Hu Lei Liu Jiong Chen State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Ningbo UniversityNingboZhejiang 315211China Laboratory of Biochemistry and Molecular Biology School of Marine SciencesMeishan CampusNingbo UniversityNingboZhejiang 315832China Key Laboratory of Applied Marine Biotechnology of Ministry of Education Meishan CampusNingbo UniversityNingboZhejiang 315832China

Spring viremia of carp virus(SVCV)is globally widespread and poses a serious threat to aquatic ecology and aquaculture due to its broad host range.To develop effective agents to control SVCV infection,we selected 16 naturally active small molecules to assess their anti-SVCV activity.Notably,dihydroartemisinin(DHA)(100μmol/L)and(S,S)-(+)-tetrandrine(TET)(16μmol/L)exhibited high antiviral effects in epithelioma papulosum cyprinid(EPC)cells,with inhibitory rates of 70.11%and 73.54%,respectively.The possible antiviral mechanisms were determined as follows:1.Preincubation with DHA and TET decreased viral particle infectivity in fish cells,suggesting that horizontal transmission of SVCV in the aquatic environment was disrupted;2.Although neither had an effect on viral adhesion,TET(but not DHA)interfered with SVCV entry into host cells(>80%),suggesting that TET may have an antiviral function in early viral replication.For in vivo study,both agents enhanced the survival rate of SVCV-infected zebrafish by 53.3%,significantly decreased viral load,and modulated the expression of antiviralrelated genes,indicating that DHA and TET may stimulate the host innate immune response to prevent viral infection.Overall,our findings indicated that DHA and TET had positive effects on suppressing SVCV infection by affecting early-stage viral replication,thus holding great potential as immunostimulants to reduce the risk of aquatic rhabdovirus disease outbreaks.

关键词： Spring viremia of carp virus Natural small molecules antiviral effects Virus internalization Danio rerio

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In Vivo and in Vitro antiviral effects of Berberine on Influenza Virus

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Chinese Journal of Integrative Medicine 2011年第6期17卷 444-452页

作者：吴莹李季倩金叶智吴珺王谦郝钰 Key Laboratory of Chinese Medicine on Viral Diseases Ministry of Education Microbiology and Immunology DepartmentBeijing University of Chinese Medicine

Objective：To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo.Methods：In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells.In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice.The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality.On days 2,4,and 6,the viral titers in the lungs were determined by hemagglutination assay;hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha（TNF-α） and monocyte specific chemoattractant molecule （MCP-1） were measured by radio immunoassay or enzyme-linked immunosorbent assay;the concentrations of nitric oxide（NO） and inducible nitric oxide synthetase（iNOS） were detected by colorimetric method;reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-αand MCP-1.Results： Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus,with the therapeutic index 9.69.In vivo,berberine decreased mice mortality from 90%to 55%,reduced virus titers in the lungs on day 2 postinfection（P0.05）.The lung histology scores were 1.50±0.67,4.50±1.00,and 5.50±1.00 in the berberine group on days 2,4,and 6,respectively,which were significantly reduced compared to 2.17±0.22,6.83±0.44,and 8.50±0.33 in the infected group（P0.05）.The productions of NO and iNOS were repressed by berberine compared with those in the infected group（P0.01）.The transcription and expression of TNF-αwere inhibited by berberine on day 4（P0.01） and day 6（P0.05）,and those of MCP-1 were inhibited on day 6（P0.01） compared with the infected group.Conclusions：Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo.The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection,as well as

关键词： in vivo in vitro antiviral effects berberine influenza virus

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antiviral effects of Compound Traditional Chinese Medicine on Newcastle Disease Virus

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Animal Husbandry and Feed Science 2010年第4期2卷 29-31页

作者： LIU Qun DUAN Liang-liang JIANG Ping-kang College of Life Science and Technology Southwest University for Nationalities Chengdu 610041 China

[Objective] To investigate the mechanism of compound traditional Chinese medicine （TCM） on Newcastle disease virus （NDV） and to provide a scientific basis for the reasonable usage of antiviral drugs in clinic. [Method] The compound TCM was composed of Hedyotis diffusa, Lonicera japonica Thunb, Radix astragali and Glycyrrhiza uralensis. Different dilutions of fluid extract were prepared. Its antiviral effects on NDV were observed through three inoculation ways, first, inoculation with the medicine and NDV mixture which had been incubated at 37 ℃; second, incubating chicken embryo fibroblasts （CEF） with the medicine followed by inoculation with NDV; third, inoculation with N DV followed by incubating CEF with the medicine. The A,= was determined by M]-r [ 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） ~ method. Therapeutic indexes were used to evaluate the antiviral effects. [ Result] The minimum effective concentration of the compound TCM which acted through the three ways was 1.0 × 2^-10 1.0 × 2^-8 and 1.0 × 2^-7 g/ml, respectively. The antiviral effects of the compound TCM were the best through inoculation with the incubated medicine and NDV mixture, followed by the second method and the third method. [ Conclusion] The compound TCM can not only kill NDV directly in vitro but also inhibit viral propagation.

关键词： Compound traditional Chinese medicine antiviral effects Chicken embryo fibroblasts Newcastle disease virus

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Use of hydroxychloroquine and azithromycin combination to treat the COVID-19 infection

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World Journal of Experimental Medicine 2022年第3期12卷 44-52页

作者： Jyoti Bajpai Akshyaya Pradhan Ajay Kumar Verma Surya Kant Department of Respiratory Medicine King George's Medical UniversityLukcnowLucknow 226003Uttar PradeshIndia Department of Cardiology King George's Medical UniversityLukcnowLucknow 226003Uttar PradeshIndia Department of Respiratory Medicine King George Medical UniversityLucknow 226003Uttar PradeshIndia

Coronavirus disease 2019(COVID-19)infection is unequivocally the worst crisis in recent decades,which is caused by a severe acute respiratory virus 2.Currently,there is no effective therapy for the COVID-19 infection.Different countries have different guidelines for treating COVID-19 in the absence of an approved therapy for COVID-19.Therefore,there is an imminent need to identify effective treatments,and several clinical trials have been conducted worldwide.Both hydroxychloroquine[HCQS],chloroquine,and azithromycin(AZ)have been widely used for management based on in vitro studies favoring antiviral effects against the COVID-19 virus.However,there is evidence both in favor and against the use of hydroxychloroquine and azithromycin(HCQS+AZ)combination therapy to manage the COVID-19 infection.The combination of hydroxychloroquine and azithromycin was significantly associated with increased adverse events.However,the inference of these findings was from observational studies.Therefore,large randomized trials are imperative to show the future path for the use of HCQS+AZ combination therapy.However,owing to the ban on HCQS use in COVID-19,this may no longer be essential.This review is on the pharmacology,trials,regimens,and side effects of hydroxychloroquine and azithromycin combination therapy.

关键词： Hydroxychloroquine Azithromycin antiviral effects QT interval Randomized controlled trial

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Anti-viral and anti-inflammatory effects of kaempferol and quercetin and COVID-2019:A scoping review

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Asian Pacific Journal of Tropical Biomedicine 2021年第8期11卷 327-334页

作者： Mohammad Reza Khazdair Akbar Anaeigoudari Gabriel A.Agbor Cardiovascular Diseases Research Center Birjand University of Medical SciencesBirjandIran Department of Physiology Jiroft University of Medical SciencesJirofIran Center for Research on Medicinal Plants and Traditional Medicine Institute of Medical Research and Medicinal Plants StudiesMinistry of Scientific Research and InnovationsYaoundeCameroon

Severe acute respiratory syndrome coronavirus type 2(SARS-CoV-2)is a novel coronavirus identified at the end of 2019.It is recognized as the causative agent of coronavirus disease 2019(COVID-19).Flavonoids have been shown to exhibit therapeutical effect on complications related to COVID-19.The present study reviews possible therapeutic benefits of flavonoids on SARS-CoV-2.The Web of Science,PubMed,Scopus,and Google Scholar were searched using keywords:“COVID-19”,“SARS-CoV-2”,“Kaempferol”and“Quercetin”in the Title/Abstract.Relevant published articles in the English language until August 2020 were considered.Kaempferol and quercetin showed antiviral properties such as inhibition of protein kinase B and phosphorylation of protein kinase and blocking effects on a selective channel(3a channel)expressed in SARS-CoV infected cells.They also reduced the level of reactive oxygen species,expression of inducible nitric oxide synthase,pro-inflammatory mediators including TNF-α,IL-1α,IL-1β,IL-6,IL-10,and IL-12 p70,and chemokines.Kaempferol and quercetin might exert beneficial effects in the control or treatment of COVID-19 because of their antiviral,antioxidant,anti-inflammatory,and immunomodulatory effects.

关键词： SARS-CoV-2 Flavonoids Kaempferol Quercetin Immunomodulation Anti-inflammatory antiviral effects

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Gig1,a novel antiviral effector involved in fish interferon response

Gig1,a novel antiviral effector involved in fish interferon ...

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细胞—生命的基础——中国细胞生物学学会2013年全国学...

作者： Fan Sun Yi-Bing Zhang Jun Jiang Jian-Fang Gui State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences

Vertebrate interferon(IFN) response defenses against viral infection through the induction of hundreds of IFN-stimulated genes(ISGs).Most ISGs are conserved across vertebrates and presumed to possess similar antiviral... 详细信息

关键词： Gig1 expression regulation RLR signaling pathway antiviral effects IFN response

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