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L-Arginine/nanofish bone nanocomplex enhances bone regeneration via antioxidant activities and osteoimmunomodulatory properties

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Chinese Chemical Letters 2021年第1期32卷 234-238页

作者： Liping Huang Jianhua Zhang Xianhu Liu Tianbao Zhao Zhipeng Gu Yiwen Li School of Materials Science and Engineering Xihua UniversityChengdu 610039China College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China Research Institute of Sun Yat-sen University in Shenzhen Shenzhen 518057China National Engineering Research Center for Advanced Polymer Processing Technology Zhengzhou UniversityZhengzhou 450002China

In this study,a promising strategy has been developed to promote bone regeneration by combining antioxidant activities and osteoimmunomodulatory properties.Herein,an L-arginine/nanofish bone(Arg/NFB) nanocomplex has been prepared and evaluated in vitro and in vivo.The Arg/NFB nanocomplex possesses good antioxidant activities and could modulate the polarization of non-activated macrophage into different types and induce the secretion of pre-inflammato ry,anti-inflammatory,osteogenic as well as angiogenic cytokines.Additionally,the regulated immune microenvironment can enhance the osteogenic differentiation of mouse embryo osteoblast precursor cells(MC3 T3-E1) and angiogenic capacity of human umbilical vein endothelial cells(HUVECs),leading to the improved formation of mineralized nodules,alkaline phosphatase activity and angiogenic effects.In vivo results with cranial defect models reveal that the treatment of Arg/NFB nanocomplex exhibited significant improvement of new bone formation and angiogenesis.All the results demonstrate Arg/NFB nanocomplex with antioxidant activities and osteoimmunomodulatory properties could be a new idea for developing the next generation of bone regeneration biomaterials.

关键词： antioxidant activities Osteoimmunodulatory properties Bone regeneration Arginine Nanofish bone

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Chemical components from Crotalaria pallida Ait. and their antioxidant activities

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Asian Journal of Traditional Medicines 2022年第1期17卷 1-8页

作者： Shuai Shen Ming Bai Shaojiang Song Department of Natural Products Chemistry Key Laboratory of Structure-Based Drug Design and DiscoveryMinistry of EducationSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016PR China

Nine compounds were isolated from the 95%ethanol extract of the dried seeds of Crotalaria pallida.The structures of all compounds were identified through the analysis of spectral data.All compounds were isolated for the first time from Crotalaria genus.Compounds 1-5 were evaluated for their antioxidant abilities by ABTS,DPPH and FRAP assays.Results showed that compounds 1-5 had moderate antioxidant activities。

关键词： Crotalaria pallida Ait separation and purification structural analysis antioxidant activities

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Comparative analysis of antioxidant activities of essential oils and extracts of fennel(Foeniculum vulgare Mill.)seeds from Egypt and China

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Food Science and Human Wellness 2019年第1期8卷 67-72页

作者： Adel F.Ahmed Mengjin Shi Cunyu Liu Wenyi Kang Medicinal and Aromatic Plants Researches Department Horticulture Research InstituteAgricultural Research CenterEgypt Joint International Research Laboratory of Food&Medicine Resource Function Henan ProvinceKaifeng 475004China National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China

The present study was conducted to assay the antioxidant activities of essential oils and ethanol extracts of fennel(Foeniculum vulgare Mill.)seeds from Egypt and China.The major components of the Egyptian fennel essential oil were estragole(51.04%),limonene(11.45%),l-fenchone(8.19%)and trans-anethole(3.62%)by GC–MS analysis.Whereas,the major constituents of Chinese fennel essential oil were transanethole(54.26%),estragole(20.25%),l-fenchone(7.36%)and limonene(2.41%).The fennel seed extracts from Egypt and China contained appreciable levels of total phenolic contents(42.24 and 30.94 mg PE/g,respectively).The extracts exhibited good DPPH radical scavenging capacity with IC50(6.34 and 7.17 mg/g),respectively.A high variation in free radical scavenging activities of essential oils was observed.The Chinese fennel essential oil showed high activity in DPPH radical scavenging with IC50(15.66 mg/g).The Egyptian fennel essential oil showed very low activity with IC50(141.82 mg/g).The results of the present investigation demonstrated significant variations in the antioxidant activities of fennel essential oils and extracts from Egypt and China.

关键词： Foeniculum vulgare Essential oils GC-MS Total phenolic contents antioxidant activities

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antioxidant activities of Exopolysaccharides Produced by Lactic Acid Bacteria Isolated from Commercial Yoghurt Samples

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Advances in Microbiology 2020年第8期10卷 359-374页

作者： Aminat O. Adelekan Taiwo O. Olurin Abiola O. Ezeani Department of Chemical and Food Sciences Bells University of Technology Ota Nigeria

An antioxidant is a substance that inhibits the oxidation of other molecules caused by free radicals. The inbuilt antioxidant systems possessed by living organisms are generally not enough to prevent them from oxidative damages and the uses of synthetic antioxidants also have some harmful effects. This study was aimed at evaluating the antioxidant activities of exopolysaccharides produced by lactic acid bacteria isolated from yoghurt. Lactic acid bacteria (LAB) were isolated from six different brands of commercially available yoghurt using deMan Rogosa Sharpe (MRS) agar. The LAB isolates were identified based on morphological and biochemical analyses and were screened for exopolysaccharide (EPS) production. The LAB isolates screened positive were used for EPS production in a liquid medium and the EPS produced were purified and quantified using standard methods. Antioxidant activities of the EPS were evaluated by determining the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric ion reducing power, and total phenolic contents. Data obtained were analysed using Analysis of Variance. Total lactic acid bacterial count obtained from the yoghurt samples ranged from 0 - 3.9 × 104 CFU/mL with sample A

关键词： antioxidant activities Exopolysaccharides Lactic Acid Bacteria Commercial Yoghurt

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Effects of different drying methods on phenolic components and in vitro hypoglycemic activities of pulp extracts from two Chinese bayberry(Myrica rubra Sieb.et Zucc.)cultivars

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Food Science and Human Wellness 2022年第2期11卷 366-373页

作者： Zhe Liu Jiagan Zhang Shengmin Lu Weimin Tang Yibin Zhou Siew Young Quek Institute of Food Science Zhejiang Academy of Agricultural SciencesHangzhou 310021China School of Tea and Food Technology Anhui Agricultural UniversityHefei 230036China Food Science School of Chemical SciencesThe University of AucklandAuckland 1010New Zealand Riddet Institute Centre of Research Excellence for Food ResearchPalmerston North 4474New Zealand

The effects of two different drying methods,namely hot-air drying and freeze drying,on the phenolic profiles,antioxidant and hypoglycemic activities of pulp extracts from two main Chinese bayberry cultivars(’Biqi’or’BQ’and’Dongkui’or’DK’)were evaluated.The results showed that’BQ’bayberry provided higher total phenolic(TPC),total flavonoid(TFC)and total anthocyanin(TAC)contents than’DK’bayberry after the same drying method,while its antioxidant and hypoglycemic activities were highly affected by drying method.Freeze-dried samples possessed significantly higher TPC,TFC and TAC,and exhibited more potent antioxidant and hypoglycemic activities than hot-air dried ones(Pantioxidant activities and a-amylase inhibitory activity,but higher a-glucosidase inhibition than freeze-dried’BQ’bayberry(Pactivities of bayberry pulp extract.All these results demonstrate that freeze drying is more suitable for the preservation of phenolic components in the pulp extract of Chinese bayberry,and the pulp extract of freeze-dried’DK’could be applied as a potential hypoglycemic food additive.

关键词： Chinese bayberry Drying Phenolic components antioxidant activities In vitro hypoglycemic activities

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Bioactive profile of borno brown Vigna unguiculata grains as influenced by pre-harvest synthetic chemicals

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Food Bioscience 2022年第1期45卷 223-231页

作者： Temitope Omolayo Fasuan Nneka Nkechi Uchegbu Titilope Modupe Olagunju Oyenike Olubunmi Falola Department of Animal Health and Production Technology Federal College of Animal Health and Production TechnologyIbadanNigeria Department of Food Science and Technology University of NigeriaNsukkaNigeria Department of Agricultural and Environmental Engineering Obafemi Awolowo UniversityIle-IfeNigeria

This study investigated the impact of pre-harvest synthetic chemical treatments on the post-harvest bioactive contents,pasting,colour,and thermal properties of borno brown V.unguiculata(L.)Walp grains.Seeds of borno brown V.unguiculata(L.)Walp was planted on an already ploughed,harrowed and ridged plot of land with a portion cultivated as organic and another non-organic.After 90 days,mature grains were harvested and analyzed using standard procedures.Results showed that a total of 44.33 g bioactive compounds/100g grain was present in the organic borno brown V.unguiculata grains whereas,20.56 g bioactive compounds/100g grain was present in the non-organic(control).The identified bioactive compounds in the organic grains included[heneicosane(4.50g/100g grain)];[12-Methyl-E,E-2,13-octadecadien-1-ol(4.09/100g grain)];[3-(2-Hydroxy-6-methylphenyl)-4(3H)-quinazolinone(7.40 g/100g grain)];[Methaqualone(9.08 g/100g grain)].The non-organic contained[heneicosane(4.07g/100g grain)];[3-(2-Hydroxy-6-methylphenyl)-4(3H)-quinazolinone(5.09 g/100g grain)];[Methaqualone(10.61 g/100g grain)].Flavonoids,alkaloids,total phenolic,2,2-diphenyl-1-picryl-hydrazl(DPPH)and ferric ion reducing antioxidant power(FRAP)contents of the organic grains were 19 mgRE/100g,3.40 mg/100g,1550 mgGAE/100g,2840μmolTEAC/100g,and 20.08 mmol Fe^(2+)/100g,respectively.The non-organic grains had flavonoids(11.31 mgRE/100g),alkaloids(1.86 mg/100g),total phenolic(1110 mgGAE/100g),DPPH(1779μmolTEAC/100g)and FRAP(12.24 mmol Fe^(2+)/100g).The peak viscosity of 111.52 RVU,final viscosity(141.25 RVU)and pasting temperature(82.01℃)were obtained for the organic grains while non-organic had peak viscosity of 93.10RVU,final viscosity(95.20 RVU)and pasting temperature(80.10℃).The colour parameters L^(*),a^(*),b^(*)and ΔE were 73.10,0.68,14.47 and 12.98 for the organic and 84.25,0.30,21.10,and 0.00,respectively for non-organic grains.The thermal property showed enthalpy(ΔH)and peak temperature(T_(p))of 3.12 J/g and 78.25℃ for organic,and 2.14 J/g and 76.28℃ for non-organ

关键词： Borno brown Vigna unguiculata grains Organic Bioactive compounds Phenolic compound profile antioxidant activities Pasting property

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Three New Dimers and Two Monomers of Phenolic Amides from the Fruits of Lycium barbarum and Their antioxidant activities

Three New Dimers and Two Monomers of Phenolic Amides from th...

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2014年中国药学大会暨中国药师周

作者： TANG Hai-feng GAO Kai MA Dong-wei Institute of Materia Medica Fourth Military Medical University Department of Pharmacy Xijing HospitalFourth Military Medical University Shaanxi Ark Pharmaceutical Co.Ltd.

Three new dimers of phenolic amides,named lyciumamides A（3）,B（4）and C（5）,along with two known monomers,N-E-coumaroyl tyramine（1）and N-E-feruloyl tyramine（2）,were isolated from the fruits of Lycium barbarum for the first time,with help of the activity-guided chromatographies.Phenolic amides were also firstly found in the genus Lycium.Their structures were determined using spectral analysis and comparing with literature values.Compounds 1–5 were evaluated for their antioxidant activities in scavenging DPPH free radical and inhibiting lipid peroxidation in rat liver microsomes induced by ascorbate/Fe2+,cumine hydroperoxide or CCl4/reduced form of nicotinamide-adenine dinucleotide phosphate,and the results showed that all of them exhibited strong activities while compounds 1 and 2 were more potent than the reference BHA.The data supported that these phenolic amides played an important role on the natural antioxidant properties of the fruits of L.barbarum,similar to other phenolic constituents such as flavonoids and phenolic acids of the fruits.In conclusion,the results complemented the current knowledge of the antioxidative composition of the fruits of L.barbarum and confirmed that this agricultural product contained phenolic amide compounds,which might offer interesting potential applications in the food,cosmetic,and pharmaceutical industries.

关键词： Lycium barbarum phenolic acid amides lyciumamide N-E-coumaroyl tyramine N-E-feruloyl tyramine antioxidant activities

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Three new dimers and two monomers of phenolic acid amides from the fruits of Lyciumbarbarum and their antioxidant activities

Three new dimers and two monomers of phenolic acid amides fr...

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全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议

作者： TANG Haifeng GAO Kai MA Dongwei XI Miaomiao Institute of Materia Medica School of PharmacyFourth Military Medical University Department of Pharmacy Xijing HospitalFourth Military Medical University Shaanxi Ark Pharmaceutical Co.Ltd.

Three new dimers of phenolic amides,named lyciumamides A(3),B(4)and C(5),along with two known monomers,N-?-coumaroyl tyramine(1)and N-?-feruloyl tyramine(2),were isolated from the fruits of Lyciumbarbarum for the first time,with help of the activity-guided chromatographies.Phenolic amides were also firstly found in the genus Lycium.Their structures were determined using spectral analysis and comparing with literature values.Compounds 1-5 were evaluated for their antioxidant activities in scavenging DPPH free radical and inhibiting lipid peroxidation in rat liver microsomes induced by ascorbate/Fe,cumine hydroperoxide or CCl/reduced form of nicotinamide-adenine dinucleotide phosphate,and the results showed that all of them exhibited strong activities while compounds 1 and 2 were more potent than the reference BHA.The data supported that these phenolic amides played an important role on the natural antioxidant properties of the fruits of L.barbarum,similar to other phenolic constituents such as flavonoids and phenolic acids of the fruits.1 In conclusion,the results complemented the current knowledge of the antioxidative composition of the fruits of L.barbarum and confirmed that this agricultural product contained phenolic amide compounds,which might offer interesting potentialapplications in the food,cosmetic,and pharmaceutical industries.

关键词： Lyciumbarbarum Phenolic acid amides Lyciumamide N-E-coumaroyl tyramine N-E-feruloyl tyramine antioxidant activities

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antioxidant mechanism of tea polyphenols and its impact on health benefits

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Animal Nutrition 2020年第2期6卷 115-123页

作者： Zhaoming Yan Yinzhao Zhong Yehui Duan Qinghua Chen Fengna Li College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China Laboratory of Animal Nutritional Physiology and Metabolic Process Institute of Subtropical Agriculture Chinese Academy of SciencesKey Laboratory of Agro-ecological Processes in Subtropical RegionHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureChangsha 410125China College of Animal Science South China Agricultural UniversityGuangzhou 510642China Hunan Co-Innovation Center of Animal Production Safety Hunan Collaborative Innovation Center for Utilization of Botanical Functional IngredientsChangsha 410128China

Tea trees have a long history of cultivation and utilization.People in many countries have the habit of drinking tea and choosing green tea,oolong tea,or black tea according to different regions and personal tastes.Tea polyphe nols are a general term for polyphenol co mpounds in tea,and has been shown to have good effects on antioxidant,anti-inflammatory,cancer prevention and regulation of lipid metabolism.Tea polyphenols have been widely used as antioxidants in disease treatment and animal husbandry,but their specific mechanism of action needs to be further clarified and revealed.This review focuses on the definition,classification,antioxidant activity and the regulation of signaling pathways of tea polyphenols.This paper also aims to examine the application of tea polyphenols in human and animal health,providing a scientific basis for this application in addition to proposing future directions for the development of this resource.

关键词： Tea polyphenols antioxidant activities Animal production Signal transduction pathway

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Efficiency of seed bio-priming technique for healthy mungbean productivity under terminal drought stress

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Journal of Integrative Agriculture 2021年第1期20卷 87-99页

作者： Hamid NAWAZ Nazim HUSSAIN Niaz AHMED Haseeb-ur-REHMAN Javaiz ALAM Department of Agronomy The Islamia University of BahawalpurBahawapur 63100Pakistan Department of Agronomy Bahauddin Zakariya UniversityMultan 60000Pakistan Department of Soil Science Bahauddin Zakariya UniversityMultan 60000Pakistan

Recently, drought-induced damaging impact in reducing the crop growth and development is drastically ranked at the top under various abiotic stresses. And especially water stress at the reproductive growth stages termed as terminal drought has become a severe threat for mungbean productivity. To mitigate the drought stress condition, "bio-priming" has emerged as a newly agronomic and sustainable technique in improving the mungbean production. A 2-year field study during Kharif season 2017–2018 was conducted to investigate the efficacy of rhizobacteria seed priming in mungbean(AZRI mung-06), at Agronomic Research Area, Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan. The experiment comprised two factors containing FA(seed treatments, control(dry seeds), hydro-priming, silicon(Si)-priming, and bio-priming(mixture strains of Pseudomonas fluorescens+Rhizobium phaseoli)) and FB(irrigation water-regimes at various growth stages including leaf formation(L), stem elongation(S)+flowering(F)+pod formation(P) containing treatments are normal irrigation(I(L+S+F+P)) and terminal drought stress(I(F+P))). All the treatments were arranged in randomized complete block design under factorial design and were replicated thrice. Results indicated that the exposure of drought stress at flowering and pod formation stages hampered the morpho-physiological growth and yield of mungbean. Nevertheless, seed priming treatments particularly bio-priming were effective in alleviating the detrimental effects of drought stress. Bio-priming significantly increased the yield and yield components(seeds/plant, 1 000-grain weight and harvest index) of mungbean and regulated the activities/levels of antioxidants(superoxide dismutase, catalase, peroxidase, ascorbic acid, and total phenolics) under drought stress. Compared with the control, bio-priming increased the seed yield of mungbean by 8–12% under normal as well as drought stress conditions during both years of study. Bio-priming also improved the nutrient uptake behavior fo

关键词： bio-priming antioxidant activities drought nutrients uptake mungbean

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