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Soybean(Glycine max)rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorusmineralizing-related bacteria in phosphate deficient acidic soils

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Journal of Integrative Agriculture 2024年第5期23卷 1685-1702页

作者： Qianqian Chen Qian Zhao Baoxing Xie Xing Lu Qi Guo Guoxuan Liu Ming Zhou Jihui Tian Weiguo Lu Kang Chen Jiang Tian Cuiyue Liang Root Biology Center State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510642China Henan Academy of Crops Molecular Breeding Henan Academy of Agricultural SciencesZhengzhou 450002China

Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.

关键词： organic phosphorus acid phosphatase soybean bacterial community phoC-harboring bacteria rhizosphere

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Colorimetric Detection of acid phosphatase and Malathion Using NiCo_(2)O_(4)Nanosheets as Peroxidase-Mimicking Activity

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Journal of Analysis and Testing 2024年第2期8卷 170-179页

作者： Jia Chen Zerong Long Yan Li Hongdeng Qiu Xinjiang Uygur Autonomous Product Quality Supervision and Inspection Institute Urumqi830000China CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou730000China

The establishment of a low-cost and facile approach for the assay of acid phosphatase(ACP)and its inhibitor is a research *** this paper,we successfully synthesized NiCo_(2)O_(4)nanosheets with peroxidase-mimicking activity in alkaline deep eutectic solvents(DESs)composed of NaOH and PEG-200,which can catalyze 3,3',5,5'-tetramethylbenzidine(TMB)to the oxidation state TMB(ox-TMB)in the presence of *** to L-ascorbic acid-2-phosphate can be hydrolyzed into ascorbic acid by ACP,the peroxidase-like activity of NiCo_(2)O_(4)nanosheets is ***,the addition of malathion,an inhibitor of ACP,can inhibit the activity of ACP and thus limit the production of ascorbic acid(AA).Therefore,a colorimetric approach can be established for highly sensitive and selective detection of ACP and malathion with detection limits of 0.032 U/L and 0.65 nmol/L,*** importantly,this method can be successfully applied to malathion detection in environmental samples tap water and food cucumber juice,indicating that it has potential application prospects in environmental and agricultural fields.

关键词： NiCo_(2)O_(4)nanosheets acid phosphatase Malathion Peroxidase-mimicking activity Colorimetric

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Soluble protein and acid phosphatase exuded by ectomycorrhizal fungi and seedlings in response to excessive Cu and Cd

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Journal of Environmental Sciences 2009年第12期21卷 1667-1672页

作者： ZHENG Weishuang, FEI Yingheng, HUANG Yi College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China. College of Environmental Sciences and Engineering Peking University Beijing 100871 China

Fungi and their symbionts can alleviate heavy metal stress by exuding soluble proteins and enzymes. This study examined the role of soluble protein and acid phosphatase （APase） exuded by Xerocomus chrysenteron, an ectomycorrhizal fungus, and the seedlings of its symbiont, Chinese pine （Pinus tabulaeformis）, under conditions of excessive Cu and Cd. The growth type showed that this poorly studied ectomycorrhizal fungus was capable of tolerating high concentrations of Cu, and may be useful in phytoremediation. X. chrysenteron grew well at 80 mg/L Cu, and the EC50 for Cd was 17.82 mg/L. X. chrysenteron also showed enhanced exudation of soluble protein in both isolated and inoculated cultivations under the influence of Cu and Cd. Soluble protein exudation, however, differed under Cu and Cd stress in isolates. In mediums containing Cu, soluble protein exudation increased with concentration, but in mediums containing Cd the content of soluble protein increased to a comparable level at all concentrations. This study demonstrated that soluble protein was related to heavy metal tolerance, although the different ions played different roles. While APase activity in exudates of fungi and seedlings decreased under Cu and Cd stress in comparison to the control, the APase activity in seedlings was maintained by inoculation. Thus, X. chrysenteron facilitated the ability of plant to maintain a normal nutrient uptake, and therefore to protect it from heavy metal toxicity.

关键词： soluble protein acid phosphatase exudate Cu, Cd Xerocomus chrysenteron

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A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

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Science China Chemistry 2021年第1期64卷 151-156页

作者： Qiulin Ma Yunlong Chen Nan Feng Feng Yan Huangxian Ju State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Department Clinical Laboratory Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Cancer HospitalJiangsu Institute of Cancer ResearchNanjing 210009China

A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase(ACP).The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol(PEG)modified indium-tin oxide(ITO)*** the presence of ACP,the peptide substrate was dephosphorylated under acidic condition to generate a new mass *** quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide *** optimal detection conditions,the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit(LOD)of 0.04 g/*** designed ACP sensing chip has been used to analyze ACP in complex clinical samples,which exhibited high selectivity,good repeatability,and admirably anti-interference *** work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis,and provides a convenient method for the quantitation of proteases in clinical diagnosis.

关键词： acid phosphatase sensing chip MALDI-MS quantitation proteases mass signal

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Molecular Characterization and Functional Analysis of a New acid phosphatase Gene(Ha-acp1) from Heterodera avenae

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Journal of Integrative Agriculture 2014年第6期13卷 1303-1310页

作者： LIU Yan-ke HUANG Wen-kun LONG Hai-bo PENG Huan HE Wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词： Heterodera avenae acid phosphatase RNA interference

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Effect of acid phosphatase produced by Trichoderma asperellum Q1 on growth of Arabidopsis under salt stress

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Journal of Integrative Agriculture 2017年第6期16卷 1341-1346页

作者： ZHAO Lei LIU Qun ZHANG Ya-qing CUI Qing-yu LIANG Yuan-cun Institute of Environment and Ecology Shandong Normal University Jinan 250014 P.R. China College of Life Science Shandong Normal University Jinan 250014 P.R. China College of Plant Protection Shandong Agricultural University Tai'an 271018 P.R. China

Salt stress is a major environmental factor that inhibits crop *** *** the most efficient biocontrol fungi and some of the strains can stimulate plant *** solubilization is known as one of the main mechanisms in promoting plant growth,but the underlying mechanisms of phosphate solubilization in the salinity still need to be *** Trichoderma asperellum Q1 isolated and identified in our lab is a beneficial rhizosphere biocontrol fungus with a high phosphate solubilization *** could produce acid and alkaline phosphatases when using insoluble organic phosphorus as the sole phosphorus source,the salt stress increased the phosphorus-solubilization ability of the strain and the activities of the two ***,an acid phosphatase was purified from the fermentation broth by ammonium sulphate precipitation,ion-exchange,and gel filtration *** molecular weight was 55 k Da as determined by *** purified acid phosphatase was used to investigate growth performance of Arabidopsis thaliana by plate assay and the result showed that it contributed to Arabidopsis growth by transforming organic phosphate into a soluble inorganic form under salt *** our knowledge,this is the first report on acid phosphatase purification from *** and its function in regulation of plant growth under salt stress.

关键词： Trichoderma asperellum Arabidopsis thaliana acid phosphatase plant-growth promotion salt stress

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In situ growth of polydopamine on surface of covalent organic frameworks under the catalysis of acid phosphatase for dopamine detection

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Chinese Chemical Letters 2021年第11期32卷 3368-3371页

作者： Fei Qu Ziwei Guo Dafeng Jiang Xian-En Zhao The Key Laboratory of Life-Organic Analysis Qufu Normal UniversityQufu 273165China The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine Qufu Normal UniversityQufu 273165China Department of Physical and Chemical Testing Shandong Center for Disease Control and PreventionJi'nan 250014China

Dopamine(DA)is easy to be oxidized and polymerizes to form polydopamine(pDA)in alkaline conditions,while the synthesis is usually time-consuming(48 h).Herein,the polymerization of DA is completed with 4 h under the catalysis of acid phosphatase(ACP).The high efficiency makes the detection of DA feasibility based on the self-polymerization of *** this assay,pDA is grown in situ on the surface of covalent organic frameworks(COFs),and then the fluorescence of COFs is quenched *** linear range of DA is achieved from 0.5-50μmol/L with a detection limit of 0.16μmol/*** detection of DA is not interfered with uric acid,ascorbic acid,and some phenolic compounds,because these substances cannot polymerize in the presence of ***,benefiting from the good sensitivity and selectivity,DA has been successfully determined by this strategy in human urine samples with satisfactory recoveries.

关键词： Fluorescence Enzyme Polydopamine Dopamine acid phosphatase

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Comparison of Conformational Changes and Inactivation of Penaeus Penicillatus acid phosphatase during Unfolding by Urea

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Tsinghua Science and Technology 1998年第3期3卷 3-8页

作者：陈素丽陈清西杨佩真林达廷李森周海梦 Department of Biology Xiamen University Xiamen 361005 Department of Biological Science and Biotechnology Tsinghua University Beijing 100084

It has been reported that inactivation occurs before noticeable conformational changes can be detected during denaturation of creatine kinase and other enzymes. Therefore, Tsou suggested that enzyme active sites may display more conformational flexibility than the enzyme molecules as a whole. In the present investigation, the conformational changes of Penaeus penicillatus acid phosphatase during denaturation in urea solutions were studied by following changes in the intrinsic fluorescence, ultraviolet difference absorption, and circular dichroism spectra. Inactivation of the enzyme in urea solutions was compared with unfolding of the enzyme molecule. The results show that the extent of unfolding in guanidine solutions measured by several different methods closely coincides with each other and that slightly lower concentrations of guanidine are required to bring about inactivation than are required to produce significant conformational changes of the enzyme molecule. At the same concentrations, the inactivation rate constants are markedly faster than the rate constants for unfolding of the enzyme. The above results suggest that the active sites of this enzyme display more conformational flexibility than the enzyme molecule as a whole. 

关键词： acid phosphatase inactivation conformational change urea solution

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Effect of Salinity on Germination,Seedling Growth and acid phosphatase Activity in Lettuce

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American Journal of Plant Sciences 2015年第1期6卷 57-63页

作者： Nawel Nasri Issam Saidi Rym Kaddour Mokhtar Lachaal Physiologie et Biochimie de la Tolérance au Sel des Plantes Faculte des Sciences de TunisCampus UniversitaireTunisTunisia

The impact of salt stress (NaCl 100 mM) on two lettuce varieties Romaine and Vista was conducted at germination and early seedling stages. The seeds of lettuce varieties were provided by the Seed Laboratory of Tunisian Ministry of Agriculture. The seeds were germinated in Petri dishes with double filter paper in distilled water (control) or NaCl solution (100 mM) for 5 days. The result showed that salinity significantly affected percentage and rate of germination in Vista variety but 100% of germination was found in Romaine. Length and fresh weight of root and shoot were reduced significantly with salt treatment in two lettuce varieties. Regarding biochemical analysis, acid phosphatase activity in root increased in Romaine and decreased in Vista. In shoot, this activity showed no difference with the control in the two varieties. However in cotyledons, and during 24 hours after germination, salinity decreased acid phosphatase activity in both varieties whereas in the later hours (48 - 96 h) this activity reached the value of the control in Romaine and Vista.

关键词： acid phosphatase Germination Lettuce Salinity Seedling

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Partial Purification and Properties of an acid phosphatase from Pearl Oyster Pinctada Fucata

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Tsinghua Science and Technology 2003年第4期8卷 476-480页

作者：柴云峰谢莉萍张荣庆 Center for Ocean Science and Engineering Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

acid phosphatases (ACPs) are marker enzymes for the detection of lysosomes in cell fractions. However, ACPs in sea creatures are less studied than those on land. An acid phosphatase was partially purified from pearl oyster Pinctada fucata by chromatography on Sephadex G 150 and Con A Sepharose 4B. The specific activity was 1719 U·mg -1 and with optimum pH (5.0) and temperature (60℃). The enzyme was strongly inhibited competitively by product analog WO 3- 4 and MoO 3- 4, but less inhibited by product analog AsO 3- 4. The enzyme could also be strongly inhibited by heavy metal ions, such as Ag + and Cu 2+ , but was not affected by Pb 2+ . High concentrations of ethanol (64%) and NaF (10 -3 mol·L -1 ) could inhibit the enzyme while low concentration of NaF (acid, formaldehyde and dithiothreitol) could inhibit the enzyme. It is concluded that the properties of the enzyme are different from many fresh water mollusks.

关键词： acid phosphatase pearl oyster Pinctada fucata kinetics

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