Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene(Ha-acp1) from Heterodera avenae

作     者：LIU Yan-ke HUANG Wen-kun LONG Hai-bo PENG Huan HE Wen-ting PENG De-liang 

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2014年第13卷第6期

页      面：1303-1310页

核心收录：

学科分类：09[农学] 090402[农学-农业昆虫与害虫防治] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：the fund of the National Basic Research Program of China (2013CB127502) the Special Fund for Agro-Scientific Research in the Public Interest,China (200903040) the National Natural Science Foundation of China (31201493) 

主　　题：Heterodera avenae acid phosphatase RNA interference 

摘      要：For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.