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Journal of Integrative Plant Biology 2015年第11期57卷 954-968页

作者： Hao Du Yu Chang Fei Huang Lizhong Xiong National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural University

Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Gibberellic acid （GA） and abscisic acid （ABA） play critical roles in the developmental programs and environmental responses, respectively, through complex signaling and metabolism networks. However, crosstalk between the two phytohormones in stress responses remains largely unknown. In this study, we report that GIBBERELLIN-INSENSITIVE DWARF 1 （GID1）, a soluble receptor for GA, regulates stomata[ develop- ment and patterning in rice （Oryza sativa L.）. The gidl mutant showed impaired biosynthesis of endogenous ABA under drought stress conditions, but it exhibited enhanced sensi- tivity to exogenous ABA. Scanning electron microscope and infrared thermal image analysis indicated an increase in the stomatal conductance in the gidl mutant under drought conditions. Interestingly, the gidl mutant had increased levels of chlorophyll and carbohydrates under submergence conditions, and showed enhanced reactive oxygen species （ROS）-scavenging ability and submergence tolerance compared with the wild-type. Further analyses suggested that the function of GID1 in submergence responses is partially dependent on ABA, and GA signaling by GID1 is involved in submergence tolerance by modulating carbohydrate consumption. Taken together, these findings suggest GID1 plays distinct roles in stomatal response and submergence tolerance through both the ABA and GA signaling pathways in rice.

关键词： abscisic acid carbohydrateconsumption gibberellic acid stomata Oryza sativa

Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

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Journal of Integrative Plant Biology 2021年第4期63卷 802-815页

作者： Pengcheng Guo Leelyn Chong Fangming Wu Chuan-Chih Hsu Chuanyou Li Jian-Kang Zhu Yingfang Zhu State Key Laboratory of Crop Stress Adaptation and Improvement Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China State Key Laboratory of Plant Genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyInnovation Academy for Seed Designthe Chinese Academy of SciencesBeijing 100101China Institute of Plant and Microbial Biology Academia SinicaTaipei 11529 Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesShanghai 200032China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIndiana 47907USA

MED25 has been implicated as a negative regulator of the abscisic acid(ABA)signaling ***,it is unclear whether other Mediator subunits could associate with MED25 to participate in the ABA ***,we used affinity purification followed by mass spectrometry to uncover Mediator subunits that associate with MED25 in transgenic *** found that at least26 Mediator subunits,belonging to the head,middle,tail,and CDK8 kinase modules,were copurified with MED25 in ***,the tail module subunit MED16 was identified to associate with MED25 under both mock and ABA *** further showed that the disruption of MED16 led to reduced ABA sensitivity compared to the wild *** analysis revealedthattheexpressionofseveral ABA-responsive genes was significantly lower in med16 than those in wild ***,we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5(ABI5)to positively regulate ABA ***,med16 and med25 mutants displayed opposite phenotypes in ABA response,cuticle permeability,and differential ABI5-mediated EM1 and EM6 ***,our data indicate that MED16 and MED25 differentially regulate ABA signaling byantagonisticallyaffectingABI5-mediated transcription in Arabidopsis.

关键词： ABI5 abscisic acid MED16 MED25 mediator signaling

Genome-wide characterization of early response genes to abscisic acid coordinating multiple pathways in Aegilops tauschii

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The Crop Journal 2021年第4期9卷 934-944页

作者： Yu Wei Liangjing Cao Xucheng Huang Xuan Wang Huan Wang Yongchao Song Qiang He Mingjie Lyu Xinwen Hu Jun Liu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Institute of Tropical Agriculture and Forestry Hainan UniversityHaikou 570228HainanChina Department of Biological Sciences Xi’an Jiaotong-Liverpool UniversitySuzhou 215123JiangsuChina Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

The diploid wild goat grass Aegilops tauschii(Ae. tauschii, 2 n = 14;DD), as the D-sub genome of common wheat, provides rich germplasm resources for many aspects of wheat breeding. abscisic acid(ABA) is an essential phytohormone that plays a pivotal role in plant adaptation to abiotic stresses. However,the gene regulation network of Ae. tauschii in response to ABA stress remains unclear. Here, we conducted a time-course strand-specific RNA-sequencing study to globally profile the transcriptome that responded to ABA treatment in Ae. tauschii. We identified 4818 differentially expressed transcription units/genes with time-point-specific induction/repression patterns. Using functional annotation, one-to-one ortholog and comparative transcriptome profiling analyses, we identified 319 ABA-responsive Ae. tauschii orthologs that were also induced/repressed under ABA treatment in hexaploid wheat. On the quantitative trait loci(QTL) used in wheat marker-assisted breeding, we found that the ABA-responsive expression patterns of eight Ae. tauschii orthologs were associated with drought stress tolerance, flowering process and/or grain quality. Of them, the ABA-responsive gene encoding sucrose:sucrose 1-fructosyltransferase in fructan and glucose metabolism pathways showed the most significant association with wheat drought tolerance. The characterization of ABA early-responsive genes in this study provides valuable information for exploring the molecular functions of the regulatory genes and will assist in wheat breeding.

关键词： Wheat Aegilops tauschii Transcriptome abscisic acid Fructan

Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with abscisic acid Content under Drought Stress

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Journal of Integrative Plant Biology 2009年第2期51卷 175-183页

作者： Gang-Ping Hao Xiu-Hai Zhang Yong-Qin Wang Zhong-Yi Wu Cong-Lin Huang Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China Taishan Medical College Taian 271000 China

Drought tolerance is a comprehensive quantitative trait that is being understood further at the molecular genetic level. abscisic acid （ABA） is the main drought-induced hormone that regulates the expression of many genes related to drought responses. 9-cis-epoxycarotenoid dioxygenase （NCED3） is thought to be a key enzyme in ABA biosynthesis. In this paper, we measured the ABA content increase under drought stress, and sequenced and compared the sequence of AtNCED3 among 22 Arabidopsis thaliana accessions. The results showed that the fold of ABA content increase under drought stress was highly variable among these accessions. High density single nucleotide polymorphism （SNP） and insertion/deletion （indel） were found in the AtNCED3 region, on average one SNP per 87.4 bp and one indel per 502 bp. Nucleotide diversity was significantly lower in the coding region than that in non-coding regions. The results of an association study with ANOVA analysis suggested that the 274th site （P←→S） and the 327th site （P←→R） amino acid variations might be the cause of ABA content increase of 163av accession under drought stress.

关键词： abscisic acid Arabidopsis thaliana association study drought stress NCED3 region nucleotide variation.

Proanthocyanidins Inhibit Seed Germination by Maintaining a High Level of abscisic acid in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2012年第9期54卷 663-673页

作者： Liguo Jia Qiuyu Wu Nenghui Ye Rui Liu Lu Shi Weifeng Xu Hui Zhi A. N. M. Rubaiyath Bin Rahman Yiji Xia Jianhua Zhang Department of Biology Hong Kong Baptist University Hong Kong China School of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Hong Kong China

Proanthocyanidins （PAs） are the main products of the flavonoid biosynthetic pathway in seeds, but their biological function during seed germination is still unclear. We observed that seed germination is delayed with the increase of exogenous PA concentration in Arabidopsis. A similar inhibitory effect occurred in peeled Brassica napus seeds, which was observed by measuring radicle elongation. Using abscisic acid （ABA）, a biosynthetic and metabolic inhibitor, and gene expression analysis by real-time polymerase chain reaction, we found that the inhibitory effect of PAs on seed germination is due to their promotion of ABA via de novo biogenesis, rather than by any inhibition of its degradation. Consistent with the relationship between PA content and ABA accumulation in seeds, PA-deficient mutants maintain a lower level of ABA compared with wild-types during germination. Our data suggest that PA distribution in the seed coat can act as a doorkeeper to seed germination. PA regulation of seed germination is mediated by the ABA signaling pathway.

关键词： abscisic acid Arabidopsis Brassica napus proanthocyanidins seed germination.

A novel impedance immunosensor based on O-phenylenediamine modified gold electrode to analyze abscisic acid

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Chinese Chemical Letters 2010年第4期21卷 472-475页

作者： Li, Qian Wang, Ruo Zhong Huang, Zhi Gang Li, He Song Xiao, Lang Tao Hunan Agr Univ Hunan Prov Key Lab Crop Germplasm Innovat & Utili Hunan Prov Key Lab Phytohormones & Growth Dev Changsha 410128 Hunan Peoples R China

An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA) was *** operating pH,absorption time,absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical *** calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal *** results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng/mL...

关键词： O-Phenylenediamine abscisic acid Immunosensor

Neophaseic acid catabolism in the 90 -hydroxylation pathway of abscisic acid in Arabidopsis thaliana

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Plant Communications 2022年第5期3卷 132-143页

作者： Ya-Li Bai Xiaoming Yin Cai-Feng Xiong Bao-Dong Cai Yan Wu Xiao-Yun Zhang Zhenwei Wei Tiantian Ye Yu-Qi Feng Department of Chemistry Wuhan UniversityWuhan 430072P.R.China State Key Laboratory of Hybrid Rice College of Life SciencesWuhan UniversityWuhan 430072P.R.China Department of Chemistry Lanzhou UniversityLanzhou 730000P.R.China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan 430072P.R.China

abscisic acid(ABA)hydroxylation is an important pathway for ABA inactivation and homeostasis ***,we discover a new downstream catabolite of neophaseic acid(neoPA)in the ABA 90-hydroxyl pathway and identify it as epi-neodihydrophaseic acid(epi-neoDPA)by comparing its accurate mass,retention time,and MSn spectra with those of our chemically synthesized *** of Arabidopsis seed germination and ABA-related gene expression reveal that neoPA rather than epi-neoDPA possesses ABA-like hormonal *** vitro enzyme activity tests of prokaryotic recombinant protein reveal that NeoPAR1(neoPA reductase 1)identified from Arabidopsis converts neoPA into *** mutation at Tyr163 in the conserved motif of NeoPAR1 abolishes the catalytic activity of *** seed germination was observed in NeoPAR1 knockdown and knockout mutants,whereas retarded seed germination and the accumulation of epi-neoDPA and ABA were observed in NeoPAR1 overexpression lines,suggesting that NeoPAR1 is involved in seed germination and maintenance of ABA homeostasis.

关键词： abscisic acid ABA catabolism epi-neoDPA neoPA reductase seed germination phaseic acid

Transcriptome Profiling of abscisic acid-Related Pathways in SNAC4/9-Silenced Tomato Fruits

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Transactions of Tianjin University 2021年第6期27卷 473-486页

作者： Yefang Liu Yanan Zhao Liping Chai Jiaqian Zhou Sen Yang Xiaohong Kou Zhaohui Xue School of Chemical Engineering and Technology Tianjin UniversityTianjin 300350China

The NAC(NAM,ATAF,and CUC)family is considered one of the largest families of plant transcription factor,and its members are involved in fruit *** acid(ABA)has been demonstrated to modulate the fruit ripening *** applying the virus-induced gene silencing method and next-generation sequencing technology,we conducted a compara-tive analysis of the eff ects of SNAC4(SlNAC48,accession number:NM 001279348.2)and SNAC9(SlNAC19,accession number:XM 004236996.2)on tomato fruit *** results of high-throughput sequencing identified 1262 significant(p1.0)related to ABA in SNAC4-silenced and SNAC9-silenced tomatoes,respectively,for further *** XET gene and two other genes(E8 and EXP1)were significantly down and upregulated in SNAC4-silenced tomatoes,***,the PYL9 gene and four other genes(PP2C,CYP707A2,EXPA6,and ACS6)were significantly down and upregulated in SNAC9-silenced tomatoes,*** addition,ten DEGs were selected for use in tests to confirm the accuracy of the transcriptomic results by quantitative real-time polymerase chain reaction(qRT-PCR).Our results highlight the relationship between SNAC4/9 and ABA in the regulation of tomato ripening,which may help provide a theoretical basis for further research on the mechanisms of tomato fruit ripening and senescence.

关键词： Tomato RNA sequencing NAC transcription factor Virus-induced gene silencing abscisic acid

Direct Modulation of the Guard Cell Outward- Rectifying Potassium Channel （CORK） by abscisic acid

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Molecular Plant 2017年第11期10卷 1469-1472页

作者： Amanda Ooi Fouad Lemtiri-Chfieh Aloysius Wong Christoph Gehring Division of Biological and Environmental Sciences and Engineering 4700 King Abdullah University of Science and Technology Thuwa123955-6900 Kingdom of Saudi Arabia

Dear Editor,abscisic acid （ABA） induces turgot loss and hence stomatal closure by promoting rapid net K^＋ efflux from guard cells （GCs） through outward-rectifying K^＋（K^＋out） channels （Schroeder et al., 1987; Blatt, 1990）. The mechanisms of ABA signaling in GCs are detailed elsewhere （see Munemasa et al., 2015; Weiner et al., 2010; Pandey et al., 2007）. Briefly, ABA binds to the PYR/ PYL/RCARs, a family of soluble steroidogenic acute regulatory- related lipid transfer （START） proteins, and, in turn, inactivates the downstream PP2C （type 2C protein phosphatase）, leading to the activation of SnRK2.6 （SNF1 [sucrose non-fermenting-1- related protein kinase]/OST1 [open stomata 1]） protein kinases.

关键词： Direct Modulation of the Guard Cell Outward- Rectifying Potassium Channel (CORK) abscisic acid

Phytohormones Jasmonic acid,Salicylic acid,Gibberellins,and abscisic acid are Key Mediators of Plant Secondary Metabolites

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World Journal of Traditional Chinese Medicine 2021年第3期7卷 307-325页

作者： Zong-You Lv Wen-Jing Sun Rui Jiang Jun-Feng Chen Xiao Ying Lei Zhang Wan-Sheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203 Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003 Department of Pharmaceutical Botany School of PharmacySecond Military Medical UniversityShanghai 200433China

Until recently,many studies on the role of phytohormones in plant secondary metabolism focused on jasmonic acid(JA),salicylic acid(SA),gibberellins(GA),and abscisic acid(ABA).It is now clear that phytohormone?induced regulation of signaling occurs via regulation of the biosynthetic pathway genes at the transcriptional level or through posttranslational regulation,or an increase in secondary metabolite deposition(e.g.,trichomes).Here,we summarize recent advances,updating the current reports on the molecular machinery of phytohormones JA,SA,GA,and ABA involved in plant secondary *** review emphasizes the differences and similarities among the four phytohormones in regulating various secondary metabolic biosynthetic pathways and also provides suggestions for further research.

关键词： abscisic acid gibberellins jasmonic acid salicylic acid secondary metabolism