Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

作     者：Pengcheng Guo Leelyn Chong Fangming Wu Chuan-Chih Hsu Chuanyou Li Jian-Kang Zhu Yingfang Zhu Pengcheng Guo;Leelyn Chong;Fangming Wu;Chuan-Chih Hsu;Chuanyou Li;Jian-Kang Zhu;Yingfang Zhu

作者机构：State Key Laboratory of Crop Stress Adaptation and ImprovementKey Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China State Key Laboratory of Plant GenomicsNational Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyInnovation Academy for Seed Designthe Chinese Academy of SciencesBeijing 100101China Institute of Plant and Microbial BiologyAcademia SinicaTaipei 11529 Shanghai Center for Plant Stress BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesShanghai 200032China Department of Horticulture and Landscape ArchitecturePurdue UniversityWest LafayetteIndiana 47907USA 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第4期

页      面：802-815页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(NSFC 31900238 and NSFC 32070307)to Y.Z 

主　　题：ABI5 abscisic acid MED16 MED25 mediator signaling 

摘      要：MED25 has been implicated as a negative regulator of the abscisic acid(ABA)signaling ***,it is unclear whether other Mediator subunits could associate with MED25 to participate in the ABA ***,we used affinity purification followed by mass spectrometry to uncover Mediator subunits that associate with MED25 in transgenic *** found that at least26 Mediator subunits,belonging to the head,middle,tail,and CDK8 kinase modules,were copurified with MED25 in ***,the tail module subunit MED16 was identified to associate with MED25 under both mock and ABA *** further showed that the disruption of MED16 led to reduced ABA sensitivity compared to the wild *** analysis revealedthattheexpressionofseveral ABA-responsive genes was significantly lower in med16 than those in wild ***,we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5(ABI5)to positively regulate ABA ***,med16 and med25 mutants displayed opposite phenotypes in ABA response,cuticle permeability,and differential ABI5-mediated EM1 and EM6 ***,our data indicate that MED16 and MED25 differentially regulate ABA signaling byantagonisticallyaffectingABI5-mediated transcription in Arabidopsis.