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An aba-binding protein with nucleic acid-binding property

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Science China(Life Sciences) 1998年第2期41卷 209-216页

作者：郑志富金振华周燮夏凯马诚 Agronomy Department Nanjing Agricultural University Nanjing China Institute of Development Biology Chinese Academy of Sciences Beijing China

Maize cDNA expression library was screened using the antibodies against aba binding proteins (ABBP). From 200 000 separated phage plaques,only one cDNA clone coding for a polypeptide was obtained. It shows 60%-65% homology to the genes of nucleic acid binding proteins in ***, about 120 cDNA clones coding maize 17s rRNA were unexpectedly obtained. In view of this, the ABBP purified by aba affinity chromatography and the antibodies derived from the purified ABBP were examined. It is found that rRNA is indeed present in the ABBP. In addition,the obtained antibodies could recognize RNA as well as ABBP. It is suggested that the ABBP have nucleic acid binding property and can form a complex with rRNA.

关键词： aba binding proteins (ABBP) rRNA anti ABBP sera cDNA expression library immunological screening.

aba-Mediated Inhibition of Germination Is Related to the Inhibition of Genes Encoding Cell-Wall Biosynthetic and Architecture： Modifying Enzymes and Structural Proteins in Medicago truncatula Embryo Axis

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Molecular Plant 2009年第1期2卷 108-119页

作者： Christine Gimeno-Gilles Eric Lelievre Laure Viau Mustafa Malik-Ghulam Claudie Ricoult Andreas Niebel Nathalie Leduc Anis M. Limami University of Angers UMR_A 1191 Physiologie Moleculaire des Semences (PMS) 2 Bd Lavoisier F-49045 Angers France UMR 441 CNRs-INRA Laboratoire de Biologie Moleculaire des Relations Plantes Micro-organismes (LIPM) Castanet-Tolosan 31326 cedex France University of Angers UMR Sciences Agronomiques Appliques a I'Horticulture (SAGAH) 2 Bd Lavoisier F-49045 Angers France

Radicle emergence and reserves mobilization are two distinct programmes that are thought to control germination. Both programs are influenced by abscissic acid （aba） but how this hormone controls seed germination is still poorly known. Phenotypic and microscopic observations of the embryo axis of Medicago truncatula during germination in mitotic inhibition condition triggered by 10 μM oryzalin showed that cell division was not required to allow radicle emergence. A suppressive subtractive hybridization showed that more than 10% of up-regulated genes in the embryo axis encoded proteins related to cell-wall biosynthesis. The expression of α-expansins, pectin-esterase, xylogucan-endotransglycosidase, cellulose synthase, and extensins was monitored in the embryo axis of seeds germinated on water, constant and transitory aba. These genes were overexpressed before completion of germination in the control and strongly inhibited by aba. The expression was re-established in the aba transitory-treatment after the seeds were transferred back on water and proceeded to germination. This proves these genes as contributors to the completion of germination and strengthen the idea that cell-wall loosening and remodeling in relation to cell expansion in the embryo axis is a determinant feature in germination. Our results also showed that aba controls germination through the control of radicle emergence, namely by inhibiting cell-wall loosening and expansion.

关键词： aba cell-wall expansion germination Medicago truncatula radicle emergence XET.

Calcineurin-B-Like Protein CBL9 Interacts with Target Kinase CIPK3 in the Regulation of aba Response in Seed Germination

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Molecular Plant 2008年第2期1卷 238-248页

作者： Girdhar K. Pandey John J. Grant Yong Hwa Cheong Beom-Gi Kim Le Gong Li Sheng Luan Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Department of Plant Molecular Biology University of Delhi South Campus Benito Juarez Road New Delhi-110021 India Present address: Department of Bio-Environmental Science Sunchon National University Suncheon Jeonnam 540-742 Korea Present address: Department of Molecular Physiology and Biochemistry National institute of Agricultural Biotechnology Suwon 441-707 Korea

Calcium plays a vital role as a second messenger in many signaling pathways in plants. The calcineurin B-like proteins （CBLs） represent a family of plant calcium-binding proteins that function in calcium signaling by interacting with their interacting protein kinases （CIPKs）. In our previous study, we have reported a role for one of the CBLs （CBL9） and one of the CIPKs （CIPK3） in aba signaling. Here, we have shown that CBL9 and CIPK3 physically and functionally interact with each other in regulating the aba responses. The CBL9 and CIPK3 proteins interacted with each other in the yeast two- hybrid system and when expressed in plant cells. The double mutant cbl9cipk3 showed the similar hypersensitive response to aba as observed in single mutants （cbl9 or cipk3）. The constitutively active form of CIPK3 genetically complemented the cbl9 mutant, indicating that CIPK3 function downstream of CBL9. Based on these findings, we conclude that CBL9 and CIPK3 act together in the same pathway for regulating aba responses.

关键词： aba calcium CBL CIPK osmotic stress signal transduction.

Arabidopsis thaliana RECEPTOR DEAD KINASE1 Functions as a Positive Regulator in Plant Responses to aba

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Molecular Plant 2017年第2期10卷 223-243页

作者： Dhinesh Kumar Ritesh Kumar Dongwon Baek Tae-Kyung Hyun Woo Sik Chung Dae-Jin Yun Jae-Yean Kim Division of Applied Life Sciences Plant Molecular Biology and Biotechnology Research Center Gyeongsang National University Jinju 660-701 Korea Department of Industrial Plant Science and Technology Conege of Agricultural Life and Environmental Sciences Chungbuk National University Cheongju 28644Korea These authors contributed equally to this article.

Abscisic acid （aba） is a major phytohormone involved in important stress-related and developmental plant processes. Membrane-delimited aba signal transduction plays an important role in early aba signaling, but the molecular mechanisms connecting core signaling components to the plasma membrane remain un- clear. Plants have evolved a large number of receptor-like kinases （RLKs） to modulate diverse biological processes by perceiving extracellular stimuli and activating downstream signaling responses. In this study, a putative leucine-rich repeat-RLK gene named RECEPTOR DEAD KINASE1 （AtRDK1） was identified and characterized in Arabidopsis thaliana. RDK1 promoter-GUS analysis revealed that RDK1 is expressed ubiq- uitously in the various tissues in Arabidopsis, and its expression is mainly induced by aba. In the presence of aba, RDKl-deficient rdkl-1 and rdkl-2 lines showed significant resistance in cotyledon greening and root growth, whereas RDKl-overexpressing lines showed enhanced sensitivity. Consistently, the expres- sion of aba-responsive genes was significantly downregulated in rdkl mutant seedlings, which were also hypersensitive to drought stress with increased water loss. Interestingly, RDK1 was found to be an atypical kinase localized to the plasma membrane and did not require its kinase activity during aba-mediated inhi- bition of seedling development. Accordingly, RDK1 interacted in the plasma membrane with type 2C protein phosphatase ABSClSIC ACID INSENSITIVE1 （ABI1）; this interaction was further enhanced by exogenous application of aba, suggesting that RDKl-mediated recruitment of ABI1 onto the plasma membrane is important for aba signaling. Taken together, these results reveal an important role for RDK1 in plant responses to abiotic stress conditions in an aba-dependent manner.

关键词： aba abiotic stress Arabidopsis atypical kinases protein phosphatases receptor-like kinases

Cell biological mechanism for triggering of aba accumulation under water stress in Vicia faba leaves

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Science China(Life Sciences) 2001年第4期44卷 421-428页

作者：张大鹏何芳莲贾文锁 Laboratory of Molecular Developmental Biology of Fruit Trees Laboratory of Plant Physiology and Biochemistry China Agricultural University Beijing China

Water stress-induced aba accumulation is a cellular signaling process from water stress perception to activation of genes encoding key enzymes of aba biosynthesis, of which the water stress-signal perception by cells or triggering mechanism of the aba accumulation is the center in the whole process of aba related-stress signaling in plants. The cell biological mechanism for triggering of aba accumulation under water stress was studied in leaves of Vicia faba. Mannitol at 890 mmol· kg-1 osmotic concentration induced an increase of more than 5 times in aba concentra-tion in detached leaf tissues, but the same concentration of mannitol only induced an increase of less than 40 % in aba concentration in protoplasts. Like in detached leaf tissues, aba concentra-tion in isolated cells increased more than 10 times under the treatment of mannitol at 890 mmol·kg-1 concentration, suggesting that the interaction between plasmalemma and cell wall was essential to triggering of the water stress-induced aba accumulation. Neither Ca2+-che- lating agent EGTA nor Ca2+ channel activator A23187 nor the two cytoskeleton inhibitors, colchicine and cyto-chalasin B, had any effect on water stress-induced aba accumulation. Interestingly water stress-induced aba accumulation was effectively inhibited by a non-plasmalemma-perme- able sulfhy-dryl-modifier PCMBS (p-chloromercuriphenyl-sulfonic acid), suggesting that plasmalemma pro-tein(s) may be involved in the triggering of water stress-induced aba accumulation, and the protein may contain sulfhydryl group at its function domain.

关键词： water stress, aba (abscisic acid), PCMBS (p-chloromercuriphenyl-sulfonic acid), cytoskeleton, Ca2+, Vicia faba.

Osaba8ox2, an aba catabolic gene, suppresses root elongation of rice seedlings and contributes to drought response

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The Crop Journal 2020年第3期8卷 480-491页

作者： Yan Zhang Xiaoping Wang Yanzhong Luo Lan Zhang Yuan Yao Lu Han Zhenhua Chen Lei Wang Yubin Li Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China College of Life Science and Technology Harbin Normal UniversityHarbin 150025HeilongjiangChina

In rice, Osaba8ox encodes abscisic acid(aba) 8′-hydroxylase, which catalyzes the committed step of aba catabolism. The contribution of aba catabolism in root development remains unclear. We investigated the role of Osaba8ox2 in root growth and development and drought response. GUS staining results showed that Osaba8ox2 was expressed mainly in roots at seedling stage and was strongly expressed in the meristematic zone of the radicle. Osaba8ox2 expression in roots was markedly decreased after 0.5 h polyethylene glycol(PEG) treatment and increased after 0.5 h rehydration, implying that Osaba8ox2 is a drought-responsive ***8ox2 knockout mediated by the CRISPR-Cas9 system increased drought-induced aba and indole-3-acetic acid accumulation in roots, conferred increased aba sensitivity, and promoted a more vertically oriented root system architecture(RSA) beneficial to drought ***8ox2 overexpression suppressed root elongation and increased stomatal conductance and transpiration rate. Consequently, Osaba8ox2 knockout dramatically improved rice drought tolerance, whereas Osaba8ox2 overexpression seedlings were hypersensitive to drought stress,suggesting that Osaba8ox2 contributes to drought response in rice. Compared with wild type,functional leaves of Osaba8ox2 knockout seedlings showed higher aba levels, whereas overexpression lines showed lower aba levels, suggesting that Osaba8ox2, as an aba catabolic gene, modulates aba concentration through aba catabolism. Osaba8ox2 and Osaba8ox3 were both localized in the endoplasmic reticulum. Together, these results indicate that Osaba8ox2 suppresses root elongation of rice seedlings, increases water transpiration, and contributes to drought response through aba catabolism, and that Osaba8ox2 knockout dramatically improves rice drought tolerance. They highlight the key role of aba catabolism mediated by Osaba8ox2 on root growth and development. Osaba8ox2, as a novel RSA gene, would be a potential genetic target fo

关键词： aba suppresses root elongation of rice seedlings and contributes to drought response Osaba8ox2 an aba catabolic gene

The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates aba responses in Arabidopsis

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Journal of Integrative Plant Biology 2022年第12期64卷 2361-2373页

作者： Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia Synthetic and Functional Biomolecules Center Bejing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Beijing 100871China

The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress *** RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in ***,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant *** demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(aba)and that ALKBH9 B has m^(6)A demethylation activity on endogenous *** out ALKBH9 B led to hypersensitivity to aba treatment during seed germination and early seedling *** further showed that ALKBH9 B removes the m^(6)A modification in the aba INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following aba treatment,affecting the stability of these ***,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in aba responses depended on ABI3 and *** findings reveal the important roles of the m^(6)A modification in aba responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating aba responses post-transcriptionally.

关键词： aba ALKBH9B Arabidopsis demethylase m^(6)A RNA modification

SlTPP4 participates in aba-mediated salt tolerance by enhancing root architecture in tomato

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Journal of Integrative Agriculture 2023年第8期22卷 2384-2396页

作者： DU Dan HU Xin SONG Xiao-mei XIA Xiao-jiao SUN Zhen-yu LANG Min PAN Yang-lu ZHENG Yu PAN Yu Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/College of Horticulture and Landscape Architecture/Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China Chongqing Vocational Institute of Engineering Chongqing 402260P.R.China

Salinity tolerance is an important physiological index for crop *** are typically the first plant tissue to withstand salt *** this study,we found that the tomato(Solanum lycopersicum)trehalose-6-phosphate phosphatase(SlTPP4)gene is induced by abscisic acid(aba)and salt,and is mainly expressed in *** of SlTPP4 in tomato enhanced tolerance to salt stress,resulting in better growth *** saline conditions,SlTPP4 overexpression plants demonstrated enhanced sucrose metabolism,as well as increased expression of genes related to salt *** the same time,expression of genes related to aba biosynthesis and signal transduction was enhanced or altered,***-depth exploration demonstrated that SlTPP4 enhances Casparian band development in roots to restrict the intake of Na^(+).Our study thus clarifies the mechanism of SlTPP4-mediated salt tolerance,which will be of great importance for the breeding of salt-tolerant tomato crops.

关键词： trehalose-6-phosphate phosphatase(TPP) salt tolerance root aba tomato(Solanum lycopersicum)

Root-derived long-distance signals trigger aba synthesis and enhance drought resistance in Arabidopsis

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Journal of Genetics and Genomics 2024年第7期51卷 749-761页

作者： Qian-Qian Liu Jin-Qiu Xia Jie Wu Yi Han Gui-Quan Zhang Ping-Xia Zhao Cheng-Bin Xiang Division of Life Sciences and Medicine Division of Molecular&Cell BiophysicsHefei National Science Center for Interdisciplinary Sciences at the MicroscaleUniversity of Science and Technology of ChinaThe Innovation Academy of Seed DesignChinese Academy of SciencesHefeiAnhui 230027China College of Life Sciences Anhui Agricultural UniversityHefeiAnhui 230036China College of Agronomy South China Agricultural UniversityGuangzhouGuangdong 510642China School of Biology and Basic Medical Sciences Soochow UniversitySuzhouJiangsu 215123China

Vascular plants have evolved intricate long-distance signaling mechanisms to cope with environmental stress,with reactive oxygen species(ROS)emerging as pivotal systemic signals in plant stress ***,the exact role of ROS as root-to-shoot signals in the drought response has not been *** this study,we reveal that compared with wild-type plants,ferric reductase defective 3(frd3)mutants exhibit enhanced drought resistance concomitant with elevated NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3(NCED3)transcript levels and abscisic acid(aba)contents in leaves as well as increased hydrogen peroxide(H_(2)O_(2))levels in roots and *** experiments distinctly illustrate that drought resistance can be conferred by the frd3 rootstock regardless of the scion genotype,indicating that long-distance signals originating from frd3 roots promote an increase in aba levels in ***,the drought resistance conferred by the frd3 mutant rootstock is weakened by the CAT2-overexpressing scion,suggesting that H_(2)O_(2)may be involved in long-distance ***,the results of comparative transcriptome and proteome analyses support the drought resistance phenotype of the frd3 *** together,our findings substantiate the notion that frd3 root-derived long-distance signals trigger aba synthesis in leaves and enhance drought resistance,providing new evidence for root-to-shoot long-distance signaling in the drought response of plants.

关键词： frd3 Drought resistance Root-to-shoot long-distance signals Reactive oxygen species(ROS) H_(2)O_(2) Iron aba