An ABA-binding protein with nucleic acid-binding property

作     者：郑志富 金振华 周燮 夏凯 马诚 

作者机构：Agronomy Department Nanjing Agricultural University Nanjing China Institute of Development Biology Chinese Academy of Sciences Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：1998年第41卷第2期

页      面：209-216页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：ProjectsupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .396 80 0 15 ) 

主　　题：ABA binding proteins (ABBP) rRNA anti ABBP sera cDNA expression library immunological screening. 

摘      要：Maize cDNA expression library was screened using the antibodies against ABA binding proteins (ABBP). From 200 000 separated phage plaques,only one cDNA clone coding for a polypeptide was obtained. It shows 60%-65% homology to the genes of nucleic acid binding proteins in ***, about 120 cDNA clones coding maize 17s rRNA were unexpectedly obtained. In view of this, the ABBP purified by ABA affinity chromatography and the antibodies derived from the purified ABBP were examined. It is found that rRNA is indeed present in the ABBP. In addition,the obtained antibodies could recognize RNA as well as ABBP. It is suggested that the ABBP have nucleic acid binding property and can form a complex with rRNA.