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Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus

Asian Pacific Journal of Tropical Medicine 2014年第2期7卷 105-112页

作者： Kilani-Jaziri Soumaya Ghedira Zied Nasr Nouha Krifa Mounira Ghedira Kamel Franca Dijoux Marie Genviève Ghedira Chekir Leila Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12.Universite de Monastic Faculte de Pharmacie de Monastir Laboratoire de Biologie Moleculaire et Cellulaire Faculté de Medecine Dentaire de MonastirUniversite de MonastirRue Aricenne5019 MonastirTunisie Laboratoire de Botanique.Pharmacognosie et Phytothérapie UMR CNRS 5557 Ecologie Microbienne Institut des Sciences Pharmaceutiques et Biologiques-Faculte de Pharmacie Université Claude Bernard Btiment Netien-8 Avenue Rockefeller-69373 Lyon cedex 08France

Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(***).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from *** aerial part were *** addition,these extracts were also investigated for their cytotoxic and apoptotic *** major compound of the methanol extract was *** metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay ***,16,8,and 4 mg/mL of each extract were tested to investigate their *** scavenging *** extract(800,*** 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H_2O_2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell *** major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic ***:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase *** same extracts inhibited lipid peroxidation by 61.5%and 42.0%.*** extracts inhibited *** by 27.1%and 25.3%,*** methanol cxtract induced DNA *** was determined as the major compound isolated from the butanol fraction of metlianol ***:It appears that *** extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.

关键词： Polyphenols Orientin Xanthine/Xanthine oxidase assay Lipid peroxidation assay Deoxyribose assay DNA fragmentation

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Systemic PCD occurs in TMV-tomato interaction

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Science China(Life Sciences) 2008年第11期51卷 1009-1019页

作者： ZHOU ShuMin, LIU WenNa, KONG LinAn & WANG Mao College of Biological Sciences, China Agriculture University, Beijing 100094, China College of Biological Sciences China Agriculture University Beijing China

In hypersensitive response (HR), programmed cell death (PCD) is reported as a powerful defense mechanism in plant immune responses to pathogen. However, little is known about the PCD in sys-temic acquired resistance (SAR). Using tobacco mosaic virus (TMV) to infect the tomato (Lycopersicon esculentum cv. Jiafen 16) we found that localized TMV-infection could induce cell death in the uninoculated parts of the tomatoes, where the enzyme-linked immunosorbent assay (ELISA) showed no spreading virus. The biological and molecular characterization of this cell death was shown as fol-lowing: chromatin condensed and formed peripheral conglomeration in nuclei; cell nucleus were TUNEL positive labeled; genomic DNA was fragmented and showed DNA laddering; mitochondria and chloroplast were disrupted; tonoplast and plasma membrane were shrunk and degradated. These re-sults suggested that with an absence of TMV spread, the local TMV-infection on certain tomato leaves could induce systemic PCD in the root-tips, stem-apices and uninoculated leaves. The systemic PCD has various initiation and synchronization in such tissues and is distinct in inducement and exhibition from HR-PCD and SAR.

关键词： programmed cell death systemic PCD TUNEL assay DNA ladder TMV tomato

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DNA damage-induced by sodium flouride (NaF) and the effect ofcholicalciferol

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BIOCELL 2020年第2期44卷 263-268页

作者： VEYSEL YÜKSEK SEMIHA DEDE AYŞE USTA SEDATÇETIN MEHMET TAŞPINAR Van Yuzuncu Yil UniversityÖzalp Vocational High School VanTurkey Department of Biochemistry Faculty of Veterinary MedicineVanYuzuncu Yil UniversityVanTurkey Department of Chemistry Faculty of ScienceVanYuzuncu Yil UniversityVanTurkey Department of Medical Biology Faculty of MedicineVanYuzuncu Yil UniversityVanTurkey

It is known that the high electronegativity of fluorine affects various soft tissues,especially the bone structure in *** these tissues are the kidneys,which play an important role in the excretion of fluoride from the *** affects many cellular *** of these effects is DNA *** study aimed to investigate the likely protective effect of cholecalciferol(vitamin D3)on genomic DNA damage-induced NaF depending on concentration and *** IC25 and IC50 values of NaF for 3,12 and 24 h and optimum dose of increase in proliferation to vitamin D3 through MTT assay in NRK-52E kidney cells were *** damage was significantly increased(p<0.05)compared to the control group in all groups except for vitamin *** was determined that treatment with NaF together with vitamin D3 decreased the DNA damage compared to NaF treated groups for 3 and 12 *** combined with vitamin D3 was determined statistically to decrease(p<0.05)DNA damage compared to NaF treated groups for 24 *** a result,it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased(p<0.05)the genomic DNA damage,but NaF treatment together with vitamin D3 decreased the DNA damage in renal cells depending on the *** was concluded that vitamin D3 may be useful in preventing DNA damage caused by NaF.

关键词： NaF DNA damage Vitamin D3 Comet assay

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Antioxidant activity of newly discovered lineage of marine actinobacteria

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Asian Pacific Journal of Tropical Medicine 2013年第4期6卷 325-332页

作者： Loganathan Karthik Gaurav Kumar Kokati Venkata Bhaskara Rao Environmental Biotechnology Division School of Bio Sciences and TechnologyVIT UniversityVelloreTamil NaduIndia

Objective:To investigate the antioxidant activity of marine ***:The content of total phenolics,the level of antioxidant potential by DPPH radical scavenging activity,metal chelating activity,FRAP method,βcarotene assay and NO scavenging activity in extract were ***:In all the methods the extract exhibited good scavenging activity except NO scavenging *** IC50 values of marine actinobacteria extract on DPPH radical were found to be 41.09μg/*** zone of color retention was 12 mm inβ-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV- photolysed H2O2-driven oxidative damage to *** analysis identified some of the major phenolic compounds in extracts,which might he responsible for the antioxidant potential and *** showed a 100%cytotoxic effect in brine shrimp lethality assay within 10 *** novel actinobacteria was identified as Streptomyces LK-3（JF710608） through I6S rDNA ***:The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.

关键词： Streptomyces LK-3 Antioxidant DPPH radical scavenging assay Cytotoxicity

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Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata(L.) *** ***(Amaranthaceae)

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Asian Pacific Journal of Tropical Medicine 2013年第3期6卷 180-187页

作者： Gaurav Kumar Loganathan Karthik Kokati Vankata Bhaskara Rao Molecular and Microbiology Research Laboratory Environmental Biotechnology DivisionSchool of Bio Sciences and TechnologyVIT UniversityVelloreTamil Nadu-632 014India

Objective:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva Lanata（***） ***:During the preliminary phytochemical analysis,the aqueous extract of *** was screened for the presence of carbohydrates,proteins,phenolic compounds,oil and fats,saponins,flavonoids,alkaloids. tannins and *** activity of the extract was determined by 2.2-dipbenyl- 1-picrylhydrazyl radical scavenging activity,metal chelating activity,reducing power activity and DNA damage inhibition *** of phenolic compounds was performed by FolinCiocaiteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, *** and phytosterols as major phytochemical *** extract exhibited high 2.2—diphenyl-1-picrylhydrazyl radical scavenging activity(IC50= 110.74μg/ mL).metal chelating activity(IC50= 758.17μg/mL).reducing power activity and DIA damage inhibition *** extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid（3,4,3-OH）,apigenin-7—O-glucoside tapigetrin), quercetin-3—O-rutinoside（rutin） and myricetin（3,5,7,3,4,5-OH）by high performance liquid chromatography *** extract was found non toxic towards human erythrocytes in the hemolytic assay(IC50= 24.89 mg/mL).Conclusions:These results conclud that *** stem possesses high antioxidant activity and can he used for the development of natural and sale antioxidant compounds.

关键词： Aerva lanata Antioxidant DPPH radical scavenging assay Phenolic compounds Hemolytic activity

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Anti-proliferative potential of sodium thiosulfate against HT 29 human colon cancer cells with augmented effect in the presence of mitochondrial electron transport chain inhibitors

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Asian Pacific Journal of Tropical Biomedicine 2020年第7期10卷 333-340页

作者： Bhavana Sivakumar Sri Rahavi Boovarahan Gino A Kurian Vascular Biology Lab School of Chemical and BiotechnologySASTRA Deemed UniversityThanjavurIndia

Objective:To compare the anti-proliferative effect of sodium thiosulfate on human colorectal cancer cells(HT-29)and normal small intestine cells(IEC6).Methods:Cells(HT-29 and IEC6)were treated with different concentrations of sodium thiosulfate ranging from 0.5 m M to 80 m M for 24 *** viability was measured via crystal violet and MTT ***-29 cells were further treated in the presence and absence of mitochondrial electron transport chain(ETC)inhibitors,KATP channel opener and closer and H2S inhibitors for 24 h followed by sodium thiosulfate in order to study their respective roles in the anti-proliferative activity of sodium ***:The IC50 values of sodium thiosulfate on HT-29 cells were 40.93 m M and 42.45 m M by crystal violet and MTT assay whereas,in the case of IEC6 cells,the values were 45.17 m M and 47.22 m *** inhibition of endogenous H2S enzymes and KATP channel induced no change in the anti-proliferative capacity of sodium ***,the anti-proliferative activity of sodium thiosulfate was enhanced in the presence of mitochondrial ETC ***:HT-29 cell growth is effectively attenuated by sodium thiosulfate and the anti-proliferative activity of sodium thiosulfate is enhanced in the presence of mitochondrial ETC inhibitors.

关键词： Colorectal cancer Sodium thiosulfate HT-29 IEC6 Mitochondria Rotenone Clonogenic assay

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Progresses of mycobacteriophage-based Mycobacterium tuberculosis detection

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BIOCELL 2020年第4期44卷 683-694页

作者： ZICHEN LIU SIYAO GUO MENGZHI JI KAILI SUN ZHONGFANG LI XIANGYU FAN School of Biological Science and Technology University of JinanJinan250022China Nanyang Technological University Food Technology Centre(NAFTEC) 637459Singapore School of Chemical and Biomedical Engineering Nanyang Technological University637459Singapore College of Food and Bioengineering Hezhou UniversityHezhou542899China

Tuberculosis(TB)remains a major cause of morbidity and mortality worldwide,particularly in developing countries.A rapid and efficient method for TB diagnosis is indispensable to check the trend of tuberculosis *** emergence of drug-resistant bacteria has increased the challenge of rapid drug resistance *** to its high specificity and sensitivity,bacteriophage-based diagnosis is intensively *** this review,we mainly described mycobacteriophage-based diagnosis in TB detection,especially two prevalent approaches:fluorescent reporter phage and phage amplified biologically assay(PhaB).The rationale of reporter phage is that phage carrying fluorescent genes can infect host bacteria *** amplified biological assay based on the principle that phages can infect the live Mycobacterium tuberculosis in the specimen under suitable conditions and produce *** phage-based diagnostic methods,such as a combination of the amplified biologically assay and nucleic acid amplification or lateral flow assays,are also actively *** review will help us improve the understanding of mycobacteriophages in TB detection and better promote the development of the rapid diagnosis of ***.

关键词： Mycobacteriophage Tuberculosis Diagnosis Reporter phage Phage amplified biologically assay

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In vitro evaluation of anti-acetylcholinesterase and free radical scavenging potential of leaf extracts of some selected medicinal plants

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Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 60-65页

作者： Annie Jessica Toppo Sheela Chandra Dhruv Jha Papiya Mitra Mazumder Department of Bio-Engineering Birla Institute of Technology Department of Pharmaceutical Sciences and Technology Birla Institute of Technology

Objective: To evaluate the phytochemical present in various solvent extracts from leaves of Ocimum sanctum(L.), Swertia chirayita(L.), Butea monosperma(Lam.) and Stevia rebaudiana(Bert.) as well as antioxidant and anticholinergic activities employing different in vitro models. Methods: Total phenol content of diethyl ether, chloroform and methanolic extracts obtained from leaves of different medicinal plants was determined by Folin-Ciocalteau's spectrophotometric method. Moreover, antioxidant and anticholinergic studies were conducted by four different in vitro methods which included diphenyl picrylhydrazyl radical scavenging, 2,2-azinobis(3-ethylbezoline-6-sulphonic acid), reducing activity by ferrous reduced antioxidant power and anti-acetylcholinesterase assay, in order to ensure pharmacological potential of the plants. Results: The methanolic leaf extract of Ocimum sanctum showed the highest total phenol content which was(21.13±1.04) GAE/g DW and antioxidant activities compared to other plants with the IC50 value of 40.43 μg/mL in diphenyl picrylhydrazyl radical scavenging assay and 53.5 μg/mL in 2,2-azinobis(3-ethylbezoline-6-sulphonic acid) assay as well as metal ion reduced by(78.22±0.38) TE/g DW in ferrous reduced antioxidant power assay. The inhibition percentage of the anti-acetylcholinesterase assay was(94.22±0.26)%. Conclusions: The results of our current study show that Ocimum sanctum leaf is the most significant source of phytochemicals that possesses antioxidant and anticholinergic properties. However, further investigation on isolation and characterization of active compound which is responsible for the pharmacological potential is needed.

关键词： DPPH assay Antioxidant Free radical Anti-acetylcholinesterase Phenolics content

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Antioxidant activity and free radical scavenging activities of Streptomyces *** MJM 10778

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 962-967页

作者： Dong-Ryung Lee Sung-Kwon Lee Bong-Keun Choi Jinhua Cheng Young-Sil Lee Seung Hwan Yang Joo-Won Suh Division of Bioscience and Bioinformatics Myongji UniversityYoungin-siGyeonggi-do 449728Korea Center for Neutraceutical and Pharmaceutical Materials Myongji UniversityYongin-siGyeonggi-do 449728Korea

Objective:To investigate the antioxidant activity of soil-borne ***:The total phenolic contents,the level of antioxidant potential by DPPH radical scavenging activity,MO scavenging activity,and ABTS radical scavenging activity in ethyl acelale extract were ***:The 16 S rDNA sequencing analysis revealed that Streptomyces *** MJM *** was isolated from Hambak ***,has 99.9% similarity to Streptomyces misionensis(***) NBRC *** physiological and the morphological test revealed that the strain MJM 10778 has different characteristics from the strain *** entire antioxidant assay with the ethyl acelale extract displayed good radical scavenging *** IC_(50) values of the strain MJM 10778 extract on DPPH,.*** ABTS radicals were identified to he 92.8 μg/mL,0.02 μg/ml,and 134.9 μg/mL,*** ethyl acetate extract of the strain MJM 10778 showed an 81.500% of cell viability at 100 μg/mL in Raw264.7cell viability ***:The results obtained suggesl that the ethyl acetate extract of Streptomyces *** MJM 10778 could be considered as a potential source of drug for the diseases that is caused by free radicals with its anti-oxidant activities and low cytotoxicity.

关键词： Streptomyces sp.strain MJM 10778 Antioxidant activity DPPH radical scavenging assay NO radical scavenging assay

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Nrf2-inducing and HMG-CoA reductase inhibitory activities of a polyphenol-rich fraction of Guazuma ulmifolia leaves

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Asian Pacific Journal of Tropical Biomedicine 2019年第9期9卷 389-396页

作者： Sulistiyani Syamsul Falah Wulan Tri Wahyuni Dimas Andrianto Arthur Ario Lelono Waras Nurcholis Valeri Mossine Mark Hannink Tropical Biopharmaca Research Center of Excellence IPB University(Bogor Agricultural University)BogorIndonesia Department of Biochemistry Faculty of Mathematics and Natural SciencesIPB University(Bogor Agricultural University)BogorIndonesia Department of Chemistry Faculty of Mathematics and Natural SciencesIPB University(Bogor Agricultural University)BogorIndonesia Chemistry Research Center Indonesian Institute of SciencesSerpongIndonesia Biochemistry Department Christopher S.Bond Life Sciences CenterUniversity of Missouri-ColumbiaColumbia-MOUSA

Objective: To fractionate and identify polyphenols from Guazuma ulmifolia Lam. leaves, and to explore their antioxidant, 5-hydroxy-3-methylglutaryl-coenzyme A(HMG-Co A) reductase inhibitory, and Nrf2 modulatory ***: The 1,1-diphenyl-2-picrylhydrazyl assay was used to evaluate the antioxidant activity of a polyphenolic fraction of the extract of Guazuma ulmifolia Lam. leaves. THP-1 gene reporter cell lines constructed with a transcriptional response element specific for Nrf2 and a minimal promoter for the firefly luciferase–green fluorescent protein transgene were used to determine the effect of the polyphenolic fraction on the Nrf2 signaling pathway. Furthermore, an assay of HMG-Co A reductase inhibitory activity was performed by using a commercial enzyme kit. Polyphenolic compounds were identified by liquid chromatographytandem mass ***: The polyphenolic fraction showed fairly strong antioxidant activity [IC50 =(14.90 ± 4.70) μg/m L] and inhibited HMG-Co A reductase activity by 69.10%, which was slightly lower than that by pravastatin(84.37%) and quercetin(84.25%). Additionally, the polyphenolic fraction activated the Nrf2 antioxidant signaling pathway at 500 μg/m L. Eleven subfractions resulting from the column chromatography separation of the polyphenolic fraction also showed relatively strong antioxidant activities(IC50: 17.46–217.14 μg/m L). The subfraction(F6) stimulated the Nrf2 signaling pathway and had HMG-Co A reductase inhibitory activity(65.43%). Moreover, the subfraction contained two main flavonoids: quercetin and ***: The polyphenolic fraction of Guazuma ulmifolia could induce antioxidant genes via the Nrf2/antioxidant regulatory elements pathway, and is a promising candidate for an inhibitor of HMG-Co A reductase.

关键词： Antioxidant signaling HMG-Co A reductase inhibitor Polyphenols Antioxidant-related transcription factor Nrf2 Reporter gene cell-based assay

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