Progresses of mycobacteriophage-based Mycobacterium tuberculosis detection

作     者：ZICHEN LIU SIYAO GUO MENGZHI JI KAILI SUN ZHONGFANG LI XIANGYU FAN 

作者机构：School of Biological Science and TechnologyUniversity of JinanJinan250022China Nanyang Technological University Food Technology Centre(NAFTEC)637459Singapore School of Chemical and Biomedical EngineeringNanyang Technological University637459Singapore College of Food and BioengineeringHezhou UniversityHezhou542899China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2020年第44卷第4期

页      面：683-694页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the National Natural Science Foundation of China(31600148,81660331) the Foundation of University of Jinan(XBS1519,XKY1633) the Shandong Excellent Young Scientist Award Fund(BS2014YY031) High Level Innovation Teams of Guangxi Colleges&Universities/Outstanding Scholars Program(Guijiaoren35) Visiting Scholar Research Program of University of Jinan 

主　　题：Mycobacteriophage Tuberculosis Diagnosis Reporter phage Phage amplified biologically assay 

摘      要：Tuberculosis(TB)remains a major cause of morbidity and mortality worldwide,particularly in developing countries.A rapid and efficient method for TB diagnosis is indispensable to check the trend of tuberculosis *** emergence of drug-resistant bacteria has increased the challenge of rapid drug resistance *** to its high specificity and sensitivity,bacteriophage-based diagnosis is intensively *** this review,we mainly described mycobacteriophage-based diagnosis in TB detection,especially two prevalent approaches:fluorescent reporter phage and phage amplified biologically assay(PhaB).The rationale of reporter phage is that phage carrying fluorescent genes can infect host bacteria *** amplified biological assay based on the principle that phages can infect the live Mycobacterium tuberculosis in the specimen under suitable conditions and produce *** phage-based diagnostic methods,such as a combination of the amplified biologically assay and nucleic acid amplification or lateral flow assays,are also actively *** review will help us improve the understanding of mycobacteriophages in TB detection and better promote the development of the rapid diagnosis of ***.