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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第5期19卷 390-399页

作者： Xing-ting LIU Xin LIN Yu-ling MI Wei-dong ZENG Cai-qiao ZHANG Department of Veterinary Medicine College of Animal Sciences Zhejiang University

A rapid decline in egg production of laying hens begins after 480 d of age. Such a rapid decrease results predominantly from the ovarian aging, accompanied by endocrine changes, decreased yolk synthesis and accumulation, and the reduction in follicles selected into the preovulatory hierarchy. In this study, hens at 90, 150, 280, and 580 d old （D90, D150, D280, and D580, respectively） were compared for yolk precursor formation in the liver to elucidate effects of aging on laying performance. The results showed that liver lipid synthesis increased remarkably in hens from D90 to D150, but decreased sharply at D580 as indicated by the changes in triglyceride （TG） levels. This result was consistent with the age-related changes of the laying performance. The levels of liver antioxidants and total antioxidant capacity decreased significantly in D580 hens and the methane dicarboxylic aldehyde in D580 hens was much higher than that at other stages. The serum 17β-estradiol level increased from D90 to D280, but decreased at D580 （P〈0.05）. The expression of estrogen receptor a and 13 mRNAs in the liver displayed similar changes to the serum 17β-estradiol in D580 hens. Expressions of the genes related to yolk precursor formation and enzymes responsible for fat acid synthesis were all decreased in D580 hens. These results indicated that decreased yolk precursor formation in the liver of the aged hens resulted from concomitant decreases of serum 17β-estradiol level, transcription levels of estrogen receptors and critical genes involved in yolk precursor synthesis, and liver antioxidant status.

关键词： Lipid metabolism Yolk precursor formation 17β-estradiol Antioxidant Hen

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Evaluation of Estrogenic Activity and Measurement of EDCs in Wastewater Treatment Plants

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Journal of Ocean University of China 2006年第4期5卷 351-356页

作者： B. C. Lee J. Y. Jung H. K. Kim Environment & Process Technology Division Korea Institute of Science and Technology Seoul 136-791 Korea Department of Environmental Engineering National Institute of Environment Research Gwangju 500-712 Korea

Correlations between estrogenic activity and DOC/UV260 ratio in wastewater treatment processes were investigated to propose a simple, reliable and comprehensive indicator for the presence of estrogenic substances. Contrary to this, when short-term bioassays such as the E-SCREEN, receptor binding and reporter gene expression assays are used for detecting estrogenic activity in the wastewater sample, they require a long time, at least a few days. The major factors contributing to the estrogenic activity were found to be 17β-estradiol （E2） and estrone （El）. A good relationship between the DOC/ UV260 ratio and the concentration of estrogens （El and E2） in the effluent of the activated sludge process was found： the E2 concentration increased as the DOC/UV260 ratio increased while the E1 concentration decreased. The relative estrogenic activity and DOC/UV260 ratio showed a good correlation （R^2 = 0.84） for all sewage samples except the ozonized samples in the sewage treatment plants. This study shows that the estrogenic compounds are hard to be mineralized by the conventional biological processes. Advanced oxidation processes are required to further remove estrogenic substances in the secondary effluent. By analysis of DOC and UV260, the estrogenic activity in the wastewater can be rapidly estimated.

关键词： activated sludge process estrogenic substances 17β-estradiol Estrone DOC/UV260

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Determination of the Occurrence and Elimination of Endocrine Disrupting Compounds (EDCs) in Municipal Wastewater Treatment Plants (WWTP)

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Computational Water, Energy, and Environmental Engineering 2014年第1期3卷 1-7页

作者： Claudia Lange Bertram Kuch Joerg W. Metzger Department of Hydrobiology and Analysis of Organic Trace Compounds Institute for Sanitary Engineering Water Quality and Solid Waste Management University of Stuttgart Stuttgart Germany

In recent years, many research and development activities have focussed on endocrine disrupting compounds (EDCs) in rivers, lakes and surface waters as the potential cause of reproductive disturbances in different aquatic organisms e.g. fish and mollusk. The effluent of wastewater treatment plants was identified as main source for EDCs entering the aquatic environment. The purpose of the present study was to determine the estrogenic activity of wastewater and the elimination efficiency of various WWTPs in the different purification steps using the E-screen assay, an in-vitro test system based on the increasing proliferation of human breast cancer cells (MCF-7) in response to the presence of hormonal active substances. In contrast to expensive and time-intensive targeted instrumental single substance analysis an effect-related biological testing provides a sum parameter for the entirety of compounds contributing to the total estrogenic activity (agonists and antagonists, anti-estrogenic and also toxic compounds) in concentration units of the reference substance 17b-estradiol. The current standard purification methods of biological wastewater treatment in particular the activated sludge process significantly reduce estrogenicity (average 95%). Sorption on activated carbon and subsequent precipitation leads to a further reduction of the overall estrogenic activity up to 99%.

关键词： 17β-estradiol E-Screen Assay Estrogens Micropollutants Water Quality

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Clinical efficacy of anti-Mullerian hormone inspection in supporting diagnosis for climacteric disorders

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Open Journal of Internal Medicine 2011年第3期1卷 93-98页

作者： Takao Namiki Haruka Kakikura Yukari Matsumoto Ueno Koichi Hiromi Sato Atsushi Chino Akito Hisanaga Akiyo Kaneko Toshiaki Kita Maki Kihara Makio Shozu Katsutoshi Terasawa 不详

The diagnosis of climacteric disorders in gynecology is performed on the basis of symptoms rather than hormonal levels. Generally, the follicle-stimulating hormone (FSH) level increases when the 17β-estradiol (E2) level decreases in menopause. However, the problem of these hormones should be determined by strict timing of sampling, and there are individual specificities of decreases of hormone levels. We considered that anti-Mullerian hormone (AMH) can be measured at any time of the menstrual cycle and that it shows ovary functional decline earlier than FSH/E2, and we examined whether AMH would possibly become a good index for climacteric disorders. The subjects were 163 healthy females and 21 patients with climacteric disorders between 20 and 59 years old. We examined AMH, FSH and E2 at the same time. It is understood that in healthy females, AMH decreases with age and a decline in ovary function occurs at a relatively early age. Patients visiting clinics for climacteric disorders often have normal-range serum FSH/E2 levels, and it is clear that these values could not serve as indices of menopause at these inspections. Upon measurement of AMH in patients with climacteric disorders, most showed less than normal range (< 14 pmol/L), suggesting a decline in ovarian function. In addition, AMH was low in females with climacteric disorders compared with those without them. In conclusion, AMH was suggested as an objective index for climacteric disorders and possibly as a new diagnostic marker.

关键词： Climacteric Syndrome Anti-Mullerian Hormone (AMH) Kupperman Index(KI) Follicle Stimulating Hormone (FSH) 17β-estradiol (E2)

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Removal of Steriod Hormones by Activated Carbon Adsorption—Kinetic and Thermodynamic Studies

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Journal of Environmental Protection 2012年第6期3卷 469-475页

作者： Augustine O. Ifelebuegu Department of Geography Environment and Society Coventry University Coventry UK

The mechanism, isotherms and kinetics of removal of two endocrine disrupting chemicals, 17β-estradiol (E2) and 17α-ethinyloestradiol (EE2) by activated carbon adsorption were investigated in an agitated non-flow batch adsorption studies. Mathematical models were used to describe the adsorption phenomenon with the kinetic and thermodynamic parameters evaluated using the adsorption equilibrium data at varying temperatures. Higher adsorption rates were achieved at acidic to neutral pH ranges, with the sorption kinetic data showing a good fit to the pseudo second order rate equation and the Langmuir adsorption isotherm model for both E2 and EE2. The Gibbs free energy were –16.68 kJ/mol and –17.34 kJ/mol for E2 and EE2 respectively. The values of enthalpy for both E2 (84.50 kJ/mol) and EE2 (90 kJ/mol) indicated a chemical nature of the sorption process. Both the isotherm and thermodynamic data obtained all supported the mechanism of adsorption of E2 and EE2 to be mainly chemisorptions supported by some physical attractions.

关键词： Adsorption Isotherms 17β-estradiol 17α-Ethinyloestradiol Enthalpy

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How to explore the effects of sex hormone on blood vessels

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生殖医学杂志 2004年第Z1期13卷 75-78页

作者： Sun Mei-li Nie Min Liu Bing Du Zhi-jun Ge Qin-sheng Peking Union Medical College Hospital CAMS and PUMCBejing 100730 China Peking Union Medical College HospitalCAMS and PUMCBejing 100730 China Peking Union Medical College HospitalCAMS and PUMCBejing 100730 China Peking Union Medical College HospitalCAMS and PUMCBejing 100730 China Peking Union Medical College HospitalCAMS and PUMCBejing 100730 China

The risk of cardiovascular disease increases along with aging. There are increasing interests in researches on the protective effects of hormone replacement therapy (HRT)on cardiovascular system in postmenopausal women. In this article we will review how we have explored the evidence of different sex hormones on blood vessels since 1996. The results showed that low-dose HRT significantly protected cardiovascular system in postmenopausal ***, the high-dose 17β-estradiol (E2), with or without progesterone (P) or testosterone (T), or any hormone alone, as their concentrations increase, may even inhibit the protective effects of low-dose HRT on blood vessels.

关键词： Hormone replacement therapy 17β-estradiol Progesterone Testosterone Tissue factor Postmenopausal women

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Estrogen enhances ER-negative breast cancer cell motility and invasion:role of GPR30

Estrogen enhances ER-negative breast cancer cell motility an...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：王庭槐周科文孙鹏张亚星游昕超李平中山大学中山医学院心血管生理和生物反馈研究室

ER-negative breast cancer is an aggressive breast cancer ***,factors that involve in regulating its progression are not fully ***,we showed that the sex hormone17β-estradiol（E2）promoted ER-negative SK-BR-3 and MDA-MB-231 breast cancer cell migration and invasion in a dose dependent ***,E2 was able to activate the actin binding protein ezrin result in the formation of membrane protrusion in both of the cell ***-regulated GPR30expression by transfecting with a GPR30 si RNA largely attenuated the pro-migration and invasion effects of E2 in SK-BR-3 ***,while blockade GPR30 signaling cannot abrogate the effects of E2 in MDA-MB-231 cells,pre-incubated with an ERβantibody peptide or stimulated ERβsignaling with an agonist,DPN can attenuate or mimic the effects of E2,*** addition,co-incubated DPN with the GPR30 agonists,G1 or 4-OHT both down-regulated ERβprotein and phosphorylated AKT levels,indicating an antagonist effect between GPR30 and ERβsignaling in MDA-MB-231 *** together,our study provides the evidence that estrogen involves in influencing the progression of some ER-negative breast cancers that expressed ***,estrogen through GPR30 may exert distinct effects dependent on the ratio between GPR30 and ERβin target ER-negative cells.

关键词： GPR30 17β-estradiol ER-negative breast cancers

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17β-雌二醇心血管作用及其机制的研究

17β-雌二醇心血管作用及其机制的研究

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作者：刘文华南京医科大学

学位级别：硕士

Backgroud:Estrogen has extensive biological effect including against hypertension, atherosclerosis, lipid peroxidation and vascular injury, which play a pivotal role in cardiovascular protection. Vascular endothelial disfunction and myocardial metabolic disorder have been implicated in the pathogenesis of cardiovascular disease(CVD). Menopause means ovarian function failure and downstream estrogen levels. Premenopausal women are less risk of cardiovascular disease than men, the risk increase after menopause, similar to male. The International Menopause Society (IMS) in 2013 pointed out that perimenopausal and postmenopausal women with utilization of menopause hormone therapy (MHT) early can improve vessel function, lower cholesterol levels, regulate glycometabolism and blood pressure, which has potential cardiovascular protective effect, but the specific mechanism is unclear. Chronic inflammation and oxidative stress, is a central pathology physiology of the cardiovascular disease. Monocyte chemotactic factor 1 (MCP-1) inducts, regulates other formation and release of inflammatory factor, the formation of cascade, mediating inflammation, therefore it is considered to be the start of the inflammatory response factor. Induction of MCP-1, monocyte/macrophage activation and the accumulation of the subcutaneous is a key factor of atherosclerosis. RhoA belongs to Rho guanosine triphosphatase family, participating in maintaining vascular endothelium and the myocardial cytoskeleton structure, which plays an important role in regulation of cell migration. In recent years, studies have shown that SIRT1 can inhibit oxidative stress, inflammation, apoptosis, promote glucolipid metabolism, cell DNA damage repair, atherosclerosis, myocardial ischemia, myocardial hypertrophy and pathological processes of cardiovascular disease. AMP activated protein kinase (AMPK) is a cellular energy switch, adjust the AMP/ATP ratio, promote the mitochondrial oxidative metabolism of the generation

关键词： 17β-estradiol SIRT1 AMPK MCP-1 RhoA Cardiovascular protective effects

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AQP2 is regulated by estradiol in human endometrium and involved in spheroids attachment in vitro

AQP2 is regulated by estradiol in human endometrium and invo...

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2016全国中西医结合妇产科研究进展学术研讨会暨2016年第一届江浙沪中西医结合妇产科高峰论坛

作者： Ronghuan He Yanjun Hu Xijing Chen Xiaoling Hu Women’s Hospital School of MedicineZhejiang University

Background:17β-estradiol(E2) and aquaporin 2(AQP2) are associated with endometrial receptivity.E2 can directly regulate AQP2 expression in endometrial cancer *** study aimed to assess the role of AQP2 in embryo ***:Normal endometrial samples were collected from women who attended the Zhejiang Women’s Hospital for in vitro fertilization and embryo transfer(IVF-ET) *** with endometrial abnormalities were *** were categorized as early-mid proliferative,late proliferative,early secretary,mid-secretary,and late secretary ***2 mRNA(qRT-PCR) and protein(western blot) expression in response to E2 was assessed in normal human *** effects of AQP2 on spheroid attachment were assessed by an in vitro co-culture assay siRNA against AQP2 Results:The highest levels of AQP2 were observed during the late proliferative and mid-secretory *** lowest levels of AQP2 were observed during the early proliferative and late secretory *** 10 and 10 M E2 for 24 h up-regulated AQP2 in cultured ***-down of AQP2 by siRNA significantly decreased JAr spheroids attachment,with or without E2(P<0.01).Conclusion:AQP2 expression in human endometrium is mediated by *** AQP2 level could be a potential cause for impaired uterine receptivity.

关键词： Endometrium Aquaporin 2 17β-estradiol implantation human

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17β-雌激素调控大鼠髓核细胞自噬在椎间盘退变中作用的研究

17β-雌激素调控大鼠髓核细胞自噬在椎间盘退变中作用的研究

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作者：敖鹏南昌大学

学位级别：硕士

目的:本实验旨在初步探讨在17β-estradiol处理下髓核细胞中自噬与凋亡的关系及二者在椎间盘退变中的作用。方法:(1)体外分离培养SD大鼠髓核细胞,倒置相差显微镜下观察细胞形态变化,甲苯胺蓝、阿利新蓝染色及II胶原蛋白免疫组化染色鉴... 详细信息

目的:本实验旨在初步探讨在17β-estradiol处理下髓核细胞中自噬与凋亡的关系及二者在椎间盘退变中的作用。方法:(1)体外分离培养SD大鼠髓核细胞,倒置相差显微镜下观察细胞形态变化,甲苯胺蓝、阿利新蓝染色及II胶原蛋白免疫组化染色鉴定原代培养的细胞类型。(2)予于血清剥夺12、24、48、72H来诱导髓核细胞凋亡和自噬,然后用MDC染色、Annexin V-FITC/PI荧光染色法和Western blotting方法检测细胞凋亡、自噬和基质金属蛋白酶的表达水平变化,确立血清剥夺所致髓核细胞退变的最佳作用时间。(3)加入17β-estradiol并使其在培养液内的最终浓度分别为10-11、10-9、10-7M,随后使用Western blotting检测髓核细胞内自噬水平改变以及凋亡蛋白和基质金属蛋白酶的表达情况。用流式细胞仪检测不同浓度17β-estradiol作用下的髓核细胞的凋亡率变化情况。(4)应用自噬抑制剂预处理髓核细胞并通过流式细胞术、Western blotting方法、m Cherry-GFP-LC3-adenovirus转染观察17β-estradiol与血清剥夺诱导的髓核细胞自噬与凋亡之间的关系和可能存在的作用机制。结果:(1)分离培养的SD大鼠髓核细胞在倒置相差显微镜下观察大多呈多角形。正常髓核细胞大多数为圆形或椭圆形,可见多个核仁;经甲苯胺蓝染色后胞浆呈蓝色,细胞核呈紫蓝色;阿尔新蓝染色后细胞质呈淡蓝色;Ⅱ型胶原免疫细胞化学染色见细胞质黄色,细胞核周围散在棕黄色颗粒。(2)在血清剥夺处理下大鼠髓核细胞凋亡的表达水平明显高于对照组并呈现时间效应,自噬的表达水平也明显升高,但是在作用48h之后开始下降。(3)10-7 M 17β-estradiol能够显著提高血清剥夺48小时诱导的髓核细胞自噬并减少凋亡。(4)Western blot分析表明,当预先使用自噬抑制剂3-MA处理细胞1h后再加入17β-estradiol与细胞共同培养,发现Beclin-1和LC3-II/Ⅰ的表达被明显抑制,而蛋白cleaved caspase-3、MMP-3、MMP-13的表达却显著增加。流式细胞仪分析显示的结果与Western blot一致,加入3-MA后细胞凋亡率明显升高。荧光显微镜下计数m Cherry-GFP-LC3-adenovirus转染的自噬泡10-7 M的17β-estradiol组明显高于10-7 M的17β-estradiol联合3-MA组(*P17β-estradiol能抑制血清剥夺诱导的髓核细胞凋亡,并且抑制效果成剂量依赖性。(3)17β-estradiol能够通过上调自噬从而抑制血清剥夺诱导的髓核细胞凋亡;17β-estradiol上调自噬的同时也下调了MMP-3、MMP-13的表达水平。

关键词：髓核细胞饥饿椎间盘退变 17β-estradiol 自噬凋亡基质金属蛋白酶

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