AQP2 is regulated by estradiol in human endometrium and involved in spheroids attachment in vitro
作者单位：Women’s HospitalSchool of MedicineZhejiang University
会议名称：《2016全国中西医结合妇产科研究进展学术研讨会暨2016年第一届江浙沪中西医结合妇产科高峰论坛》
会议日期：2016年
学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Endometrium Aquaporin 2 17β-estradiol implantation human
摘 要：Background:17β-estradiol(E2) and aquaporin 2(AQP2) are associated with endometrial receptivity.E2 can directly regulate AQP2 expression in endometrial cancer *** study aimed to assess the role of AQP2 in embryo ***:Normal endometrial samples were collected from women who attended the Zhejiang Women’s Hospital for in vitro fertilization and embryo transfer(IVF-ET) *** with endometrial abnormalities were *** were categorized as early-mid proliferative,late proliferative,early secretary,mid-secretary,and late secretary ***2 mRNA(qRT-PCR) and protein(western blot) expression in response to E2 was assessed in normal human *** effects of AQP2 on spheroid attachment were assessed by an in vitro co-culture assay siRNA against AQP2 Results:The highest levels of AQP2 were observed during the late proliferative and mid-secretory *** lowest levels of AQP2 were observed during the early proliferative and late secretory *** 10 and 10 M E2 for 24 h up-regulated AQP2 in cultured ***-down of AQP2 by siRNA significantly decreased JAr spheroids attachment,with or without E2(P0.01).Conclusion:AQP2 expression in human endometrium is mediated by *** AQP2 level could be a potential cause for impaired uterine receptivity.