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Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair

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Bioactive Materials 2022年第5期7卷 15-31页

作者： Bi-Qin Lai Yu-Rong Bai Wei-Tao Han Bao Zhang Shu Liu Jia-Hui Sun Jia-Lin Liu Ge Li Xiang Zeng Ying Ding Yuan-Huan Ma Ling Zhang Zheng-Hong Chen Jun Wang Yuan Xiong Jin-Hua Wu Qi Quan Ling-Yan Xing Hong-Bo Zhang Yuan-Shan Zeng Key Laboratory for Stem Cells and Tissue Engineering(Sun Yat-sen University) Ministry of EducationGuangzhou510080China Department of Histology and Embryology Zhongshan School of MedicineSunYat-sen UniversityGuangzhou510080China Institute of Spinal Cord Injury Sun Yat-sen UniversityGuangzhou510120China Co-innovation Center of Neuroregeneration Nantong UniversityNantong226001China Department of Anatomy Anhui Medical UniversityHefei230032China Guangdong Provincial Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou510080China Department of Geriatrics The First Affiliated HospitalSun Yat-sen UniversityGuangzhou510080China Physiotherapy Department Guangdong Work Injury Rehabilitation HospitalGuangzhouChina Department of Orthopedic Surgery Key Laboratory of Musculoskeletal Trauma&War Injuries PLABeijing Key Lab of Regenerative Medicine in OrthopedicsThe 4th Medical CentreChinese PLA General HospitalBeijingChina

Directional axon regeneration and remyelination are crucial for repair of spinal cord injury(SCI),but existing treatments do not effectively promote those ***,we propose a strategy for construction of niche-specific spinal white matter-like tissue(WMLT)using decellularized optic nerve(DON)loaded with neurotrophin-3(NT-3)-overexpressing oligodendrocyte precursor cells.A rat model with a white matter defect in the dorsal spinal cord of the T10 segment was *** WMLT transplantation group showed significant improvement in coordinated motor functions compared with the control *** transplants integrated well with host spinal cord white matter,effectively addressing several barriers to directional axonal regeneration and myelination during SCI *** WMLT,laminin was found to promote development of oligodendroglial lineage(OL)cells by binding to laminin ***,laminin could also guide linear axon regeneration via interactions with specific integrins on the axon *** WMLT developed here utilizes the unique microstructure and bioactive matrix of DON to create a niche rich in laminin,NT-3 and OL cells to achieve significant structural repair of *** protocol can help to promote research on repair of nerve injury and construction of neural tissues and organoids that form specific cell niches.

关键词： Extracellular matrix Oligodendroglial lineage cells White matter-like tissue Directional axon regeneration Spinal cord injury

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整合素活化及其介导的黏着斑成熟与肿瘤转移

生理学报 2021年第2期73卷 151-159页

作者：黄梦汶林昶东陈剑峰细胞生物学国家重点实验室中国科学院分子细胞科学卓越创新中心中国科学院上海生物化学与细胞生物学研究所中国科学院大学上海200031 中国科学院大学杭州高等研究院生命与健康科学学院杭州310024

整合素是一类由α和β两个亚基组成的异源二聚体单次跨膜细胞黏附分子,通过与其对应配体相互作用,介导细胞与细胞、细胞与胞外基质之间的黏附,同时可以将细胞外信号传递至胞内,并招募一系列胞内蛋白与整合素胞内段结合,在细胞膜上形成... 详细信息

整合素是一类由α和β两个亚基组成的异源二聚体单次跨膜细胞黏附分子,通过与其对应配体相互作用,介导细胞与细胞、细胞与胞外基质之间的黏附,同时可以将细胞外信号传递至胞内,并招募一系列胞内蛋白与整合素胞内段结合,在细胞膜上形成超分子结构,激活下游信号。整合素的活化进程伴随着其胞外结构域由折叠构象转变为伸展构象以及胞内结构域的彼此分离。在细胞迁移过程中,整合素参与黏着斑的形成,连接胞外基质和细胞骨架,传递胞内-胞外的力学信号驱使细胞迁移。肿瘤微环境介导的整合素活化可促进多种类型细胞向肿瘤部位迁移,共同实现血管生成及肿瘤转移。本文对整合素的活化过程,其介导黏着斑动态变化引发的细胞迁移及对肿瘤转移的影响进行综述。

关键词：整合素活化黏着斑细胞迁移血管生成

Biochar-based slow-release of fertilizers for sustainable agriculture:A mini review

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Environmental Science and Ecotechnology 2022年第2期10卷 54-72页

作者： Chongqing Wang Dan Luo Xue Zhang Rong Huang Yijun Cao Gonggang Liu Yingshuang Zhang Hui Wang School of Chemical Engineering Zhengzhou UniversityZhengzhou450001China College of Materials Science and Engineering Central South University of Forestry and TechnologyChangsha410004China School of Chemistry and Chemical Engineering Central South UniversityChangsha410083HunanChina

Increasing global population and decreasing arable land pose tremendous pressures to agricultural *** application of conventional chemical fertilizers improves agricultural production,but causes serious environmental problems and significant economic *** gains increasing interest as a soil ***,more and more attentions have been paid to biochar-based slowrelease of fertilizers(SRFs)due to the unique properties of *** review summarizes recent advances in the development,synthesis,application,and tentative mechanism of biochar-based *** development mainly undergoes three stages:(i)soil amendment using biochar,(ii)interactions between nutrients and biochar,and(iii)biochar-based *** methods are proposed to improve the fertilizer efficiency of biochar,majorly including in-situ pyrolysis,co-pyrolysis,impregnation,encapsulation,and *** the distinct features of different methods,the integrated methods are promising for fabricating effective biochar-based *** in-depth understanding of the mechanism of nutrient loading and slow release is discussed based on current ***,the perspectives and challenges of the potential application of biochar-based SRFs are *** surveyed from this review indicates that applying biochar-based SRFs is a viable way of promoting sustainable agriculture.

关键词： Biochar Slow-release fertilizer Sustainability Soil amendment Agricultural production

Manipulation of Molecular Qubits by Isotope Effect on Spin Dynamics

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CCS Chemistry 2021年第9期3卷 2548-2556页

作者： Jing Li Shi-Jie Xiong Chun Li Biaobing Jin Yi-Quan Zhang Shang-Da Jiang Zhong-Wen Ouyang Zhenxing Wang Xing-Long Wu Johan van Tol You Song State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023 National Laboratory of Solid State Microstructures Department of PhysicsNanjing UniversityNanjing 210093 Research Institute of Superconductor Electronics(RISE) School of Electronic Science and EngineeringNanjing UniversityNanjing 210093 Jiangsu Key Laboratory for NSLSCS School of Physical Science and TechnologyNanjing Normal UniversityNanjing 210023 National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsBeijing Key Laboratory for Magnetoelectric Materials and DevicesCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871 Wuhan National High Magnetic Field Center&School of Physics Huazhong University of Science and TechnologyWuhan 430074 National High Magnetic Field Laboratory Florida State UniversityTallahasseeFL 32310

Controlling spin behavior via external stimuli is a key route to develop molecular spintronics ***,temperature,pressure,chemicals,and electric field are the possible ***,we report a new method,the isotope effect,to control spin behavior in molecule magnet *** can not only control the relaxation of magnetization,but also regulate the spin lifetime of quantum coherence.

关键词： molecule-based magnetism molecular qubits isotope effect spin–vibration coupling quantum coherence

Selective photocatalytic CO2 reduction over Zn-based layered double hydroxides containing tri or tetravalent metals

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Science Bulletin 2020年第12期65卷 987-994,M0003页

作者： Xuyang Xiong Yufei Zhao Run Shi Wenjin Yin Yunxuan Zhao Geoffrey I.N.Waterhouse Tierui Zhang Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academmy of SciencesBeijing WO 190China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China School of Physics and Electronic Science Hunan University of Science and TechnologyXiangtan 411201China School of Chemical Sciences The University of AucklandAuckland 1142New Zealand

Photocatalytic CO2 reduction holds promise as a future technology for the manufacture of fuels and commodity ***,factors controlling product selectivity remain poorly ***,we compared the performance of a homologous series of Zn-based layered double hydroxide(ZnM-LDH)photocatalysts for CO2 *** varying the trivalent or tetravalent metal cations in the ZnM-LDH photocatalysts(M=Ti4+,Fe3+,Co3+,Ga3+,Al3+),the product selectivity of the reaction could be precisely ***-LDH afforded CH4 as the main reduction product;ZnFe-LDH and ZnCo-LDH yielded H2 exclusively from water splitting;whilst ZnGa-LDH and ZnAl-LDH generated ***-situ diffuse reflectance infrared measurements,valence band XPS and density function theory calculations were applied to rationalize the CO2 reduction selectivities of the different ZnM-LDH *** analyses revealed that the d-band center(ed)position of the M3+or M4+cations controlled the adsorption strength of CO2 and thus the selectivity to carbon-containing products or *** with d-band centers relatively close to the Fermi level(Ti4+,Ga3+and Al3+)adsorbed CO2 strongly yielding CH4 or CO,whereas metal cations with d-band centers further from the Fermi level(Fe3+and Co3+)adsorbed CO2 poorly,thereby yielding H2 only(from water splitting).Our findings clarify the role of trivalent and tetravalent metal cations in LDH photocatalysts for the selective CO2 reduction,paving new ways for the development of improved LDH photocatalyst with high selectivities to specific products.

关键词： Photocatalytic CO2 reduction Layered double hydroxide Selectivity Tri/tetravalent metal cations d band center

Biosafety materials:Ushering in a new era of infectious disease diagnosis and treatment with the CRISPR/Cas system

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Biosafety and Health 2022年第2期4卷 70-78页

作者： Yuquan Zhang Ziyue Li Julien Milon Essola Kun Ge Xuyan Dai Huining He Haihua Xiao Yuhua Weng Yuanyu Huang School of Life Science School of Medical TechnologyAdvanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China Key Laboratory of Analytical Science and Technology of Hebei Province College of Chemistry and Environmental ScienceKey Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of EducationHebei UniversityBaoding 071000China Hunan Agricultural University Changsha 410128China Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics School of PharmacyTianjin Medical UniversityTianjin 300070China Beijing National Laboratory for Molecular Science State Key Laboratory of Polymer Physical and ChemistryInstitute of ChemistryChinese Academy of ScienceBeijing 100190China School of Materials and the Environment Beijing Institute of TechnologyZhuhai 519085China

Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on global *** clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system has the potential to address these critical needs for tackling infectious diseases to detect viral nucleic acids and inhibit viral *** review summarizes how the CRISPR/Cas system is being utilized for the treatment and diagnosis of infectious diseases with the help of biosafety materials and highlights the design principle and in vivo and in vitro efficacy of advanced biosafety materials used to deal with virus attacks.

关键词： Biosafety materials CRISPR/Cas SARS-CoV-2 Diagnosis Infectious disease

Effect of sex differences on prognosis of intravenous thrombolysis: data from the Thrombolysis Implementation and Monitor of Acute Ischemic Stroke in China (TIMS- China)

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Stroke & Vascular Neurology 2021年第1期6卷 10-15页

作者： Hongyu Zhou Weiqi Chen Yuesong Pan Yue Suo Xia Meng Hao Li Yongjun Wang Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina

Background and purpose Previous studies have reported conflicting results as to whether women have poorer functional outcome than men after thrombolytic *** study aims to investigate the relationship between sex differences and the prognosis of intravenous thrombolysis in Chinese patients with acute ischaemic *** The patients enrolled in this study were from the Chinese Acute Ischemic Stroke Thrombolysis Monitoring and Registration *** primary outcome was poor functional outcome,defined as a 3-month modified Rankin score of *** safe outcome was symptomatic intracranial haemorrhage(SICH)and mortality within 7 days and 90 *** Cox regression model was used to correct the potential covariates to evaluate the association between sex disparities and ***,the interaction of preonset Rankin scores,baseline National Institute of Health Stroke Scale(NIHSS)scores and Trial of Org 10172 in Acute Stroke Treatment(TOAST)types was statistically *** A total of 1440 patients were recruited,including 541 women and 899 *** baseline information indicated that women were older at the time of onset(66.2±11.2 years vs 61.0±11.3 years,p<0.001),and more likely to have a history of atrial fibrillation(25.3%vs 11.2%,p<0.001),and had a higher NIHSS score on admission(12.3±6.8 vs 11.6±6.7,p=0.04).According to the prognosis analysis of unsatisfactory functional recovery,there was no significant difference between women and men(45.9%vs 37.1%;adjusted OR 1.01,95%CI 0.75 to 1.37).As for the safe outcome,the proportion of SICH and mortality in women is relatively high but did not reach statistical *** was no significant interaction with sex,age,preonset Rankin score,NIHSS score,TOAST classification and the prognosis of intravenous *** For Chinese patients with ischaemic stroke,although women are older and more severe at the time of onset,the prognosis after intravenous thrombolysis is not si

关键词： thrombolysis Acute prognosis

Identification of SARS-CoV-2-against aptamer with high neutralization activity by blocking the RBD domain of spike protein 1

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Signal Transduction and Targeted Therapy 2021年第7期6卷 1985-1988页

作者： Ge Yang Ziyue Li Irfan Mohammed Liping Zhao Wei Wei Haihua Xiao Weisheng Guo Yongxiang Zhao Feng Qu Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceInstitute of Engineering MedicineKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijingChina State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijingChina Beijing National Laboratory for Molecular Sciences State Key Laboratory of Polymer Physics and ChemistryInstitute of ChemistryChinese Academy of SciencesBeijingChina Translational Medicine Center Key Laboratory of Molecular Target&Clinical Pharmacology and the State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences&the Second Affiliated HospitalGuangzhou Medical UniversityGuangzhouChina National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy ResearchGuangxi Medical UniversityNanningGuangxiChina

Dear Editor,The ongoing outbreak of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)poses a great threat to the public health of people and the normal economic and social development around the *** of January 8,2021,more than 88 million people were infected with SARS-CoV-2,resulting in more than 1.9 million death.

关键词： death acute respiratory

Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression

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Science China(Life Sciences) 2022年第11期65卷 2269-2286页

作者： Qin Jin Xiaoyu Yang Shixue Gou Xiaoyi Liu Zhenpeng Zhuang Yanhui Liang Hui Shi Jiayuan Huang Han Wu Yu Zhao Zhen Ouyang Quanjun Zhang Zhaoming Liu Fangbing Chen Weikai Ge Jingke Xie Nan Li Chengdan Lai Xiaozhu Zhao Jiaowei Wang Meng Lian Lei Li Longquan Quan Yinghua Ye Liangxue Lai Kepin Wang CAS Key Laboratory of Regenerative Biology Joint School of Life SciencesGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou 510530China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine Centre for Regenerative Medicine and HealthHong Kong Institute of Science and InnovationGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Sanya institute of Swine resource Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesHainan Provincial Research Center of Laboratory AnimalsSanya 572000China Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China Guangdong Provincial Key Laboratory of Laboratory Animals Guangdong Laboratory Animals Monitoring InstituteGuangzhou 510633China Guangdong Provincial Key Laboratory of Large Animal models for Biomedicine Wuyi UniversityJiangmen 529020China

Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological *** Tet-On system has been widely employed to regulate transgenic expression by *** pig models with tetracycline regulatory elements were generated through random *** process often resulted in uncertain expression and unpredictable phenotypes,thus hindering their ***,by precise knock-in of binary Tet-On 3G elements into Rosa26 and Hipp11 locus,respectively,a double knock-in reporter pig model was *** characterized excellent properties of this system for controllable transgenic expression both in vitro and in *** att P sites were arranged to flank the td Tomato to switch reporter *** or multiple gene replacement was efficiently and faithfully achieved in fetal fibroblasts and nuclear transfer *** display the flexible application of this system,we generated a pig strain with Dox-inducing h KRASexpression through phiC31 integrase-mediated cassette *** eight months of Dox administration,squamous cell carcinoma developed in the nose,mouth,and scrotum,which indicated this pig strain could serve as an ideal large animal model to study ***,the established pig models with controllable and switchable transgene expression system will provide a facilitating platform for transgenic and biomedical research.

关键词： double knock-in Tet-On system phiC31-mediated cassette exchange pig model hKRASG12D-related tumorigenesis