检索结果-南通市图书馆

Surveillance of Chikungunya virus activity in some North-eastern states of India

Asian Pacific Journal of Tropical Medicine 2019年第1期12卷 19-25页

作者： Prafulla Dutta Siraj A.Khan Anil C.Phukan Sarat Hazarika Naba K.Hazarika Sumi Chetry Abdul M.Khan Harpreet Kaur ICMR-Regional medical research Center North Eastern Indira Gandhi Regional Institute of Health & medical Sciences Jorhat medical College & Hospital Gauhati medical College & Hospital indian council of medical research

Objective: To detect the prevalence pattern of Chikungunya virus in three states of Northeast India. Methods: A total of 1 510 samples were collected from different private and government hospitals of Assam, Arunachal Pradesh and Meghalaya. Serum was tested for the presence of IgM antibodies against Chikungunya virus followed by RT-PCR for amplification of Chikungunya E1 gene region using specific primers. Results: Overall, 11.83%(172/1 454) clinical samples were positive by MAC-ELISA and/or RT-PCR assay. Asymptomatic infection was seen in 17.86%. Males were more affected than females and age group 16-30 years was mostly affected. Fever(100.00%) was the primary symptom followed by headache(72.03%) and arthralgia(41.53%). Only 118 Chikungunya positive cases could be traced, of which 25.42% complained about sequelae of infection. In entomological investigation, Aedes aegypti was more predominant(92.10%) than Aedes albopictus(7.90%). No mosquito pools could be incriminated for Chikungunya virus. Conclusions: In this study, Chikungunya was observed to be prevalent in Assam, Arunachal Pradesh and Meghalaya. Though Chikungunya is a selflimiting infection, increasing morbidity by CHIKV infection is affecting social and economic status of individual. Thus, a community empowerment to effectively control mosquito population by employing different mosquito control measures along with personal protection is mandatory to tackle future outbreak of the disease.

关键词： Chikungunya Northeast India Aedes aegypti Aedes albopictus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular characterization of DENV-3 circulating during the post-monsoon period of 2013–14 in Delhi, India

引用

Virologica Sinica 2015年第6期30卷 464-469页

作者： Pankaj Sharma Veena Mittal Mala Chhabra Roop Kumari Priyanka Singh Dipesh Bhattacharya Srinivas Venkatesh Arvind Rai Division of Zoonosis National Centre for Disease Control Centre for medical Entomology and Vector Management National Centre for Disease Control Division of Biotechnology National Centre for Disease Control

Dear Editor,Dengue infection is one of the emerging concerns for public health on a global *** the past few years,dengue transmission has increased in the Americas,the western Pacific and southeast *** magnitude,distribution,and clinical severity of dengue outbreaks have been an alarming signal in the southeast Asia *** outbreaks have been reported in countries in

关键词： southeast dengue India circulating monsoon in Delhi severity sequenced Major emerging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic diversity and gene structure of mitochondrial region of Anopheles minimus(Diptcra:Culicidae)-major malaria vector of North east India

引用

Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 952-955页

作者： Prafulla Dutta Siraj Ahmed Khan Rashmee Topno Pritom Chowdhury Mayuri Baishya Purvita Chowdhury Jagadish Mahanta Regional medical research Centre ICMR(NE Region)DibrugarhAssamIndia

Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(***)(Diptera:Culicidae) species from two malaria endemic states of NE ***:Phylogeographic analysis was carried at 9 out of 12 sites oi *** confirmed malaria endemic ***:All sequences were Adenine-Thymine rich *** were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 ease non synonymous mutation was *** distinct clusters of haplolypes were *** diversity and low nucleotide diversity were *** negative values obtained from Tajima's D test and Fu'sF_S test indicate a recent genetic population *** analysis has explained sequence diversity that was also shown by mutations in 6 ***:High genetic diversity observed within the populations of *** species has several possible implications for vector control in the region.

关键词： Anopheles minimus Cytochrome oxidase Ⅱ Haplotype Mutation Substitution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of NFKB1 gene polymorphism (rs28362491) with levels of inflammatory biomarkers and susceptibility to diabetic nephropathy in Asian indians

引用

World Journal of Diabetes 2017年第2期8卷 66-73页

作者： Amar Gautam Stuti Gupta Mohit Mehndiratta Mohini Sharma Kalpana Singh Om P Kalra Sunil Agarwal Jasvinder K Gambhir Department of Medicine University College of Medical Sciences (University of Delhi) and G.T.B. Hospital Molecular Diagnostic Laboratory Department of Biochemistry University College of Medical Sciences (University of Delhi) and G.T.B. Hospital

AIM To investigate the association of NFKB1 gene-94 ATTG insertion/deletion(rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian *** A total of 300 subjects were recruited(100 each), normoglycemic,(NG); type 2 diabetes mellitus(T2DM) without any complications(DM) and T2 DM with diabetic nephropathy [DM-chronic renal disease(CRD)]. Analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism and ELISA. Pearson's correlation, analysis of variance and logistic regression wereused for statistical *** The allelic frequencies of-94 ATTG insertion/deletion were 0.655/0.345(NG), 0.62/0.38(DM) and 0.775/0.225(DM-CRD). The-94 ATTG ins allele was associated with significantly increased levels of urinary monocyte chemoattractant protein-1(u MCP-1); u MCP-1(P = 0.026) and plasma tumor necrosis factor-alpha(TNF-α); TNF-α(P = 0.030) and almost doubled the risk of diabetic nephropathy(OR = 1.91, 95%CI: 1.080-3.386, P = 0.025).CONCLUSION-94 ATTG ins/ins polymorphism might be associated with increased risk of developing nephropathy in Asian indian subjects with diabetes mellitus.

关键词：糖尿病的 nephropathy 发炎 NFKB1 -94 ATTG ins/del 多型性尿单核白血球 chemoattractant protein-1 肿瘤坏死 factor-alpha

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sequence analysis of VP4 genes of wild type and culture adapted human rotavirus G1P[8]strains

引用

Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 541-546页

作者： Ritu Arora Ganesh S Dhale Pooja R Patil Shobha D Chitambar Enteric Viruses Group National Institute of VirologyPune India

Objective:To conduct a comparative analysis of the VP4 gene sequences of indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus ***:Full-length VP4 genes of each of the four wild type G1P[8]rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected to RT-PCR amplification and nucleotide sequencing. Results:All four cell culture adapted G1P[8]rotavirus strains showed nucleotide and amino acid substitutions in the VP4 gene as compared to their wild type *** number of substitutions however,varied from 1-64 and 1-13 *** substitutions were distributed in both VP5* and VP8* subunits of VP4 gene respectively of permeabilizalion and hemagglutinaling activity. The presence of unique amino acid substitutions was identified in two of the four wild type(V377G. S387N in 061060 and 1644L in 0715880) and all four cell culture adapted(A46V in 0613158-CA. T60R in 06361-CA,L237V.G389V and Q480H in 061060-CA and S615G and T625P in 0715880-CA) strains for the first time in the VP4 gene of P[8]*** acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type ***:Amino acid substitutions detected in the VP4 genes of G1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation,immunogenicity and conformation is needed for the development of newer rolavirus vaccines.

关键词： Rotavirus Cell culture G1P VP4 gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Thiamine deficiency perturbed energy metabolism enzymes in brain mitochondrial fraction of Swiss mice

引用

Frontiers in Biology 2017年第4期12卷 290-297页

作者： Anupama Sharma Renu Bist Surender Singh Department of Bioscience and Biotechnology Banasthali University BanasthaIL Rajasthan lndia Animal Biotechnology Centre National Dairy Research Institute Karnal India

BACKGROUND： Thiamine is an essential cofactor associated with several enzymes in energy metabolism and its deficiency may lead to neurological deficits. Current research evaluated the biochemical and molecular changes in TCA cycle enzymes using the mitochondrial fraction of the brain following thiamine deficiency （TD） in mice. METHODS： The investigation was carried out on Swiss mice （6-8 week old） allocated into three groups. First group was control; second and third group were made thiamine deficient for 8 and 10 days. RESULTS： Current study showed that alpha-ketoglutarate dehydrogenase （KGDHC）（thiamine-dependent enzyme） level found to be significantly reduced in experimental groups as compared to control group. In comparison to control group, a significant decrease in the succinate dehydrogenase （SDH） activity was calculated in group II and group III （p 〈 0.0001） mice. Diminished enzymatic activity of fumarase and MDH enzyme in thiamine deficient groups exposed for 8 and 10 days was calculated as compared to control group. The expression analysis of different genes governing TCA cycle enzymes in different experimental groups showed that there was a negotiable change in the expression of fumarase and DLD （dihydrolipoyl dehydrogenase- E3 subunit of KGDHC） whereas a declined in the expression of SDH and two subunits of KGDHC i.e. OGDH （2-oxoglutarate dehydrogenase- E1 subunit of KGDHC） and DLST （dihydrolipoyllysine-residue succinyltransferase- E2 subunit of KGDHC） was observed as compared to control group. CONCLUSIONS： Hence, current findings strongly entail that TD promotes alteration in energy metabolism in brain mitochondria which will decline the neuronal progression which may lead to neurodegenerative diseases such as Alzheimer＇s diseases.

关键词： thiamine brain TCA cycle enzymes mitochondrial dysfunction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular characterization of VP4,VP6,VP7 and NSP4 genes of group B rotavirus strains from outbreaks of gastroenteritis

引用

Asian Pacific Journal of Tropical Medicine 2011年第11期4卷 846-849页

作者： Anismrita Lahon Shobha D Chitambar Enteric Viruses Group National Institute of VirologyPune-411001India

Objective:To characterize VP4,VP6,VP7 and NSP4 genes of representative GBR strains(NIV- ***-04622 and NIV-094456) delected as the major eliolngic agenl in the outbreaks of gastroenteritis in western ***:Fecal specimens collected during the outbreaks of gastroenteritis were processed for RNA ***-PCR using GBR ***7 and NSP4 gene specific primers,nucleotide sequencing of the amplicons and phylogenetic analysis of the ***:Phylogenetic analysis of all of the ***7 and NSP4 gene sequences revealed clustering of GBR strains in indian-Bangladeshi lineage of genotype G2 with 95.8%- 99.4%nucleotide and 97.3%-100.0%amino acid ***,all three strains showed the presence of unique amino acid substitutions in the VP4 protein suggesting alteration in the antigenicity of outbreak strains of *** VP8* and VP5* regions of VP4 proteins showed respectively 0.5%-6.3%and 0.2%-1.1%amino acid divergence from human GBR strains of indian-Bangladeshi ***:These data confirm the reported variability of VP8* region and suggest the possible role of this region in the perpetuation of GBR infections in the *** is the first study to document the phylogenetic relationship of VP4,***7 and NSP4 genes of GBR strains detected in the outbreaks of gastroenteritis from India with the CBR strains from other parts of world.

关键词： Acute gastroenteritis Group B rotavirus VP4 VP6 VP7 NSP4

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Faunal richness and checklist of sandflies (Diptera: Pyschodidae) in India

引用

Asian Pacific Journal of Tropical Medicine 2023年第5期16卷 193-203页

作者： Harish Kumar Shah Fathima P A N Pradeep Kumar Ashwani Kumar Prasanta Saini ICMR-Vector Control research Centre(Field Station) KottayamKeralaIndia

This review aims to fill the voids and to update the checklist of sandfly fauna along with its spatial distribution in *** databases *** online or offline were searched to deduce the information to systematize the indian sandfly ***/data retrieved were screened and analysed to further update the available latest *** species name and authorship were given in accord with the International Code for Zoological *** compiled an updated checklist of reported indian sandfly species along with their state-wise distribution till 2022 as per published *** has maximum number of species reports when compared to other endemic states and states with pockets of *** argentipes is the most widely distributed,recorded so far,followed by other Sergentomiya and Phlebotomus species in *** this review,we have also described the vector and non-vector species of sandfly prevalent in different parts of the *** eoindianensis,an amber fossil sandfly species recorded from Gujarat might be older than other records of indian sandfly *** far 69 species(4 genera and 15 subgenera/groups)of Phlebotomine sandflies have been recorded in *** knowledge of species diversity and its distribution is a prerequisite for planning a systematic vector control strategy and disease management.

关键词： indian sandfly fauna Checklist Phlebotomine sandflies

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gymnema montanum improves endothelial function via inhibition of endoplasmic reticulum stress by activating Nrf2 signaling

引用

Asian Pacific Journal of Tropical Biomedicine 2020年第8期10卷 379-386页

作者： Dornadula Sireesh Natarajan Suganya Suvro Chatterjee Kunka Mohanram Ramkumar SRM research Institute SRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia Department of Biotechnology School of BioengineeringSRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia Vascular Biology Lab AU-KBC Research CentreAnna UniversityChromepetChennai-600044Tamil NaduIndia

Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial ***:The immortalized endothelial hybrid cell,***926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,*** cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were *** levels of ER stress markers,GRP78 and CHOP were analysed by Western blot *** Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter *** levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex ***:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced *** montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial *** also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial ***,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial ***:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.

关键词： ER stress Gymnema montanum Nrf2 Endothelial cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Monocyte chemoattractant protein-1, transforming growth factor-β1, nerve growth factor, resistin and hyaluronic acid as serum markers：comparison between recurrent acute and chronic pancreatitis

引用

Hepatobiliary & Pancreatic Diseases International 2016年第2期15卷 209-215页

作者： M Ganesh Kamath C Ganesh Pai Asha Kamath Annamma Kurien Department of Physiology Melaka Manipal Medical CollegeManipal UniversityManipalIndia Department of Pathology Melaka Manipal Medical CollegeManipal UniversityManipalIndia Department of Gastroenterology and Hepatology Kasturba Medical CollegeManipal UniversityManipalIndia Department of Community Medicine Kasturba Medical CollegeManipal UniversityManipalIndia

BACKGROUND： Diagnostic parameters that can predict the presence of chronic pancreatitis（CP） in patients with recurrent pain due to pancreatitis would help to direct appropriate therapy. This study aimed to compare the serum levels of monocyte chemoattractant protein-1（MCP-1）, transforming growth factor-β1（TGF-β1）, nerve growth factor（NGF）, resistin and hyaluronic acid（HA） in patients with recurrent acute pancreatitis（RAP） and CP to assess their ability to differentiate the two ***： Levels of serum markers assessed by enzymelinked immunosorbent assay（ELISA） were prospectively compared in consecutive patients with RAP, CP and in controls and stepwise discriminant analysis was performed to identify the markers differentiating RAP from ***： One hundred and thirteen consecutive patients（RAP=32, CP=81） and 78 healthy controls were prospectively enrolled. The mean（SD） age of the patients was 32.0（14.0）years; 89（78.8%） were male. All markers were significantly higher in CP patients than in the controls（P〈0.001）; MCP-1NGF and HA were significantly higher in RAP patients than in the controls（P〈0.001）. Stepwise discriminant analysis showed significant difference（P=0.002） between RAP and CP for resistin with an accuracy of 61.9%, discriminant scores of ≤-0.479 and ≥0.189 indicating RAP and CP, respectively. The other markers had no differential value between RAP and ***： Serum resistin is a promising marker to differentiate between RAP and CP and needs validation in future studies, especially in those with early CP.

关键词： biological marker cytokine nerve growth factor transforming growth factor pancreatitis resistin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：