Gymnema montanum improves endothelial function via inhibition of endoplasmic reticulum stress by activating Nrf2 signaling
作者机构：SRM Research InstituteSRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia Department of BiotechnologySchool of BioengineeringSRM Institute of Science and TechnologyKattankulathur-603203Tamil NaduIndia Vascular Biology LabAU-KBC Research CentreAnna UniversityChromepetChennai-600044Tamil NaduIndia
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2020年第10卷第8期
页 面：379-386页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the Indian Council of Medical Research(grant no.59/57/2011/BMS/TRM) Government of India
主 题：ER stress Gymnema montanum Nrf2 Endothelial cells
摘 要：Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial ***:The immortalized endothelial hybrid cell,***926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,*** cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were *** levels of ER stress markers,GRP78 and CHOP were analysed by Western blot *** Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter *** levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex ***:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced *** montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial *** also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial ***,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial ***:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.
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