Molecular characterization of VP4,VP6,VP7 and NSP4 genes of group B rotavirus strains from outbreaks of gastroenteritis

作     者：Anismrita Lahon Shobha D Chitambar 

作者机构：Enteric Viruses GroupNational Institute of VirologyPune-411001India 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第11期

页      面：846-849页

核心收录：

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 10[医学] 

基　　金：Indian Council of Medical Research(ICMR) New Delhi supported Anismrila Lahon with Junior Research Fellowship 

主　　题：Acute gastroenteritis Group B rotavirus VP4 VP6 VP7 NSP4 

摘      要：Objective:To characterize VP4,VP6,VP7 and NSP4 genes of representative GBR strains(NIV- ***-04622 and NIV-094456) delected as the major eliolngic agenl in the outbreaks of gastroenteritis in western ***:Fecal specimens collected during the outbreaks of gastroenteritis were processed for RNA ***-PCR using GBR ***7 and NSP4 gene specific primers,nucleotide sequencing of the amplicons and phylogenetic analysis of the ***:Phylogenetic analysis of all of the ***7 and NSP4 gene sequences revealed clustering of GBR strains in Indian-Bangladeshi lineage of genotype G2 with 95.8%- 99.4%nucleotide and 97.3%-100.0%amino acid ***,all three strains showed the presence of unique amino acid substitutions in the VP4 protein suggesting alteration in the antigenicity of outbreak strains of *** VP8* and VP5* regions of VP4 proteins showed respectively 0.5%-6.3%and 0.2%-1.1%amino acid divergence from human GBR strains of Indian-Bangladeshi ***:These data confirm the reported variability of VP8* region and suggest the possible role of this region in the perpetuation of GBR infections in the *** is the first study to document the phylogenetic relationship of VP4,***7 and NSP4 genes of GBR strains detected in the outbreaks of gastroenteritis from India with the CBR strains from other parts of world.