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Acellular scaffold‑based approach for in situ genetic engineering of host T‑cells in solid tumor immunotherapy

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Military Medical Research 2025年第1期12卷 16-35页

作者： Hiren Y.Dandia Mamatha M.Pillai Deepak Sharma Meghna Suvarna Neha Dalal Ayush Madhok Arvind Ingle Shubhada V.Chiplunkar Sanjeev Galande Prakriti Tayalia Department of Biosciences and bioengineering Indian Institute of Technology BombayPowaiMumbai 400076India Radiation Biology and Health Sciences Division Bhabha Atomic Research CentreMumbai 400085India Centre of Excellence in Epigenetics Department of BiologyIndian Institute of Science Education and ResearchPune 411008India Advanced Centre for Treatment Research and Education in Cancer(ACTREC)Tata Memorial CentreKhargharMumbai 410210India

Background:Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological ***,its application to solid tumors presents significant challenges due to the limited accessibility and *** delivery of tumor-specific T-cells using biomaterials has shown promise,however,procedures required for genetic modification and generation of a sufficient number of tumor-specific T-cells ex vivo remain major obstacles due to cost and time ***:Polyethylene glycol(PEG)-based three-dimensional(3D)scaffolds were developed and conjugated with positively charged poly-L-lysine(PLL)using carbamide chemistry for efficient loading of lentiviruses(LVs)carrying tumor antigen-specific T-cell receptors(TCRs).The physical and biological properties of the scaffold were extensively ***,the scaffold loaded with OVA-TCR LVs was implanted in B16F10 cells expressing ovalbumin(B16-OVA)tumor model to evaluate the anti-tumor response and the presence of transduced ***:Our findings demonstrate that the scaffolds do not induce any systemic inflammation upon subcutaneous implantation and effectively recruit T-cells to the *** B16-OVA melanoma tumor-bearing mice,the scaffolds efficiently transduce host T-cells with OVA-specific *** genetically modified T-cells exhibit homing capability towards the tumor and secondary lymphoid organs,resulting in a significant reduction of tumor size and systemic increase in anti-tumor *** cell profiling revealed a significantly high percentage of transduced T-cells and a notable reduction in suppressor immune cells within the tumors of mice implanted with these ***:Our scaffold-based T-cell therapy presents an innovative in situ localized approach for programming T-cells to target solid *** approach offers a viable alternative to in vitro manipulation of T-cells,circumventing the need for large-scale in vitro generat

关键词： Polyethylene glycol diacrylate Poly-L-lysine Lentiviruses T-cell therapy B16F10-OVA melanoma

Effects of polydimethylsiloxane membrane surface treatments on human uterine smooth muscle cell strain response

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Bioactive Materials 2024年第2期32卷 415-426页

作者： Elizabeth E.Marr Brett C.Isenberg Joyce Y.Wong Boston University Division of Materials Science and EngineeringUnited States Charles Stark Draper Laboratory Bioengineering DivisionUnited States Boston University Department of Biomedical EngineeringUnited States

In the United States,1 in 10 infants are born *** majority of neonatal deaths and nearly a third of infant deaths are linked to preterm *** birth is initiated when the quiescent state of the uterus ends prematurely,leading to contractions and parturition beginning as early as 32 weeks,though the origins are not well *** enable research and discovery of therapeutics with potential to better address preterm birth,the capability to study isolated cell processes of pregnant uterine tissue in vitro is *** development of an in vitro model of the myometrium utilizing human uterine smooth muscle cells(uSMCs)responsible for contractions provides a methodology to examine cellular mechanisms of late-stage pregnancy potentially involved in preterm *** discuss culture of uSMCs on a flexible polydimethylsiloxane(PDMS)substrate functionalized with cationic poly-L-lysine(PLL),followed by extracellular matrix(ECM)protein *** work exploring uSMC behavior on PDMS substrates have utilized collagen-I coatings,however,we demonstrated the first exploration of human uSMC response to strain on fibronectin-coated flexible membranes,importantly reflecting the significant increase of fibronectin content found in the myometrial ECM during late-stage *** the model we developed,we conducted proof-of-concept studies to investigate the impact of substrate strain on uSMC cell morphology and gene *** was found that PLL and varied ECM protein coatings(collagen I,collagen III,and fibronectin)altered cell nuclei morphology and density on PDMS ***,varied strain rates applied to uSMC substrates significantly impacted uSMC gene expression of IL-6,a cytokine associated with instances of preterm *** results suggest that both surface and mechanical properties of in vitro systems impact primary human uSMC phenotype and offer uSMC culture methodologies that can be utilized to further the understanding of cellu

关键词： Uterine Myometrium Bioengineered in vitro models Strain Pregnancy Nuclei Interleukin 6 Preterm labor

A mixed culture of Propionibacterium freudenreichii and Lactiplantibacillus plantarum as antifungal biopreservatives in bakery product

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Food Bioscience 2022年第3期47卷 413-420页

作者： Qiong Ran Fan Yang Min Geng Lujie Qin Zhongyi Chang Hongliang Gao Deming Jiang Chunjing Zou Caifeng Jia School of Life Sciences East China Normal UniversityShanghai200241China

In this study,15 strains of Lactic acid bacteria(Allata et al.)and 6 strains of propionic acid bacteria(PAB)were firstly tested for their antifungal activity against three spoilage fungi,Aspergillus niger,Penicillium crustosum and Aspergillus *** strains of LAB and PAB were selected and assessed,alone or paired,for their abilities to inhibit the most resistant mold,Aspergillus niger.A mixed culture of *** D6 and *** L9 was found to be the most active and their optimal inoculum was 1×10^(8) and 1×10^(6) CFU/mL,***,the in situ antifungal effect of the mixed culture was evaluated against bakery product spoilage *** was found that the growth of airborne fungi was delayed for up to 7 *** cell number of *** L9 was slightly increased by the presence of *** *** of three main acids(propionic,lactic and acetic acid)produced by the protective cultures suggested that acetic acid was mainly responsible for the antifungal *** conclusion,the mixed culture of *** and *** may be exploited as biopreservatives in bakery products.

关键词： Lactic acid bacteria Propionibacterium freudenreichii Antifungal activity Bread

Removal of copper from nickel anode electrolyte through ion exchange

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中国有色金属学会会刊：英文版 2009年第1期19卷 253-258页

作者：陈爱良邱冠周赵中伟孙培梅余润兰 School of Minerals Processing and bioengineering Central South University School of Metallurgical Science and Engineering Central South University

An novel method for removal of copper from nickel anodic electrolyte through ion exchange was studied after cupric deoxidization. Orthogonal design experiments show the optimum conditions of deoxidizing cupric into Cu+ in the nickel electrolyte are the reductive agent dosage is 4.5 times as the theoretic dosage and reaction time is 0.5 h at 40 ℃ and pH 2.0. Ion exchange experiments show that the breakthrough capacity(Y) decreases with the increase of the linear flow rate(X): Y=1.559-0.194X+ 0.006 7X2. Breakthrough capacity increases with the increase of the ratio of height to radius(RRH). The higher the initial copper concentration, the less the breakthrough capacity(BC). SO42- and nickel concentration have no obvious change during the process of sorption, so it is not necessary to worry about the loss of nickel during the sorption process. Desorption experiments show that copper desorption from the resin is made perfectly with NaCl solution added with 4% (volume fraction) H2O2 (30%) and more than 100 g/L CuCl2 solution is achieved.

关键词：钢气-固反应热力学分析金属材料

MODELING MICROBUBBLE DYNAMICS IN BIOMEDICAL APPLICATIONS

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Journal of Hydrodynamics 2012年第2期24卷 169-183页

作者： CHAHINE Georges L. HSIAO Chao-Tsung Dynaflow Inc.JessupMaryland 20794USA

Controlling microbubble dynamics to produce desirable biomedical outcomes when and where necessary and avoid deleterious effects requires advanced knowledge, which can be achieved only through a combination of experimental and numerical/ analytical techniques. The present communication presents a multi-physics approach to study the dynamics combining viscous- inviscid effects, liquid and structure dynamics, and multi bubble interaction. While complex numerical tools are developed and used, the study aims at identifying the key parameters influencing the dynamics, which need to be included in simpler models.

关键词： microbubble biological contrast agents thick shell ultrasound

Polymer-based Nanogenerator for Biomedical Applications

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Chemical Research in Chinese Universities 2020年第1期36卷 41-54页

作者： LI Jun LONG Yin WANG Xudong Department of Materials Science and Engineering University of Wisconsin-MadisonMadisonWisconsin 53706USA

Polymeric devices are the workhorses of modern *** one of the cutting-edge technology leveraging polymeric materials,nanogenerator that could convert micro-/nano-scale mechanical energy into electri-city based on the mechanism of piezoelectricity and triboelectricity exhibited great promise for biomedical applications,owning to the simple configuration,high efficiency,decent electrical output,biomimetic property as well as excellent *** this manuscript,the recent representative developments of NGs in biomedical applications are ***,such as working mechanisms underneath different NG prototypes are discussed,which is followed by innovative strategies endowing NG with biomimetic mechanical *** attempts to implement NG in specific biomedical fields(e.g.,power source for implantable medical devices,therapeutic electric stimulator,etc.)are introduced and *** manuscript ends up with subsection summarizing existed challenges while providing potential solutions for future NG developments in biomedical engineering.

关键词： Nanogenerator Polymeric material Biomedical application

MXene‑Graphene Composites:A Perspective on Biomedical Potentials

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Nano-Micro Letters 2022年第8期14卷 112-123页

作者： Ebrahim Mostafavi Siavash Iravani Stanford Cardiovascular Institute Stanford University School of MedicineStanfordCA 94305USA Department of Medicine Stanford University School of MedicineStanfordCA 94305USA Faculty of Pharmacy and Pharmaceutical Sciences Isfahan University of Medical SciencesIsfahanIran

MXenes,transition metal carbides and nitrides with graphene-like structures,have received considerable attention since their first *** the other hand,Graphene has been extensively used in biomedical and medicinal *** and graphene,both as promising candidates of two-dimensional materials,have shown to possess high potential in future biomedical applications due to their unique physicochemical properties such as superior electrical conductivity,high biocompatibility,large surface area,optical and magnetic features,and extraordinary thermal and mechanical *** special structural,functional,and biological characteristics suggest that the hybrid/composite structure of MXene and graphene would be able to meet many unmet needs in different fields;particularly in medicine and biomedical engineering,where high-performance mechanical,electrical,thermal,magnetic,and optical requirements are ***,the hybridization and surface functionalization should be further explored to obtain biocompatible composites/platforms with unique physicochemical properties,high stability,and *** addition,toxicological and long-term biosafety assessments and clinical translation evaluations should be given high priority in *** very limited studies have revealed the excellent potentials of MXene/graphene in biomedicine,the next steps should be toward the extensive research and detailed analysis in optimizing the properties and improving their functionality with a clinical and industrial ***,different synthesis/fabrication methods and performances of MXene/graphene composites are discussed for potential biomedical *** potential toxicological effects of these composites on human cells and tissues are also covered,and future perspectives toward more successful translational applications are *** current state-of-the-art biotechnological advances in the use of MXene-Graphene composites,as wel

关键词： Graphene MXene Composites Hybrid structures Biocompatibility Cancer theranostics Biomedical engineering

Biochemical methane potential of three-phase olive mill solid waste:Influence of temperature and supplemental enzymes

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Carbon Resources Conversion 2022年第3期5卷 248-254页

作者： Rafat Al Afif Christoph Pfeifer Institute of Chemical and Energy Engineering Department of Material Sciences and Process EngineeringUniversity of Natural Resources and Life SciencesViennaMuthgasse 1071190 ViennaAustria

Methane potential of three-phase olive mill solid waste(3POMSW)was evaluated by biochemical methane potential(BMP)tests at temperatures of 37.5◦C and 55◦*** results have shown,that thermophilic processing has a higher methane and biogas yield by 10%and 17.2%,respectively,compared to mesophilic *** thermophilic conditions of 3POMSW results in higher conversion of the feedstock into *** result of addition of enzymes(MethaPlus,Hemicellulase,PectinaseL,Amylase S and Lipase)and their mixtures to 3POMSW at 55◦C has shown that the optimum mixture of enzymes was Metha Plus and Hemicellulase,which resulted in an increase of methane yield by 1.3%compared to the base case without addition of *** model equations were developed based on first-order kinetic in order to calculate the biogas yield versus the time as a function of reaction rate constant(k)and the maximum biogas yield(ymax).In this study,results have shown two different reaction rates obtained for the mixtures of enzymes with 3POMSW of 0.130 d^(-1) from t=0…14 days and 0.034 d^(-1) from t=28…63 *** developed simple model equations can be applied for calculating the biogas yield at the time and by optimizing the process improving biological nutrient removal.

关键词： Three-phase olive mill solid waste Biochemical methane potential Mesophilic Thermophilic Anaerobic digestion

A Novel Vision of Reinforcing Nanofibrous Masks with Metal Nanoparticles:Antiviral Mechanisms Investigation

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Advanced Fiber Materials 2023年第4期5卷 1273-1317页

作者： Farinaz Hadinejad Hamed Morad Mohsen Jahanshahi Ali Zarrabi Hamidreza Pazoki‑Toroudi Ebrahim Mostafavi Nanotechnology Research Institute Faculty of Chemical EngineeringBabol Noushirvani University of TechnologyBabol ***Iran Department of Pharmaceutics and Pharmaceutical Nanotechnology School of PharmacyIran University of Medical SciencesTehran ***Iran Ramsar Campus Mazandaran University of Medical SciencesRamsar ***Iran Department of Biomedical Engineering Faculty of Engineering and Natural SciencesIstinye UniversityIstanbul 34396Turkey Physiology Research Center Faculty of MedicineIran University of Medical SciencesTehran ***Iran Department of Physiology Faculty of MedicineIran University of Medical SciencesTehran ***Iran Stanford Cardiovascular Institute Stanford University School of MedicineStanfordCA 94305USA Department of Medicine Stanford University School of MedicineStanfordCA 94305USA

Prevention of spreading viral respiratory disease,especially in case of a pandemic such as coronavirus disease of 2019(COVID-19),has been proved impossible without considering obligatory face mask-wearing protocols for both healthy and contaminated *** widespread application of face masks for long hours and almost everywhere increases the risks of bacterial growth in the warm and humid environment inside the *** the other hand,in the absence of antiviral agents on the surface of the mask,the virus may have a chance to stay alive and be carried to different places or even put the wearers at risk of contamination when touching or disposing the *** this article,the antiviral activity and mechanism of action of some of the potent metal and metal oxide nanoparticles in the role of promising virucidal agents have been reviewed,and incorporation of them in an electrospun nanofibrous structure has been considered an applicable method for the fabrication of innovative respiratory protecting materials with upgraded safety levels.

关键词： Nanoshield Facemask Antiviral Metal nanoparticles Electrospinning Immunity booster