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Acta Biochimica et Biophysica Sinica 2007年第2期39卷 101-107页

作者： Yunhua HOU~(1,2) Tianhong WANG~1 Hao LONG~1 and Huiynan ZHU~(1,2) ~1State Key Laboratory of Microbial Technology,shandong University,Jinan 250100,china ~2Department of food and biologic engineering,shandong institute of light industry,Jinan 2.032.,china StateKeyLaboratoryofMicrobialTechnology ShandongUniversityJinan250100China Departmentoffoodandbiologicengineering ShandongInstituteofLightIndustryJinan250323China

A cellobiohydrolase 1 gene (cbhl) was cloned from Penicillium chrysogenum FS010 by amodified thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR).DNA sequencing showsthat cbhl has an open reading frame of 1590 bp, encoding a putative protein of 529 amino acid *** amino acid sequence revealed that CBHI has a modular structure with a predicted molecular massof 56 kDa and consists of a fungal type carbohydrate binding module separated from a catalytic domain bya threonine rich linker *** putative gene product is homologous to fungal cellobiohydrolases inFamily 7 of the glycosyl hydrolases.A novel cbhl promoter (1.3 kb) was also cloned and sequenced,whichcontains seven putative binding sites (5'-SYGGRG-3') for the carbon catabolite repressor *** ofvarious carbon sources to the cbhl transcription of *** was examined by Northern analysis,suggesting that the expression of cbhl is regulated at transcriptional *** cbhl gene in cold-adaptivefungus *** was expressed as an active enzyme in Saccharomyces cerevisiae *** recombinantCBHI accumulated intracellularly and could not be secreted into the medium.

关键词： Penicillum chrysogenum cellobiohydrolase TAIL-PCR promoter of cbh1

Israeli Water Achievements Today and Enlightenment to china

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Chinese Journal of Population,Resources and Environment 2008年第4期6卷 72-77页

作者： Sun Lei1,2, Xu Lanqing3 1. institute of Environmental Science and engineering, Ocean University of china, Qingdao shandong 266100, china 2. Department of Soil and Water Sciences, Faculty of Agricultural, food and Environmental Quality Sciences, the Hebrew University of Jerusalem, 76100, Israel 3. Logistics Group of Ocean University of china, Qingdao shandong 266100, china institute of Environmental Science and engineering Ocean University of China Qingdao Shandong 266100 China Department of Soil and Water Sciences Faculty of Agricultural Food and Environmental Quality Sciences the Hebrew University of Jerusalem 76100 Israel Logistics Group of Ocean University of china Qingdao Shandong 266100 China

Despite the endemic drought, arid climate and associated limited freshwater resources, as well as difficult geopolitical realities, Israel can provide a reliable supply of high quality water to a rapidly growing population. The achievements of water supply in Israel can be summarized as water technologies (like water transportation and purification, desalination, wastewater treatment, effluent reuse, storm water catchment, control systems, rain enhancement and drip irrigation, etc.), water management and legislation as well as water industry. In view of the national concern regarding the quality and quantity of the water, the skills, technologies and know-how that Israel has accumulated in water supply and irrigation can give us enlightenment that is also presented in this paper.

关键词： Israel china water technology water management water industry

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非水毛细管电泳对9种手性药物的拆分

色谱 2003年第2期21卷 138-142页

作者：初永宝蒋文强崔凤霞李关宾山东大学环境科学与工程学院山东济南250100 山东轻工业学院食品工程系山东济南250100

用非水毛细管电泳成功地分离了洛贝林、托吡卡胺、普萘洛尔、阿替洛尔、环扁桃酯、沙丁胺醇、苯海索、芬氟拉明和异丙嗪等9种手性化合物。以甲酰胺为介质、柠檬酸三羟甲基氨基甲烷(Tris)为电解质、在254nm波长下紫外检测。对手性选择... 详细信息

用非水毛细管电泳成功地分离了洛贝林、托吡卡胺、普萘洛尔、阿替洛尔、环扁桃酯、沙丁胺醇、苯海索、芬氟拉明和异丙嗪等9种手性化合物。以甲酰胺为介质、柠檬酸三羟甲基氨基甲烷(Tris)为电解质、在254nm波长下紫外检测。对手性选择剂的类型及浓度、背景电解质的pH值以及离子强度等因素对分离的影响做了系统的研究,最后确立了9种手性药物的最佳分离条件。实验发现,不同的手性选择剂对手性药物具有不同的分离选择性;每一种选择剂都存在一个相对水相体系较高的最佳浓度值而使分离效果最佳;在非水体系中,最佳pH值要比在水相体系中高;其分离效率也是随着体系中电泳介质离子强度的增大而升高。

关键词：手性药物非水毛细管电泳手性拆分甲酰胺分离

Differential gene expression of phosphoglyceric kinase (PGK) and hypoxic adaptation in chicken

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Science china(Life Sciences) 2007年第3期50卷 335-342页

作者： WANG CunFang1,3, YUAN CunZhong2, ZHANG Lao1, WU ChangXin1 & LI Ning2.1.College of Animal Science and Technology, china Agricultural University, Beijing 100094, china 2 china State Key Laboratories for Agrobiotechnology, china Agricultural University, Beijing 100094, china 3 College of food and biologic engineering, shandong institute of light industry, Jinan 250353, china 1. College of Animal Science and Technology China Agricultural University Beijing 100094 China 3. College of food and biologic engineering Shandong Institute of Light Industry Jinan 250353 China 2. china State Key Laboratories for Agrobiotechnology China Agricultural University Beijing 100094 China

Four single-nucleotide polymorphisms (SNP) of the Phosphoglyceric Kinase (PGK) gene were discov- ered based on comparison of the sequences from an altiplano chicken breed (Tibetan chicken) and two lowland breeds (White Leghorn and Shouguang chicken). Gel-shift results indicate that one of these SNPs, an A→G mutation at position 59 in exon10, is able to bind hypoxia-induced factor-l (HIF-1), functioning as a hypoxia response element (HRE). The mutant gene results in M→T mutation at position 379 amino acid. The combined activity of this HRE and HIF-1 could increase correspondingly under a hypoxic stimulus. Hypoxia leads to increased death rates of chicken embryos; while the M→T mutation described herein is prevalent in healthy embryos grown under hypoxic conditions, thus it may repre- sent an adaptation to hypoxia. Fluorescence quantitative reverse transcription PCR results revealed that HIF-1 upregulates the transcript level of the glycolytic enzyme PGK in the brain and skeletal mus- cle of animals subjected to hypoxia. Thus, a large amount of ATP is produced by increased glycolysis, allowing the organism to meet energy metabolism demands. As such, we believe this SNP to be an adaptation to the external anoxic environment.

关键词： gene and hypoxic adaptation in chicken Differential gene expression of phosphoglyceric kinase PGK SNPs HRE

谷氨酸生产菌S^(9114)中的谷氨酸脱氢酶的研究

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生物工程学报 2003年第6期19卷 725-729页

作者：王燕宋香杨平平段作营毛忠贵江南大学生物工程学院工业生物技术教育部重点实验室

谷氨酸脱氢酶 (GDH)是谷氨酸生物合成的关键酶 ,谷氨酸棒杆菌S91 1 4是目前我国味精工业应用最广泛的生产菌种 ,其谷氨酸脱氢酶的研究尚未见报道。分离纯化该菌中的谷氨酸脱氢酶 ,研究其辅酶组成 ,对揭示谷氨酸脱氢酶的分子结构和性质 ... 详细信息

谷氨酸脱氢酶 (GDH)是谷氨酸生物合成的关键酶 ,谷氨酸棒杆菌S91 1 4是目前我国味精工业应用最广泛的生产菌种 ,其谷氨酸脱氢酶的研究尚未见报道。分离纯化该菌中的谷氨酸脱氢酶 ,研究其辅酶组成 ,对揭示谷氨酸脱氢酶的分子结构和性质 ,提高谷氨酸产率很有必要。将培养至对数期中期的细胞离心收集并用含适量DTT、ED TA的Tris_HCl缓冲液 (pH 7 5 )洗涤 ,用Frenchpressurecellpress破碎 ,离心去除菌体碎片得无细胞抽提液。然后使用 KTA_10 0快速纯化系统经DEAE_纤维素柱、疏水柱 (HIC)、G_2 0 0凝胶过滤柱层析得到纯化大约 70倍的以NAD PH为辅酶的GDH和部分纯化的以NADH辅酶的GDH。这两个酶分别对NADPH、NADH高度专一 ,不能相互代替。经HPLC和SDS_PAGE测得前一种酶的分子量和亚基分子量分别为 188kD和 32kD ,表明该酶为具有相同亚基的六聚体。酶活性测定使用HITACHIU_30 0 0分光光度计利用NAD(P)H在 340nm氧化的初速度进行。蛋白质含量测定利用Bradford方法进行 ,并以牛血清白蛋白为标准蛋白。纯化结果表明S91 1 4中确实存在两种GDH ,其中以NADH为辅酶的GDH尚未见报道。和某些具有两种GDH的微生物一样 ,S91 1 4可能也是以NADPH为辅酶的GDH参与谷氨酸的合成代谢 ,以NADH为辅酶的GDH参与谷氨酸的分解代谢。

关键词：谷氨酸脱氢酶提纯谷氨酸棒杆菌 NADH NADPH 谷氨酸生产菌