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Thermal preconditioning protected cerebellar granule neurons of rats by modulating HSP70 expression

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Acta Pharmacologica Sinica 2004年第4期25卷 60-63页

作者： Li-jun CHEN, Xing-wen SU, Peng-xin QIU, Yi-junHUANG, Guang-mei YAN2 department of Pharmacology, zhongshan medicalcollege, sun yat-sen university, guangzhou 510089, china department of Pharmacology Zhongshan Medical College Sun YatSen University Guangzhou 510089

AIM: To explore the possibility that expressions of different heat shock proteins (HSPs) are specifically involved in the protection of thermal preconditioning against apoptosis of cerebellar granule neurons (CGNs) induced by repolarization. METHODS: Western blotting was used to detect expressions of HSP27, HSP70, and HSP90 in- duced by thermal preconditioning (TP) in CGNs; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of HSP70 mRNA; apoptosis of CGNs was induced by switching culture medium containing KCl 25 mmol/L to one containing KCl 5 mmol/L. RESULTS: No expression of HSP27 in cerebellar granule neurons was observed with TP at 44 oC. Expression of HSP90 was obvious in CGNs both without and with TP at 44 oC for different periods. Expression of HSP70 protein in CGNs was lower with TP at 44 oC for 5 min, but it increased gradually after the period was prolonged to 30, 60, or 90 min. HSP70 mRNA was detected after TP 44 oC for 30, 60, and 90 min and increased gradually with time. HSP70 antisense oligodeoxynucleotides 10 μmol/Lfor 72 h inhibited the protective effects of TP at 44 oC on apoptosis of CGNs induced by repolarization. CONCLUSION: HSP70 is involved in protective effects of thermal preconditioning on apoptosis in cerebellar granule neurons induced by repolarization.

关键词： thermal preconditioning heat heat shock proteins cerebellum neurons apoptosis antisense oligodeoxynucleotides Western blotting

Microtubule stability and MAP1B upregulation control neuritogenesis in CAD cells

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Acta Pharmacologica Sinica 2006年第9期27卷 1119-1126页

作者： Wen LI~(2,5)Jin-tang XIA~3 Yue FENG~(4,5)2 Laboratory of Surgery,The First Affiliated Hospital,sun yat-sen university,guangzhou 510080,china 3 department of General Surgery,The First People's Hospital of guangzhou,guangzhou 510180,china 4 department of Pharmacology,Emory university School of Medicine,USA Laboratory of Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou China department of General Surgery The First People's Hospital of Guangzhou Guangzhou China department of Pharmacology Emory University School of Medicine USA

Aim:To study the role of microtubule dynamics and microtubule associated pro-tein 1B(MAP1B)in regulation of the neurite extension in CAD catecholaminergicneuronal cell ***:The neuritogenesis of the CAD cells was abolishedby inhibiting microtubule polymerization with nocodazole and by blocking micro-tubule depolymerization with ***1B and tubulin protein expression wasdetected by Western *** staining of tubulins was observedby fluorescent and confocal ***:Microtubule dynamics wasessential for CAD neurite *** analysis revealed that neurite exten-sion was much more sensitive to nocodazole than to taxol,suggesting a func-tional requirement for highly active microtubule assembly.A remarkableupregulation of MAP IB protein was detected during neurite extension accompa-nied with increased microtubule ***:Upregulation of MAP1Bleads to the stabilization of newly formed microtubules in the developing neurites,which in turn promotes neurite extension.

关键词： microtubule stability microtubule associated protein 1B neuritogenesis

Biorthogonal multiple wavelets generated by vector refinement equation

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Science china Mathematics 2007年第7期50卷 1015-1025页

作者： Song LI~(1+) Jun XIAN~2 1 department of Mathematics,Zhejiang university,Hangzhou 310027,china 2 department of Mathematics,sun yat-sen university,guangzhou 510275,china department of Mathematics Zhejiang University Hangzhou China department of Mathematics Sun Yat-Sen University Guangzhou China

Biorthogonal multiple wavelets are generated from refinable function vectors by using the multiresolution analysis. In this paper we provide a general method for the construction of compactly supported biorthogonal multiple wavelets by refinable function vectors which are the solutions of vector refinement equations of the form $$\varphi (x) = \sum\limits_{\alpha \in \mathbb{Z}^s } {a(\alpha )\varphi (Mx - \alpha ), x \in \mathbb{R}^s } ,$$ where the vector of functions ? = (? 1, …, ? r)T is in $(L_2 (\mathbb{R}^s ))^r ,a = :(a(\alpha ))_{\alpha \in \mathbb{Z}^s } $ is a finitely supported sequence of r × r matrices called the refinement mask, and M is an s × s integer matrix such that lim n→∞ M ?n = 0. Our characterizations are in the general setting and the main results of this paper are the real extensions of some known results.

关键词： refinement equation biorthogonal multiple wavelets refinable function vector 42C40 41A10 41A15 41A25

OCULO-CEREBRO-RENAL SYNDROME (LOWE'S SYNDROME)

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眼科学报 1991年第1期7卷 40-42,46页

作者： Youzhao Chen zhongshan Ophthalmic Center sun yat-sen university of medical Sciences guangzhou, china Guoxiang Pang department of Ophthalmology Peking Union medical college Hospital Beijing, china zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

Oculo-cerebro-renal syndrome (Lowe's syndrome) is characterized by mental and motor retardation, cataract, glaucoma and renal abnormalities. It is an X-linked recessive metabolic disease. Two brothers suffering from Lowe's syndrome are reported. Their mother with lenticular opacities and peculiar facial appearance is in concordance with the obligate carrier. The ocular changes and heridity are discussed.

关键词： oculo-cerebro-renal syndrome Lowe’s syndrome congenital cataract carrier female X-R heredity metabolic abnomalities.

IMPROVED DEM-CFD MODEL AND VALIDATION:A CONICAL-BASE SPOUTED BED SIMULATION STUDY

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Journal of Hydrodynamics 2010年第3期22卷 351-359页

作者： RONG Liang-wan ZHAN Jie-min department of Applied Mechanics and Engineering Sun Yat-sen University Guangzhou 510275 China department of Applied Mechanics and Engineering Sun Yat-sen University Guangzhou 510275 China Guangdong Province Key Laboratory of Coastal Ocean Engineering Sun Yat-sen University Guangzhou 510275China

An improved and efficient DEM-CFD approach is developed for spouted beds. A nonlinear Discrete Element Method （DEM）, with a concept of spring, dash-pot and friction slider, is used for tracing the movement of each individual particle. The gas flow is described by a set of reorganized governing equations. Two phases are coupled through contributions due to effects of porosity, viscosity and drag. All equations are solved with the commercial package Fluent with an implementation of User Defined Functions （UDF）. To validate the improved model, a two-dimensional conical-base spouted bed is chosen as a case study. An unstructured mesh system is adopted instead of regular grid system. The simulation also takes the Saffman force and Magnus effect into account. The calculation results show good agreement with the experimental observations which are taken from the literature.

关键词： Discrete Element Method (DEM) spouted bed Fluent User Defined Functions (UDF) unstructured mesh

Experimental study of gene therapy with angiostatin gene in pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 452-457页

作者： Sheng-Li Nie Shi-Zhen Yuan the department of Gastroenterology, sun yat-sen Memorial Hospital, medical college, sun yat-sen university, guangzhou 510120, china department of Gastroenterology Sun Yat-Sen Memorial Hospital Sun Yat-Sen University Guangzhou 510120 China

Objective: To study the effects of angiostatin (AS) gene mediated by liposome on human pancreatic cancer cell line SW1990. Methods: Angiostatin gene was cloned into the eu- karyotic expression vector pRC/CMV. The recombi- nant of pRC/CMV-AS was introduced into the pan- creatic cancer cell line, SW1990. The mechanism of anti-tumor was studied and tested. Results: The eukaryotic expression vector pRC/ CMV-AS was identified by the restriction digest. pRC/CMV-AS was stably integrated into the target cells and expressed by Western blot and drug-sensi- tivity tests, and inhibited the vascular endothelial cells proliferation in vitro. In addition, the effects of the angiostatin vector on reducing the volume of tumors implanted in nude mouse models were also noted. Conclusion: This study demonstrated that the recom- binant pRC/CMV-AS mediated by liposome may play a potential role in the treatment of pancreatic cancer in the future.

关键词： angiostatin gene therapy pancreatic cancer vector angiogenesis liposome antiangiogenesis dormancy therapy

Muscarinic Receptor Agonists Protect Cultured Bovine Trabecular Meshwork Cells against Apoptosis Induced by Dexamethasone

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眼科学报 2004年第1期20卷 42-47页

作者： Yajuan Gu1,Shujun Zeng2,PengXin Qiu2,Yuping Wu2,Dawei Peng2,Guangmei Yan11department of Pharmacology, zhongshan medical college, sun yat-sen university, guangzhou 510089, china2zhongshan Ophthalmic Center, sun yat-sen university, guangzhou 510060, china department of Pharmacology Zhongshan Medical College Sun Yat-sen University Guangzhou 510089 China.

Purpose:To study whether muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by ***:The third to fifth passags of bovine trabecular meshwork cells were grown to confluence and incubated for 1～14 days in growth media with dexamethasone or pretreatment of pilocarpine or *** cultures were evaluated for apoptosis by phase-contrast microscopy, fluorescence microscopy, DNA laddering and flow cytometric ***:Dexamethasone (0.24～0.96 mmol·L-1) induced apoptosis of trabecular mes-hwork cells in a dose and time-dependent *** 0.48 mmol·L-1 dexametha-sone-treatment, 1.84 mmol·L-1 of pilocarpine or 2.74 mmol·L-1 of carbachol added could significantly reduce apoptotic ***:Muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone. Eye Science 2004;20:42-47.

关键词： apoptosis dexamethasone pilocarpine carbachol trabecular meshwork glaucoma

Reflection of plane electromagnetic waves from the surface of a perfect conductor moving in an arbitrary direction

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Chinese Science Bulletin 2000年第17期45卷 1564-1569页

作者： HE Qicai & HUANG Yaoxiong1. department of Physics, sun yat-sen university of medical Sciences, guangzhou 510089, china 2. Institute of Biomedical Engineering, Ji’nan university, guangzhou 510632, china Correspondence should be addressed to Huang Yaoxiong department of Physics Sun Yat-sen University of Medical Sciences Guangzhou China Institute of Biomedical Engineering Ji’nan University Guangzhou China

The reflection of plane electromagnetic waves (TE wave and TM wave) from a perfect conductor which moves in an arbitrary direction is investigated. Based on Maxwell’s equations and the boundary conditions for moving boundary, the relation between the field vectors of reflected and incident waves, and the reflection coefficient are derived. The energy balance between incident and reflected waves, the force exerted by electromagnetic waves to the moving conductor are also discussed and some new conclusions are suggested for notice.

关键词： plane electromagnetic wave moving conductor reflection coefficient radiation pressure.

Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 473-479页

作者： Jia-Hai LU Ding-Mei ZHANG Guo-Ling WANG Zhong-Min GUO Juan LI Bing-Yan TAN Li-Ping OU-YANG Wen-Hua LING Xin-Bing YU Nan-Shan ZHONG~1 The Public Health School of sun yat-sen university,guangzhou 510080,china 1 guangzhou Institute of Respiratory Diseases,guangzhou medical college,guangzhou 510120,china The Public Health School of sun yat-sen university Guangzhou 510080 China. jiahailu@***

The non-structural proteins (nsp or replicase proteins) of coronaviruses are relativelyconserved and can be effective targets for *** studies have been conducted into the function of thesevere acute respiratory syndrome coronavirus (SARS-CoV) *** this study,bioinformatics methodswere employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD *** and sequential comparison was performed to analyze the mutation trend of the polymerasensp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool(BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that thensp5 gene was steady during the epidemic process and the protein was homologous with other coronavirusnsp5 *** protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation forfurther exploration of the protein's biological function,and contributed to the search for anti-SARS-CoVdrugs.

关键词： severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) replicase secondary structure 3-D structure