Microtubule stability and MAP1B upregulation control neuritogenesis in CAD cells

作     者：Wen LI~(2,5)Jin-tang XIA~3 Yue FENG~(4,5)2 Laboratory of Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China 3 Department of General Surgery,The First People's Hospital of Guangzhou,Guangzhou 510180,China 4 Department of Pharmacology,Emory University School of Medicine,USA 

作者机构：Laboratory of Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou China Department of General Surgery The First People's Hospital of Guangzhou Guangzhou China Department of Pharmacology Emory University School of Medicine USA 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第9期

页      面：1119-1126页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：Project partly supported by NIH grant 5 PO1 HD35576(Project Ⅲ)to YF and to PG and a grant from Guangdong Science Foundation(№5001776) 

主　　题：microtubule stability microtubule associated protein 1B neuritogenesis 

摘      要：Aim:To study the role of microtubule dynamics and microtubule associated pro-tein 1B(MAP1B)in regulation of the neurite extension in CAD catecholaminergicneuronal cell ***:The neuritogenesis of the CAD cells was abolishedby inhibiting microtubule polymerization with nocodazole and by blocking micro-tubule depolymerization with ***1B and tubulin protein expression wasdetected by Western *** staining of tubulins was observedby fluorescent and confocal ***:Microtubule dynamics wasessential for CAD neurite *** analysis revealed that neurite exten-sion was much more sensitive to nocodazole than to taxol,suggesting a func-tional requirement for highly active microtubule assembly.A remarkableupregulation of MAP IB protein was detected during neurite extension accompa-nied with increased microtubule ***:Upregulation of MAP1Bleads to the stabilization of newly formed microtubules in the developing neurites,which in turn promotes neurite extension.