Thermal preconditioning protected cerebellar granule neurons of rats by modulating HSP70 expression
Thermal preconditioning protected cerebellar granule neurons of rats by modulating HSP70 expression作者机构：Department of Pharmacology Zhongshan Medical College Sun YatSen University Guangzhou 510089
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2004年第25卷第4期
页 面：60-63页
核心收录：
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：Project supported by the National Natural Science Foundation of Outstanding Young Scientist No 39625022
主 题：thermal preconditioning heat heat shock proteins cerebellum neurons apoptosis antisense oligodeoxynucleotides Western blotting
摘 要：AIM: To explore the possibility that expressions of different heat shock proteins (HSPs) are specifically involved in the protection of thermal preconditioning against apoptosis of cerebellar granule neurons (CGNs) induced by repolarization. METHODS: Western blotting was used to detect expressions of HSP27, HSP70, and HSP90 in- duced by thermal preconditioning (TP) in CGNs; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of HSP70 mRNA; apoptosis of CGNs was induced by switching culture medium containing KCl 25 mmol/L to one containing KCl 5 mmol/L. RESULTS: No expression of HSP27 in cerebellar granule neurons was observed with TP at 44 oC. Expression of HSP90 was obvious in CGNs both without and with TP at 44 oC for different periods. Expression of HSP70 protein in CGNs was lower with TP at 44 oC for 5 min, but it increased gradually after the period was prolonged to 30, 60, or 90 min. HSP70 mRNA was detected after TP 44 oC for 30, 60, and 90 min and increased gradually with time. HSP70 antisense oligodeoxynucleotides 10 μmol/Lfor 72 h inhibited the protective effects of TP at 44 oC on apoptosis of CGNs induced by repolarization. CONCLUSION: HSP70 is involved in protective effects of thermal preconditioning on apoptosis in cerebellar granule neurons induced by repolarization.
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