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Changes in Activities of Antioxidant-Related Enzymes in Leaves of Resistant and Susceptible Wheat Inoculated with Rhizoctonia cerealis

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Agricultural Sciences in China 2011年第4期10卷 526-533页

作者： LIU Hong-xia XIN zhi-yong ZHANG Zeng-yan National Key Facility for Crop Gene Resources and Genetic Improvement~Institute of Crop Sciences Chinese Academy of AgriculturalSciences Beijing 100081 P.R. China

In this study, the changes in activities of peroxidase （POD）, glutathione S-transferase （GST）, catalase （CAT）, and superoxide distmuase （SOD） in leaves of four wheat cultivars, including resistant cultivars Shannong 0431 and Shanhongmai, a mildsusceptible cultivar Lumai 21, and a susceptible cultivar Wenmai 6, were investigated to explore if these antioxidantrelated enzymes were involved in wheat defense response to Rhizoctonia cerealis attack. Results showed that the activities of these enzymes in different wheat cultivars varied greatly after challenging with R. cerealis. The POD activities in resistant cultivars Shannong 0431 and Shanhongmai increased much quicker after the inoculation and displayed much higher activity than that in susceptible cultivar Wenmai 6. In contrast, POD activity showed a slow accumulation and a delayed peak time. The activities of GST and CAT in Shannong 0431 and Lumai 21 increased obviously within 48 h post inoculation （hpi）, whereas SOD activity decreased dramatically within 24 hpi in Shannong 0431 and 24-48 hpi in Lumai 21. In susceptible cultivar Wenmai 6, the SOD and CAT activities didn’t change obviously, whereas the activity of GST was decreased. The activities of CAT and GST in Shanhongmai did not show significant difference compared with those in Wenmai 6. Results suggested that POD activity may be positively associated with wheat defense to R. cerealis.

关键词： wheat Rhizoctonia cerealis antioxidant-related enzymes peroxidase

Improving naive Bayes classifier by dividing its decision regions

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Journal of Zhejiang University-Science C(Computers and Electronics) 2011年第8期12卷 647-657页

作者： zhi-yong yan Gong-fu XU Yun-he PAN Institute of Artificial Intelligence Zhejiang University Hangzhou 310027 China

Classification can be regarded as dividing the data space into decision regions separated by decision *** this paper we analyze decision tree algorithms and the NBTree algorithm from this ***,a decision tree can be regarded as a classifier tree,in which each classifier on a non-root node is trained in decision regions of the classifier on the parent ***,the NBTree algorithm,which generates a classifier tree with the C4.5 algorithm and the naive Bayes classifier as the root and leaf classifiers respectively,can also be regarded as training naive Bayes classifiers in decision regions of the C4.5 *** propose a second division (SD) algorithm and three soft second division (SD-soft) algorithms to train classifiers in decision regions of the naive Bayes *** four novel algorithms all generate two-level classifier trees with the naive Bayes classifier as root *** SD and three SD-soft algorithms can make good use of both the information contained in instances near decision boundaries,and those that may be ignored by the naive Bayes ***,we conduct experiments on 30 data sets from the UC Irvine (UCI) *** results show that the SD algorithm can obtain better generali-zation abilities than the NBTree and the averaged one-dependence estimators (AODE) algorithms when using the C4.5 algorithm and support vector machine (SVM) as leaf *** experiments indicate that our three SD-soft algorithms can achieve better generalization abilities than the SD algorithm when argument values are selected appropriately.

关键词： Naive Bayes classifier Decision region NBTree C4.5 algorithm Support vector machine (SVM)

Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

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Biomedical and Environmental Sciences 2009年第4期22卷 354-358页

作者： HAI-TAO WANG BIN WANG zhi-JUN LIU zhi-QIANG BAI LING LI HAI-yan LIU DONG-MENG QIAN zhi-yong yan XU-XIA SONG Department of Microbiology Qingdao Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266071 Shandong China Qingdao Center for Disease Control and Prevention Qingdao 266033 Shandong China LRB 270E University of Massachusetts Medical School Worcester MA. 01605 USA

Objective To explore the change of endogenic nerve growth factor （NGF） expression in human glioma cells infected with human cytomegalovirus （HCMV）. Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD 169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group （P〈0.05）. Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.

关键词： Human cytomegalovirus U251 cells Nerve growth factor

Genetic Diversity Caused by Environmental Stress in Natural Populations of Niupidujuan as Revealed by RAPD Technique

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Chemical Research in Chinese Universities 2011年第4期27卷 641-645页

作者： DU Ying-da XING Ming yanG zhi-yong LIU yan-fei CHEN Xia College of Life Science Jilin University Changchun 130012 P. R. China

Multiplex environmental factors are generally expected to have significant effects on genetic diversity of plant *** this study,randomly amplified polymorphic DNA（RAPD） technique was used to reveal the genetic diversity in the same species of four populations collected from Niupidujuan（Rhododendron chrysanthum） at different altitudes,an endangered species,endemic to Northeast ***,twenty informative and reproducible primers were chosen for final RAPD analysis.A total of 152 clear bands were obtained,including 143 polymorphic *** the help of POPGENE software,the poly rate was calculated to be 94.07% and the evenness of amplified bands for every primer was ***,the mean observed number of alleles was 1.7265 with an effective number of *** examination of the gene indicated a diversity of 0.2162 with an information diversity index of *** these data,the clustering blurred analysis was performed with the aid of NTSYS-pc software to define the Nei＇s gene diversity and the Shannon information diversity index of the four plant *** relationships between the genetic diversity indexes on the one hand and the geographic and climatic factors on the other hand were estimated by the Pearson correlation with SPSS 11.0 *** results of the correlation analysis show that there were significant（P〈0.05） or highly significant（P〈0.01） correlations between each of the genetic diversity indexes and the different temperature which were mainly caused by the altitude different populations *** data highlight the importance of native populations in shaping the spatial genetic structure in Niupidujuan.

关键词： Environmental stress Genetic diversity Randomly amplified polymorphic DNA(RAPD) Clustering blurred analysis

Analysis of Norditerpenoid Alkaloids Extracted from Aconitum sinomantanum Nakai by Electrospray Ionization Tandem Mass Spectrometry

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Chemical Research in Chinese Universities 2006年第3期22卷 343-346页

作者： XU Qing-xuan YUE Hao LIU zhi-qiang WANG yong yan Cun-yu LIU Shu-ying Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China

Electrospray ionization mass spectrometry（ESI-MS） was applied simultaneously in determining norditerpenoid alkaloids from the roots of Aconitum sinomantanum Nakai （RAS） based on molecular mass information. The tandem mass spectra（ ESI-MS^n） provided the alkaloidal structural information, through which the existence of these alkaloids was further confirmed. Accordingly, six known norditerpenoid alkaloids were simultaneously determined on the basis of their ESI-MS^n spectra. Furthermore, based on the diagnostic fragmentation pathways of alkaloidal MS^n, a rapid method for direct detection and characterization of alkaloids from an ethanolic extract of RAS was described.

关键词： Electrospray ionization tandem mass spectrometry( ESI-MS^n) Norditerpenoid alkaloid Aconitum sinomantanum Nakai

Using the SELDI ProteinChip System to Detect Changes in Protein Expression in Vero Cells after Infection

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中国病毒学 2007年第1期22卷 68-73页

作者： zhi-jun LIU Bin WANG zhi-yong yan Xu-xia SONG Dong-meng QIAN zhi-qiang BAI Department of Microbiology Qingdao Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266021China

人的疱疹单一的病毒(HSV-1 ) 1 引起美容，眼睛，并且 encephalitic 疾病并且与潜伏的感染和癌症被联系。这里，我们开发了由使用 SELDI 蛋白质薄片在在 vitro 有教养的 Vero 房间检测蛋白质表示的变化在蛋白质水平学习 HSV-1 感染的致... 详细信息

人的疱疹单一的病毒(HSV-1 ) 1 引起美容，眼睛，并且 encephalitic 疾病并且与潜伏的感染和癌症被联系。这里，我们开发了由使用 SELDI 蛋白质薄片在在 vitro 有教养的 Vero 房间检测蛋白质表示的变化在蛋白质水平学习 HSV-1 感染的致病的一个工具。在有为 12， 24 或 48 h 的 HSV-1 和文化的感染以后，房间被收获并且 lysed。IMAC3 数组被用于 SELDI-TOF-MS 在感染前后检测 proteomic 差别。薄片检测了一系列差别表示蛋白质山峰。有趣地，在 16 912 Da 的山峰和 17 581 Da 与 ISG15 的分子的团精确相应，它可以在感染的过程期间参予抗病毒的活动。因此，我们获得了的结果能用作一个基础学习在病毒和它的主人之间的 HSV-1 和相互作用的致病。另外，他们能为 HSV-1 感染的治疗在新治疗学的目标的发现帮助。关键词 SELDI 蛋白质薄片 - Vero 房间 - HSV-1 - 蛋白质表达式 CLC 数字 R373 基础项目：中国(30540075 ) 的国家自然科学基础；

关键词： SELDI Protein Chip Vero Cells HSV-1 Protein Expression

HCMV Infection Depress NGF Expression in Human Glioma Cells

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Virologica Sinica 2009年第3期24卷 209-214页

作者： Hai-tao WANG Bin WANG zhi-jun LIU zhi-qiang BAI Ling LI Dong-meng QIAN zhi-yong yan Xu-xia SONG Department of Microbiology Qingdao Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266071 China Qingdao Center for Disease control and Prevention Qingdao 266033 China RB 270E University of Massachusetts Medical School Worcester MA. 01605 USA

Human cytomegalovirus （HCMV） is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia ceils by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

关键词： Human cytomegalovirus (HCMV) U251 cell Nerve growth factor (NGF)

Delayed ethyl pyruvate therapy attenuates experimental severe acute pancreatitis via reduced serum high mobility group box 1 levels in rats

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World Journal of Gastroenterology 2008年第28期14卷 4546-4550页

作者： zhi-yong yang yan Ling Tao Yin Jing Tao Jiong-Xin Xiong He-Shui Wu Chun-You Wang Department of Pancreatic Surgery Xiehe Hospital Tongji Medical College Huazhong University of Sciecne and Technology Wuhan 430022 Hubei Province China

AIM： To investigate the effect of delayed ethyl pyruvate （EP） delivery on distant organ injury, survival time and serum high mobility group box 1 （HMGB1） levels in rats with experimental severe acute pancreatitis （SAP）. METHODS： A SAP model was induced by retrograde injection of artificial bile into the pancreatic ducts of rats. Animals were divided randomly into three groups （n = 32 in each group）： sham group, SAP group and delayed EP treatment group. The rats in the delayed EP treatment group received EP （30 mg/kg） at 12 h, 18 h and 30 h after induction of SAP. Animals were sacrificed, and samples were obtained at 24 h and 48 h after induction of SAP. Serum HMGB1, aspartate arninotransferase （AST）, alanine arninotransferase （ALT）, blood urea nitrogen （BUN）, and creatinine （Cr） levels were measured. Lung wet-to-dry-weight （W/D） ratios and histological scores were calculated to evaluate lung injury. Additional experiments were performed between SAP and delayed EP treatment groups to study the influence of EP on survival times of SAP rats. RESULTS： Delayed EP treatment significantly reduced serum HMGB1 levels, and protected against liver, renal and lung injury with reduced lung W/D ratios （8.22 ±0.42 vs 9.76 ± 0.45, P 〈 0.01）, pulmonary histological scores （7.1 ± 0.7 vs 8.4 ± 1.1, P 〈 0.01）, serum AST （667 ± 103 vs 1 368 ± 271, P 〈 0.01）, ALT （446 ± 91 vs 653 ± 98, P 〈 0.01） and Cr （1.2 ± 0.3 vs 1.8 ± 0.3, P 〈 0.01） levels. SAP rats had a median survival time of 44 h. Delayed EP treatment significantly prolonged median survival time to 72 h （P 〈 0.01）. CONCLUSION： Delayed EP therapy protects against distant organ injury and prolongs survival time via reduced serum HMGBllevels in rats with experimental SAP. EP may potentially serve as an effective new therapeutic option against the inflammatory response and multiple organ dysfunction syndrome （MODS） in SAP patients.

关键词： Severe acute pancreatitis Ethyl pyruvate High mobility group box 1 multiple organ dysfunction syndrome Survival time

Human Cytomegalovirus Infection Inhibits the Differentiation of Human Hippocampus Neural Precursor Cells into Astrocytes

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Virologica Sinica 2009年第6期24卷 552-558页

作者： Ling LI Bin WANG Peng LI zhi-qiang BAI Hai-tao WANG Hai-yan LIU Dong-meng QIAN Xiu-xia SONG Shou-yi DING zhi-yong yan Guang-yu JIANG Department of Histology and Embryology Qingdao University Medical College Qingdao 266071 China Department of Microbiology Qingdao University Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266071 China

HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective of the present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural precursor cell (NPCs) differentiation in vitro. Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. The identification and purity of the NPCs were confirmed by using immunofluorescence to detect the expression of the NPCs marker-Nestin. To drive NPCs differentiation, bFGF and EGF were withdrawn from the medium and replaced with FBS (10%). HCMV AD169 (MOI=5) was added into the differentiation medium at the onset of the differentiation. After 7 days of differentiation, in order to confirm whether NPCs are permissive for HCMV infection, immunofluorescence was used to stain for the presence of immediate early (IE) and late (pp65) HCMV proteins in the infected cells. The effects of HCMV infection on NPCs’ differentiation was observed by detecting the ratio of nestin and GFAP positive cells with confocal microscopy and immunofluorescence. The data showed that 95%±8% of the cells (passage 4-8) cultured were Nestin positive which suggested that majority of the cells were NPCs. On day 7 postinfection, most of the infected cells were IE and PP65 positive. The percentage of Nestin-positive cells were 93%±10% and 50%±19% (t=6.03, p<0.01) and those of GFAP-positive cells were 55±17% and 81%±11% (t=3.77, p<0.01) in HCMV treated and control groups respectively. These findings indicate that NPCs are HCMV permissive cells and HCMV (AD 169) infection suppresses the differentiation of Hippocampus-genetic human NPCs into astrocytes. These effects may provide part of the explanation for the abnormalities in brain development associated with congenital HCMV infection.

关键词： Human cytomegalovirus (HCMV) Neural stem cells Cell differentiation Hippocampus Nestin