Genetic Diversity Caused by Environmental Stress in Natural Populations of Niupidujuan as Revealed by RAPD Technique

作     者：DU Ying-da XING Ming YANG Zhi-yong LIU Yan-fei CHEN Xia 

作者机构：College of Life Science Jilin University Changchun 130012 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2011年第27卷第4期

页      面：641-645页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 090102[农学-作物遗传育种] 

基　　金：Supported by the Project of Development and Reform Commission of Jilin Province China 

主　　题：Environmental stress Genetic diversity Randomly amplified polymorphic DNA(RAPD) Clustering blurred analysis 

摘      要：Multiplex environmental factors are generally expected to have significant effects on genetic diversity of plant *** this study,randomly amplified polymorphic DNA（RAPD） technique was used to reveal the genetic diversity in the same species of four populations collected from Niupidujuan（Rhododendron chrysanthum） at different altitudes,an endangered species,endemic to Northeast ***,twenty informative and reproducible primers were chosen for final RAPD analysis.A total of 152 clear bands were obtained,including 143 polymorphic *** the help of POPGENE software,the poly rate was calculated to be 94.07% and the evenness of amplified bands for every primer was ***,the mean observed number of alleles was 1.7265 with an effective number of *** examination of the gene indicated a diversity of 0.2162 with an information diversity index of *** these data,the clustering blurred analysis was performed with the aid of NTSYS-pc software to define the Nei＇s gene diversity and the Shannon information diversity index of the four plant *** relationships between the genetic diversity indexes on the one hand and the geographic and climatic factors on the other hand were estimated by the Pearson correlation with SPSS 11.0 *** results of the correlation analysis show that there were significant（P〈0.05） or highly significant（P〈0.01） correlations between each of the genetic diversity indexes and the different temperature which were mainly caused by the altitude different populations *** data highlight the importance of native populations in shaping the spatial genetic structure in Niupidujuan.