Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

作     者：HAI-TAO WANG BIN WANG ZHI-JUN LIU ZHI-QIANG BAI LING LI HAI-YAN LIU DONG-MENG QIAN ZHI-YONG YAN XU-XIA SONG 

作者机构：Department of Microbiology Qingdao Medical College and Key Laboratory of Medicine and Biotechnology of Qingdao Qingdao University Medical College Qingdao 266071 Shandong China Qingdao Center for Disease Control and Prevention Qingdao 266033 Shandong China LRB 270E University of Massachusetts Medical School Worcester MA. 01605 USA 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2009年第22卷第4期

页      面：354-358页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China, (No. 30770105) Mt. Tai Scholar Construction Engineering Special Foundation of Shandong Province 

主　　题：Human cytomegalovirus U251 cells Nerve growth factor 

摘      要：Objective To explore the change of endogenic nerve growth factor （NGF） expression in human glioma cells infected with human cytomegalovirus （HCMV）. Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD 169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group （P〈0.05）. Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.