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Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method

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Journal of Environmental Sciences 2007年第1期19卷 60-66页

作者： LIU Xin-chun ZHANG yu YANG Min WANG zhen-yu LV Wen-zhou State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China

The bacterial community structures in two sewage treatment plants with different processes and performance were investigated by denaturing gradient gel electrophoresis （DGGE） of nested polymerase chain reaction （nested PCR） amplified 16S rRNA gene fragments with group-specific primers. Samples of raw sewage and treated effluents were amplified using the whole-cell PCR method, and the activated sludge samples were amplified using the extracted genomic DNA before the PCR products were loaded on the same DGGE gel for bacterial community analysis. Ammonia-oxidizing bacterial and actinomycetic community analysis were also carried out to investigate the relationship between specific population structures and system or sludge performance. The two plants demonstrated a similarity in bacterial community structures of raw sewage and activated sludge, but they had different effluent populations. Many dominant bacterial populations of raw sewage did not appear in the activated sludge samples, suggesting that the dominant bacterial populations in raw sewage might not play an important role during wastewater treatment. Although the two plants had different sludge properties in terms of settleability and foam forming ability, they demonstrated similar actinomycetic community structures. For activated sludge with bad settling performance, the treated water presented a similar DGGE pattern with that of activated sludge, indicating the nonselective washout of bacteria from the system. The plant with better ammonium removal efficiency showed higher ammonia-oxidizing bacteria species richness. Analysis of sequencing results showed that the major populations in raw sewage were uncultured bacterium, while in activated sludge the predominant populations were beta proteobacteria.

关键词： activated sludge wastewater treatment sewage community analysis PCR-DGGE

Research on the mechanism of the two-dimensional ultrasonic surface burnishing process to enhance the wear resistance for aluminum alloy

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Friction 2024年第3期12卷 490-509页

作者： zhen-yu zhou Qiu-Yang zhenG yu LI Cong DING Guang-Jian PENG Zhong-yu PIAO College of Mechanical Engineering Zhejiang University of TechnologyHangzhou 310023China Key Laboratory of Special Purpose Equipment and Advanced Processing Technology Ministry of Education and Zhejiang ProvinceZhejiang University of TechnologyHangzhou 310014China

The gradient nanostructure is machined on the aluminum(Al)alloy by the two-dimensional ultrasonic surface burnishing process(2D-USBP).The mechanism of why the gradient nanostructure enhances wear resistance is *** mechanical properties and microstructure characterization for the gradient nanostructure are performed by operating a nanoindenter,transmission electron microscopy(TEM),and electron backscattered diffraction(EBSD).Dry wear tests are performed on the samples before and after machining to evaluate the wear resistance and *** effect of the gradient nanostructure on the wear resistance is explored by developing the crystal plasticity(CP)finite element and molecular dynamics(MD)*** characterization results show that the 2D-USBP sample prepared a gradient structure of~600μm thick on the aluminum surface,increasing the surface hardness from 1.13 to 1.71 GPa and reducing the elastic modulus from 78.84 to 70.14 *** optimization of the surface microstructure and the increase of the mechanical properties effectively enhance the wear resistance of the sample,with 41.20%,39.07%,and 54.58% of the wear scar areas for the 2D-USBP treated samples to the original samples under 5,10,and 15 N loads,*** gradient nanostructure hinders the slip of dislocations inside the sample during the wear process and reduces the size and scope of plastic deformation;meanwhile,the resistance to deformation,adhesion,and crack initiation and propagation of the sample surface is improved,resulting in enhanced wear resistance.

关键词： structural superlubricity lattice registry elastic deformation strained solitons crystal plasticity molecular dynamics simulations

Therapeutic effects of herbal formula Huangqisan on metabolic disorders via SREBF1,SCD1 and AMPK signaling pathway

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Journal of Integrative Medicine 2021年第2期19卷 167-176页

作者： Ya-lei Liu zhen-yu zhou Min Gao Guang Ji Cheng Huang Sheng-jie Fan School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghai 201203China College of Pharmacy Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Institute of Digestive Diseases Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China

Objective:Metabolic syndrome is a complex medical condition that has become an alarming epidemic,but an effective therapy for this disease is still *** use of the herbal formula Huangqisan(HQS)to treat diabetes is documented in the Chinese medical literature as early as 1117 A.D.;however,its therapeutic effects and underlying mechanisms remain ***:To investigate the beneficial effects of HQS on metabolic disorders,high-fat diet-induced obesity(DIO),leptin receptor dysfunction(db/db)and low-density lipoprotein receptor-knockout(LDLR^(-/-))mice were *** mice were treated with either HQS or ***,liver tissue,white fat tissue and brown adipose tissue were harvested at the end of the *** disease-related parameters were evaluated to test effects of HQS against diabetes,obesity and *** arches from LDLR^(-/-)mice were analyzed to investigate the effects of HQS on ***-sequence,quantitative real-time polymerase chain reaction and Western blot were performed to investigate the mechanisms of HQS against metabolic ***:HQS lowered body weight,fasting blood glucose and serum lipid levels and improved glucose tolerance and insulin sensitivity in DIO mice and db/db mice(P<0.05).HQS also blocked atherosclerotic plaque formation in LDLR^(^(-/-)) *** suppressed de novo lipid synthesis by reducing the expression of messenger RNA for sterol regulatory element-binding factor 1,stearyl coenzyme A desaturase 1 and fatty acid synthase,and enhancing adenosine 5’-monophosphate-activated protein kinase signaling in both in vivo and in vitro experiments,indicating potential mechanisms for HQS’s activity against ***:HQS is effective for reversing metabolic disorder and has the potential to be used as therapy for metabolic syndrome.

关键词： Tradition Chinese medicine Diabetes Hyperlipidemia Obesity Atherosclerosis

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

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Journal of Pharmaceutical Analysis 2018年第4期8卷 240-249页

作者： Xing-Xin Yang yu-zhen zhou Feng Xu Jie yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai Department of Natural Medicines School of Pharmaceutical Sciences Peking University Department of Chemical Biology School of Pharmaceutical Sciences Peking University College of Pharmaceutical Science Yunnan University of Traditional Chinese Medicine

Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry（LC/MS） method,also known as screening method for mitochondria-targeted bioactive constituents（SM-MBC）. This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines（TCMs）, including Puerariae Radix（PR） and Chuanxiong Radix（CR）. A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on Hep G2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts.

关键词： Mitochondria Bioactive constituents Traditional Chinese medicines Centrifugal ultrafiltration Liquid chromatography/mass spectrometry

Multiple advanced surgical techniques to treat acquired seminal duct obstruction

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Asian Journal of Andrology 2014年第6期16卷 912-916页

作者： Hong-Tao Jiang Qian yuan yu Liu Zeng-Qin Liu zhen-yu zhou Ke-Feng Xiao Jiang-Gen Yang Department of Urology Shenzhen People's Hospital Shenzhen 518020 China Department of Laboratory Medicine Shenzhen People's Hospital Shenzhen 518020 China

The aim of this study was to evaluate the outcomes of multiple advanced surgical treatments （i.e. microsurgery, laparoscopic surgery and endoscopic surgery）for acquired obstructive azoospermia. We analyzed the surgical outcomes of 51 patients with suspected acquired obstructive azoospermia consecutively who enrolled at our center between January 2009 and May 2013. Modified vasoepididymostomy, laparoscopically assisted vasovasostomy and transurethral incision of the ejaculatory duct with holmium laser were chosen and performed based on the different obstruction sites. The mean postoperative follow-up time was 22 months （range： 9 months to 52 months）. Semen analyses were initiated at four postoperative weeks, followed by trimonthly （months 3, 6, 9 and 12） semen analyses, until no sperm was found at 12 months or until pregnancy was achieved. Patency was defined as 〉10,000 sperm m1-1 of semen. The obstruction sites, postoperative patency and natural pregnancy rate were recorded. Of 51 patients, 47 underwent bilateral or unilateral surgical reconstruction; the other four patients were unable to be treated with surgical reconstruction because of pelvic vas or intratesticular tubules obstruction. The reconstruction rate was 92.2% （47/51）, and the patency rate and natural pregnancy rate were 89.4% （42/47） and 38.1% （16/42）, respectively. No severe complications were observed. Using multiple advanced surgical techniques, more extensive range of seminal duct obstruction was accessible and correctable; thus, a favorable patency and pregnancy rate can be achieved.

关键词： azoospermia endoscopy laparoscopy male infertility microsurgery

Effect of Nitrogen Fertilizers on Movement and Transformation of Phosphorus in an Acid Soil

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Pedosphere 2005年第4期15卷 424-431页

作者： DU zhen-yu zhou Jian-Min WANG Huo-Yan DU Chang-Wen CHEN Xiao-Qin State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing210008 （China）.

The effects of two different nitrogen fertilizers （urea and NH4C1） with monocalcium phosphate （MCP） on the movement and transformation of fertilizer P in soil microsites along with soil pH changes at different distances from the fertilizer application site were studied in an incubation experiment. A highly acidic red soil （Ultisol, pH 4.57） from south China with MCP fertilizer alone or in combination with NH4C1 or urea was added to the surface of soil cylinders and packed in wax blocks. After 7 and 28 days, the extraction and analysis of each 2 mm layer from the interface of the soil and fertilizer showed that added NH4C1 or urea did not change the movement distance of fertilizer P. However, P transformation was significantly affected （P 〈 0.05）. After 7 days, at 0-8 mm distance from the fertilizer site the addition of urea significantly decreased the water-extractable P concentration; however, after 28 days the effect of N addition had disappeared. Also,at limited distances close to the fertilizer site NH4Cl application with MCP significantly increased acid-extractable P and available P, while with the addition of urea they significantly decreased. Compared with application of MCP alone,addition of urea significantly increased soil pH in fertilizer microsites, whereas the addition of NH4Cl significantly decreased soil pH.

关键词： NH4Cl P movement P transformation red soil urea

New Developments in Treatments of Tibial Plateau Fractures

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Chinese Medical Journal 2017年第21期130卷 2635-2638页

作者： Ya-Ke Liu zhen-yu zhou Fan Liu Department of Orthopedics Affiliated Hospital of Nantong University Nantong Jiangsu 226001 China

Tibial plateau fractures account for 1.66–2.0% of all fractures in adults and about 8% of fractures in the elderly.[1] Tibial plateau fractures should be considered as complex injuries representing a wide fracture spectrum： soft-tissue compromise, neurovascular damage, compartment syndrome, and ligament and meniscus tears, which remain a big challenge, even for experienced orthopedic trauma surgeons. Although the best treatment modality remains controversial, we aimed to present new developments in the treatment of tibial plateau fractures for reference.

关键词： Diagnose Tibial Plateau Fractures Treatment

The importance of laparoscopy in the surgical reconstruction of inguinal vas injury

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Asian Journal of Andrology 2017年第2期19卷 258-259页

作者： Hong-Tao Jiang Ji-Nan Guo Zeng-Qin Liu Qian yuan zhen-yu zhou yu Liu Ke-Feng Xiao Jiang-Gen Yang Department of Urology Minimally Invasive Urology of Shenzhen Research and Development Center of Medical Engineering and Technology Shenzhen People's Hospital Jinan University Second Clinic Medical College Shenzhen 518020 China Department of Laboratory Medicine Shenzhen People's Hospital Shenzhen 518020. China

Dear Editor, We are very interested in the recent paper published by Chen et al.1 because we also have treated patients with iatrogenic vasal injury. We agree with the opinion in this paper that surgical reconstructi... 详细信息

关键词： The importance surgical reconstruction inguinal vas injury

Integration of Electrical Properties and Polarization Loss Modulation on Atomic Fe–N‑RGO for Boosting Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第2期17卷 517-532页

作者： Kaili Zhang yuefeng Yan zhen Wang Guansheng Ma Dechang Jia Xiaoxiao Huang yu zhou State Key Laboratory of Precision Welding&Joining of Materials and Structures Harbin Institute of TechnologyHarbin 150001People’s Republic of China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001People’s Republic of China MIIT Key Laboratory of Advanced Structural‑Functional Integration Materials&Green Manufacturing Technology Harbin Institute of TechnologyHarbin 150001People’s Republic of China

Developing effective strategies to regulate graphene’s conduction loss and polarization has become a key to expanding its application in the electromagnetic wave absorption(EMWA)*** on the unique energy band structure of graphene,regulating its bandgap and electrical properties by introducing heteroatoms is considered a feasible ***,metal-nitrogen doping reduced graphene oxide(M–N-RGO)was prepared by embedding a series of single metal atoms M–N_(4) sites(M=Mn,Fe,Co,Ni,Cu,Zn,Nb,Cd,and Sn)in RGO using an N-coordination atom-assisted *** composites had adjustable conductivity and polarization to optimize dielectric loss and impedance matching for efficient EMWA *** results showed that the minimum reflection loss(RL_(min))of Fe–N-RGO reaches−74.05 dB(2.0 mm)and the maximum effective absorption bandwidth(EAB_(max))is 7.05 GHz(1.89 mm)even with a low filler loading of only 1 wt%.Combined with X-ray absorption spectra(XAFS),atomic force microscopy,and density functional theory calculation analysis,the Fe–N_(4) can be used as the polarization center to increase dipole polarization,interface polarization and defect-induced polarization due to d-p orbital hybridization and structural ***,electron migration within the Fe further leads to conduction loss,thereby synergistically promoting energy *** study demonstrates the effectiveness of metal-nitrogen doping in regulating the graphene′s dielectric properties,which provides an important basis for further investigation of the loss mechanism.

关键词： Electromagnetic wave absorption Fe-N-RGO Dipole polarization Conduction loss Impedance matching