检索结果-南通市图书馆

基于KASP平台的转基因纯合体高通量检测方法的建立

在线全文

学校读者我要写书评

暂无评论

玉米科学 2024年第8期32卷 31-37页

作者：孙铭勖葛建镕朱维佳王蕊赵怡锟金肇阳赵紫薇朱少喜赵久然王凤格吉林农业大学农学院长春130118 北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室北京100097 扬州大学农学院江苏扬州225009 黑龙江大学现代农业与生态环境学院哈尔滨150008

以转基因玉米DBN9501为材料,设计一套包含1条位于基因组的共用引物、两条分别位于外源插入序列与外源插入序列等位基因组序列的竞争性引物,能分别扩增出外源片段与基因组片段的纯合体检测引物,建立一种基于KASP平台的高通量转基因玉米... 详细信息

以转基因玉米DBN9501为材料,设计一套包含1条位于基因组的共用引物、两条分别位于外源插入序列与外源插入序列等位基因组序列的竞争性引物,能分别扩增出外源片段与基因组片段的纯合体检测引物,建立一种基于KASP平台的高通量转基因玉米纯合体检测方法。利用DBN9501BC1和15份其他转基因玉米样品进行引物评估、体系优化以及特异性验证。结果显示,所设计的引物既能特异性地对DBN9501进行成分检测,又能对DBN9501BC1代中的纯合阳性、杂合阳性以及阴性3种基因型进行准确鉴别。

关键词：转基因玉米纯合体检测高通量 KASP平台分子标记

糯玉米子粒硬度与淀粉合成相关酶动态变化及相互关系

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

玉米科学 2024年第6期32卷 38-45页

作者：史亚兴董会刘辉徐丽樊艳丽俞嫒年史亚民卢柏山赵久然北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室北京100097

以鲜食糯玉米品种京科糯2000和苏玉糯2号为试验材料,比较授粉后不同时期淀粉合成相关酶活性、淀粉含量、含水量和子粒硬度的差异及各指标间的相互关系,利用Logistic方程比较两个品种子粒淀粉积累速率。结果表明,糯玉米子粒淀粉合成相关... 详细信息

以鲜食糯玉米品种京科糯2000和苏玉糯2号为试验材料,比较授粉后不同时期淀粉合成相关酶活性、淀粉含量、含水量和子粒硬度的差异及各指标间的相互关系,利用Logistic方程比较两个品种子粒淀粉积累速率。结果表明,糯玉米子粒淀粉合成相关酶AGPase活性和SSS活性对淀粉的合成具有重要的促进作用,且在品种间有差异。利用Logistic方程比较两个品种糯玉米子粒淀粉积累过程,分析表明,与苏玉糯2号相比,京科糯2000达到最大淀粉积累速率的天数(T_(max))延长1.62 d,淀粉活跃积累天数增加8.85 d,子粒淀粉积累速率最大时的生长量(W_(max))提高3.97%,最大淀粉积累速率(G_(max))降低12.60%。糯玉米子粒淀粉含量与子粒含水量呈极显著负相关,与子粒硬度呈极显著正相关。

关键词：糯玉米淀粉积累子粒含水量硬度

Dynamic changes of root proteome reveal diverse responsive proteins in maize subjected to cadmium stress

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第10期18卷 2193-2204页

作者： REN Wen LIU Ya ZHOU Miao-yi SHI Zi WANG Tian-yu zhao jiu-ran LI Yu Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Maize Research Center Beijing Academy of Agriculture & Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular BreedingBeijing 100097P.R.China

Toxic symptoms and tolerance mechanisms of heavy metal in maize are well documented. However, limited information is available regarding the changes in the proteome of maize seedling roots in response to cadmium(Cd) stress. Here, we employed an i TRAQ-based quantitative proteomic approach to characterize the dynamic alterations in the root proteome during early developmental in maize seedling. We conducted our proteomic experiments in three-day seedling subjected to Cd stress, using roots in four time points. We identified a total of 733, 307, 499, and 576 differentially abundant proteins after 12, 24, 48, or 72 h of treatment, respectively. These proteins displayed different functions, such as ribosomal synthesis, reactive oxygen species homeostasis, cell wall organization, cellular metabolism, and carbohydrate and energy metabolism. Of the 166 and 177 proteins with higher and lower abundance identified in at least two time points, 14 were common for three time points. We selected nine proteins to verify their expression using quantitative real-time PCR. Proteins involved in the ribosome pathway were especially responsive to Cd stress. Functional characterization of the proteins and the pathways identified in this study could help our understanding of the complicated molecular mechanism involved in Cd stress responses and create a list of candidate gene responsible for Cd tolerance in maize seeding roots.

关键词： cadmium stress iTRAQ proteomics maize seedling roots

在线全文

学校读者我要写书评

暂无评论

豫中南青贮玉米品种筛选及营养价值综合评价

玉米科学 2024年第8期32卷 8-16页

作者：袁刘正朱世蝶徐田军王会强王会涛袁曼曼吴浩柳家友赵久然漯河市农业科学院/河南省玉米防灾减灾工程技术研究中心河南漯河462000 北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室北京100097

为筛选出适合豫中南夏玉米区高产优质多抗的青贮玉米品种,以20个玉米品种为试验材料,对其生育期、干草产量等13个指标进行分析。结果表明,京科青贮516、京科968等6个品种的干草产量较高,抗逆性强。对干草产量、农艺性状及营养指标进行... 详细信息

为筛选出适合豫中南夏玉米区高产优质多抗的青贮玉米品种,以20个玉米品种为试验材料,对其生育期、干草产量等13个指标进行分析。结果表明,京科青贮516、京科968等6个品种的干草产量较高,抗逆性强。对干草产量、农艺性状及营养指标进行聚类分析,将20个玉米品种分为3个类群,其中,第1类群为京科青贮938等8个品种,其干草平均产量最高,抗逆性强,中性洗涤纤维和酸性洗涤纤维平均最高,饲料相对饲喂价值最低;第2类群为京科522,其农艺性状居于中等;第3类群为MC703等11个品种,其干草平均产量较高,平均粗蛋白含量等营养价值优良。从干草产量等综合分析,MC703、郑单919、京科青贮576、京科青贮516、京科968等5个品种表现较好,适宜作为青贮玉米在豫中南进行推广种植。

关键词：青贮玉米豫中南粮饲通用产量品质

Maize grain yield and water use efficiency in relation to climatic factors and plant population in northern China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2021年第12期20卷 3156-3169页

作者： LIU Yue-e HOU Peng HUANG Gui-rong ZHONG Xiu-li LI Hao-ru zhao jiu-ran LI Shao-kun MEI Xu-rong Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding/Maize Research Center Beijing Academy of Agriculture&Forestry SciencesBeijing 100097P.R.China Key Laboratory of Crop Physiology and Ecology Ministry of Agriculture and Rural Affairs/lnstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences/Key Laboratory of Dryland Farming AgricultureMinistry of Agriculture and Rural AffairsBeijing 100081P.R.China

Water scarcity has become a limiting factor for increasing crop *** ways to improve water use efficiency(WUE)has become an urgent task for Chinese *** understand the response of different maize populations to changes in precipitation and the effects of changes in maize populations on WUE,this study conducted maize population experiments using maize hybrids with different plant types(compact and semi compact)and different planting densities at 25 locations across *** was found that,as precipitation increased across different locations,maize grain yield first increased and then decreased,while WUE decreased *** the relationship between WUE and the main climatic factors,this study found that WUE was significantly and negatively correlated with precipitation(R(daily mean precipitation)and R(accumulated precipitation))and was positively correlated with temperature(TM(daily mean maximum temperature),T_(M-m)(T_(m),daily mean minimum temperature)and GDD(growing degree days))and solar radiation(Ra(daily mean solar radiation)and Ra(accumulated solar radiation))over different growth *** differences in maize grain yield,WUE and precipitation were found at different planting *** population densities were ranked as follows according to maize grain yield and WUE based on the multi-site experiment data:60000 plants ha^(-1)(P_(2))>90000 plants ha^(-1)(P_(3))>30000 plants ha^(-1)(P_(1)).Further analysis showed that,as maize population increased,water consumption increased significantly while soil evaporation decreased *** differences were found between the WUE of ZD958(compact type)and that of LD981(semi-compact type),as well as among the WUE values at different planting *** addition,choosing the optimum hybrid and planting density increased WUE by 21.70 and 14.92%,respectively,which showed that the hybrid played a more significant role than the planting density in improving WU

关键词： maize climatic factor water utilization characteristics water use efficiency hybrids planting density

用于玉米品种真实性鉴定的最优核心SNP位点集的研发

在线全文

学校读者我要写书评

暂无评论

作物学报 2024年第5期50卷 1115-1123页

作者：田红丽杨扬范亚明易红梅王蕊金石桥晋芳张云龙刘亚维王凤格赵久然北京市农林科学院玉米研究所/农业农村部农作物DNA指纹创新利用重点实验室(部省共建)/玉米DNA指纹及分子育种北京市重点实验室北京100097 全国农业技术推广服务中心北京100125

品种真实性是种子质量监测的一个重要指标。为建立准确可靠、快速简便、高通量、低成本的玉米品种真实性鉴定技术,本文利用200个核心SNP位点构建的5816个玉米杂交品种,3274个自交系的指纹数据,基于遗传算法、品种识别率评估确定了一套... 详细信息

品种真实性是种子质量监测的一个重要指标。为建立准确可靠、快速简便、高通量、低成本的玉米品种真实性鉴定技术,本文利用200个核心SNP位点构建的5816个玉米杂交品种,3274个自交系的指纹数据,基于遗传算法、品种识别率评估确定了一套高鉴别力的核心SNP位点集,包含96个SNP位点。这96个SNPs全部位于基因内区域,相对均匀分布在10对染色体上。采用上述杂交品种和自交系的指纹数据评估显示这96个位点具有较高多态性和品种区分能力,PIC、MAF、DP平均值分别为0.36、0.40、0.60和0.36、0.39、0.48,对杂交品种、自交系的品种识别率达到99.14%和99.24%。两两样品成对比较结果显示,99.99%的品种间差异位点数目≥3个,杂交品种和自交系中96.74%和95.67%的成对比较差异位点数目集中在30~65个和30~60个。基于221个主推杂交品种的40个SSR位点、96个SNP位点的基因型数据分析结果显示,这2组标记集的鉴定结果具有较高的一致性。综上所述,本研究报道了一套具有位点数量最少、区分能力最强,兼容多平台、适于自动化分型等优点的最优核心SNP集。期望位点集将在玉米品种真实性监测、种子质量控制中得到广泛应用,进而维护玉米种子市场秩序、保障育种者权利以及保护农民利益。

关键词：玉米品种真实性鉴定 SNP位点集高鉴别力

Exploring differentially expressed genes associated with fertility instability of S-type cytoplasmic male-sterility in maize by RNA-seq

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第8期16卷 1689-1699页

作者： SU Ai-guo SONG Wei SHI Zi zhao Yan-xin XING Jin-feng ZHANG Ru-yang LI Chun-hui LUO Mei-jie WANG Ji-dong zhao jiu-ran Maize Research Center Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular BreedingBeijing 100097P.R.China

The germplasm resources for the S-type male sterility is rich in maize and it is resistant to Bipolaris maydis race T and CI, but the commercial application of S-type cytoplasmic male sterility （CMS-S） in maize hybrid industry is greatly compromised because of its common fertility instability. Currently, the existence of multiple minor effect loci in specific nuclear genetic backgrounds was considered as the molecular mechanism for this phenomenon. In the present study, we evaluated the fertility segregation of the different populations with the fertility instable material FIL-H in two environments of Beijing and Hainan, China. Our results indicated that the fertility instability of FIL-H was regulated by multiple genes, and the expression of these genes was sensitive to environmental factors. Using RNA sequencing （RNA-seq） technology, transcriptomes of the sterile plants and partially fertile plants resulted from the backcross of FIL-HxJing 724 in Hainan were analyzed and 2 108 genes with different expression were identified, including 1 951 up-regulated and 157 down-regulated genes. The cluster analysis indicated that these differentially expressed genes （DEGs） might play roles in many biological processes, such as the energy production and conversion, carbohydrate metabolism and signal transduction. In addition, the path- way of the starch and sucrose metabolism was emphatically investigated to reveal the DEGs during the process of starch biosynthesis between sterile and partially fertile plants, which were related to the key catalytic enzymes, such as ADP-G pyrophosphorylase, starch synthase and starch branching enzyme. The up-regulation of these genes in the partially fertile plant may promote the starch accumulation in its pollen. Our data provide the important theoretical basis for the further exploration of the molecular mechanism for the fertility instability in CMS-S maize.

关键词： CMS-S fertility instability RNA-seq DEGs

基于不同玉米生态区主推品种探讨宜粒收关键种质构建方向

在线全文

学校读者我要写书评

暂无评论

玉米科学 2024年第3期32卷 20-25页

作者：付修义张华生赵久然陈传永陈琛吴珊珊张雪原张春原王元东北京市农林科学院玉米研究所北京100097

选育宜机械粒收玉米新品种是节约劳动成本、提高经济效益的重要途径。根据生产中不同生态区对宜机械粒收玉米品种改良需求,分析影响玉米机械粒收的主要特性指标,确立宜粒收玉米育种目标。本文基于不同生态区光热条件、主推品种、广泛应... 详细信息

选育宜机械粒收玉米新品种是节约劳动成本、提高经济效益的重要途径。根据生产中不同生态区对宜机械粒收玉米品种改良需求,分析影响玉米机械粒收的主要特性指标,确立宜粒收玉米育种目标。本文基于不同生态区光热条件、主推品种、广泛应用种质及杂种优势模式,探讨适宜不同玉米生态区的宜机械粒收关键种质的构建方向以及杂优模式利用与品种选育策略。同时对宜机械粒收种质特性改良与机收品种选育进行实践,为我国宜机械粒收玉米种源创新与新品种选育提供参考。

关键词：玉米宜机械粒收种质新品种选育

Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2007年第6期6卷 647-651页

作者： LI Hui-yong HUANG Su-hua SHI Yun-su SONG Yan-chun zhao jiu-ran WANG Feng-ge WANG Tian-yu LI Yu Institute of Food Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Maize Research Institute Beijing Academy of Agricultural and Forestry Sciences Beijing 100081 P.R.China

In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.

关键词： maize drought stress suppression subtractive hybridization (SSH) uniESTs