Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization
Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization作者机构：Institute of Food Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Maize Research Institute Beijing Academy of Agricultural and Forestry Sciences Beijing 100081 P.R.China
出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)
年 卷 期：2007年第6卷第6期
页 面：647-651页
基 金：Beijing Agricultural Innovative Platform-Beijing Natural Science Fund Program Project for International Scientific and Technological Cooperation-Discovery and Utilization of Drought-Resistant Genes and Allele Gene in Maize Germplasm Resources National Natural Science Foundation of China, NSFC, (305711 33) Ministry of Science and Technology of the People's Republic of China, MOST, (0502)
主 题：maize drought stress suppression subtractive hybridization (SSH) uniESTs
摘 要：In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.
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