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Identification of Circular RNA in sheep mammary gland using RNA-Seq technology

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Identification of Circular RNA in sheep mammary gland using ...

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Zhiyun Hao Jiqing Wang yuzhu Luo Shaobin Li Mengli Zhao Xiu Liu Jiang hu Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and TechnologyGansu Agricultural University

Lactation is primarily responsible for the survival rate of multiple-born lambs. Exploring the molecular mechanism underlying the mammary gland and lactation plays an important role on regulating milk yield and quality. Circular RNA（circRNA） is a particular type of non-coding RNA and has a large number of microRNA（miRNA） binding sites and act as miRNA sponges in cells. These circRNAs can remove the inhibition of miRNAs on their target genes and increasing expression of target genes. In several species, there was a little bit study on circRNA of mammary gland. However, no studies on circRNA of mammary gland in sheep have been reported so far. In our study, expression profile of circRNAs were investigated in mammary gland of two sheep breeds with different milk production characteristics, Gansu Alpine Merino（GAM, n=9） and Small Tailed Han sheep（STH, n=9） in peaking-lactating period using RNASeq. The 4,907 circRNAs were found and 33 were differentially expressed circRNAs between the two breed sheep. The 18 circRNAs were up-regulated and 15 circRNAs were down-regulated in STH. The potential target sites of circRNAs were predicted and the RNA-Seq results were validated by qRT-PCR. GO and KEGG results showed differentially expressed circRNAs were mainly enriched in cell proliferation, cell differentiation, signal transduction and other biological processes. This study provides an overview of the circRNA expression profile in the ovine mammary gland and the interaction between some key circRNAs and their target genes, aiming at improving our knowledge of the critical roles of circRNA in sheep milk synthesis.

关键词： CircRNA mammary gland sheep RNA-Seq differentially expressed circRNA

Decidual macrophage M1 polarization contributes to adverse pregnancy induced by Toxoplasma gondii infection

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Decidual macrophage M1 polarization contributes to adverse p...

第十二届全国免疫学学术大会

作者： Liu Xianbing Jiang Mengqi Ren Liqin Zhang Aihong Zhao Mingdong Zhang Haixia Jiang yuzhu hu Xuemei Binzhou Medical University

Recent evidence indicates that macrophages at the maternal-fetal interface adapt to a phenotype characterized by alternative activation(M2 polarization) and exhibit immunosuppressive functions that favor the maintenance of *** bias of M2 decidual macrophages toward M1 has been clinically linked to pregnancy-related complications,such as preeclampsia,and preterm *** aim of this study was to investigate the effect of Toxoplasma gondii infection on the bias of decidual macrophage polarization and its contribution to adverse pregnancy outcomes.A mouse model with adverse pregnancy outcome was established by infection with *** PRU *** expression levels of CD80,CD86,CD206,MHC-Ⅱ,iNOS,arginase-1,IL-10,TNF-α,and IL-12 in decidual macrophages of infection and control mice were *** results showed that the expression of CD206,MHC-Ⅱ,and arginase-1 considered as M2 markers was decreased in decidual macrophages after *** infection,whereas the expression of CD80,CD86,iNOS,and cytokines TNF-α and IL-12 considered as M1 markers was *** results indicated that *** infection was responsible for the bias of M2 decidual macrophages toward M1,which changes the immunosuppressive microenvironment at the maternal-fetal interface and contributes to adverse pregnancy outcomes.

关键词： Toxoplasma gondii decidual macrophages polarization adverse pregnancy outcomes

A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第4期17卷 470-477页

作者： yuzhu Jiang Seon-Hee Yim Hai-Dong Xu Seung-Hyun Jung So Young Yang Hae-Jin hu Chan-Kwon Jung Yeun-Jun Chung Integrated Research Center for Genome Polymorphism and Department of Microbi-ology School of Medicine The Catholic University of KoreaSocho-gu Seoul 137-701 South Korea Integrated Research Center for Genome Polymorphism and Department of Medical humanities and SocialSciences School of Medicine The Catholic University of Korea Socho-gu Seoul 137-701 South Korea Hospital Pathology Seoul St Mary's Hospi-tal Socho-gu Seoul 137-701 South Korea

AIM: To explore the expression pattern of E2F5 in primary hepatocellular carcinomas (HCCs) and elucidate the roles of E2F5 in hepatocarcinogenesis. METHODS: E2F5 expression was analyzed in 120 primary HCCs and 29 normal liver tissues by immunohistochemistry analysis. E2F5-small interfering RNA was transfected into HepG2, an E2F5-overexpressed HCC cell line. After E2F5 knockdown, cell growth capacity and migrating potential were examined. RESULTS: E2F5 was significantly overexpressed in primary HCCs compared with normal liver tissues (P = 0.008). The E2F5-silenced cells showed significantly reduced proliferation (P = 0.004). On the colony formation and soft agar assays, the number of colonies was significantly reduced in E2F5-silenced cells (P = 0.004 and P = 0.009, respectively). E2F5 knockdown resulted in the accumulation of G0/G1 phase cells and a reduction of S phase cells. The number of migrating/invading cells was also reduced after E2F5 knockdown (P = 0.021). CONCLUSION: To our knowledge, this is the first evidence that E2F5 is commonly overexpressed in primary HCC and that E2F5 knockdown significantly repressed the growth of HCC cells.

关键词： E2F5 E2F family Hepatocellular carcinoma Oncogene Small interfering RNA

EB病毒在原发性干燥综合征发病机制中的研究进展

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中华微生物学和免疫学杂志 2016年第9期36卷 712-715页

作者：何雨竹余毅恺胡绍先华中科技大学同济医学院附属同济医院风湿免疫科武汉430030

原发性干燥综合征（ primary Sj？gren′s syndrome, pSS）是一种慢性自身免疫性疾病,可累及全身多个系统,迄今pSS的具体发病机制仍未阐明。 EB病毒（ Epstein-Barr virus,EBV）也称人类Ⅳ型疱疹病毒,属于疱疹病毒属,研究发现EBV和pSS... 详细信息

原发性干燥综合征（ primary Sj？gren′s syndrome, pSS）是一种慢性自身免疫性疾病,可累及全身多个系统,迄今pSS的具体发病机制仍未阐明。 EB病毒（ Epstein-Barr virus,EBV）也称人类Ⅳ型疱疹病毒,属于疱疹病毒属,研究发现EBV和pSS的发病有关。随着研究的不断深入,关于EBV致pSS发病的新证据和新观点越来越多,其可能通过多种途径参与pSS发病。本文对pSS发病机制中EBV致病作用的相关研究进行综述。

关键词：干燥综合征 EB病毒干扰素淋巴瘤

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融合双层注意力机制的群组偏好融合策略研究

计算机工程与应用 2023年第9期59卷 272-279页

作者：梅雨竹胡竹林朱欣娟西安工程大学计算机科学学院西安710000 陕西省图书馆数字资源部西安710000

当前推荐系统研究热点及其演变趋势之一是个性化推荐由关注个体推荐逐步转向关注群体推荐。目前多数群组推荐方法在选择偏好融合策略时习惯采用预定义的静态策略,而静态策略的特点就导致算法无法最大化模拟出群组决策的真实过程。在前... 详细信息

当前推荐系统研究热点及其演变趋势之一是个性化推荐由关注个体推荐逐步转向关注群体推荐。目前多数群组推荐方法在选择偏好融合策略时习惯采用预定义的静态策略,而静态策略的特点就导致算法无法最大化模拟出群组决策的真实过程。在前人研究的基础之上提出一种基于双层注意力机制的群组推荐方法,该方法充分考虑到群体用户的差异性和相互影响,以及对于不同领域的决策权等问题。计算群组内每位成员对其他成员的注意力权重,获得群组成员特征向量,再计算每个成员在选择某一个项目的注意力权重,为群组生成对于该项目的偏好向量,以此来充分还原群组用户之间的交互以及群组决策的过程。通过在CAMRa2011和Meetup数据集上与COM、SIG、AGR、AGREE、FastGR等方法在不同参数条件下进行了对比,在归一化折扣累计增益和命中率两个指标上,相较基线模型平均提高了0.025 4和0.030 7。

关键词：群组推荐注意力机制偏好融合策略推荐算法

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藏绵羊DRB3基因第2外显子多态性分析

农业生物技术学报 2010年第5期18卷 925-930页

作者：成述儒罗玉柱胡江甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室

为了研究藏绵羊(Ovis aries)DRB3基因的多态性,确定其等位基因数、等位基因间的核苷酸多态位点、变异类型,以及分析各等位基因间的遗传关系和进化意义,研究采用PCR-SSCP方法检测了500只藏绵羊DRB3基因第2外显子的多态性,并克隆、测序群... 详细信息

为了研究藏绵羊(Ovis aries)DRB3基因的多态性,确定其等位基因数、等位基因间的核苷酸多态位点、变异类型,以及分析各等位基因间的遗传关系和进化意义,研究采用PCR-SSCP方法检测了500只藏绵羊DRB3基因第2外显子的多态性,并克隆、测序群体内变异产生的各等位基因。结果表明藏绵羊DRB3基因第2外显子表现了37个等位基因,37个单倍型序列分析发现82个核苷酸多态位点;37个单倍型序列与GenBank下载序列对比分析,结果表明35个DRB3的等位基因是本研究首次发现;37个DRB3基因外显子2的单倍型序列NJ系统发育树呈2支分化趋势。研究认为藏绵羊DRB3基因第2外显子具有丰富的遗传多态性;藏绵羊DRB3基因最初是由2个等位基因突变分化成两大类等位基因的。

关键词： DRB3基因多态性 PCR-SSCP 藏绵羊

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太极拳习练中“静”的身体哲学论绎

西安体育学院学报 2022年第5期39卷 590-596页

作者：胡浩赵倩金玉柱西安交通大学体育中心西安710049 西安电子科技大学终南山武术研究中心西安710071

运用诠释学方法,从身体哲学出发,深入剖析太极拳“静”的思想理念,挖掘与整理太极拳的身体经验及其价值取向。研究指出,太极拳的“静”主要包括“阴阳动静”之静、“体用动静”之静与“持敬主静”之静。太极拳习练中的“静”主要蕴含“... 详细信息

运用诠释学方法,从身体哲学出发,深入剖析太极拳“静”的思想理念,挖掘与整理太极拳的身体经验及其价值取向。研究指出,太极拳的“静”主要包括“阴阳动静”之静、“体用动静”之静与“持敬主静”之静。太极拳习练中的“静”主要蕴含“松静自然,以意领形”的身体感知,“心无杂念,引神入静”的身体意象,以及“练养结合,动静合一”的身体境界。太极拳习练中的“静”在价值取向上,追求“静而空欲”的行为秩序与身心养护,强调“静而平躁”的自我调适与德性养护,注重“静而缓急”的实践智慧与精神养护,坚守“静合天人”的宇宙意识与生命养护。太极拳中的“静”既在心,又在身;既内在,又超越;既感性,又超感性,可视为中国式的“即身而道在”的形而上学。

关键词：太极拳 “静” 身体哲学价值取向

Variation in the caprine keratin-associated protein 15-1（KAP15-1） gene affects cashmere fibre diameter

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Variation in the caprine keratin-associated protein 15-1（KA...

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Mengli Zhao huitong Zhou Jon G.H.Hickford Jiqing Wang Jiang hu Xiu Liu Shaobin Li yuzhu Luo Faculty of Animal Science and Technology Gansu Key Laboratory of Herbivorous Animal BiotechnologyGansu Agricultural University

Keratin-associated proteins（KAPs） are a structural component of cashmere fibre, and variation in some KAP genes（KRTAPs） has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism（PCR-SSCP） technique in 250 Longdong goats, and six variants（named A to F） containing eight single nucleotide polymorphisms（SNPs）, were identified. Five of the SNPs were non-synonymous, and would lead to putative amino acid changes. Reverse transcription-polymerase chain reaction（RT-PCR） analysis revealed that KRTAP15-1 was expressed in secondary hair follicles, but not in heart tissue, liver tissue, lung tissue, kidney tissue, or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine, and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter（MFD）, and this effect appeared to be dominant;while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.

关键词： Keratin-associated protein KAP15-1 KRTAP15-1 cashmere mean fibre diameter tissue expression goat

The Preliminary Research on Rupture and Factors Affecting Rupturing of the Hoh Sai hu Segment in Eastern Kunlun

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Earthquake Research in China 2012年第4期26卷 444-456页

作者： Bai yuzhu Xu Jie Zhou Qing Zhou Bengang Key Laboratory of Active Tectonics and Volcano Institute of Geology CEA

On November 14, 2001, an extraordinarily large earthquake (MS8.1) occurred on the Hoh Sai hu segment of the Eastern Kunlun Fault, in the northern Qinghai-Tibetan Plateau. The seismogenic fault, the Hoh Sai hu segment, is a left-lateral fault with a high slip rate in geological history, with the average slip rate reaching(14.8±2.8)mm/a since the late Pleistocene. Different slip rates of the Hoh Sai hu segment can affect fault motion in the future. Therefore, this paper analyzes the effect of different slip rates and different initial friction coefficients on the fault plane of the Hoh Sai hu segment of the eastern Kunlun Fault on the rupture behaviors of the fault. In this research, we apply the single degree of spring block model controlled by the rateand state-dependent frictional constitutive laws. Using the fault dislocation model and based on ancient earthquake research, historical earthquakes data and the achievements of previous researchers, we obtained the parameters of this model. Through numerical simulation of the rupturing motion of the Hoh Sai hu segment in the next 6500 years under different slip rates, we find that a faster annual slip rate will shorten the recurrence interval of the earthquake. For example, the earthquake recurrence interval is 2100a at a slip rate of 0.014m/a, which agrees with previous research, but, the recurrence interval will be 1000~1500a and 2100～2500a, corresponding to the slip rates of 0.018m/a and 0.008m/a, respectively. The fault slip rate has no regular effect on the coseismic slip rate and fault displacement in an earthquake. The initial friction coefficient on the fault surface has an effect on the earthquake recurrence interval. A smaller initial friction coefficient will lengthen the earthquake recurrence interval. At the same time, the smaller initial friction coefficient will lead to larger slip rates and fault displacement when earthquakes occur.

关键词： Fault Spring-block model Rupture behavior Earthquake recurrenceinterval