A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma

作     者：Yuzhu Jiang Seon-Hee Yim Hai-Dong Xu Seung-Hyun Jung So Young Yang Hae-Jin Hu Chan-Kwon Jung Yeun-Jun Chung 

作者机构：Integrated Research Center for Genome Polymorphism and Department of Microbi-ology School of Medicine The Catholic University of KoreaSocho-gu Seoul 137-701 South Korea Integrated Research Center for Genome Polymorphism and Department of Medical Humanities and SocialSciences School of Medicine The Catholic University of Korea Socho-gu Seoul 137-701 South Korea Hospital Pathology Seoul St Mary's Hospi-tal Socho-gu Seoul 137-701 South Korea 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2011年第17卷第4期

页      面：470-477页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by A grant of the Korea Healthcare technology R&D Project, Ministry of Health and Welfare, Republic of Korea (A092258) FG08-11-06 of the 21C Frontier Functional Human Genome Project from the Ministry of Education, Science and Technology 

主　　题：E2F5 E2F family Hepatocellular carcinoma Oncogene Small interfering RNA 

摘      要：AIM: To explore the expression pattern of E2F5 in primary hepatocellular carcinomas (HCCs) and elucidate the roles of E2F5 in hepatocarcinogenesis. METHODS: E2F5 expression was analyzed in 120 primary HCCs and 29 normal liver tissues by immunohistochemistry analysis. E2F5-small interfering RNA was transfected into HepG2, an E2F5-overexpressed HCC cell line. After E2F5 knockdown, cell growth capacity and migrating potential were examined. RESULTS: E2F5 was significantly overexpressed in primary HCCs compared with normal liver tissues (P = 0.008). The E2F5-silenced cells showed significantly reduced proliferation (P = 0.004). On the colony formation and soft agar assays, the number of colonies was significantly reduced in E2F5-silenced cells (P = 0.004 and P = 0.009, respectively). E2F5 knockdown resulted in the accumulation of G0/G1 phase cells and a reduction of S phase cells. The number of migrating/invading cells was also reduced after E2F5 knockdown (P = 0.021). CONCLUSION: To our knowledge, this is the first evidence that E2F5 is commonly overexpressed in primary HCC and that E2F5 knockdown significantly repressed the growth of HCC cells.