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中国病毒学 2007年第1期22卷 61-67页

作者： qing-yu cheng Xiao-lin MENG Jin-ping XU Wei LU Jian WANG State Key Laboratory of Virology College of Life Science Wuhan University Wuhan 430072 People＇s Republic of China

我们为 WSSV 开发了敏感、快速的侧面流动的免疫分析(LFIA ) ，用象指示物的胶体的黄金。熔化蛋白质， VP (19+28 ) ，在 E 被表示。coli，净化并且过去常准备 polyclonal 抗体。净化的 anti-VP (19+28 ) IgG 与胶体的黄金被结合。Uncon... 详细信息

我们为 WSSV 开发了敏感、快速的侧面流动的免疫分析(LFIA ) ，用象指示物的胶体的黄金。熔化蛋白质， VP (19+28 ) ，在 E 被表示。coli，净化并且过去常准备 polyclonal 抗体。净化的 anti-VP (19+28 ) IgG 与胶体的黄金被结合。Unconjugated anti-VP (19+28 ) IgG 和山羊反兔子 IgG 在硝化纤维素膜上被使不能调动。在汇编以后，虾样品的三个组(在每个组的 5 个单个动物) 被测试它包括了健康、垂死、死了的虾。为每个组，三不同纸巾(身体蜜汁，鳃和 hepatopancreas ) 同时被测试。在平行，所有样品也为比较用 PCR 被分析。从测试的 45 件样品，当 15 作为否定被分类时， 30 作为积极被检测。LFIA 的结果相关，那些由 PCR 分析获得了，显示这二个察觉方法有在在这初步的研究测试的样品的有限数字的一样的功效。关键词侧面流动的免疫分析 - 白点症候群病毒(WSSV )- VP19 - VP28 CLC 数字 S945.

关键词： Lateral-flow immunoassay White spot syndrome virus (WSSV) VP19 VP28

Early adenocarcinoma mixed with a neuroendocrine carcinoma component arising in the gastroesophageal junction: A case report

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 563-570页

作者： yu-qing cheng Geng-Fang Wang Xiao-Li Zhou Min Lin Xin-Wen Zhang Qin Huang Department of Pathology The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityChangzhou 213164Jiangsu ProvinceChina Gastroenterology Center The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityChangzhou 213164Jiangsu ProvinceChina Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Department of Pathology Beth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMA 02215United States

BACKGROUND Early adenocarcinoma mixed with a neuroendocrine carcinoma(NEC)component arising in the gastroesophageal junctional(GEJ)region is rare and even rarer in young ***,we report such a case in a 29-year-old Chinese *** SUMMARY This patient presented to our hospital with a 3-mo history of dysphagia and *** endoscopy revealed an elevated nodule in the distal esophagus 1.6 cm above the GEJ line,without Barrett’s esophagus or involvement of the gastric *** nodule was completely resected by endoscopic submu-cosal dissection(ESD).Pathological examination confirmed diagnosis of intra-mucosal adenocarcinoma mixed with an NEC component,measuring 1.5 ***,both adenocarcinoma and NEC components were positive for P53 with a Ki67 index of 90%;NEC was positive for synaptophysin and ***-generation sequencing of 196 genes demonstrated a novel germline mutation of the ERCC3 gene in the DNA repair pathway and a germline mutation of the RNF43 gene,a common gastric cancer driver gene,in addition to pathogenic somatic mutations in P53 and CHEK2 *** patient was alive without evidence of the disease 36 mo after *** Early adenocarcinoma with an NEC component arising in the distal esophageal side of the GEJ region showed evidence of gastric origin.

关键词： Adenocarcinoma Neuroendocrine carcinoma Gastroesophageal junction Next generation sequencing Case report

Therapeutic Effect of Prolyl Endopeptidase Inhibitor in High-fat Diet-induced Metabolic Dysfunction-associated Fatty Liver Disease

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Journal of Clinical and Translational Hepatology 2023年第5期11卷 1035-1049页

作者： Jian-Bin Zhang Meng-Ting Li Shuang-Zhe Lin yu-qing cheng Jian-Gao Fan yuan-Wen Chen Department of Gastroenterology Xinhua HospitalShanghai Jiaotong University School of MedicineShanghaiChina Department of Gastroenterology Huadong HospitalFudan UniversityShanghaiChina Department of Gastroenterology The Affiliated People's Hospital of Ningbo UniversityNingboZhejiangChina Department of Geriatrics Huadong HospitalFudan UniversityShanghaiChina

Background and Aims:Prolyl endopeptidase(PREP)is a serine endopeptidase that participates in many pathological processes including inflammation,oxidative stress,and *** previous studies found that PREP knockout exhibited multiple benefits in high-fat diet(HFD)or methionine choline-deficient diet-induced metabolic dysfunctionassociated fatty liver disease(MAFLD).However,cumulative studies have suggested that PREP performs complex functions during disease ***,further understanding the role of PREP in MAFLD development is the foundation of PREP ***:In this study,an HFD-induced MAFLD model at different time points(4,8,12,and 16 weeks)was used to explore dynamic changes in the PREP proline-glycine-proline(PGP)/N-acetyl-seryl-aspartyllysyl-proline(AcSDKP)*** explore its potential value in MAFLD treatment,saline,or the PREP inhibitor,KYP-2047,was administered to HFD-induced MAFLD mice from the 10th to 16th ***:PREP activity and expression were increased in HFD-mice compared with control mice from the 12th week onwards,and increased PREP mainly resulted in the activation of the matrix metalloproteinase 8/9(MMP8/9)-PREP-PGP axis rather than the thymosin B4-meprin a/PREP-AcSDKP *** addition,KYP-2047 reduced HFD-induced liver injury and oxidative stress,improved lipid metabolism through the suppression of lipogenic genes and the induction of B-oxidation-related genes,and attenuated hepatic inflam-mation by decreasing MMP8/9 and ***,KYP2047 restored HFD-induced impaired autophagy and this was veri-fied in HepG2 ***:These findings suggest that increased PREP activity/expression during MAFLD de-velopment might be a key factor in the transition from sim-ple steatosis to steatohepatitis,and KYP-2047 might possess therapeutic potential for MAFLD treatment.

关键词： Metabolic dysfunction-associated fatty liver disease Prolyl endopeptidase KYP-2047 Proline-glycine-proline N-acetyl-seryl-aspartyl-lysylproline

Establishing a rabbit model of perianal fistulizing Crohn's disease

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World Journal of Gastroenterology 2022年第15期28卷 1536-1547页

作者： Shuang-Shuang Lu Wen-Jia Liu Qiu-Ya Niu Chun-Yan Huo yu-qing cheng En-Jing Wang Rong-Nan Li Fang-Fang Feng Yi-Ming cheng Rong Liu Jin Huang Gastroenterology Center The Affiliated Changzhou Second People's Hospital of Nanjing Medical UniversityChangzhou 213000Jiangsu ProvinceChina Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Graduate School Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Medical Statistics Center Changzhou UniversityChangzhou 213000Jiangsu ProvinceChina

BACKGROUND Crohn’s disease(CD)is a chronic nonspecific intestinal inflammatory *** aetiology and pathogenesis of CD are still *** fistula is the main complication of CD and is a difficult problem to solve at *** main limitation of developing new therapies is bound up with the short of preclinical security and effectiveness ***,an ideal animal model is needed to establish persistent anal fistula and an inflamed rectal *** To improve the induction method of colitis and establish a reliable and reproducible perianal fistulizing Crohn’s disease animal model to evaluate new treatment *** Twenty male New Zealand rabbits underwent rectal enema with different doses of 2,4,6-trinitrobenzene sulfonic acid to induce *** A was treated with an improved equal interval small dose increasing *** dosage of group B was *** days later,the rabbits underwent surgical creation of a transsphincteric ***,three rabbits were randomly selected from each group every 7 d to remove the seton from the *** rabbits were examined by endoscopy every 7 days,and biopsy forceps were used to obtain tissue samples from the obvious colon lesions for histological *** disease activity index(DAI),colonoscopy and histological scores were *** endoscopic ultrasonography(EUS)was used to evaluate the healing of *** Except for the DAI score,the colonoscopy and histological scores in group A were significantly higher than those in group B(P<0.05).In the ideal model rabbit group,on the 7 th day after the removal of the seton,all animals had persistent lumens on EUS imaging,showing continuous fullthickness high *** inspection of the fistula showed acute and chronic inflammation,fibrosis,epithelialization and peripheral proctitis of the adjoining *** The improved method of CD colitis induction successfully established a rabbit perianal fistula CD pre

关键词： Crohn’s disease Perianal fistula Model Endoscopy Histology

Hourly traffic flow forecasting using a new hybrid modelling method

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Journal of Central South University 2022年第4期29卷 1389-1402页

作者： LIU Hui ZHANG Xin-yu YANG yu-xiang LI Yan-fei yu cheng-qing Institute of Artificial Intelligence and Robotics(IAIR) Key Laboratory of Traffic Safety on Track of Ministry of EducationSchool of Traffic and Transportation EngineeringCentral South UniversityChangsha 410075China School of Mechatronic Engineering Hunan Agricultural UniversityChangsha 410128China

Short-term traffic flow forecasting is a significant part of intelligent transportation *** some traffic control scenarios,obtaining future traffic flow in advance is conducive to highway management department to have sufficient time to formulate corresponding traffic flow control *** hence,it is meaningful to establish an accurate short-term traffic flow method and provide reference for peak traffic flow *** paper proposed a new hybrid model for traffic flow forecasting,which is composed of the variational mode decomposition(VMD)method,the group method of data handling(GMDH)neural network,bi-directional long and short term memory(BILSTM)network and ELMAN network,and is optimized by the imperialist competitive algorithm(ICA)*** illustrate the performance of the proposed model,there are several comparative experiments between the proposed model and other *** experiment results show that 1)BILSTM network,GMDH network and ELMAN network have better predictive performance than other single models;2)VMD can significantly improve the predictive performance of the ICA-GMDH-BILSTM-ELMAN *** effect of VMD method is better than that of EEMD method and FEEMD *** conclude,the proposed model which is made up of the VMD method,the ICA method,the BILSTM network,the GMDH network and the ELMAN network has excellent predictive ability for traffic flow series.

关键词： traffic flow forecasting intelligent transportation system imperialist competitive algorithm variational mode decomposition group method of data handling bi-directional long and short term memory ELMAN

Relationship between longitudinal changes in lipid composition and ischemic stroke among hypertensive patients

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World Journal of Clinical Cases 2025年第4期13卷 18-27页

作者： cheng-cheng Wei yu-qing Huang cheng-Hong yu Department of Cardiology Tongxiang First People's HospitalTongxiang 314500Zhejiang ProvinceChina Department of Cardiology Guangdong Cardiovascular InstituteGuangzhou 510080Guangdong ProvinceChina

BACKGROUND Dyslipidemia was strongly linked to stroke,however the relationship between dyslipidemia and its components and ischemic stroke remained *** To investigate the link between longitudinal changes in lipid profiles and dyslipidemia and ischemic stroke in a hypertensive *** Between 2013 and 2014,6094 hypertension individuals were included in this,and ischemic stroke cases were documented to the end of *** changes of lipid were stratified into four groups:(1)Normal was transformed into normal group;(2)Abnormal was transformed into normal group;(3)Normal was transformed into abnormal group;and(4)Abnormal was transformed into abnormal *** examine the link between longitudinal changes in dyslipidemia along with its components and the risk of ischemic stroke,we utilized multivariate Cox proportional hazards models with hazard ratio(HR)and 95%*** The average age of the participants was 62.32 years±13.00 years,with 329 women making up 54.0%of the *** the course of a mean follow-up of 4.8 years,143 ischemic strokes *** normal was transformed into normal group was used as a reference,after full adjustments,the HR for dyslipidemia and ischemic stroke among abnormal was transformed into normal group,normal was transformed into abnormal group and abnormal was transformed into abnormal Wei CC et *** changed and ischemic stroke WJCC https://*** 2 February 6,2025 Volume 13 Issue 4 group were 1.089(95%CI:0.598-1.982;P=0.779),2.369(95%CI:1.424-3.941;P<0.001)and 1.448(95%CI:1.002-2.298;P=0.047)(P for trend was 0.233),*** In individuals with hypertension,longitudinal shifts from normal to abnormal in dyslipidemia-particularly in total and low-density lipoprotein cholesterol-were significantly associated with the risk of ischemic stroke.

关键词： Longitudinal change Hypertension Dyslipidemia Lipid profile Ischemic stroke

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一种基于改进主成分分析的人脸识别方法

激光与红外 2007年第5期37卷 478-480页

作者：苑玮琦于清澄沈阳工业大学视觉检测技术研究室辽宁沈阳110023

提出一种主成分分析(PCA)和遗传算法(GA)相结合的人脸识别方法。利用遗传算法进行特征矢量的选择,以构成最有利于分类的特征空间。通过对ORL和Yale两个人脸数据库的实验结果,表明PCA和GA相结合的人脸识别算法优于传统的PCA人脸识别算法。

关键词：人脸识别主成分分析遗传算法

Preparation of FeCoNi medium entropy alloy from Fe^(3+)-Co^(2+)-Ni^(2+)solution system

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 92-101页

作者： Zongyou cheng qing Zhao Mengjie Tao Jijun Du Xingxi Huang chengjun Liu Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China Engineering Research Center of Frontier Technologies for Low-carbon Steelmaking(Ministry of Education) Shenyang 110819China

In recent years,medium entropy alloys have become a research hotspot due to their excellent physical and chemical *** controlling reasonable elemental composition and processing parameters,the medium entropy alloys can exhibit similar properties to high entropy alloys and have lower *** this paper,a FeCoNi medium entropy alloy precursor was prepared via sol-gel and coprecipitation methods,respectively,and FeCoNi medium entropy alloys were prepared by carbothermal and hydrogen *** phases and magnetic properties of FeCoNi medium entropy alloy were *** showed that FeCoNi medium entropy alloy was produced by carbothermal and hydrogen reduction at 1500℃.Some carbon was detected in the FeCoNi medium entropy alloy prepared by carbothermal *** alloy prepared by hydrogen reduction was uniform and showed a relatively high ***,the hydrogen reduction product exhibited better saturation magnetization and lower coercivity.

关键词： medium entropy alloy sol-gel co-precipitation carbothermal hydrogen reduction

青海西台吉乃尔湖盐田蒸发光卤石矿的粒度分布及其矿物学特征

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矿物岩石 2016年第4期36卷 16-22页

作者：海擎宇程怀德马海州中国科学院青海盐湖研究所青海西宁810008 青海省盐湖地质与环境重点实验室青海西宁810008 中国科学院大学北京100049

以煤油介质,利用筛分法对青海西台吉乃尔盐湖盐田蒸发光卤石原矿进行粒度分级,通过X射线衍射方法确定各粒级颗粒矿物组成,运用扫描电镜表征细粒盐类晶体微观形貌特征,结合化学分析结果,揭示原矿粒度分布规律和矿物组成变化特征。结果表... 详细信息

以煤油介质,利用筛分法对青海西台吉乃尔盐湖盐田蒸发光卤石原矿进行粒度分级,通过X射线衍射方法确定各粒级颗粒矿物组成,运用扫描电镜表征细粒盐类晶体微观形貌特征,结合化学分析结果,揭示原矿粒度分布规律和矿物组成变化特征。结果表明:光卤石原矿粒度分布属于中细较粗粒级,大于1mm粒径的矿物比例不超过50%,而小于0.5mm粒径的矿物不足10%;各粒径矿物组分有变化,光卤石主要集中在较细粒级范围(0~1mm),泻利盐在各个粒级范围内均有出现,粒度越大,泻利盐的含量越大,氯化钠主要富集在中粒级范围(1mm^3mm),水氯镁石在各粒径均有分布,无明显变化。小于0.45mm粒径矿物SEM和能谱分析结果表明:光卤石表面圆润,存在脱水的可能性;石盐和泻利盐、光卤石和泻利盐团聚在一起,形成胶结状聚集体。光卤石原矿的粒度分布特征和矿物微结构特征影响分解转化过程体系的水平衡和溶液相平衡,对氯化钾收率产生影响。

关键词：光卤石泻利盐筛分法扫描电镜

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基于现场总线技术的工业控制系统研究

价值工程 2014年第1期33卷 68-69页

作者：于清澄中油辽河工程有限公司自控仪表所盘锦124010

现场总线是现代工业控制技术的发展方向,对于现场总线来说,其特点是现场通信、现场设备互联,具有较好的互操作性,并且功能模块分散化,同时具有开放式互联网等。本文通过对现场总线技术,以及通信协议进行仔细分析和研究,对几种常用的现... 详细信息

现场总线是现代工业控制技术的发展方向,对于现场总线来说,其特点是现场通信、现场设备互联,具有较好的互操作性,并且功能模块分散化,同时具有开放式互联网等。本文通过对现场总线技术,以及通信协议进行仔细分析和研究,对几种常用的现场总线进行比较,说明了由其构成的工业控制系统的结构,最后针对CAN总线设计了一种工业控制系统并强调了在设计中所需注意的问题。

关键词：现场总线工业控制系统通信协议 CAN总线