Establishing a rabbit model of perianal fistulizing Crohn's disease
作者机构：Gastroenterology CenterThe Affiliated Changzhou Second People's Hospital of Nanjing Medical UniversityChangzhou 213000Jiangsu ProvinceChina Graduate SchoolDalian Medical UniversityDalian 116044Liaoning ProvinceChina Graduate SchoolNanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Medical Statistics CenterChangzhou UniversityChangzhou 213000Jiangsu ProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2022年第28卷第15期
页 面：1536-1547页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
主 题：Crohn’s disease Perianal fistula Model Endoscopy Histology
摘 要：BACKGROUND Crohn’s disease(CD)is a chronic nonspecific intestinal inflammatory *** aetiology and pathogenesis of CD are still *** fistula is the main complication of CD and is a difficult problem to solve at *** main limitation of developing new therapies is bound up with the short of preclinical security and effectiveness ***,an ideal animal model is needed to establish persistent anal fistula and an inflamed rectal *** To improve the induction method of colitis and establish a reliable and reproducible perianal fistulizing Crohn’s disease animal model to evaluate new treatment *** Twenty male New Zealand rabbits underwent rectal enema with different doses of 2,4,6-trinitrobenzene sulfonic acid to induce *** A was treated with an improved equal interval small dose increasing *** dosage of group B was *** days later,the rabbits underwent surgical creation of a transsphincteric ***,three rabbits were randomly selected from each group every 7 d to remove the seton from the *** rabbits were examined by endoscopy every 7 days,and biopsy forceps were used to obtain tissue samples from the obvious colon lesions for histological *** disease activity index(DAI),colonoscopy and histological scores were *** endoscopic ultrasonography(EUS)was used to evaluate the healing of *** Except for the DAI score,the colonoscopy and histological scores in group A were significantly higher than those in group B(P0.05).In the ideal model rabbit group,on the 7 th day after the removal of the seton,all animals had persistent lumens on EUS imaging,showing continuous fullthickness high *** inspection of the fistula showed acute and chronic inflammation,fibrosis,epithelialization and peripheral proctitis of the adjoining *** The improved method of CD colitis induction successfully established a rabbit perianal fistula CD pre
维普期刊数据库