检索结果-南通市图书馆

Ethanol extracts of Hizikia fusiforme induce apoptosis in human prostate cancer PC3 cells via modulating a ROS-dependent pathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2020年第2期10卷 78-86页

作者： Eun Ok choi Hyesook Lee Cheol Park Gi-young Kim Hee-Jae Cha Suhkmann Kim Heui-Soo Kim You-jin Jeon Hye jin Hwang Yung Hyun choi Anti-Aging Research Center Dong-eui UniversityBusan 47340Republic of Korea Department of Biochemistry College of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea Department of Molecular Biology College of Natural SciencesDong-eui UniversityBusan 47340Republic of Korea Laboratory of Immunobiology Department of Marine Life SciencesJeju National UniversityJeju 63243Republic of Korea Department of Parasitology and Genetics Kosin University College of MedicineBusan 49267Republic of Korea Department of Chemistry College of Natural SciencesCenter for Proteome Biophysics and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241Republic of Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Food and Nutrition College of NursingHealthcare Sciences&Human EcologyDong-eui UniversityBusan 47340Republic of Korea

Objective:To investigate whether ethanol extracts of Hizikia fusiforme could induce apoptosis in human prostate cancer PC3 ***:Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium *** and mitochondrial membrane potential(MMP)were measured using flow cytometry in PC3 *** damage was assessed by nuclear staining and DNA fragmentation *** of apoptosis-associated proteins were determined by Western blotting *** of caspase-3,-8,and-9 were determined by colorimetric ***,intracellular reactive oxygen species(ROS)generation was detected using a flow cytometer and fluorescence ***:Treatment of PC3 cells with ethanol extracts of Hizikia fusiforme inhibited proliferation,which was associated with induction of apoptosis,and accompanied by increased expression of Fas,Fas-ligand(Fas L),Bax and t Bid,and decreased expression of *** addition,ethanol extracts of Hizikia fusiforme reduced c-Flip expression and activated caspase-8,-9 and-3,resulting in an increase in poly(ADP-ribose)polymerase(PARP)***,in the presence of a pan-caspase inhibitor,ethanol extracts of Hizikia fusiforme-mediated growth inhibition and apoptosis were significantly *** extracts of Hizikia fusiforme also destroyed the integrity of mitochondria due to the loss of MMP,leading to cytosolic release of cytochrome ***,the levels of ROS were markedly increased by treatment with ethanol extracts of Hizikia fusiforme,which was significantly suppressed by the ROS scavenger *** investigation of whether ethanol extracts of Hizikia fusiforme-induced apoptosis was related to the generation of ROS was conducted and the results showed that N-acetyl-L-cysteine fully blocked ethanol extracts of Hizikia fusiforme-induced apoptotic events including loss of MMP,activation of caspase-3,the cytosolic release of cytochrome c and ***:E

关键词： Hizikia fusiforme Apoptosis Prostate cancer cells Caspase Reactive oxygen species

A model and simulation of cathode flooding and drying on unsteady proton exchange membrane fuel cell

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Central South University 2012年第9期19卷 2572-2577页

作者： A.Bakhtiar KIM young-Bok YOU jin-Kwang YOON Jung-In choi Kwang-Hwan Refrigeration and Air-Conditioning Engineering Pukyong National University Department of Automotive Dong-Pusan Collage University Department of Mechanical Engineering Pukyong National University

A water balance has a significant impact on the overall system performance in proton exchange membrane fuel *** actual fuel cell application has a dynamic electrical load which means also dynamic electrical ***,since this electrical current is known,the water production from the fuel cell reaction is also able to be *** long as the fuel cell water transportation model is provided,the present liquid water inside the porous medium is also able to be modeled.A model of the liquid water saturation level in a fuel cell in unsteady load condition was *** model is a series of the water transportation model of water saturation level for the final output of proton exchange membrane(PEM) fuel cell to predict the flooding or drying of PEM fuel *** simulation of vehicle fuel cell in different dynamic load profiles and different inlet air conditions was done using this *** simulation result shows that PEM fuel cell with different dynamic load profiles has different liquid water saturation level *** means that a dynamic load fuel cell requires also a dynamic input air humidification.

关键词： model simulation flooding unsteady condition fuel cell

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2019年第1期12卷 14-18页

作者： Ryong Gong Dae young Lee Jae Won Lee Doo jin choi Geum-Soog Kim Sang Hyuk Lee young-Seob Lee Department of Herbal Crop Research National Institute of Horticultural & Herbal Science Rural Development Administration

Objective: To investigate the synergistic effect between rhein(RHE) and oxacillin against Staphylococcus aureus(MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.

关键词： Antimicrobials Mechanism of action Staphylococcus aureus Polymerase chain reaction

The beneficial impacts of splanchnic vasoactive agents on hepatic functional recovery in massive hepatectomy porcine model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary Surgery and Nutrition 2021年第3期10卷 325-336页

作者： Hye-Sung Jo Jae Hyun Han Yoon young choi jin-I Seok young-In Yoon Dong-Sik Kim Division of HBP Surgery and Liver Transplantation Department of SurgeryKorea University College of MedicineSeoulRepublic of Korea Department of Surgery St.Vincent’s HospitalCollege of MedicineThe Catholic University of KoreaSeoulRepublic of Korea Department of Biomedical Science Korea University College of Medicine Graduate SchoolSeoulRepublic of Korea Division of Hepatobiliary Surgery and Liver Transplantation Department of SurgeryAsan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea

Background:Excessive portal pressure after massive hepatectomy can cause hepatic sinusoidal injury and have deleterious impacts on hepatic functional recovery,contributing to developing post-hepatectomy liver *** study aimed to assess the effects of splanchnic vasoactive agents on hepatic functional recovery and regeneration while clarifying the underlying mechanism,using a 70%hepatectomy porcine ***:Eighteen pigs undergoing 70%hepatectomy were involved in this study and divided into three groups:control(n=6),terlipressin(n=6),and octreotide(n=6).Terlipressin(0.5 mg)and octreotide(0.2 mg)were administered 3 times a day for each group with the first dose starting just before surgery until the 7th postoperative day,at which time the surviving pigs were *** the period,portal pressure,liver weight,biochemical analysis,histological injury score,and molecular markers were evaluated and compared between ***:The 7-day survival rates in the octreotide,terlipressin,and control groups were 100%,83.3%,and 66.7%,*** portal pressures decreased in both terlipressin and octreotide groups than the control group at 30 minutes,1 hour and 6 hours after *** amount of regeneration measured by liver weight to body weight ratio at the time of sacrifice in the terlipressin group was smaller than that in the control group(117%vs.129%,P=0.03).Serum aspartate aminotransferase(AST)and total bilirubin levels at 1 and 6 hours after hepatectomy and prothrombin time/international normalized ratio(PT/INR)at 6 hours after hepatectomy were significantly improved in the terlipressin and octreotide groups compared to the control *** endothelin-1(ET-1)was significantly lower in the terlipressin group than that in the control group 6 hours after hepatectomy(P<0.01).The histological injury score in the control group was significantly higher than that in the terlipressin group on the 7th postoperative day(P<0.01).Conclusions:Splanc

关键词： Hepatectomy portal pressure terlipressin somatostatin

Soil texture affects the conversion factor of electrical conductivity from 1:5 soil-water to saturated paste extracts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2022年第6期32卷 905-915页

作者： Bo-Seong SEO young-Jae JEONG Nu-Ri BAEK Hyun-jin PARK Hye In YANG Se-In PARK Woo-Jung choi Department of Rural and Bio-systems Engineering(BK21) Chonnam National UniversityGwangju 61186(Republic of Korea) Department of Renewable Resources University of AlbertaEdmontonAlberta T6G 2E3(Canada) Max Planck Institute for Biogeochemistry Jena 07745(Germany) National Institute of Environmental Research Incheon 22689(Republic of Korea) AgriBio Institute of Climate Change Management Chonnam National UniversityGwangju 61186(Republic of Korea)

Electrical conductivity(EC)of soil-water extracts is commonly used to assess soil ***,its conversion to the EC of saturated soil paste extracts(ECe),the standard measure of soil salinity,is currently required for practical *** many regression models can be used to obtain ECe from the EC of soil-water extracts,the application of a site-specific model to different sites is not straightforward due to confounding soil factors such as soil *** study was conducted to develop a universal regression model to estimate a conversion factor(CF)for predicting EC_(e) from EC of soil-water extracts at a 1:5 ratio(EC_(1:5)),by employing a site-specific soil texture(i.e.,sand content).A regression model,CF=8.9105e^(0.0106sand)/1.2984(r^(2)=0.97,P<0.001),was developed based on the results of coastal saline soil surveys(n=173)and laboratory experiments using artificial saline soils with different textures(n=6,sand content=10%-65%)and salinity levels(n=7,salinity=1-24 dS m^(-1)).Model performance was validated using an independent dataset and demonstrated that EC_(e) prediction using the developed model is more suitable for highly saline soils than for low saline *** feasibility of the regression model should be tested at other *** soil factors affecting EC conversion factor also need to be explored to revise and improve the model through further studies.

关键词： coastal saline soils electrical conductivity prediction reclaimed tideland regression model sand content saturation percentage soil salinity

Torsional vibration analysis of lathe spindle system with unbalanced workpiece

在线全文

学校读者我要写书评

暂无评论

Journal of Central South University 2011年第1期18卷 171-176页

作者：郭瑞 JANG Sung-Hyun choi young-Hyu School of Mechatronics Changwon National University

For the purpose of analyzing the torsional vibration caused by the gravitational unbalance torque arisen in a spindle system when it is machining heavy work piece,a 10-DOF lumped parameter model was made for the machine tool spindle system with geared *** using the elementary method and Runge-Kutta method in Matlab,the eigenvalue problem was solved and the pure torsional vibration responses were obtained and *** results show that the spindle system cannot operate in the desired constant rotating speed as far as the gravitational unbalance torque is engaged,so it may cause bad effect on machining *** the torsional vibration increases infinitely near the resonant frequencies,so the spindle system cannot operate normally during these spindle speed ranges.

关键词： unbalanced work piece gravitational unbalance torque torsional vibration spindle system geared transmission

Safety of endoscopic ultrasound-guided ethanol ablation for pancreatic cystic lesions: A single-center experience of 214 patients

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2019年第6期18卷 562-568页

作者： jin Ho choi Sang Hyub Lee young Hoon choi Min Su You Bang-Sup Shin Woo Hyun Paik Ji Kon Ryu Yong-Tae Kim Department of Internal Medicine Liver Research Institute Seoul National University College of Medicine

Background:Endoscopic ultrasound-guided ethanol ablation(EUS-EA)for pancreatic cystic lesions(PCLs)has been used in recent years as a feasible treatment modality for low malignant probability PCLs or patients considered high-risk for *** present study aimed to confirm the safety of EUS-EA and to find predictive factors for adverse event(AE).Methods:A retrospective review was performed from the prospectively maintained database of patients who underwent EUS-EA for PCLs from June 2006 to April 2018 at Seoul National University *** primary outcomes of the study were the rates of AEs and severe AEs by *** secondary outcome was the predictive factors of AEs including acute pancreatitis and abdominal ***:A total of 214 patients were evaluated and the diagnoses of PCLs according to cystic fluid analysis and clinical features were as follows:serous cystic neoplasm(32.2%),mucinous cystic neoplasm(26.6%),branch duct type intraductal papillary mucinous neoplasm(BD-IPMN)(29.4%),and pseudocyst(11.7%).Three patients(1.4%)experienced severe ***,AEs occurred in 71(33.2%)***-IPMN(OR:2.87;95%CI:1.05–7.84;P=0.040),multilocular cysts(OR:3.59;95%CI:1.09–11.85;P=0.036),suspected ethanol leakage during procedure(OR:10.68;95%CI:1.98–57.53;P=0.006),and sticky cystic fluid(OR:3.83;95%CI:1.20–12.24;P=0.024)were predictive factors for post-procedural acute *** of uncinate process(OR:2.99;95%CI:1.22–7.35;P=0.017)and PCLs with exophytic portion(OR:3.70;95%CI:1.96–7.01;P<0.001)were predictive factors for post-procedural abdominal ***:EUS-EA is a safe procedure with a very low rate of severe *** seems possible to predict the AEs according to the features of the procedure and PCLs.

关键词： Pancreatic cyst EUS-guided ethanol ablation Adverse events

Effect of trapping vascular endothelial growth factor-A in a murine model of dry eye with inflammatory neovascularization

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第11期9卷 1541-1548页

作者： jin Woo Kwon jin A choi Eun young Shin Tae Yoon La Dong Hyun Jee Yeon Woong Chung Yang Kyung Cho Department of Ophthalmology St.Vincent's HospitalCollege of Medicinethe Catholic University of Korea Research Institute of Medical Science St.Vincent's Hospitalthe Catholic University of Korea

AIM： To evaluate whether trapping vascular endothelial growth factor-A（VEGF-A） would suppress angiogenesis and inflammation in dry eye corneas in a murine corneal suture ***： We established two groups of animals, one with non-dry eyes and the other with induced dry *** both groups, a corneal suture model was used to induce inflammation and neovascularization. Each of two groups was again divided into three subgroups according to the treatment; subgroup I（aflibercept）,subgroup II（dexamethasone） and subgroup III（phosphate buffered saline, PBS）. Corneas were harvested and immunohistochemical staining was performed to compare the extents of neovascularization and CD11b＋ cell infiltration. Real-time polymerase chain reaction was performed to quantify the expression of inflammatory cytokines and VEGF-A in the ***： Trapping VEGF-A with aflibercept resulted in significantly decreased angiogenesis and inflammation compared with the dexamethasone and PBS treatments in the dry eye corneas（all P 〈0.05）, but with no such effects in non-dry eyes. The anti-inflammatory and antiangiogenic effects of VEGF-A trapping were stronger than those of dexamethasone in both dry eye and non-dry eye corneas（all P 〈0.05）. The levels of RNA expression of VEGF-A, TNF-alpha, and IL-6 in the aflibercept subgroup were significantly decreasedcompared with those in the PBS subgroup in the dry eye ***： Compared with non-dry eye corneas,dry eye corneas have greater amounts of inflammation and neovascularization and also have a more robust response to anti-inflammatory and anti-angiogenic agents after ocular surface surgery. Trapping VEGF-A is effective in decreasing both angiogenesis and inflammation in dry eye corneas after ocular surface surgery.

关键词： dry eye aflibercept neovascularization inflammation dexamethasone

Evaluation the Properties of Titanium Matrix Composites by Melting Route Synthesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2008年第1期24卷 105-109页

作者： Bong-Jae choi Si-young Sung Myoung-Gyun Kim young-Jig Kim Department of Advanced Materials Engineering Sungkyunkwan University SuwonKorea Department of Advanced Materials Engineering Sungkyunkwan University SuwonKorea Materials & Processing Engineering CenterR&D DivisionKorea Automotive Technology InstituteChonanChungnamKorea Research Institute of Industrial Science and Technology Pohang Korea

The main purpose of this study is an in-situ synthesis of （TiB＋TiC） hybrid titanium matrix composites （TMCs） by vacuum induction melting method and to verify its mechanical *** melting route was adopted to synthesize the commercial pure titanium （cp Ti） and granular boron carbide （B-4C）.The reinforcements,the fraction of 10 ***,were formed by adding 1.88 wt pct B-4C to cp *** in-situ synthesis of TMCs,electron probe micro-analysis elemental mapping was carried out to confirm the distribution and shape of *** cone-on-disk type sliding wear test was also done for the identification of *** is concluded that （TiB＋TiC） hybrid TMCs can be in-situ synthesized and has better wear properties than H13.

关键词： Titanium matrix composites H13 In-situ Wear