Effect of trapping vascular endothelial growth factor-A in a murine model of dry eye with inflammatory neovascularization
Effect of trapping vascular endothelial growth factor-A in a murine model of dry eye with inflammatory neovascularization作者机构:Department of OphthalmologySt.Vincent's HospitalCollege of Medicinethe Catholic University of Korea Research Institute of Medical ScienceSt.Vincent's Hospitalthe Catholic University of Korea
出 版 物:《International Journal of Ophthalmology(English edition)》 (国际眼科杂志(英文版))
年 卷 期:2016年第9卷第11期
页 面:1541-1548页
核心收录:
学科分类:1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金:Supported by the St.Vincent's Hospital Research Institute of Medical Science Foundation(No.SVHR-2015-13)
主 题:dry eye aflibercept neovascularization inflammation dexamethasone
摘 要:AIM: To evaluate whether trapping vascular endothelial growth factor-A(VEGF-A) would suppress angiogenesis and inflammation in dry eye corneas in a murine corneal suture ***: We established two groups of animals, one with non-dry eyes and the other with induced dry *** both groups, a corneal suture model was used to induce inflammation and neovascularization. Each of two groups was again divided into three subgroups according to the treatment; subgroup I(aflibercept),subgroup II(dexamethasone) and subgroup III(phosphate buffered saline, PBS). Corneas were harvested and immunohistochemical staining was performed to compare the extents of neovascularization and CD11b+ cell infiltration. Real-time polymerase chain reaction was performed to quantify the expression of inflammatory cytokines and VEGF-A in the ***: Trapping VEGF-A with aflibercept resulted in significantly decreased angiogenesis and inflammation compared with the dexamethasone and PBS treatments in the dry eye corneas(all P 〈0.05), but with no such effects in non-dry eyes. The anti-inflammatory and antiangiogenic effects of VEGF-A trapping were stronger than those of dexamethasone in both dry eye and non-dry eye corneas(all P 〈0.05). The levels of RNA expression of VEGF-A, TNF-alpha, and IL-6 in the aflibercept subgroup were significantly decreasedcompared with those in the PBS subgroup in the dry eye ***: Compared with non-dry eye corneas,dry eye corneas have greater amounts of inflammation and neovascularization and also have a more robust response to anti-inflammatory and anti-angiogenic agents after ocular surface surgery. Trapping VEGF-A is effective in decreasing both angiogenesis and inflammation in dry eye corneas after ocular surface surgery.