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Novel insights into host-pathogen interactions of large yellow croakers(Larimichthys crocea)and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens

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Zoological Research 2020年第3期41卷 314-327页

作者： Yi Tang Ge Xin Ling-Min Zhao Li-Xing Huang Ying-Xue Qin yong-quan su Wei-Qiang Zheng Bin Wu Nan Lin Qing-Pi Yan Fisheries College Key Laboratory of Healthy Mariculture for the East China SeaMinistry of AgricultureJimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker Breeding Ningde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extention Center FuzhouFujian 350003China

Host-pathogen interactions are highly complex,involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, timeresolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection(dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study hostpathogen interactions between L. crocea and ***. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor,Toll-like receptor signaling, and other immunerelated pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune *** P. plecoglossicida, the expression levels of motility-related genes and flagellum assemblyrelated genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and ***.

关键词： Larimichthys crocea Pseudomonas plecoglossicida Dual RNA-seq Host-pathogen interactions Dynamic transcriptome

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Dual RNA-seq provides novel insight into the roles of dks A from Pseudomonas plecoglossicida in pathogenhost interactions with large yellow croakers(Larimichthys crocea)

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Zoological Research 2020年第4期41卷 410-422页

作者： Lu-Ying Wang Zi-XuLiu Ling-Min Zhao Li-Xing Huang Ying-Xue Qin yong-quan su Wei-Qiang Zheng Fan Wang Qing-Pi Yan Fisheries College Jimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker Breeding Ningde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extension Center FuzhouFujian 350003China

Pseudomonas plecoglossicida is a rod-shaped,gram-negative bacterium with *** causes visceral white spot disease and high mortality in Larimichthys crocea during culture,resulting in serious economic *** of transcriptome and quantitative real-time polymerase chain reaction(PCR)data showed that dks A gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides(log2 FC=3.12,Psubstantial down-regulation in cellular component-,flagellum-,and ribosome assembly-related genes in ***,and the significant up-regulation of fli C may be a way in which virulence is maintained in *** GO and KEGG results showed that RNAi strain infection in *** led to the down-regulation of inflammatory factor genes in immune-related pathways,which were associated with multiple immune response *** also showed that dks A was a virulence gene in *** with the wild-type strains,RNAi strain infection induced a weaker immune response in ***.

关键词： mortality infection yellow

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轻质点阵夹芯板热屈曲的实验研究

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实验力学 2018年第2期33卷 167-174页

作者：励争周洁李冰刘咏泉苏先樾北京大学工学院力学与工程科学系及湍流与复杂系统国家重点实验室

轻质点阵结构在高温工作环境下易产生失稳破坏，因此，研究其在热载荷条件下的稳定性是极为重要的。本文采用有限元数值计算和实验方法研究了轻质点阵夹芯板在热载荷作用下的稳定性问题。通过与点阵夹芯板的单向压力失稳形式相比较发现... 详细信息

轻质点阵结构在高温工作环境下易产生失稳破坏，因此，研究其在热载荷条件下的稳定性是极为重要的。本文采用有限元数值计算和实验方法研究了轻质点阵夹芯板在热载荷作用下的稳定性问题。通过与点阵夹芯板的单向压力失稳形式相比较发现：点阵夹芯板的单向压力失稳和热栽荷作用失稳的形式并不完全一致；而且，面板厚度及其与夹芯杆件的粘接程度对点阵夹芯板的稳定性有着重要的影响。此外，本文针对将温度变化等效为热应力这一等效模型的适用性进行了讨论，发现等效均匀化理论只适用于面板较厚且产生整体失稳的情况。

关键词：点阵夹芯板热稳定性屈曲模式

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A Facile Synthesis of （S）-（-）-4-tert-Butyldimethylsiloxy-cyclopenten-1-one

A Facile Synthesis of （S）-（-）-4-tert-Butyldimethylsiloxy...

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中国化学会第四届有机化学学术会议

作者： PENG, Xuan-Jia suN, yong-quan su,Ying SHE, Xue-Gong PAN, Xin-Fu (State Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou 730000)

＜正＞（S）-（-）-4-tert-Butyldimethylsiloxy-2-cyclopenten-l-one （（S）-1）, has proven to be valuable precursors for the synthesis of prostaglandins and other important biologically active substances in optically active form. As a result, several synthesis of them has appeared which can be classified in two categories: enzymatic and synthetic. But many of these methods employ numerous steps, expensive reagents, complicated reaction apparatus, and/or cyclopentadiene. During the course of a study directed toward the large-scale preparation of （S）-1 we needed to

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Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

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Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin Zeng Le Wang Wenwu Zhang Gang Li Xing Cheng Peiqiang su yong Wan Xiang Li Department of Spine surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

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Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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Traditional Medicine Research 2025年第2期10卷 16-29页

作者： Xia-Jie quan Hao Liang yong-Hong Tang Li Jiang Xiong-Ying Ji Feng-Ying Zhang Ping Zhang Bo Ouyang Department of Information The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Neurology The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Gastroscopy The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Traditional Chinese Medicine The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China

Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of ***,the specific mechanism of action of SXLJF remains ***:The mouse model of insomnia was established by intraperitoneal injection of ***-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive *** open field test and pentobarbital-induced sleeping test were ***-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating ***,key targets were validated using ***:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep *** and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of ***,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,***-qPCR results supported the role of TH in the mechanism of SXLJF in treating ***:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关键词： Shangxia Liangji formula insomnia metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism

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Transient electro-osmotic and pressure driven flows of two-layer fluids through a slit microchannel

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Acta Mechanica Sinica 2013年第4期29卷 534-542页

作者： Jie su yong-Jun Jian Long Chang quan-Sheng Liu School of Mathematical Science Inner Mongolia University School of Mathematics and Statistics Inner Mongolia University of Finance and Economics

By method of the Laplace transform, this arti- cle presents semi-analytical solutions for transient electro- osmotic and pressure-driven flows （EOF/PDF） of two-layer fluids between microparallel plates. The linearized Poisson- Boltzmann equation and the Cauchy momentum equation have been solved in this article. At the interface, the Maxwell stress is included as the boundary condition. By numerical computations of the inverse Laplace transform, the effects of dielectric constant ratio e, density ratio p, pressure ratio p, viscosity ratioμ of layer II to layer I, interface zeta potential difference △ψ, interface charge density jump Q, the ratios of maximum electro-osmotic velocity to pressure velocity , and the normalized pressure gradient B on transient veloc- ity amplitude are *** find the velocity amplitude becomes large with the interface zeta potential difference and becomes small with the increase of the viscosity. The ve- locity will be large with the increases of dielectric constant ratio; the density ratio almost does not influence the EOF ve- locity. Larger interface charge density jump leads to a strong jump of velocity at the interface. Additionally, the effects of the thickness of fluid layers （hi and h2） and pressure gradient on the velocity are also investigated.

关键词： Hydromechanics ~ Micro-parallel plates ~ Elec-tric double layer (EDL) ~ Unsteady EOF/PDF ~ Two-layerNewtonian fluids

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耐As_2O_3的白血病细胞系SHI-1/AS2的建立及耐药机制的初步研究

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苏州大学学报（医学版） 2009年第4期29卷 597-600,603,F0002页

作者：王勇薛永权陈苏宁吴亚芳潘金兰张俊沈娟苏州大学附属第一医院江苏省血液研究所卫生部血栓与止血重点实验室江苏苏州215006

目的建立三氧化二砷(As2O3)的耐药细胞株,进一步从分子水平研究其耐药机制。方法采用高度短时间接触培养方法建立耐As2O3的白血病细胞系(SHI-1/AS2)。细胞倍增时间和耐药倍数测定采用MTT法;细胞周期和细胞免疫表型测定采用流式细胞分析... 详细信息

目的建立三氧化二砷(As2O3)的耐药细胞株,进一步从分子水平研究其耐药机制。方法采用高度短时间接触培养方法建立耐As2O3的白血病细胞系(SHI-1/AS2)。细胞倍增时间和耐药倍数测定采用MTT法;细胞周期和细胞免疫表型测定采用流式细胞分析术;常见的10个耐药基因检测和比较采用实时定量PCR(RQ-PCR)。结果耐As2O3的白血病细胞系SHI-1/AS2的细胞倍增时间与SHI-1相似,差异无统计学意义(P>0.05)。SHI-1/AS2细胞G0/G1期细胞少于SHI-1细胞,而G2/M、S期细胞则多于SHI-1细胞,差异均有高度统计学意义(均P<0.01)。SHI-1/AS2细胞对As2O3的耐药倍数是SHI-1的2.7倍,差异有统计学意义(P<0.01)。SHI-1/AS2细胞的集落形成率高于SHI-1细胞。SHI-1/AS2细胞的耐药相关基因Bcl-2有明显上调,Bax、Fas及MGMT下调,而其他耐药相关基因无明显变化。结论建立一株对As2O3产生耐药的白血病细胞系SHI-1/AS2,耐药倍数是SHI-1的2.7倍,其耐药机制与Bcl-2、Bax和Fas基因介导的细胞凋亡调控有关。

关键词：抗药性砷剂白血病

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Mathematical simulation of direct reduction process in zinc-bearing pellets

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International Journal of Minerals,Metallurgy and Materials 2013年第11期20卷 1042-1049页

作者： Ying Liu Fu-yong su Zhi Wen Zhi Li Hai-quan yong Xiao-hong Feng School of Mechanical Engineering University of Science and Technology Beijing CISDI Industrial Furnace Co.Ltd.

A one-dimensional unsteady mathematical model was established to describe direct reduction in a composite pellet made of metallurgical dust. The model considered heat transfer, mass transfer, and chemical reactions including iron oxide reductions, zinc oxide reduction and carbon gasification, and it was numerically solved by the tridiagonal matrix algorithm （TDMA）. In order to verify the model, an experiment was performed, in which the profiles of temperature and zinc removal rate were measured during the reduction process. Results calculated by the mathematical model were in fairly good agreement with experimental data. Finally, the effects of furnace temperature, pellet size, and carbon content were investigated by model calculations. It is found that the pellet temperature curve can be divided into four parts according to heating rate. Also, the zinc removal rate increases with the increase of furnace temperature and the decrease of pellet size, and carbon content in the pellet has little influence on the zinc removal rate.

关键词： metallurgical dust ore pellets direct reduction process mathematical models

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A Note on Integral Structures in Some Locally Algebraic Representations of GL_(2)

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Acta Mathematica Sinica,English Series 2021年第1期37卷 59-72页

作者： yong quan HU Morningside Center of Mathematics Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190P.R.China Chinese Academy of Sciences University of Chinese Academy of SciencesBeijing 100049P.R.China

In the p-adic local Langlands correspondence for GL2(Qp),the following theorem of Berger and Breuil has played an important role:the locally algebraic representations of GL2(Qp)associated to crystabelline Galois representations admit a unique unitary *** this note,we give a new proof of the weaker statement that the locally algebraic representations admit at most one unitary completion and such a completion is automatically *** proof is purely representation theoretic,involving neither(ψ,Γ)-module techniques nor global *** F is a finite extension of Qp,we also get a simpler proof of a theorem of Vignéras for the existence of integral structures for(locally algebraic)special series and for(smooth)tamely ramified principal series.

关键词： Invariant norm diagram

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