Novel insights into host-pathogen interactions of large yellow croakers(Larimichthys crocea)and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens

作     者：Yi Tang Ge Xin Ling-Min Zhao Li-Xing Huang Ying-Xue Qin Yong-Quan Su Wei-Qiang Zheng Bin Wu Nan Lin Qing-Pi Yan Yi Tang;Ge Xin;Ling-Min Zhao;Li-Xing Huang;Ying-Xue Qin;Yong-Quan Su;Wei-Qiang Zheng;Bin Wu;Nan Lin;Qing-Pi Yan

作者机构：Fisheries CollegeKey Laboratory of Healthy Mariculture for the East China SeaMinistry of AgricultureJimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker BreedingNingde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extention CenterFuzhouFujian 350003China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2020年第41卷第3期

页      面：314-327页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：the National Natural Science Foundation of China(31672694,31972836) Fujian Provincial Special Fund for Marine and Fishery Protection and Development(MCZ062) 

主　　题：Larimichthys crocea Pseudomonas plecoglossicida Dual RNA-seq Host-pathogen interactions Dynamic transcriptome 

摘      要：Host-pathogen interactions are highly complex,involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, timeresolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection(dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study hostpathogen interactions between L. crocea and ***. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor,Toll-like receptor signaling, and other immunerelated pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune *** P. plecoglossicida, the expression levels of motility-related genes and flagellum assemblyrelated genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and ***.