Dual RNA-seq provides novel insight into the roles of dks A from Pseudomonas plecoglossicida in pathogenhost interactions with large yellow croakers(Larimichthys crocea)

作     者：Lu-Ying Wang Zi-XuLiu Ling-Min Zhao Li-Xing Huang Ying-Xue Qin Yong-Quan Su Wei-Qiang Zheng Fan Wang Qing-Pi Yan Lu-Ying Wang;Zi-Xu Liu;Ling-Min Zhao;Li-Xing Huang;Ying-Xue Qin;Yong-Quan Su;Wei-Qiang Zheng;Fan Wang;Qing-Pi Yan

作者机构：Fisheries CollegeJimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker BreedingNingde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extension CenterFuzhouFujian 350003China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2020年第41卷第4期

页      面：410-422页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：This work was supported by the National Natural Science Foundation of China(31672694) Fujian Provincial Special Fund for Marine and Fishery Protection and Development(MCZ062) and Open Fund of Fujian Province Key Laboratory of Special Aquatic Formula Feed(2019-01) 

主　　题：mortality infection yellow 

摘      要：Pseudomonas plecoglossicida is a rod-shaped,gram-negative bacterium with *** causes visceral white spot disease and high mortality in Larimichthys crocea during culture,resulting in serious economic *** of transcriptome and quantitative real-time polymerase chain reaction(PCR)data showed that dks A gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides(log2 FC=3.12,P0.001).RNAi of five sh RNAs significantly reduced the expression of dks A in ***,and the optimal silencing efficiency was 96.23%.Compared with wild-type strains,the symptoms of visceral white spot disease in *** infected with RNAi strains were reduced,with time of death delayed by 48 h and mortality reduced by 25%.The dks A silencing led to a substantial down-regulation in cellular component-,flagellum-,and ribosome assembly-related genes in ***,and the significant up-regulation of fli C may be a way in which virulence is maintained in *** GO and KEGG results showed that RNAi strain infection in *** led to the down-regulation of inflammatory factor genes in immune-related pathways,which were associated with multiple immune response *** also showed that dks A was a virulence gene in *** with the wild-type strains,RNAi strain infection induced a weaker immune response in ***.