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OsWRKY78 regulates panicle exsertion via gibberellin signaling pathway in rice

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Journal of Integrative Plant Biology 2024年第4期66卷 771-786页

作者： Enyang Mei Mingliang He Min Xu Jiaqi Tang Jiali liu yingxiang liu Zhipeng Hong Xiufeng Li Zhenyu Wang Qingjie Guan Xiaojie Tian Qingyun Bu State Key Laboratory of Black Soils Conservation and Utilization Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Saline-alkali Vegetation Ecology Restoration Northeast Forestry UniversityMinistry of EducationHarbin 150040China College of Life Science Northeast Forestry UniversityHarbin 150040China

Panicle exsertion is one of the crucial agronomic traits in rice(Oryza sativa).Shortening of panicle exsertion often leads to panicle enclosure and severely reduces seed ***(GA)plays important roles in regulating panicle ***,the underlying mechanism and the relative regulatory network remain ***,we characterized the oswrky78 mutant showing severe panicle enclosure,and found that the defect of oswrky78 is caused by decreased bioactive GA *** analysis demonstrates that OsWRKY78 can directly activate GA biosynthesis and indirectly suppress GA ***,we found OsWRKY78 can interact with and be phosphorylated by mitogen-activated protein kinase(MAPK)kinase OsMAPK6,and this phosphorylation can enhance OsWRKY78 stability and is necessary for its biological *** together,these results not only reveal the critical function of OsWRKY78,but also reveal its mechanism via mediating crosstalk between MAPK and the GA signaling pathway in regulating panicle exsertion.

关键词： gibberellin MAPK cascades OsWRKY78 panicle exsertion rice

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小片蝽线粒体基因组及蝽科系统发育分析

山西农业科学 2024年第2期52卷 78-88页

作者：刘迎香赵清山西农业大学植物保护学院山西太谷030801

为了从线粒体基因组水平探讨小片蝽Sciocoris lateralis在蝽科的分类地位,丰富蝽科线粒体基因组基本数据,为蝽科系统发育进化研究提供一定的理论依据,通过高通量测序,首次测定并分析了小片蝽完整线粒体基因组(Genbank登录号:OP531920),... 详细信息

为了从线粒体基因组水平探讨小片蝽Sciocoris lateralis在蝽科的分类地位,丰富蝽科线粒体基因组基本数据,为蝽科系统发育进化研究提供一定的理论依据,通过高通量测序,首次测定并分析了小片蝽完整线粒体基因组(Genbank登录号:OP531920),提取蝽科物种的13个蛋白编码串联序列(PCGs),使用最大似然法、贝叶斯法构建系统发育树。结果显示,小片蝽昆虫的线粒体全基因组长度为15 445 bp,包括13个蛋白编码基因(Protein-codinggenes,PCGs)、2个rRNA基因(ribosomeRNAs,rRNAs)、22个tRNA基因(transferRNAs,tRNAs)和1个控制区(Control region)。小片蝽线粒体基因组结构排序与其他蝽科物种一致,均无基因重排现象。小片蝽线粒体全序列AT含量为74.26%,GC含量为25.74%。13个蛋白编码基因中,cox1、nad1和nad6的起始密码子为TTG,其余10个蛋白编码基因的起始密码子是ATT、ATA、ATG。在所测得的tRNA基因中,trnS1和trnV缺失DHU臂,其他20个tRNA均能折叠形成典型的三叶草结构。基因错配方式主要为G-U错配。基于13个PCGs构建的蝽科系统发育树结果显示,小片蝽与斑须蝽的亲缘关系最近并且位于蝽亚科分支,支持二星蝽族Eysarcorini和Strachiini族的单系性,而棕蝽族Caystrini和腹沟族Halyini互为姐妹群,这与传统形态学分类结果一致;益蝽亚科与蝽亚科曼蝽属(Menida)形成姐妹群关系,该系统发育关系与传统形态分类学研究结果不一致。

关键词：小片蝽线粒体基因组蝽科系统发育

Piezoelectric inertial robot for operating in small pipelines based on stick-slip mechanism:modeling and experiment

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Frontiers of Mechanical Engineering 2022年第3期17卷 109-124页

作者： Jichun XING Chao NING yingxiang liu Ian HOWARD School of Mechanical Engineering Yanshan UniversityQinhuangdao066004China State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin150001China School of Civil and Mechanical Engineering Curtin UniversityPerth6001Australia

Small pipes exist in industrial and biomedical fields,and require microrobots with high operational precision and large load capacity to inspect or perform functional tasks.A piezoelectric inertial pipeline robot using a“stick-slip”mechanism was proposed to address this *** this study,the driving principle of the proposed robot was analyzed,and the strategy of the design scheme was presented.A dynamics model of the stick-slip system was established by combining the dynamics model of the driving foot system and the LuGre friction model,and the simulation analysis of the effect of system parameters on the operating trajectory was *** experimental system was established to examine the output characteristics of the proposed *** results show that the proposed pipeline robot with inertial stick-slip mechanism has a great load capacity of carrying 4.6 times(70 g)its own mass and high positioning *** speed of the pipeline robot can reach up to 3.5 mm/s(3 mm/s)in the forward(backward)direction,with a minimum step distance of 4µ*** potential application for fine operation in the pipe is exhibited by a demonstration of contactless transport.

关键词： pipeline robot piezoelectric inertial drive stick-slip large load capacity dynamics model small pipeline

A 3-DOF piezoelectric robot with continuous walking gait aiming at cross-scale smooth motion

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Science China(Technological Sciences) 2023年第1期66卷 233-242页

作者： YU HongPeng liu yingxiang DENG Jie ZHANG ShiJing CHEN WeiShan State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 15000lChina

Piezoelectric robots play important roles in the field of micromanipulation, but it is difficult for them to generate steady precision motion at any moment. In order to eliminate the changing inertial force and improve the motion smoothness, this work proposes a piezoelectric robot with continuous walking gait inspired by ants. The idea is verified with theoretical models and numerical simulation, and the performances are evaluated with experiments. The robot is proven to have the ability to generate 3-DOF(dgeree of freedom) continuous smooth motions with constant speeds. The maximum and minimum smooth velocities have a difference of six orders of magnitude, realizing cross-scale velocity control. Besides, the motion resolution reaches several nanometers with the unlimited workspace, so the cross-scale displacement control can be also obtained. Furthermore, with great robustness against varying loads, the stable actuation capability of the robot is more than 22 times of the self-weight. To sum up,the proposed robot generates cross-scale smooth motion in both aspects of displacement and velocity, so it has good prospects in the applications requiring steady precision motion. The design philosophy and research methods in this work can be valuable references for further advances of micromanipulation robots.

关键词： piezoelectric robot continuous walking gait cross-scale displacement cross-scale velocity smooth motion constant speed

Design and experiments of a small resonant inchworm piezoelectric robot

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Science China(Technological Sciences) 2023年第3期66卷 821-829页

作者： DENG Jie YANG ChengLin liu yingxiang ZHANG ShiJing LI Jing MA XueFeng XIE Hui State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China

A small resonant inchworm piezoelectric robot with six driving feet which are set evenly along the circumference is proposed and tested.A bonded-type structure is adopted to realize a small *** radial bending vibration mode and longitudinal vibration mode are excited at the same *** superposition of these two vibration modes makes the driving feet produce elliptical *** the driving force can be generated by friction coupling between the driving foot and the operating *** structure of the robot is designed by finite element *** geometric parameters are adjusted to make the resonant frequencies of the vibration modes as close as *** elliptical trajectories generated at the driving feet are discussed in *** vibration and motion characteristics of the prototype are tested,and the resonant frequencies of the radial bending mode and the longitudinal vibration mode are degenerated *** optimal working frequency of the prototype is 21.5 *** maximum speed of the prototype is 200 mm/s,and the displacement resolution is 0.71μ*** measured results show that the resonant inchworm piezoelectric robot can be used for fast and high-precision transportation in narrow space.

关键词： piezoelectric robot resonant actuation inchworm movement bending and longitudinal vibration

Design of a linear-rotary ultrasonic motor for optical focusing inspired by the bionic motion principles of the earthworms

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International Journal of Smart and Nano Materials 2022年第2期13卷 346-365页

作者： Yingzhi Wang Jie Deng Shijing Zhang He Li Weishan Chen yingxiang liu State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbinHeilongjiang ProvinceChina

Multi-motor configuration of multi-DOF(degree of freedom)optical system is a major source of redundant structure,putting a limitation on the simple and miniaturized ***,a novel two-DOF ultrasonic motor(USM)is proposed to provide a feasible method of application in the lens autofocus of the optical *** proposed USM operates by one longitudinal mode and two orthogonal bending modes,which is inspired by the bionic motion principle of the *** frequency degeneration among the three working modes is per-formed,and the working principle of the USM is verified via the FEM simulation.A prototype of the two-DOF USM is fabricated,and its mechanical output characteristics are *** experimental results indicate that the prototype achieves the maximum rotary and linear speeds of 3319.6 rpm and 57.6 mm/s,***,we demonstrate the result of a simple focusing experiment using the prototype and obtain a series of clear pictures,which verifies the feasibility of application in the optical focusing system.

关键词： Ultrasonic motor bondedtype two-DOF motions optical focusing bionic motion

Modeling and experiments of a nano-positioning and high frequency scanning piezoelectric platform based on function module actuator

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Science China(Technological Sciences) 2020年第12期63卷 2541-2552页

作者： DENG Jie liu yingxiang ZHANG ShiJing liu JunKao School of Mechatronics and Engineering Harbin Institute of TechnologyHarbin 150001China

A piezoelectric platform using function module actuator is presented to achieve nano-positioning and high frequency scanning in large working range. A function module actuator is designed to produce a pair of orthogonal bending deformations and a longitudinal deformation through partition exciting. The bending deformations are used to actuate the planar motion,while the longitudinal deformation is utilized to dynamically adjust the driving force and broaden the scanning frequency. The dynamic model of the platform system is developed. The open-loop performances of a prototype are first tested: a scan frequency of 308 Hz in a scanning range of 3.368 μm×3.396 μm is measured in direct actuation mode,and the displacement resolution is measured to be 16 nm;maximum speed is measured to be 3.38 mm s^-1 in the inertial actuation mode. Furthermore,the closedloop experiments are carried out and a switching strategy is proposed to obtain the switching of the inertial and direct actuation modes automatically;the platform achieves the scanning with frequency of 300 Hz at the set position.

关键词： piezoelectric actuator function modules nano-positioning platform large working range high frequency scanning

A novel sensitive piezoelectric mass balance used for weightless environment

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Science China(Technological Sciences) 2021年第4期64卷 745-754页

作者： ZHANG ShiJing liu yingxiang DENG Jie liu JunKao School of Mechatronics and Engineering Harbin Institute of TechnologyHarbin 150001China

A novel sensitive piezoelectric mass balance(PEMB)was developed for using in weightless *** mass of the measured object was obtained by detecting the resonant frequency shift of the *** weighing method was illustrated *** order to build the relationship between the resonant frequency shift and the measured mass,the equivalent stiffness coefficient and the equivalent concentrated mass of the PEMB were analyzed *** influence of the structural and material parameters on the sensitivity was analyzed,which provided the design reference to improve the *** finite-element-method(FEM)static analysis of the PEMB was carried out with ANSYS software to investigate its shock ***,the vibration test and calibration experiment effectively verified the theoretical analyses *** developed PEMB holds merits of independence on acceleration field,independence on properties of the measured object and with shock ***,it can be a suitable candidate for measuring mass of solid samples with unknown properties in the weightless environment.

关键词： mass measurement piezoelectric mass balance resonant frequency shift weightless environment

bZIP71 delays flowering by suppressing Ehd1 expression in rice

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Journal of Integrative Plant Biology 2022年第7期64卷 1352-1363页

作者： Xiufeng Li Xiaojie Tian Mingliang He Xinxin liu Zhiyong Li Jiaqi Tang Enyang Mei Min Xu yingxiang liu Zhenyu Wang Qingjie Guan Wei Meng Jun Fang Jian Zhang Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breedingthe Chinese Academy ofSciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 311400China The Innovative Academy of Seed Design the Chinese Academy of SciencesBeijing 100101China

Flowering time is a fundamental factor determining the global distribution and final yield of rice(Oryza sativa).Although diverse flowering time genes have been reported in this crop,the transcriptional regulation of its key flowering genes are poorly ***,we report that a basic leucine zipper transcription factor,bZIP71,functions as a flowering *** overexpression of bZIP71 delays flowering,while the bzip71 mutant flowers early in both long-day and short-day conditions.A genetic analysis showed that the regulation of flowering by bZIP71 might be independent of Heading date 2(Hd2),Hd4,and ***,bZIP71 directly associates with the Early heading date 1(Ehd1)promoter and represses its transcription,and genetically the function of bZIP71 is impaired in the ehd1 ***,bZIP71 interacts with major components of polycomb repressive complex 2(PRC2),SET domain group protein 711(SDG711),and Fertilization independent endosperm 2(FIE2),through which bZIP71 regulates the H3K27me3 level of *** together,we present a transcriptional regulatory mechanism in which bZIP71 enhances the H3K27me3 level of Ehd1 and transcriptionally represses its expression,which not only offers a novel insight into a flowering pathway,but also provides a valuable putative target for the genetic engineering and breeding of elite rice cultivars.

关键词： flowering time H3K27me3 rice transcriptional regulation