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Glycerophospholipids pathways and chromosomal instability in gastric cancer: Global lipidomics analysis

World Journal of Gastrointestinal Oncology 2019年第3期11卷 181-194页

作者： Cheng-Yu Hung Ta-Sen Yeh Cheng-Kun Tsai Ren-Chin Wu ying-Chieh lai Meng-Han Chiang Kuan-ying Lu Chia-Ni Lin Mei-Ling Cheng Gigin Lin Molecular Medicine Research Center Chang Gung University Clinical Metabolomics Core Lab Chang Gung Memorial Hospital at Linkou and Chang Gung University Department of Medical Imaging and Intervention Imaging Core Lab Institute for Radiological Research Chang Gung Memorial Hospital at Linkou and Chang Gung University Department of Surgery Chang Gung Memorial Hospital at Linkou and Chang Gung University Department of Pathology Chang Gung Memorial Hospital at Linkou and Chang Gung University Department of Laboratory Medicine Chang Gung Memorial Hospital at Linkou and Chang Gung University Department of Biomedical Science College of Medicine Chang Gung University

BACKGROUND Based on the breakthrough of genomics analysis, The Cancer Genome Atlas Research Group recently proposed an integrative genomic analysis, dividing gastric cancer(GC) into four subtypes, characterized by the chromosomal instability(CIN) status. However, the CIN status of GC is still vaguely characterized and lacking the valuable easy-to-use CIN markers to diagnosis in molecular and histological *** To explore the associations of CIN with downstream lipidomics *** We collected cancerous and noncancerous tissue samples from 18 patients with GC; the samples were divided into CIN and non-CIN types based on the system of The Cancer Genome Atlas Research Group and 409 sequenced oncogenes and tumor suppressor genes. We identified the lipidomics profiles of the GC samples and samples of their adjacent noncancerous tissues by using liquid chromatography–mass spectrometry. Furthermore, we selected leading metabolites based on variable importance in projection scores of > 1.0 and P <*** Twelve men and six women participated in this study; the participants had a median age of 67.5 years(range, 52–87 years) and were divided into CIN(n = 9)and non-CIN(n = 9) groups. The GC samples exhibited distinct profiles of lysophosphocholine, phosphocholine, phosphatidylethanolamine,phosphatidylinositol, phosphoserine, sphingomyelin, ceramide, and triglycerides compared with their adjacent noncancerous tissues. The glycerophospholipid levels(phosphocholine, phosphatidylethanolamine, and phosphatidylinositol)were 1.4-to 2.3-times higher in the CIN group compared with the non-CIN group(P < 0.05). Alterations in the glycerolipid and glycerophospholipid pathways indicated progression of GC toward *** The lipidomics profiles of GC samples were distinct from those of their adjacent noncancerous tissues. CIN status of GC is primarily associated with downstream lipidomics in the glycerophospholipid pathway.

关键词： Chromosomal instability Gastric cancer Glycerophospholipids Metabolomics Lipidomics profile

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Metabolomic alterations and chromosomal instability status in gastric cancer

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World Journal of Gastroenterology 2018年第33期24卷 3760-3769页

作者： Cheng-Kun Tsai Ta-Sen Yeh Ren-Chin Wu ying-Chieh lai Meng-Han Chiang Kuan-ying Lu Cheng-Yu Hung Hung-Yao Ho Mei-Ling Cheng Gigin Lin Clinical Metabolomics Core Lab Chang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Department of Medical Imaging and Intervention Imaging Core LabInstitute for Radiological ResearchChang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Chang Gung Memorial Hospital at Linkou and Chang Gung University Taoyuan 333Taiwan Department of Pathology Chang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Department of Medical Biotechnology and Laboratory Science College of MedicineChang Gung UniversityTaoyuan 333Taiwan Department of Biomedical Science College of MedicineChang Gung UniversityTaoyuan 333Taiwan

AIM To explore the correlation of metabolomics profiles ofgastric cancer(GC) with its chromosomal instability(CIN) *** Nineteen GC patients were classified as CIN and nonCIN type by The Cancer Genome Atlas Research Group system, based on 409 oncogenes and tumor suppressor genes sequenced. The aqueous metabolites of the GC tumor and its surrounding adjacent healthy tissues were identified through liquid chromatographymass spectrometry. Groups were compared by defining variable importance in projection score of > 1.2, a fold change value or its reciprocal of > 1.2, and a P value of < 0.05 as a significant *** In total,twelve men and seven women were enrolled, with a median age of 66 years(range, 47-87 years). The numbers of gene alterations in the CIN GC group were significantly higher than those in the non-CIN GC(32-218 vs 2-17; P < 0.0005). Compared with the adjacent healthy tissues, GC tumors demonstrated significantly higher aspartic acid, citicoline, glutamic acid, oxidized glutathione, succinyladenosine, and uridine diphosphate-Nacetylglucosamine levels, but significantly lower butyrylcarnitine, glutathione hydroxyhexanoycarnitine, inosinic acid, isovalerylcarnitine, and threonine levels(all P < 0.05). CIN tumors contained significantly higher phosphocholine and uridine 5'-monophosphate levels but significantly lower beta-citryl-L-glutamic acid levels than did non-CIN tumors(all P < 0.05). CIN GC tumors demonstrated additional altered pathways involving alanine, aspartate, and glutamate metabolism, glyoxylate and dicarboxylate metabolism, histidine metabolism, and phenylalanine, tyrosine, and tryptophan *** Metabolomic profiles of GC tumors and the adjacent healthy tissue are distinct, and the CIN status is associated with downstream metabolic alterations in GC.

关键词： Gastric cancer Metabolomics Oncogene Copy-number Chromosomal instability Liquid chromatography-mass spectrometry

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Resection with limb salvage in an Asian male adolescent withEwing’s sarcoma: A case report

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World Journal of Clinical Cases 2022年第22期10卷 7960-7967页

作者： Chien-ying lai Kuan-Ju Chen Tsung-Yu Ho Ling-Yi Li Chien-Chung Kuo Hsien-Te Chen Yi-Chin Fong Department of Orthopedic Surgery China Medical University HospitalTaichung 404Taiwan Spine Center China Medical University HospitalTaichung 404Taiwan School of Medicine China Medical UniversityTaichung 404Taiwan

BACKGROUNDEwing’s sarcoma is a highly malignant primary bone tumor that commonly affectschildren. For young patients, multidisciplinary treatment and limb salvage arerecommended, and surgical plans considering the growth potential and boneactivity after tumor resection are *** SUMMARYAn 11-year-old Asian boy had a 1-mo history of a right-sided limping *** revealed a proximal tumor with bone destruction and physealinvolvement over the right femoral neck. He was diagnosed with stage IV(T1N0M1aG3) Ewing’s sarcoma with bilateral lung metastases. Neoadjuvantchemotherapy decreased the tumor size and confined it to the metaphysealregion. The patient underwent four stages of surgery: wide tumor excision plusreconstruction with vascular fibular bone graft plus internal fixation;repeat openreduction and internal fixation;femoral lengthening with orthosis after physealmaturity;and orthosis removal and bone elongation (approximately 6 cm).Following surgery, he could walk without discomfort and had almost equal-sizedbilateral femoral heads, indicating physis preservation. The surgery wassuccessful, and normal femoral head growth was achieved after completeremission. The patient was able to resume normal activities with equal length ofthe bilateral lower *** treatment and reconstruction following resection are important in skeletally immature patients with Ewing’s sarcoma to improve quality of life.

关键词： Ewing’s sarcoma Lower limb discrepancy Orthosis Vascular fibular graft Reconstruction Case report

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Synergistic regulation of fusion pore opening and dilation by SNARE and synaptotagmin-1

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Journal of Molecular Cell Biology 2024年第4期16卷 17-30页

作者： Kaiju Li Kaiyu Li Jiaqi Fan Xing Zhang Chengyan Tao Yijuan Xiang Lele Cui Hao Li Minghan Li Yanjing Zhang Jia Geng ying lai National Clinical Research Center for Geriatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Department of Laboratory Medicine State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation CenterChengdu 610041China Tianfu Jincheng Laboratory City of Future MedicineChengdu 641400China

Fusion pore opening is a transient intermediate state of synaptic vesicle exocytosis,which is highly dynamic and precisely regulated by the soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex and synaptotagmin-1(Syt1).Yet,the regulatory mechanism is not fully *** this work,using single-channel membrane fusion electrophysiology,we determined that SNAREpins are important for driving fusion pore opening and dilation but incapable of regulating the *** Syt1 was added,the closing frequency of fusion pores significantly increased,while the radius of fusion pores mildly *** response to Ca^(2+),SNARE/Syt1 greatly increased the radius of fusion pores and reduced their closing ***,the residue F349 in the C2B domain of Syt1,which mediates Syt1 oligomerization,was required for clamping fusion pore opening in the absence of Ca^(2+),probably by extending the distance between the two ***,in Ca^(2+)-triggered fusion,the primary interface between SNARE and Syt1 plays a critical role in stabilizing and dilating the fusion pore,while the polybasic region of Syt1 C2B domain has a mild effect on increasing the radius of the fusion *** summary,our results suggest that Syt1,SNARE,and the anionic membrane synergically orchestrate the dynamics of fusion pore opening in synaptic vesicle exocytosis.

关键词： SNARE synaptotagmin-1 fusion pore vesicle exocytosis

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Etiological features of cirrhosis inpatients in Beijing, China

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Chinese Medical Journal 2013年第13期126卷 2430-2434页

作者： SONG Guang-jun FENG Bo RAO Hui-ying WEI lai Peking University People's Hospital Peking University Hepatology Institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University Beijing 100044 China

Background The etiological spectrum of cirrhosis has changed over the years, but our knowledge of it is limited. The present study aimed to investigate the etiological features of cirrhosis inpatients and their variation in the past 18 years in Beijing. Methods A retrospective analysis was performed on all patients with cirrhosis diagnosed for the first time in Peking University People＇s Hospital from January 1, 1993, to October 25, 2010. Data were analyzed using SPSS 20.0. Results A total of 2119 cirrhosis inpatients were included in this study： 1412 （66.6%） male and 707 （33.4%） female. Chronic hepatitis B accounted for 58.7%; chronic hepatitis C for 7.6%; chronic hepatitis B and hepatitis C virus co-infection for 0.8% （16 cases）; alcoholic liver disease for 9.4% （200 cases）; and autoimmune diseases for 9.4% （199 cases）. In the past 18 years, the percentage of chronic hepatitis B has decreased from 75.2% to 48.7%; alcoholic liver disease has increased from 5.1% to 10.6%; and autoimmune disease has increased from 2.2% to 12.9%. The percentages of chronic hepatitis B and alcoholic liver disease were higher among men, whereas the percentages of chronic hepatitis C, autoimmune diseases and cryptogenic cirrhosis were higher among women. Conclusions Chronic hepatitis B was still the most common etiology of cirrhosis in China, but the percentage has been decreasing. The percentages of alcoholic liver disease and autoimmune diseases have been increasing. The etiological spectrum of cirrhosis inpatients differed significantly according to sex.

关键词： etiology, cirrhosis, hepatitis B hepatitis C autoimmune liver disease, alcoholic liver disease

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All-China Women's Federation Works to Eliminate Women's Poverty

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Women of China 1996年第3期 8-9页

作者： BAO LI lai ying SUJIE MAN

IN April of 1994, the All-China Women’s Federation (ACWF) sent 15 cadres to poor areas in Guizhou, Yunnan and Gansu provinces, and Guangxi Zhuang and Ningxia Hui Autonomous Regions to make on-the-spot investigations. They visited 105 rural households in 30 villages of 11 counties. They found that over 80 percent of the rural households in

关键词： All-China Women’s Federation Works to Eliminate Women’s Poverty

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Characteristics of Microencapsulated Nutritional Oil for Infant Formula Food

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Journal of Northeast Agricultural University(English Edition) 2009年第3期16卷 41-45页

作者： HAN Lulu lai ying LI Xiaodong Key Laboratory of Dairy Science Ministry of Education Food College Northeast Agricultural University Harbin 150030 China Daoli District Office for Health Inspection and Supervision Harbin 150010 China

Nutritional oil for infant formula food was microencapsulated by the spray drying method with coating materials including maltodextrin （MD）, soy protein isolate （SPI）, and emulsifier （soy lecithin）. Vegetable oil blend was prepared by mixing coconut oil, safflower oil and soybean oil at a ratio to achieve a fatty acid profile comparable to human milk fat （HMF）. The fatty acid composition of the product was determined by capillary gas chromatograph. As a result, the composition was as close as possible to that of HMF, it could be used for infant fomular food to make up some deficiencies of milk powder in nutrition and functional properties. Furthermore, the glass transition temperature （Tg） of the wall material was determined by DSC and its Tg was 66.42℃. It provided a theoretical basis for the storage of the product at the normal temperature.

关键词： microencapsulation nutritional oil characteristic human milk fat infant formula food

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气相色谱质谱法同时测定建筑用胶中苯系物和邻苯二甲酸酯类增塑剂

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分析试验室 2010年第3期29卷 58-62页

作者：赖莺厦门出入境检验检疫局厦门361012

建立了建筑用胶中苯系物和邻苯二甲酸酯类增塑剂等10种物质同时测定的气相色谱质谱法,对提取方法及色谱条件进行了研究,各物质的检出限在0.2～0.8 mg/L之间。在两个添加水平下,水基型和溶剂型建筑用胶中苯系物及邻苯二甲酸酯类增塑剂的... 详细信息

建立了建筑用胶中苯系物和邻苯二甲酸酯类增塑剂等10种物质同时测定的气相色谱质谱法,对提取方法及色谱条件进行了研究,各物质的检出限在0.2～0.8 mg/L之间。在两个添加水平下,水基型和溶剂型建筑用胶中苯系物及邻苯二甲酸酯类增塑剂的回收率在98.1%～101.5%之间。5个实验室间对相同建筑用胶样品测定的相对标准偏差在1.9%～3.9%之间。

关键词：气相色谱质谱法超声波萃取苯系物邻苯二甲酸酯建筑用胶

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一种网络间可信连接协议

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软件学报 2019年第12期30卷 3730-3749页

作者：赖英旭刘岩刘静北京工业大学信息学部计算机学院

为了解决深化“互联网+先进制造业”进程中网络可信互连问题,引入了可信连接架构(trusted connect architecture,简称TCA)技术.基于TCA技术思想,针对网络间可信认证需求,设计了一种支持网络间互连的可信连接协议(TCA-SNI).引入了网络间... 详细信息

为了解决深化“互联网+先进制造业”进程中网络可信互连问题,引入了可信连接架构(trusted connect architecture,简称TCA)技术.基于TCA技术思想,针对网络间可信认证需求,设计了一种支持网络间互连的可信连接协议(TCA-SNI).引入了网络间双向认证过程,给出了TCA-SNI协议的交互过程;使用扩展的SVO逻辑系统对协议进行逻辑推理,证明该协议是安全可靠的;使用Dolev-Yao攻击者模型对协议进行攻击测试,实验结果表明,协议的安全目标均已达成,证明该协议可以抵御真实网络中的攻击.

关键词：可信连接架构可信计算可信连接协议 SVO AVISPA

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Nitrogen application regulates antioxidant capacity and flavonoid metabolism,especially quercetin,in grape seedlings under salt stress

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Journal of Integrative Agriculture 2024年第12期23卷 4074-4092页

作者： Congcong Zhang Han Wang Guojie Nai Lei Ma Xu Lu Haokai Yan Meishuang Gong YuanyuanLi ying lai Zhihui Pu Li Wei Guiping Chen Ping Sun Baihong Chen Shaoying Ma Sheng Li College of Horticulture Gansu Agricultural UniversityLanzhou 730070China College of Life Science and Technology Gansu Agricultural UniversityLanzhou 730070China College of Agronomy Gansu Agricultural UniversityLanzhou 730070China Laboratory and Practice Base Management Center Gansu Agricultural UniversityLanzhou 730070China State Key Laboratory of Aridland Crop Science Gansu Agricultural UniversityLanzhou 730070China

Salt stress is a typical abiotic stress in plants that causes slow growth,stunting,and reduced yield and fruit *** is necessary to ensure proper crop ***,the effect of fertilization on salt tolerance in grapevine is *** this study,we investigated the effect of nitrogen fertilizer(0.01 and 0.1 mol L^(-1)NH_(4)NO_(3))application on the salt(200 mmol L^(-1)NaCl)tolerance of grapevine based on physiological indices,and transcriptomic and metabolomic *** results revealed that 0.01 mol L^(-1)NH_(4)NO_(3) supplementation significantly reduced the accumulation of superoxide anion(O_(2)^(-)·),enhanced the activities of superoxide dismutase(SOD)and peroxidase(POD),and improved the levels of ascorbic acid(AsA)and glutathione(GSH)in grape leaves compared to salt treatment ***,joint transcriptome and metabolome analyses showed that the differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were significantly enriched in the flavonoid biosynthesis pathway(ko00941)and the flavone and flavonol biosynthesis pathway(ko00944).In particular,the relative content of quercetin(C00389)was markedly regulated by salt and *** analysis revealed that exogenous foliar application of quercetin improved the SOD and POD activities,increased the AsA and GSH contents,and reduced the H_(2)O_(2) and O_(2)^(-)·***,10 hub DEGs,which had high Pearson correlations(R^(2)>0.9)with quercetin,were repressed by *** conclusion,all the results indicated that moderate nitrogen and quercetin application under salt stress enhanced the antioxidant system defense response,thus providing a new perspective for improving salt tolerance in grapes.

关键词： grapevine salt stress nitrogen multi-omics quercetin antioxidant

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