Metabolomic alterations and chromosomal instability status in gastric cancer

作     者：Cheng-Kun Tsai Ta-Sen Yeh Ren-Chin Wu Ying-Chieh Lai Meng-Han Chiang Kuan-Ying Lu Cheng-Yu Hung Hung-Yao Ho Mei-Ling Cheng Gigin Lin 

作者机构：Clinical Metabolomics Core LabChang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Department of Medical Imaging and InterventionImaging Core LabInstitute for Radiological ResearchChang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Chang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Department of PathologyChang Gung Memorial Hospital at Linkou and Chang Gung UniversityTaoyuan 333Taiwan Department of Medical Biotechnology and Laboratory ScienceCollege of MedicineChang Gung UniversityTaoyuan 333Taiwan Department of Biomedical ScienceCollege of MedicineChang Gung UniversityTaoyuan 333Taiwan 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2018年第24卷第33期

页      面：3760-3769页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by the Ministry of Science and Technology Taiwan grant,No.MOST 106-2314-B-182A-019-MY3 the Chang Gung Foundation,No.CMRPG3E1321-2,No.IRB201601916B0,and No.IRB103-7448B 

主　　题：Gastric cancer Metabolomics Oncogene Copy-number Chromosomal instability Liquid chromatography-mass spectrometry 

摘      要：AIM To explore the correlation of metabolomics profiles ofgastric cancer(GC) with its chromosomal instability(CIN) *** Nineteen GC patients were classified as CIN and nonCIN type by The Cancer Genome Atlas Research Group system, based on 409 oncogenes and tumor suppressor genes sequenced. The aqueous metabolites of the GC tumor and its surrounding adjacent healthy tissues were identified through liquid chromatographymass spectrometry. Groups were compared by defining variable importance in projection score of 1.2, a fold change value or its reciprocal of 1.2, and a P value of 0.05 as a significant *** In total,twelve men and seven women were enrolled, with a median age of 66 years(range, 47-87 years). The numbers of gene alterations in the CIN GC group were significantly higher than those in the non-CIN GC(32-218 vs 2-17; P 0.0005). Compared with the adjacent healthy tissues, GC tumors demonstrated significantly higher aspartic acid, citicoline, glutamic acid, oxidized glutathione, succinyladenosine, and uridine diphosphate-Nacetylglucosamine levels, but significantly lower butyrylcarnitine, glutathione hydroxyhexanoycarnitine, inosinic acid, isovalerylcarnitine, and threonine levels(all P 0.05). CIN tumors contained significantly higher phosphocholine and uridine 5 -monophosphate levels but significantly lower beta-citryl-L-glutamic acid levels than did non-CIN tumors(all P 0.05). CIN GC tumors demonstrated additional altered pathways involving alanine, aspartate, and glutamate metabolism, glyoxylate and dicarboxylate metabolism, histidine metabolism, and phenylalanine, tyrosine, and tryptophan *** Metabolomic profiles of GC tumors and the adjacent healthy tissue are distinct, and the CIN status is associated with downstream metabolic alterations in GC.